Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F80 I0 R1 1148 65.4 3296958 93.8% 3092546 131.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,348,749 A→G 58.8% intergenic (‑70/+404) PP_3820 ← / ← galU group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,348,7490AG58.8% 35.0 / 108.5 104intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (19/24);  new base G (31/30);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC  >  NC_002947/4348633‑4348896
                                                                                                                    |                                                                                                                                                    
ttCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTg                                                                                                                     <  1:270398/150‑1 (MQ=32)
      aTTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGc                                                                                                              <  2:250859/150‑1 (MQ=21)
      aTTACGGTTACCAACGAGACGGCGACGCCCAATGGCCGCCTGATCTTTGCTGCCCTTGGTGGCGCGGTTACCCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGc                                                                                                              <  1:365401/150‑1 (MQ=11)
       ttACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCg                                                                                                             <  2:121890/150‑1 (MQ=35)
          cGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTAc                                                                                                          <  2:1488624/150‑1 (MQ=25)
          cGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGCCCCTTGGTGGCCCGGTTAACCGACACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGGAGC‑TAGCGTAc                                                                                                          >  2:109691/1‑150 (MQ=11)
           ggTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACa                                                                                                         >  1:464451/1‑150 (MQ=35)
                 cAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGAtctc                                                                                                   >  2:1237167/1‑150 (MQ=25)
                  aaCGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGAtctac                                                                                                  <  2:464451/150‑3 (MQ=25)
                    cGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCa                                                                                                <  2:1093749/150‑1 (MQ=35)
                     gagaCGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAg                                                                                               >  1:183144/1‑150 (MQ=25)
                      agaCGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAgg                                                                                              <  2:183144/150‑1 (MQ=25)
                        aCGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGt                                                                                            >  2:245175/1‑150 (MQ=35)
                           gCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACATCTGCACGCTCGTCCCGTCGCGTTTCGCCAAAGTGTTGCACATGGTG‑AAGCGTACAGATCTCCCAGGGTAAt                                                                                         >  1:365228/1‑150 (MQ=11)
                               cGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgc                                                                                     <  2:952598/150‑1 (MQ=35)
                                 ccTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                                   >  2:838275/1‑150 (MQ=25)
                                 ccTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACATTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                                   <  1:1468903/150‑1 (MQ=17)
                                 ccTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                                   <  2:637206/150‑1 (MQ=35)
                                  cTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAgg                                                                                              >  2:335005/1‑138 (MQ=25)
                                  cTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgcg                                                                                  >  2:461379/1‑150 (MQ=25)
                                  cTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgcg                                                                                  >  2:463477/1‑150 (MQ=35)
                                  cTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTcgcgcg                                                                                  >  1:994902/1‑150 (MQ=34)
                                   tACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑aa                                                                                                                >  2:936059/1‑119 (MQ=33)
                                   tACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTCACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑aa                                                                                                                <  1:936059/119‑1 (MQ=18)
                                        gcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTg                                                                                                                     <  2:415563/110‑1 (MQ=17)
                                        gcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTg                                                                                                                     >  1:415563/1‑110 (MQ=17)
                                        gcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTc                                                                            >  1:932447/1‑150 (MQ=25)
                                          ggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCt                                                                          >  2:167455/1‑150 (MQ=25)
                                          ggcAGAATCTGTTAGGCCCTTGGTGGCGCGGTTAACCGCCACATACTACGGCTTCGGCTTACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGAGAC‑AAGGGTACAGATATCCGAGGGTTATGCGCGCGAcaatatt                                                                          >  2:312490/1‑144 (MQ=11)
                                             aTAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCt                                                                       >  1:1197528/1‑150 (MQ=25)
                                             aTAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTa                                                                                                           >  2:716579/1‑114 (MQ=38)
                                             aTAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTa                                                                                                           <  1:716579/114‑1 (MQ=38)
                                              tAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCtt                                                                      <  1:1238092/150‑1 (MQ=25)
                                              tAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCACGGCGCCTTTCGACGCGCGGTAG‑ACACCCGCGAAGAGGACGGATCTGCCAGGGTAATTCGCGCGACCTTCCTGCtt                                                                      >  1:365543/1‑150 (MQ=11)
                                               aaCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTa                                                                     <  1:559086/150‑1 (MQ=35)
                                               aaCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTa                                                                     <  2:866511/150‑1 (MQ=35)
                                                aCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAt                                                                    >  2:87079/1‑150 (MQ=21)
                                                aCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAt                                                                    <  1:886391/150‑1 (MQ=25)
                                                 ccTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATg                                                                   >  2:567786/1‑150 (MQ=25)
                                                  cTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCGTTTCGACGCGCGGTAGCACAGTTGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGc                                                                  >  2:162710/1‑150 (MQ=21)
                                                     ttCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTg                                                               >  2:674576/1‑150 (MQ=25)
                                                      tCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGt                                                              <  2:932447/150‑1 (MQ=25)
                                                       cGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTc                                                             <  1:567786/150‑1 (MQ=25)
                                                        ggCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCg                                                            >  1:1374872/1‑150 (MQ=25)
                                                        ggCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCg                                                            >  1:1150635/1‑150 (MQ=25)
                                                            cTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTcc                                                                                                  <  2:104184/108‑1 (MQ=38)
                                                            cTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTcc                                                                                                  >  1:104184/1‑108 (MQ=38)
                                                            cTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGAc                                                                                >  1:1089680/1‑126 (MQ=37)
                                                            cTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGAc                                                                                <  2:1089680/126‑1 (MQ=37)
                                                                   gcgcGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                       <  1:442772/81‑1 (MQ=14)
