Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F64 I0 R1	160	79.1	3727730	96.4%	3593531	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,172,857	A→T	26.2%	E562V (GAA→GTA)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,172,857	0	A	T	26.2%	124.0 / 54.5	65	E562V (GAA→GTA)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (27/21); new base T (11/6); total (38/27)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCT  >  CP009273/4172718‑4172993

gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                                                                                                          <  1:1137756/140‑1 (MQ=255)

gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                                                                                                          <  1:1742341/140‑1 (MQ=255)

gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                                                                                                          <  1:168783/140‑1 (MQ=255)

   cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAc                                                                                                                                       <  1:1592407/140‑1 (MQ=255)

      aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcccc                                                                                                                                    >  1:1633925/1‑140 (MQ=255)

           cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                                                                                                               >  1:359840/1‑140 (MQ=255)

           cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                                                                                                               >  1:31299/1‑140 (MQ=255)

           cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                                                                                                                               >  1:359843/1‑140 (MQ=255)

             cGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTCCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt                                                                                                                             >  2:576399/1‑140 (MQ=255)

                       gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAGGTTCGAGACGTACACCCGCCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                   <  1:16411/140‑1 (MQ=255)

                       gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                   <  1:245773/140‑1 (MQ=255)

                         ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                                                 <  1:1821576/140‑1 (MQ=255)

                         ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                                                 <  1:1172504/140‑1 (MQ=255)

                           aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                               <  1:1053593/140‑1 (MQ=255)

                           aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                               <  2:1470126/140‑1 (MQ=255)

                           aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                               <  1:1861695/140‑1 (MQ=255)

                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                              >  1:534508/1‑140 (MQ=255)

                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                              >  1:643094/1‑140 (MQ=255)

                                              ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg                                                                                            <  2:1487235/140‑1 (MQ=255)

                                                 cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                         >  2:1340494/1‑140 (MQ=255)

                                                 cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                         >  2:290053/1‑140 (MQ=255)

                                                 cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                         >  2:827948/1‑140 (MQ=255)

                                                  aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                                                                        <  2:1504701/140‑1 (MQ=255)

                                                  aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                                                                        <  2:1326536/140‑1 (MQ=255)

                                                  aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                                                                        <  2:88792/140‑1 (MQ=255)

                                                    tCGGCCCTGGCGGTGTGACCCGTGACCGTGCAGGCTTCGAAGTTCGAGAGGTACACCCGACTCACTAGGGTCGCTTATTTCAAATCGTAACCCATGACGGTCCGAACATCGGTGTGATCAACTCTCTGTCCGTTTAGGca                                                                                      <  1:1433127/140‑1 (MQ=255)

                                                    tCGGCCCAGGCGGTCTGACCCGTGAAGGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                      <  1:1482713/140‑1 (MQ=255)

                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                      <  1:1324730/140‑1 (MQ=255)

                                                      ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                                                                                                           <  2:109062/101‑1 (MQ=255)

                                                      ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                                                                                                           >  1:109062/1‑101 (MQ=255)

                                                       gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                                                <  2:1500193/127‑1 (MQ=255)

                                                       gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                                                >  1:1500193/1‑127 (MQ=255)

                                                        cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                                                                                  <  1:869390/140‑1 (MQ=255)

                                                        cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACACGACTCACTACGGTCGCGTATGTCCAAGCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTTTACGCACAGa                                                                                  <  1:68819/140‑1 (MQ=255)

                                                         ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                                                                                 >  1:1194173/1‑140 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        >  1:1837316/1‑140 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        >  1:775502/1‑140 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        >  2:183814/1‑140 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        <  2:359840/140‑1 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        <  2:31299/140‑1 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        <  2:359843/140‑1 (MQ=255)

                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                        >  2:86597/1‑140 (MQ=255)

                                                                         gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACggtcgt                                                                 >  2:502547/1‑136 (MQ=255)

                                                                           gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                                                                                                                                 <  1:186469/74‑1 (MQ=255)

                                                                           gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                                                                                                                                 >  2:186469/1‑74 (MQ=255)

                                                                           gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGACCATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt                                                               >  2:1574302/1‑140 (MQ=255)

                                                                                      cTTCGACGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                                                    <  2:89771/140‑1 (MQ=255)

                                                                                      cTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                                                    <  2:912515/140‑1 (MQ=255)

                                                                                                       tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                   <  2:156083/140‑1 (MQ=255)

                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTACAGTGACCGACg                                  >  2:579230/1‑140 (MQ=255)

                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg                                  >  2:854103/1‑140 (MQ=255)

                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg                                  >  2:353901/1‑140 (MQ=255)

                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg                                  >  2:1791767/1‑140 (MQ=255)

                                                                                                                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGTGACTGCGTCTCGTAAAGTGACCGACGGTGTTGTa                          >  1:989780/1‑140 (MQ=255)

                                                                                                                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTa                          >  1:1073706/1‑140 (MQ=255)

                                                                                                                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTa                          >  1:363196/1‑140 (MQ=255)

                                                                                                                 tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTaa                         >  1:350064/1‑140 (MQ=255)

                                                                                                                   aCTTCGGTCGCGTATGTCCAATCGAAACCCCTGATGGTCCGAACCTCGGTCTGATGAACTCTCTGTCCGTGTACGCCCAGACTAACGAATACGGCTGCCTGGAGACTCCGTATCGTAAAGTGACCGCCGGTGTTGTCACt                       >  2:291361/1‑140 (MQ=255)

                                                                                                                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                     >  1:287286/1‑140 (MQ=255)

                                                                                                                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                     >  1:1734107/1‑140 (MQ=255)

                                                                                                                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                     >  1:1436876/1‑140 (MQ=255)

                                                                                                                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                   >  2:200300/1‑38 (MQ=255)

                                                                                                                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                   <  1:200300/38‑1 (MQ=255)

                                                                                                                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTc             >  2:390784/1‑140 (MQ=255)

                                                                                                                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTc             >  1:148078/1‑140 (MQ=255)

                                                                                                                                   tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc       >  1:1291260/1‑140 (MQ=255)

                                                                                                                                   tCCAATCGAAACCCCGGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACCCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc       >  1:69433/1‑140 (MQ=255)

                                                                                                                                        tCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCt  >  1:71329/1‑140 (MQ=255)

GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCT  >  CP009273/4172718‑4172993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A