Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F80 I0 R1 1148 65.4 3296958 93.8% 3092546 131.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 196,351 C→G 17.6% T619T (ACC→ACG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947196,3510CG17.6% 226.2 / 41.5 91T619T (ACC→ACG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base G (7/9);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

GACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCC  >  NC_002947/196204‑196497
                                                                                                                                                    |                                                                                                                                                  
gACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAt                                                                                                                                                  <  1:1416821/150‑1 (MQ=255)
 aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                                                                                 >  1:24649/1‑150 (MQ=255)
 aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                                                                                 >  1:1630081/1‑150 (MQ=255)
  ccACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTa                                                                                                                                                >  1:384890/1‑150 (MQ=40)
      gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGt                                                                                                                                            >  1:480222/1‑150 (MQ=40)
       aTCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGtt                                                                                                                                           <  2:908091/150‑1 (MQ=40)
          aaCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGaa                                                                                                                                        <  2:1186494/150‑1 (MQ=40)
          aaCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGaa                                                                                                                                        <  1:1311029/150‑1 (MQ=255)
             gACTCAGTCGATATCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCCCCGAGGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACCCCGGTGACAGTGACCCTGTCTAACGGCCAAACCATTACCGTTGAAggc                                                                                                                                     >  2:152408/1‑148 (MQ=11)
              aCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                    <  2:760847/150‑1 (MQ=40)
               ttCGGTCGACACCACGACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                   <  2:347973/149‑1 (MQ=37)
               cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                   <  2:164598/150‑1 (MQ=40)
               cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCgg                                                                                                                                   <  1:466296/150‑1 (MQ=40)
                   gTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCaag                                                                                                                               >  1:169943/1‑149 (MQ=255)
                       acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGtt                                                                                                                                           <  1:1150071/134‑1 (MQ=39)
                       acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGtt                                                                                                                                           >  2:1150071/1‑134 (MQ=39)
                          ccacTACCGCGACCCTGCCGGCGAGCCCGTCGGTCACCGAA‑ACAGCGACAGCAAGTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGATGGCACTTACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                        >  1:1532989/1‑150 (MQ=11)
                            accaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGc                                                                                                                      >  1:1362873/4‑150 (MQ=2)
                            accaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGc                                                                                                                      >  1:531561/4‑150 (MQ=1)
                             cTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCa                                                                                                                     <  2:384890/150‑1 (MQ=14)
                             cTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCa                                                                                                                     >  1:1581008/1‑150 (MQ=14)
                              tACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAg                                                                                                                    >  1:931361/1‑150 (MQ=11)
                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGt                                                                                                                 >  2:1112206/1‑150 (MQ=35)
                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGt                                                                                                                 <  1:1136561/150‑1 (MQ=255)
                                       ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAttt                                                                                                           >  1:1234338/1‑150 (MQ=35)
                                         tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc                                                                                                         <  1:1112206/150‑1 (MQ=34)
                                         tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTcc                                                                                                         >  1:307514/1‑150 (MQ=11)
                                            cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAg                                                                                                                         >  1:812702/1‑131 (MQ=255)
                                            cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAg                                                                                                                         <  2:812702/131‑1 (MQ=255)
                                                     cGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                             >  1:351522/1‑150 (MQ=35)
                                                     cGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                             >  1:579644/1‑150 (MQ=35)
                                                     cGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                             >  2:1305677/1‑150 (MQ=35)
                                                       tCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAt                                                                                           <  1:77801/150‑1 (MQ=11)
                                                             aCCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc                                                                                     <  2:1234338/150‑1 (MQ=35)
                                                             aCCGAAGGCGGCGTGATA‑ACTTACCCCGTGACCGTTAGCAATCCTGACCAGACGCCGGTGATGTTCTCTTCCGCCAACGGCCAAACCATTCCCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc                                                                                     <  1:28957/150‑1 (MQ=11)
                                                               cGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGa                                                                                         <  2:1511760/144‑1 (MQ=35)
                                                               cGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGa                                                                                         >  1:1511760/1‑144 (MQ=35)
                                                               cGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCAATACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                   >  1:1240656/1‑150 (MQ=21)
                                                               cGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGAcccc                                                                                                  <  1:1154119/135‑1 (MQ=11)
                                                               cGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGAcccc                                                                                                  >  2:1154119/1‑135 (MQ=11)
                                                                 aaGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCg                                                                                                                  <  2:384325/117‑1 (MQ=255)
                                                                 aaGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCg                                                                                                                  >  1:384325/1‑117 (MQ=11)
                                                                     cggcgTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTAcaacaa                                                                             <  2:480222/150‑1 (MQ=34)
                                                                     cggcgTGATC‑ACACACCCCCTGAGCCGCTGTACCCCTGCCCAGACGCCGGTGACCGTGACCCCGTCCAACGGCCAAGCGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACGTCCAGACCCCGGCGAATGACGTCTAcaacaa                                                                             <  2:615614/150‑1 (MQ=11)
                                                                      ggcgTGATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACACCGGCGAATGACGTCTACaacaac                                                                            <  2:1362873/150‑1 (MQ=11)
                                                                        cgTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGCCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGCCGTCTACAACAACgg                                                                          <  1:1201528/150‑1 (MQ=21)
                                                                           gATC‑ACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTc                                                                       <  2:1160714/150‑1 (MQ=11)
