Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A25 F54 I1 R1 119 63.3 2848040 95.5% 2719878 127.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,969,880 T→C D554G (GAT→GGT)  U712_20470 ← Sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,969,8800TC97.2% 261.9 / NA 72D554G (GAT→GGT) U712_20470Sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (34/36);  minor base G (2/0);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTATCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCAATCGAGAACGTGACATGTCCGCCTT  >  CP006881/3969745‑3970012
                                                                                                                                       |                                                                                                                                    
acaggagTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  3:290590/135‑1 (MQ=255)
 gAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  7:28326/138‑1 (MQ=255)
 gAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  8:61678/138‑1 (MQ=255)
 gAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  5:289614/138‑1 (MQ=255)
 gAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  1:25390/138‑1 (MQ=255)
   agagTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCCTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCCGCCCGGTGCCCCCCAGCGTTAGCGTTAGCCCTTTacccaccc                                                                                                                                >  6:289507/1‑139 (MQ=255)
     agTAAATGTAATCGTCGTGGCTTTTCCTTATATGCTGTCCGCCCAAATATCTCCCCCCTGCGCCTGTTCCCATTACTTGGGAATGGCCCATCCCAGCCCCGTGCCCCCAAGCTTTCTCGGTCGCGCTTTacccacccgg                                                                                                                              >  6:34476/1‑137 (MQ=255)
     agTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGGAATCGCCAATCCCAGACCGGGGCCGCCCAGCGTTCGCGTTCTGGCGTTACccccccgg                                                                                                                              >  7:247730/1‑137 (MQ=255)
       tAAATGTAATCGTCGTGGCGTTTCCGTCTGTGCTGTCCGCGCAAATATCTCCCCAGGGCGCCGCTACCATTTACTTTGCAATCGCACAACCCACCCAGGTGCACCCCAACTTTCTAATTATAGCTTTAGCAACCCAGt                                                                                                                             >  6:294948/1‑138 (MQ=255)
               aTCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCACTCCCAGACCGGTGCCGCCAAGCGTTCTCGTTCTCCCTTTACCCACCCTGTAGAAGCCGt                                                                                                                    >  7:238892/1‑139 (MQ=255)
               aTCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   >  7:41345/1‑124 (MQ=255)
               aTCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  8:41345/124‑1 (MQ=255)
               aTCGTCGTGCCGTTTCCTTCTATGGTGTCCGCCCAAATATCTCCCCCGTGCGCCGGTACCATTTCCTTGGCAATCGCCAATCCCAGCCCGGTGCCGCCAAGCGTTCTGGTTCTGGCTTTAGCCACCCAGGAGAAGCTGt                                                                                                                    >  4:290590/1‑139 (MQ=255)
                      tGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGAc                                                                                                             <  2:127702/139‑1 (MQ=255)
                               cTTCTATGCTTTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGa                                                                                                     <  7:215036/138‑1 (MQ=255)
                                     tGCTGTCCGCCCTAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTGGTTCTCGCTTTACCCa                                                                                                                                   <  5:193248/102‑1 (MQ=255)
                                     tGCTGTCCGCCCTAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   >  6:193248/1‑102 (MQ=255)
                                         gTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTc                                                                                          >  2:15603/1‑139 (MQ=255)
                                          tCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAATCTGTCAAAGACTTTTTCGACATCTTTTTTCg                                                                                         >  4:320997/1‑139 (MQ=255)
                                          tCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCg                                                                                         >  7:90065/1‑139 (MQ=255)
                                            cGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCCCCCTGTAGAAGCGGTCAAAGACTTTTTCGACATGCTTTTTCgg                                                                                        >  6:297665/1‑138 (MQ=255)
                                            cGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGa                                                                                       <  5:270679/139‑1 (MQ=255)
                                            cGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACACACCCTGTAGAAGCGGTCAAAGACTTTTTCGAGCTCTTTTTTCgg                                                                                        >  3:200114/1‑138 (MQ=255)
                                             gCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGaa                                                                                      <  1:107583/139‑1 (MQ=255)
                                               ccAAAAATCTCCACCGAGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCCAGCTTTCTCGATCTCGCTTTTCCGACCCTGTAGAAGCGGTCATAGACTTTTTCGACATCTTTTATCGGAATg                                                                                    <  8:67239/139‑1 (MQ=255)
                                                    tatCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGAt                                                                               >  8:38688/1‑139 (MQ=255)
                                                          cACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATcc                                                                             <  7:148530/135‑1 (MQ=255)
                                                          cACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTc                                                                         <  5:285896/139‑1 (MQ=255)
                                                           aCCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCa                                                                        >  2:125065/1‑139 (MQ=255)
                                                             cGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATc                                                                      >  7:45175/1‑139 (MQ=255)
                                                               tGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCt                                                                    >  4:236861/1‑139 (MQ=255)
                                                               tGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCt                                                                    >  4:132686/1‑139 (MQ=255)
                                                               tGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCt                                                                    >  7:150803/1‑139 (MQ=255)
                                                               tGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCt                                                                    >  8:294664/1‑139 (MQ=255)
                                                                gcgcCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCtt                                                                   <  4:176848/139‑1 (MQ=255)
                                                                  gcCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCa                                                                 <  1:125065/139‑1 (MQ=255)
                                                                  gcCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCGTTACCCACCCTGTAGAAGCGGTCAAAGACGTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCa                                                                 <  8:247730/139‑1 (MQ=255)
