Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F41 I1 R1	7	51.2	2540408	92.9%	2360039	134.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,070,587	A→G	G38G (GGT→GGC)	PP_5324 ←	two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,070,587	0	A	G	100.0%	153.6 / NA	47	G38G (GGT→GGC)	PP_5324	two‑component system response regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/22); total (25/22)

CTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGC  >  NC_002947/6070441‑6070727

cTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCg                                                                                                                                           <  1:398040/150‑1 (MQ=255)

    tCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGttaa                                                                                                                                       <  1:562581/150‑3 (MQ=255)

      aCCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACCGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATa                                                                                                                                     >  2:694004/1‑150 (MQ=255)

           gatgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                >  1:1200085/1‑150 (MQ=255)

           gatgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCACACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtc                                                                                                                                >  1:457090/1‑150 (MQ=255)

             tgaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGcgtcgt                                                                                                                              >  1:958316/1‑150 (MQ=255)

              gaACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCgtcgtc                                                                                                                             >  2:1194106/1‑150 (MQ=255)

                 cTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          <  1:251889/150‑1 (MQ=255)

                           ttcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATAccc                                                                                                                >  1:552618/1‑150 (MQ=255)

                            tcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCg                                                                                                               <  2:1200085/150‑1 (MQ=255)

                            tcAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCg                                                                                                               <  1:454860/150‑1 (MQ=255)

                               gTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAGGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGa                                                                                                            >  2:1132231/1‑150 (MQ=255)

                                       tgctgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTa                                                                                                    >  1:567480/1‑150 (MQ=255)

                                          tgACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGc                                                                                                 >  2:269792/1‑150 (MQ=255)

                                           gACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCa                                                                                                <  1:481938/150‑1 (MQ=255)

                                                cACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCa                                                                                                       <  1:1013750/138‑1 (MQ=255)

                                                cACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCa                                                                                                       >  2:1013750/1‑138 (MQ=255)

                                                      gTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCgg                                                                                     <  1:868290/150‑1 (MQ=255)

                                                       tCAGCAGGTGCAAGCTAGACGGTTCCGGGTTGTCGCAGTCGCACGGGACCAGGTCGAACGCTTCCTTGCCGAGCATGTCCATGGCCTTGCGGCCGTTGATAACGTCGTTGATGGCCATTCCCGGGAAGGCACTACGCAGACACTTGCggg                                                                                    >  2:338849/1‑149 (MQ=255)

                                                          gCAATTCCAATCCAGACCTTTACACCATTTTTCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATTGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCa                                                                                 <  2:611811/150‑1 (MQ=255)

                                                                 cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          >  2:1049315/1‑102 (MQ=255)

                                                                 cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGAt                                                                                                                          <  1:1049315/102‑1 (MQ=255)

                                                                 cAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGACCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACg                                                                          >  2:1151136/1‑150 (MQ=255)

                                                                    gCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                           >  2:237426/1‑98 (MQ=255)

                                                                    gCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGa                                                                                                                           <  1:237426/98‑1 (MQ=255)

                                                                     ccAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATa                                                                      >  2:662335/1‑150 (MQ=255)

                                                                         aCATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACg                                                                  <  1:1194106/150‑1 (MQ=255)

                                                                           aTTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACggg                                                                <  2:958316/150‑1 (MQ=255)

                                                                                 ggCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCa                                                          <  2:929576/150‑1 (MQ=255)

                                                                                    aTTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATcc                                                       >  2:373536/1‑150 (MQ=255)

                                                                                    aTTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATcc                                                       >  2:387042/1‑150 (MQ=255)

                                                                                      ttCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCAc                                                     >  2:720513/1‑150 (MQ=255)

                                                                                         ccAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGAc                                                  <  1:720513/150‑1 (MQ=255)

                                                                                           aGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCa                                                <  1:387042/150‑1 (MQ=255)

                                                                                                 acaGGACCAGGTCGAACGCTTCCTTGCNGAGCATGGCCATNGCCTTGNGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACAt                                          >  1:1050788/1‑150 (MQ=255)

                                                                                                 acaGGACCAGGTCGAACGCTTCATTGCCGAGCAGGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACCCAGACACTCCCGGACCACATCACGGATAAACGGGACGGCATCCACCACCAGCACAt                                          >  2:265451/1‑150 (MQ=255)

                                                                                                        cAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACttt                                   >  2:380357/1‑150 (MQ=255)

                                                                                                          ggTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTAc                                 >  2:306590/1‑150 (MQ=255)

                                                                                                             cGAACGCTTCCTTGCCGAGCATGGCCATGGCCATGCGGCAGTAGAACACATCGGCGATGTCCATACCCGGGACGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCa                              <  1:285545/150‑1 (MQ=255)

                                                                                                               aaCGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATc                            >  1:681961/1‑150 (MQ=255)

                                                                                                                           ccGAGCATGGCCATTGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCtttt                <  1:890510/150‑1 (MQ=255)

                                                                                                                              aGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGaa             <  2:457090/150‑1 (MQ=255)

                                                                                                                              aGCATGGCCATGGCCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGaa             <  2:567480/150‑1 (MQ=255)

                                                                                                                                catggccatggcCTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATc           <  1:306590/150‑1 (MQ=255)

                                                                                                                                    gccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGa       >  1:146339/1‑150 (MQ=255)

                                                                                                                                     ccatggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGAc      >  2:914671/1‑150 (MQ=255)

                                                                                                                                         ggccTTGCGGCCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGc  <  2:681961/150‑1 (MQ=255)

CTGGTCACCATGATGAACTGCAGGTTCTTCAGTGCAGGCTGCTGACGGCACCAGGTCAGCAATTCCAAGCCAGACATTTCCGGCATTTCCCAGTCGCACAGGACCAGGTCGAACGCTTCCTTGCCGAGCATGGCCATGGCCTTGCGACCGTTGATAGCGTCGTCGATGGCCATACCCGGGAAGGCATTACGCAGACACTTGCGGACCAGATCACGGATAAACGGGGCGTCATCCACGACCAGCACATTCACTTTACTCATCGATACTCCTTTTGAATCCCGACTAGC  >  NC_002947/6070441‑6070727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A