                                                                            aTC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                        >  2:580132/1‑132 (MQ=37)
                                                                            aTC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                        <  1:580132/132‑1 (MQ=35)
                                                                            aTC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                        >  2:579963/1‑132 (MQ=37)
                                                                            aTC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                        <  1:579963/132‑1 (MQ=37)
                                                                             tC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa                                                                     <  2:659859/150‑1 (MQ=35)
                                                                                  ttACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                           >  1:1431796/1‑140 (MQ=33)
                                                                                  ttACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                           <  2:1431796/140‑1 (MQ=33)
                                                                                  ttACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                        <  2:259908/143‑1 (MQ=33)
                                                                                  ttACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                        >  1:259908/1‑143 (MQ=33)
                                                                                     cacCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCa                                                                                                                          <  2:601454/90‑1 (MQ=35)
                                                                                     cacCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCa                                                                                                                          >  1:601454/1‑90 (MQ=33)
                                                                                      cccGTCACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTAACCCTCTCCAATGACCAAACCATTACCATTGAAGTCGGCAAAACCCAAGGCAGCGTCGATTTCCACACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgt                                                             <  2:1232460/149‑1 (MQ=11)
                                                                                              ccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc                                                                                     >  1:486391/1‑118 (MQ=33)
                                                                                              ccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc                                                                                     <  2:486391/118‑1 (MQ=33)
                                                                                                tGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCg                                                   >  1:763087/1‑150 (MQ=39)
                                                                                                     aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAAc                                              <  1:106266/150‑1 (MQ=39)
                                                                                                       tCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGc                                            >  1:1124717/1‑150 (MQ=39)
                                                                                                       tCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGtt                                                                        <  2:508906/122‑1 (MQ=17)
                                                                                                          tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTGTACAACAACGGTTCGACTGTCAGTGTAACCATCGAGAACGCCAc                                         >  2:442414/1‑150 (MQ=255)
                                                                                                            cccAGACGCCGGTGACAGTGACCCGGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg                                                                                                                        >  1:312482/1‑69 (MQ=255)
                                                                                                            cccAGACGCCGGTGACAGTGACCCGGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg                                                                                                                        <  2:312482/69‑1 (MQ=255)
                                                                                                               aGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcg                                    <  2:579644/150‑1 (MQ=40)
                                                                                                                gACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcgg                                   <  1:92025/150‑1 (MQ=255)
                                                                                                                   gCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCAGTaa                                >  1:1113431/1‑150 (MQ=255)
                                                                                                                       gtgaccgtgaccCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACaacaac                                                                            >  2:97697/1‑102 (MQ=11)
                                                                                                                       gtgaccgtgaccCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACaacaac                                                                            <  1:97697/102‑1 (MQ=11)
                                                                                                                          aCAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                         <  1:425947/150‑1 (MQ=255)
                                                                                                                           cAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGc                        >  2:1288570/1‑150 (MQ=40)
                                                                                                                            aGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                         >  1:800195/1‑148 (MQ=40)
                                                                                                                            aGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                         <  2:800195/148‑1 (MQ=40)
                                                                                                                                aCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTg                   >  2:565416/1‑150 (MQ=38)
                                                                                                                                   cTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAg                                                                                                                    <  2:170469/50‑1 (MQ=40)
                                                                                                                                   cTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAg                                                                                                                    >  1:170469/1‑50 (MQ=40)
                                                                                                                                     gTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgt                                                             <  2:507332/103‑1 (MQ=35)
                                                                                                                                     gTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgt                                                             >  1:507332/1‑103 (MQ=35)
                                                                                                                                        cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGaa           <  1:1305677/150‑1 (MQ=39)
                                                                                                                                             gCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGa      >  2:192601/1‑150 (MQ=255)
                                                                                                                                              ccAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAAttt                             <  2:288121/126‑1 (MQ=40)
                                                                                                                                              ccAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAAttt                             >  1:288121/1‑126 (MQ=40)
                                                                                                                                              ccAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGc                        >  1:764314/1‑131 (MQ=39)
                                                                                                                                              ccAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGc                        <  2:764314/131‑1 (MQ=39)
                                                                                                                                               cAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                        <  2:290277/82‑1 (MQ=35)
                                                                                                                                               cAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                        >  1:290277/1‑82 (MQ=35)
                                                                                                                                               cAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACg    >  2:1320522/1‑150 (MQ=255)
                                                                                                                                                 aaacATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCCGACCCCGGCGAGTGACGTCTACAACAACGGTCCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGcc  <  1:431233/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATC‑ACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCC  >  NC_002947/196204‑196497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A