                                                                    cTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACg                                                               <  4:215286/139‑1 (MQ=255)
                                                                    cTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACg                                                               <  8:45175/139‑1 (MQ=255)
                                                                    cTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACg                                                               <  5:34476/139‑1 (MQ=255)
                                                                    cTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACg                                                               <  5:290463/139‑1 (MQ=255)
                                                                    cTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACg                                                               <  5:180136/139‑1 (MQ=255)
                                                                            tttCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   >  2:349967/1‑63 (MQ=255)
                                                                            tttCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  1:349967/63‑1 (MQ=255)
                                                                            tttCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGa                                                                                >  3:130779/1‑114 (MQ=255)
                                                                            tttCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGa                                                                                <  4:130779/114‑1 (MQ=255)
                                                                              tCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGcc                                                                                  >  6:269293/1‑110 (MQ=255)
                                                                              tCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGcc                                                                                  <  5:269293/110‑1 (MQ=255)
                                                                              tCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGata                                                         >  7:253913/1‑135 (MQ=255)
                                                                              tCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGAtata                                                       >  1:245407/1‑137 (MQ=255)
                                                                                cTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATagag                                                    <  5:289507/138‑1 (MQ=255)
                                                                                cTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATagag                                                    <  1:15603/138‑1 (MQ=255)
                                                                                cTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATagag                                                    <  5:208468/138‑1 (MQ=255)
                                                                                cTTGGCAATCGCCAATCCCAGACCGGAGCCGACAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATagag                                                    <  1:118229/138‑1 (MQ=255)
                                                                                  tGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAg                                                 >  4:335300/1‑139 (MQ=255)
                                                                                   ggCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   >  7:49308/1‑56 (MQ=255)
                                                                                   ggCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  8:49308/56‑1 (MQ=255)
                                                                                     cAATCGCCAATCCCAGACAGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGAGATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGttc                                              <  7:281505/139‑1 (MQ=255)
                                                                                        tCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   >  6:289231/1‑51 (MQ=255)
                                                                                        tCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCa                                                                                                                                   <  5:289231/51‑1 (MQ=255)
                                                                                         cGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATGTTTTTTCGGTATGCCGATCACTTCATCCTTCCCGAGCATATAGAGAATttattac                                          >  7:305401/1‑137 (MQ=255)
                                                                                                    gACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCGTCGTCAttta                                >  3:212537/1‑136 (MQ=255)
                                                                                                    gACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGAc                               <  8:150803/139‑1 (MQ=255)
                                                                                                         gTGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCaa                          >  4:84755/1‑139 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGa                                                                                <  4:15920/84‑1 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGa                                                                                >  3:15920/1‑84 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCAAt                         >  4:163430/1‑139 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTACTTCCTCTTCATAGACATaaa                          >  3:357327/1‑138 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGCAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCATGGTCATTGACATCaa                          >  5:345686/1‑138 (MQ=255)
                                                                                                          tGCCGCCAAGCTTTCTCGGTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGCCTTTTTCGACATCGGTTTTCGGAATGCCGCTCCCGTCATCCGGCACGCGGATATAGAGAATTTATTACTGTTCCTTGACATCAAt                         >  8:135773/1‑139 (MQ=255)
                                                                                                                cAAGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCAATCGAGaa                   <  2:245407/139‑1 (MQ=255)
                                                                                                                 aaGCTTTCTCGTTCTCGCTTTACCCACCCTGTAGAAGCGGTCAAAGACGTTGTCGTCATCTTTTTTCGGATTGCCGAGACCTTGATACTTCACGATGATATAGATAAGTTCTTGCTGTTCATTGACATCAATCGTGaag                  >  7:257684/1‑138 (MQ=255)
                                                                                                                                 gCTTTACCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGACGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCATCTTCATTTACATAAATAGAGAACGTGACATGTCGGCCtt  >  6:33588/1‑139 (MQ=255)
                                                                                                                                   tttACCCACCCTGAAGAAGCGGTCAAAGTCTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATGCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCAATCGAGAACGTGACATGTCCGCCtt  <  5:17043/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GGAAGAGTAAATGTAATCGTCGTGCCTTTTCCTTCTATGCTGTCCGCCCAAATATCTCCACCGTGCGCCTGTACCATTTCCTTGGCAATCGCCAATCCCAGACCGGTGCCGCCAAGCTTTCTCGTTCTCGCTTTATCCACCCTGTAGAAGCGGTCAAAGACTTTTTCGACATCTTTTTTCGGAATGCCGATCCCTTCATCCTTCACGCTGATATAGAGAAGTTCTTCCTCTTCATTGACATCAATCGAGAACGTGACATGTCCGCCTT  >  CP006881/3969745‑3970012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A