Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F54 I1 R1 6 159.9 7861600 93.2% 7327011 135.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 6,070,587 A→G G38G (GGT→GGC)  PP_5324 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,070,5870AG97.4% 754.8 / 3.7 231G38G (GGT→GGC) PP_5324two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (105/119);  total (107/124)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 231 total aligned reads displayed.

TGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACT  >  NC_002947/6070442‑6070732
                                                                                                                                                  |                                                                                                                                                 
tGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGGACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGt                                                                                                                                               >  1:3034605/1‑150 (MQ=255)
   tCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGa                                                                                                                                            <  1:3534725/150‑1 (MQ=255)
   tCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGa                                                                                                                                            >  2:3377897/1‑150 (MQ=255)
     aCCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                          <  1:1324346/150‑1 (MQ=255)
             gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCgtcgtc                                                                                                                                  <  2:862145/150‑1 (MQ=255)
             gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCgtcgtc                                                                                                                                  <  1:1494769/150‑1 (MQ=255)
                 tGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATg                                                                                                                              >  2:2594471/1‑150 (MQ=255)
                 tGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATg                                                                                                                              >  1:1446147/1‑150 (MQ=255)
                  gCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATgg                                                                                                                             >  2:1961915/1‑150 (MQ=255)
                  gCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATgg                                                                                                                             >  1:693972/1‑150 (MQ=255)
                  gCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATgg                                                                                                                             <  1:2641151/150‑1 (MQ=255)
                   cAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGC‑TTCCGGTGTTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGc                                                                                                                            <  1:1198272/150‑1 (MQ=255)
                      gTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATg                                                                                                                              <  1:875718/145‑1 (MQ=255)
                        tcttcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATAc                                                                                                                       >  1:2414842/1‑150 (MQ=255)
                          ttcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATAccc                                                                                                                     >  2:2788778/1‑150 (MQ=255)
                            cAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCgg                                                                                                                   <  2:694475/150‑1 (MQ=255)
                            cAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCgg                                                                                                                   <  1:2937660/150‑1 (MQ=255)
                             aGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCggg                                                                                                                  >  2:1562279/1‑150 (MQ=255)
                              gTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGa                                                                                                                 >  2:2939238/1‑150 (MQ=255)
                                gCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTAGTCGATGGCCATACCCGGGAAg                                                                                                               <  1:89405/150‑1 (MQ=255)
                                   ggCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATg                                                                                                                              <  2:2497666/132‑1 (MQ=255)
                                      tgctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTa                                                                                                         <  1:164609/150‑1 (MQ=255)
                                         tgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                          <  1:2223599/114‑1 (MQ=255)
                                          gACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                          <  2:3865903/113‑1 (MQ=255)
                                          gACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCa                                                                                                     <  1:3700095/150‑1 (MQ=255)
                                           aCGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAg                                                                                                    <  2:517033/150‑1 (MQ=255)
                                           aCGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAg                                                                                                    >  2:1657245/1‑150 (MQ=255)
                                                aCCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACt                                                                                               >  2:3218161/1‑150 (MQ=255)
                                                aCCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACt                                                                                               >  2:2302451/1‑150 (MQ=255)
                                                  cAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTg                                                                                             <  2:2894197/150‑1 (MQ=255)
                                                   aGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGc                                                                                            >  1:2036565/1‑150 (MQ=255)
                                                    ggTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCg                                                                                           <  1:1773370/150‑1 (MQ=255)
                                                       cAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGAc                                                                                        <  2:267396/150‑1 (MQ=255)
                                                         gCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACCGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGCCACTTGCGGGCCa                                                                                      <  2:354987/150‑1 (MQ=255)
                                                            aTTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGCCACTTGCTGACCatat                                                                                   <  2:3156728/150‑1 (MQ=255)
                                                            aTTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGAt                                                                                   <  1:1877751/150‑1 (MQ=255)
                                                               ccAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCa                                                                                      >  2:2101220/1‑144 (MQ=255)
                                                                cAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACg                                                                               >  2:3751128/1‑150 (MQ=255)
                                                                    ccAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATa                                                                           >  1:3471662/1‑150 (MQ=255)
                                                                        aCATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATACACg                                                                       <  1:2471277/150‑1 (MQ=255)
                                                                         cATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgg                                                                      <  1:3318536/150‑1 (MQ=255)
                                                                          aTTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                     <  1:1562279/150‑1 (MQ=255)
                                                                           tttCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgggg                                                                    >  2:149641/1‑150 (MQ=255)
                                                                           tttCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGAATTACGCAGACACTTGCGGACCAGATCACGGATAAACgggg                                                                    <  2:482374/150‑1 (MQ=255)
                                                                           tttCCGGCATTTACCAG‑TCGCACAGGACCAGTTCGAATTCTTCCTTGCCGAGCATGGACATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATagatcgga                                                                    >  1:1049331/1‑143 (MQ=255)
                                                                            ttCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGNGGc                                                                   <  2:2414842/150‑1 (MQ=255)
                                                                            ttCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGc                                                                   <  2:2158521/150‑1 (MQ=255)
                                                                            ttCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTACTTCACTTGCTGTTCCATGGCCTTGCGGAAGTTGGAAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGc                                                                   <  1:2788778/150‑1 (MQ=255)
                                                                               cGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGc                                                                                            <  1:2727114/122‑1 (MQ=255)
                                                                               cGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTc                                                                >  2:3099614/1‑150 (MQ=255)
