Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F64 I0 R1 160 79.1 3727730 96.4% 3593531 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,501 G→C 31.4% R1075P (CGT→CCT) ‡ rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,5010GC31.4% 129.5 / 86.4 83R1075P (CGT→CCT) ‡rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (27/30);  new base C (11/15);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCT  >  CP009273/4178366‑4178639
                                                                                                                                       |                                                                                                                                          
aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                        >  2:1427930/1‑140 (MQ=255)
aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                                                                        >  2:1674972/1‑140 (MQ=255)
  gTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGAACGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGc                                                                                                                                      <  2:1852183/140‑1 (MQ=255)
     aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGGCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGTTATGCGTCCGGCACt                                                                                                                                   >  2:1648495/1‑140 (MQ=255)
         gTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGaaa                                                                                                                               <  2:36415/140‑1 (MQ=255)
                aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTg                                                                                                                        <  2:1150703/140‑1 (MQ=255)
                aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTg                                                                                                                        <  1:717186/140‑1 (MQ=255)
                   cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATg                                                                                                                     <  1:493969/140‑1 (MQ=255)
                    tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTTGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGc                                                                                                                    >  2:344372/1‑140 (MQ=255)
                     ttACTGACCTGCTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:953956/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTGTTCGCTGGTGGTTCGGGATTCCGCAGAACGTACGGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:1728997/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:1860559/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:236967/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:712090/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:308504/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  1:348538/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:68423/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:427043/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1048772/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:107241/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1371744/140‑1 (MQ=255)
                     ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1743775/140‑1 (MQ=255)
                          gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                              <  1:1837302/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                         <  2:1154265/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                         <  2:1532938/140‑1 (MQ=255)
                               gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                         <  2:602054/140‑1 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1659579/129‑1 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   >  1:1659579/1‑129 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                        >  1:1614964/1‑140 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   <  2:1354662/129‑1 (MQ=255)
                                aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                   >  1:1354662/1‑129 (MQ=255)
                                                ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                        <  1:1216175/140‑1 (MQ=255)
                                                 tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                                                                       >  2:477137/1‑140 (MQ=255)
                                                  aCGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAc                                                                                      >  1:1064791/1‑140 (MQ=255)
                                                  aCGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAc                                                                                      >  1:713345/1‑140 (MQ=255)
                                                      gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCCGGGTAACGACGTTCTGATCCCAGGTCCCGag                                                                                  >  1:1675697/1‑139 (MQ=255)
                                                      gTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                                                                                  >  1:1675691/1‑140 (MQ=255)
                                                        cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                                >  1:21834/1‑140 (MQ=255)
                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTGGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:905299/140‑1 (MQ=255)
                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:1689387/140‑1 (MQ=255)
                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:150538/140‑1 (MQ=255)
                                                          gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGAGTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:776217/140‑1 (MQ=255)
                                                          gACCGACGAACTGACCGGTCGTTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGTTAAAGATATGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:856294/140‑1 (MQ=255)
                                                          gACCGACGAACGTACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                              <  2:1200698/140‑1 (MQ=255)
                                                           aCCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGcc                                                                             <  1:1164259/140‑1 (MQ=255)
                                                              gacgacATGACCGGTCTGTCTTAGCTGGTTGTTATGTAGTCTGCAGATCGTTCCGCTGGTGTTAAAGATCTGCCTCCTGCACTGAATATCGTTGATGCTCAGGGTAACGAAGTTCTGATCCCAGGTAACGATGTGCCAgg                                                                          <  2:884608/140‑2 (MQ=255)
                                                              gacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                                                                          <  2:1051079/140‑1 (MQ=255)
                                                               acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                         >  2:294560/1‑140 (MQ=255)
                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                        >  1:1019974/1‑140 (MQ=255)
                                                                cgaACTGACCGGTCTGTCTTCGCTGGTGGTGCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                        >  1:36276/1‑140 (MQ=255)
                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                             >  1:1672228/1‑140 (MQ=255)
                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                             >  1:1433516/1‑140 (MQ=255)
                                                                           gtctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGCGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTCCTTCCTg                                                             >  1:408953/1‑140 (MQ=255)
                                                                                 ctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                       <  1:792927/140‑1 (MQ=255)
                                                                                 ctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt                                                       <  1:1770477/140‑1 (MQ=255)
                                                                                       tggtggTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTCCCGGGTAAATCg                                                 >  2:957775/1‑140 (MQ=255)
                                                                                        ggttgtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGa                                                <  2:1383914/140‑1 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                              >  1:1693893/1‑140 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                              >  1:1027929/1‑140 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                              >  1:1194649/1‑140 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                              >  1:1224048/1‑140 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                     >  1:1050970/1‑133 (MQ=255)
                                                                                          tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                     <  2:1050970/133‑1 (MQ=255)
                                                                                             ttCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCAGCCGGGTAAAGCGATTGtt                                           <  1:821221/140‑1 (MQ=255)
                                                                                                       cGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCCGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAg                                 >  2:1293061/1‑140 (MQ=255)
                                                                                                       cGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAg                                 >  2:57697/1‑140 (MQ=255)
                                                                                                          agcaCGGACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGCTGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATCTGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAGTGTTCAGCTGGAAGATg                              <  1:1655905/137‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  1:254133/140‑1 (MQ=255)
                                                                                                          aGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATAGGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                              <  2:569988/140‑1 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                            >  1:66204/1‑140 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                            >  1:639779/1‑140 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                            >  1:1499653/1‑140 (MQ=255)
                                                                                                            aaCGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                            >  2:1416257/1‑140 (MQ=255)
                                                                                                                 aCCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACa                       >  2:284310/1‑140 (MQ=255)
                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                           >  2:1638582/1‑81 (MQ=255)
                                                                                                                        gtggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                           <  1:1638582/81‑1 (MQ=255)
                                                                                                                        gtggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTCAAGCGATTGTTCAGCTGGAAGATGGCGTACAGagcagc                >  2:1066602/1‑140 (MQ=255)
                                                                                                                        gtggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc                >  1:755642/1‑140 (MQ=255)
                                                                                                                        gtggtAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc                >  2:335060/1‑140 (MQ=255)
                                                                                                                         tggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct               >  2:651605/1‑140 (MQ=255)
                                                                                                                               aGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg         <  1:492966/140‑1 (MQ=255)
                                                                                                                               aGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg         <  1:477137/140‑1 (MQ=255)
                                                                                                                                      cGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCt  >  2:1756908/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
AAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCT  >  CP009273/4178366‑4178639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A