Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F64 I0 R1	416	84.5	3064920	96.6%	2960712	135.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,172,857	A→T	60.0%	E562V (GAA→GTA)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,172,857	0	A	T	60.0%	83.9 / 143.0	105	E562V (GAA→GTA)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (15/27); new base T (28/35); total (43/62)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 109 total aligned reads displayed.

CCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTC  >  CP009273/4172720‑4172992

ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCCCTACGGTCGCGTATGTCCAATCGtaa                                                                                                                                       >  1:1279505/1‑140 (MQ=255)

ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGtaa                                                                                                                                       >  1:25433/1‑140 (MQ=255)

ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGtaa                                                                                                                                       >  2:864114/1‑140 (MQ=255)

ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGtaa                                                                                                                                       >  1:444945/1‑140 (MQ=255)

  aGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAAcc                                                                                                                                     >  2:578369/1‑140 (MQ=255)

              tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCg                                                                                                                         >  1:1499203/1‑140 (MQ=255)

              tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCg                                                                                                                         >  2:1519422/1‑140 (MQ=255)

              tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                                                                                                         >  1:1057573/1‑140 (MQ=255)

              tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                                                                                                         >  1:1299382/1‑140 (MQ=255)

              tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                                                                                                         >  1:1202617/1‑140 (MQ=255)

                  tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACa                                                                                                                     <  1:187618/140‑1 (MQ=255)

                  tGAGAGTACGCACAAACGTCGGATCTCCGCCCTCGGCCCAGGCGGTCTGACCCGGGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCGGAAGGTCCGACCa                                                                                                                     <  2:326363/140‑1 (MQ=255)

                     gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                  <  1:961645/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:545804/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:758728/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:1359070/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:1359050/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:1359042/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  1:695437/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:820177/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  2:910216/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  1:194358/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  1:1157336/140‑1 (MQ=255)

                       ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                <  1:1446780/140‑1 (MQ=255)

                         aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                              <  2:338486/140‑1 (MQ=255)

                         aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                              <  1:1342512/140‑1 (MQ=255)

                         aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAATCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                              <  2:338658/140‑1 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTcc                                                                                                                          <  1:261633/127‑1 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTcc                                                                                                                          >  2:261633/1‑127 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTcc                                                                                                                          >  2:1343304/1‑127 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTcc                                                                                                                          <  1:1343304/127‑1 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  1:218366/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  1:1066435/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  2:834049/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  1:1235651/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  2:502139/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  2:1327165/1‑140 (MQ=255)

                          cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                             >  1:1483408/1‑140 (MQ=255)

                                 aCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                      >  2:436672/1‑140 (MQ=255)

                                 aCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                      >  2:1117287/1‑140 (MQ=255)

                                 aCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                      <  1:1246312/140‑1 (MQ=255)

                                   gtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct                                                                                                    <  2:1483408/140‑1 (MQ=255)

                                           tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                                                                            >  1:793865/1‑140 (MQ=255)

                                           tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                                                                            >  1:1205530/1‑140 (MQ=255)

                                             cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                                                          >  1:533501/1‑140 (MQ=255)

                                                    ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCAGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca                                                                                   >  1:236278/1‑140 (MQ=255)

                                                      cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                                                                                 <  1:578369/140‑1 (MQ=255)

                                                         aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                                                                              >  2:415565/1‑140 (MQ=255)

                                                         aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                                                                              >  2:428304/1‑140 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACTCACAGACTAACGAATa                                                                       >  2:1436005/1‑140 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaaaa                                                                       >  2:1501798/1‑138 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                       <  2:533501/140‑1 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                       <  2:1202617/140‑1 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                       <  2:1057573/140‑1 (MQ=255)

                                                                cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                       <  2:1299382/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGTCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTTATCAACTCTCTGTCGGTGTACGCACAGACTAACGAATACGGc                                                                   <  1:790637/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  2:443682/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  1:573471/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  2:884572/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  1:658250/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  1:631840/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  1:1019677/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGCTTCGAACTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCACTCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  2:1187652/140‑1 (MQ=255)

                                                                    cccGTGAACGTGCAGGAGTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                                                   <  2:1187672/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  2:1128170/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  1:908084/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCAGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  2:591411/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCTGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  2:1500064/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  1:250085/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  2:1124810/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  1:469599/140‑1 (MQ=255)

                                                                                  ggCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  2:117962/140‑1 (MQ=255)

                                                                                  ggCTTAGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                                                     <  1:1286313/140‑1 (MQ=255)

                                                                                       cGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                >  2:1332442/1‑140 (MQ=255)

                                                                                             tCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGTGACTCCGGATAGTAAAg                                          >  1:687617/1‑140 (MQ=255)

                                                                                             tCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg                                          >  1:1275198/1‑140 (MQ=255)

                                                                                                    gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGa                                   <  1:517763/140‑1 (MQ=255)

                                                                                                     tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                  <  1:1327165/140‑1 (MQ=255)

                                                                                                     tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                  <  2:793865/140‑1 (MQ=255)

                                                                                                     tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                  <  1:834049/140‑1 (MQ=255)

                                                                                                     tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                  <  2:549243/140‑1 (MQ=255)

                                                                                                     tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                  <  1:502139/140‑1 (MQ=255)

                                                                                                             aCTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttgt                          >  1:501486/1‑140 (MQ=255)

                                                                                                                    aCGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCg                                                                                                                  >  2:220226/1‑45 (MQ=255)

                                                                                                                     cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACg                  <  2:1278861/140‑1 (MQ=255)

                                                                                                                     cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACg                  <  2:1278842/140‑1 (MQ=255)

                                                                                                                     cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACg                  <  2:1511610/140‑1 (MQ=255)

                                                                                                                     cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACg                  <  1:258916/140‑1 (MQ=255)

                                                                                                                     cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACg                  <  2:1511645/140‑1 (MQ=255)

                                                                                                                        tCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa               >  1:1308881/1‑140 (MQ=255)

                                                                                                                        tCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa               >  1:809391/1‑140 (MQ=255)

                                                                                                                        tCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa               >  1:1515645/1‑140 (MQ=255)

                                                                                                                        tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa               >  2:891235/1‑140 (MQ=255)

                                                                                                                            gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCa           <  2:402819/140‑1 (MQ=255)

                                                                                                                            gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCa           <  2:1248264/140‑1 (MQ=255)

                                                                                                                            gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCa           <  2:402927/140‑1 (MQ=255)

                                                                                                                             tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACTAATACGGCTTCCTAGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCAc          >  2:1018969/1‑140 (MQ=255)

                                                                                                                             tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAACTTCAc          >  2:49354/1‑140 (MQ=255)

                                                                                                                             tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCAc          >  2:69831/1‑140 (MQ=255)

                                                                                                                                     aTCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTc  >  1:733242/1‑140 (MQ=255)

CCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTC  >  CP009273/4172720‑4172992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A