                                                                   gcgcGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAg                                                                                                                       >  2:442772/1‑81 (MQ=14)
                                                                     gcgGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTcaca                                               >  1:1590281/1‑150 (MQ=25)
                                                                     gcgGTTAACCGCCACTTACTACGGCTTCGGCTGACCTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTca                                                 <  1:504783/148‑1 (MQ=25)
                                                                     gcgGTTAACCGCCACTTACTACGGCTTCGGCTGACCTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTca                                                 >  2:504783/1‑148 (MQ=25)
                                                                       ggTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCACGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCCCGCGACCTTCCTCCTTATGCCTGTCGGATCTACGTCACAGc                                             >  2:817818/1‑150 (MQ=255)
                                                                            aCCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTcc                                        <  1:1477500/150‑1 (MQ=25)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAggg                                                                                             >  1:1033108/1‑96 (MQ=37)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTAc                                                     <  1:1517220/136‑1 (MQ=37)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTAc                                                     >  2:1517220/1‑136 (MQ=21)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTg                                                                                                                     >  2:538115/1‑73 (MQ=34)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTg                                                                                                                     <  1:538115/73‑1 (MQ=34)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑aa                                                                                                                <  1:881810/77‑1 (MQ=35)
                                                                             ccGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑aa                                                                                                                >  2:881810/1‑77 (MQ=35)
                                                                              cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGt                                                              >  2:724175/1‑126 (MQ=37)
                                                                              cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGt                                                              <  1:724175/126‑1 (MQ=37)
                                                                              cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGt                                      >  1:1438535/1‑150 (MQ=25)
                                                                                ccACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGt                                      >  2:669051/1‑148 (MQ=25)
                                                                                ccACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGt                                      <  1:669051/148‑1 (MQ=25)
                                                                                ccACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGc                                    <  1:313113/150‑1 (MQ=25)
                                                                                  aCTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCCACGTCACAGCGTTCCGTGCaa                                  <  2:101146/150‑1 (MQ=25)
                                                                                         acGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTgg                           <  1:1237167/150‑1 (MQ=25)
                                                                                             cgatctcTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTACCACAGTGGC‑AAGCGTACATATCTCCCAGAGTAATCCGCGCGACCTTCCTGCTTATGCCTGTCGGCTCTACGTCACAGCGTTCCGTGCAAGTATTGGGCtt                       <  1:249080/144‑1 (MQ=11)
                                                                                             cTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCtt                       <  2:1374872/150‑1 (MQ=25)
                                                                                               tCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTg                     <  1:586519/150‑1 (MQ=25)
                                                                                                cGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTgg                    <  1:87079/150‑1 (MQ=25)
                                                                                                cGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTgg                    <  1:167455/150‑1 (MQ=25)
                                                                                                cGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTgg                    <  2:854604/150‑1 (MQ=35)
                                                                                                 ggCTGCCTTCTGCACGCGCGTCCCGGCCCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCCCAGCGTTCCGTGCAAGTATTGGGCTTTGGc                   <  1:304786/150‑1 (MQ=11)
                                                                                                  gCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAtt                             <  2:1020383/139‑1 (MQ=37)
                                                                                                  gCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAtt                             >  1:1020383/1‑139 (MQ=37)
                                                                                                   cTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGCTCATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGa                 <  1:109691/150‑1 (MQ=255)
                                                                                                    tGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGAt                >  1:211340/1‑150 (MQ=25)
                                                                                                    tGACTTCTGCACGCTCGTACCGTCGCCTGTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTACGCCTGTCGGGTCTACGTCACAGCGTTACGTGCAATTAGTGGGCTTTAGctcg                >  2:249080/1‑147 (MQ=11)
                                                                                                      aCTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGAttt              >  1:868777/1‑150 (MQ=25)
                                                                                                       cTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtg             >  1:1121336/1‑150 (MQ=25)
                                                                                                        ttCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt            <  2:1197528/150‑1 (MQ=25)
                                                                                                        ttCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGc                                                                  <  2:614681/96‑1 (MQ=39)
                                                                                                        ttCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGc                                                                  >  1:614681/1‑96 (MQ=39)
                                                                                                        ttCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt            >  2:1320596/1‑150 (MQ=35)
                                                                                                        ttCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt            <  1:463477/150‑1 (MQ=35)
                                                                                                         tCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTggg                          <  2:323925/135‑1 (MQ=37)
                                                                                                         tCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTggg                          >  1:323925/1‑135 (MQ=37)
                                                                                                         tCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGAATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGTCGATTtgtg           >  2:1279254/1‑150 (MQ=21)
                                                                                                          cTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATTCCTGTCGGATCTACGTCACTGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGc          <  2:306128/150‑1 (MQ=25)
                                                                                                           tGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGcc         <  2:1438535/150‑1 (MQ=25)
                                                                                                             cACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTAGGATCTACTTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAc       >  2:708038/1‑150 (MQ=255)
                                                                                                             cACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGCC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAc       <  2:1121336/150‑1 (MQ=255)
                                                                                                                gCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGc                                                                  >  1:1353601/1‑88 (MQ=255)
                                                                                                                gCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGc                                                                  <  2:1353601/88‑1 (MQ=255)
                                                                                                                  tCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAc  >  2:161238/1‑150 (MQ=34)
                                                                                                                    |                                                                                                                                                    
TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC‑AAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC  >  NC_002947/4348633‑4348896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A