                                                                                ggCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCa                                                               >  1:3214893/1‑150 (MQ=255)
                                                                                 gCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCAt                                                              >  2:1886512/1‑150 (MQ=255)
                                                                                 gCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCAt                                                              >  2:2639534/1‑150 (MQ=255)
                                                                                       cccAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGCCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGa                                                        >  1:131417/1‑150 (MQ=255)
                                                                                        ccAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACTCTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGAc                                                       <  2:2560600/150‑1 (MQ=255)
                                                                                           g‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAg                                                    >  1:2590604/1‑150 (MQ=255)
                                                                                                cacaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGAGCAGATCACGGATAAACGGGGCGTCATCCACGACCAGcaca                                                >  1:2551446/1‑150 (MQ=255)
                                                                                                 acaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAt                                               <  2:1339959/150‑1 (MQ=255)
                                                                                                 acaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAt                                               >  2:1257204/1‑150 (MQ=255)
                                                                                                 acaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACCACCAAACTCCCTGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAt                                               >  2:3013474/1‑150 (MQ=255)
                                                                                                 acaGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACACGGAACGGGCTCATCCATGTACCAGCGGGCCAGATCCCGGATAAACGGGGCGTCATCCACGACCAGCACAt                                               >  1:1404611/1‑150 (MQ=255)
                                                                                                  caGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACg                                                                               <  2:3432634/117‑1 (MQ=255)
                                                                                                  caGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACg                                                                               >  1:3432634/1‑117 (MQ=255)
                                                                                                  caGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAtt                                              >  1:3799058/1‑150 (MQ=255)
                                                                                                     gACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                     >  1:3650157/1‑60 (MQ=255)
                                                                                                     gACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                     <  2:3650157/60‑1 (MQ=255)
                                                                                                      aCCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACt                                          <  1:1961915/150‑1 (MQ=255)
                                                                                                       ccAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGc                                                                   >  1:2466270/1‑124 (MQ=255)
                                                                                                       ccAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGc                                                                   <  2:2466270/124‑1 (MQ=255)
                                                                                                       ccAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCAGGAAGGCATTACGc                                                                                                      >  1:464489/1‑89 (MQ=255)
                                                                                                         aGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTa                                       <  2:1733732/150‑1 (MQ=255)
                                                                                                          ggTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTAc                                      <  1:3775489/150‑1 (MQ=255)
                                                                                                          ggTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGCCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTAc                                      <  2:131417/150‑1 (MQ=255)
                                                                                                           gTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGcaca                                                >  1:1256772/1‑139 (MQ=255)
                                                                                                               aaCGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATc                                 >  1:1891704/1‑150 (MQ=255)
                                                                                                                  gCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGAt                              >  2:1874228/1‑150 (MQ=255)
                                                                                                                  gCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGAt                              >  2:1029672/1‑150 (MQ=255)
                                                                                                                    ttCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGc                                                                   <  1:315260/111‑1 (MQ=255)
                                                                                                                    ttCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATAc                            <  1:3279612/150‑1 (MQ=255)
                                                                                                                    ttCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGGCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATAc                            <  2:3092119/150‑1 (MQ=255)
                                                                                                                       cTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATa                             >  2:2549986/1‑146 (MQ=255)
                                                                                                                       cTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTcc                         >  1:1153461/1‑150 (MQ=255)
                                                                                                                       cTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTcc                         >  2:566607/1‑150 (MQ=255)
                                                                                                                        ttGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgggg                                                                    >  1:2208593/1‑106 (MQ=255)
                                                                                                                        ttGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACgggg                                                                    <  2:2208593/106‑1 (MQ=255)
                                                                                                                        ttGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                     >  1:2512397/1‑105 (MQ=255)
                                                                                                                        ttGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCt                        >  2:3309740/1‑150 (MQ=255)
                                                                                                                         tGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCtt                       <  1:498554/150‑1 (MQ=255)
                                                                                                                           ccGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCtttt                     <  2:3799058/150‑1 (MQ=255)
                                                                                                                            cGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                     >  1:2686773/1‑101 (MQ=255)
                                                                                                                            cGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                     <  2:2686773/101‑1 (MQ=255)
                                                                                                                                  tggccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATccc              <  1:612514/150‑1 (MQ=255)
                                                                                                                                     ccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGAc           <  1:2302451/150‑1 (MQ=255)
                                                                                                                                       atggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAAc                                                                        <  1:3499601/87‑1 (MQ=255)
                                                                                                                                       atggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAAc                                                                        >  2:3499601/1‑87 (MQ=255)
                                                                                                                                       atggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTa         <  1:1820967/150‑1 (MQ=255)
                                                                                                                                         ggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGACACTCCTTTTGAATCCCGa            <  1:732600/145‑1 (MQ=255)
                                                                                                                                           ccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGc                                                   >  2:320949/1‑104 (MQ=255)
                                                                                                                                            cTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGCAGGCATTACGCAGACAGTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTa    >  2:848801/1‑150 (MQ=255)
                                                                                                                                            cTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAGTCCCGACTAGCCTa    >  2:3398006/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAG‑TCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGCCTACT  >  NC_002947/6070442‑6070732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A