Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F80 I0 R1 1105 125.4 6144758 94.6% 5812941 133.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 550,760 G→C 38.3% G33R (GGT→CGT)  rpsJ → 30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947550,7600GC38.3% 62.0 / 117.7 95G33R (GGT→CGT) rpsJ30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base C (22/14);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-02

Only 100 of 101 total aligned reads displayed.

TTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCAGCCAACGGATA  >  NC_002947/550613‑550907
                                                                                                                                                   |                                                                                                                                                   
ttttAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGt                                                                                                                                                   >  2:1916727/1‑150 (MQ=255)
     gCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAACTATACCATTCCGTATAAGGAGAAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGcaca                                                                                                                                              >  1:2677590/1‑150 (MQ=255)
     gCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCCACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGcaca                                                                                                                                              >  1:2489925/1‑150 (MQ=255)
        ggCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGt                                                                                                                                           <  2:2915896/150‑1 (MQ=255)
            tAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGt                                                                                                                                       <  1:1889201/150‑1 (MQ=255)
             aGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTg                                                                                                                                      <  2:476304/150‑1 (MQ=255)
             aGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTg                                                                                                                                      <  2:1443500/150‑1 (MQ=255)
              gTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTgg                                                                                                                                     <  2:2489925/150‑1 (MQ=255)
              gTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAATTGCGTgg                                                                                                                                     <  2:2282823/150‑1 (MQ=255)
              gTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTgg                                                                                                                                     >  1:2282823/1‑150 (MQ=255)
                gCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTc                                                                                                                                   >  2:1444976/1‑150 (MQ=255)
                gCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTc                                                                                                                                   <  1:1942736/150‑1 (MQ=255)
                        tGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCa                                                                                                                           <  2:574058/150‑1 (MQ=255)
                          ggAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     >  2:126376/1‑122 (MQ=255)
                          ggAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     <  1:126376/122‑1 (MQ=255)
                           gAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTg                                                                                                                        <  2:590733/150‑1 (MQ=255)
                           gAACAGCCTGGAGTCTGAAATCCAATGAAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCAAAAGTGCGTGGTCAAAATCAACTg                                                                                                                        >  1:1403673/1‑150 (MQ=255)
                            aaCAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGc                                                                                                                       <  1:90674/150‑1 (MQ=255)
                             aCAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCACTTACACTGcc                                                                                                                      >  1:636712/1‑150 (MQ=255)
                               aGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTa                                                                                                                    >  1:2355305/1‑150 (MQ=255)
                                  cTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTAccc                                                                                                                 <  1:1612261/150‑1 (MQ=255)
                                     gAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCa                                                                                                              >  1:2505561/1‑150 (MQ=255)
                                       gtacgAAAGCAAATGCAAAATCAGCAAATCCGTATAAGGTTGAAGGCTATCGACCATCACCTGATCGACCAATCCACCCAGCAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCaaa                                                                                                            <  1:761184/146‑1 (MQ=255)
                                        tCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     >  1:1508648/1‑108 (MQ=255)
                                        tCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     <  2:1508648/108‑1 (MQ=255)
                                         cTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAaga                                                                                                          <  2:2505561/150‑1 (MQ=255)
                                          tGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     <  1:1374083/106‑1 (MQ=255)
                                          tGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTg                                                                                                                                                     >  2:1374083/1‑106 (MQ=255)
                                          tGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAagag                                                                                                         >  1:452207/1‑150 (MQ=255)
                                           gAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGc                                                                                                        >  1:174034/1‑150 (MQ=255)
                                            aaaTCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCg                                                                                                       >  2:1081521/1‑150 (MQ=255)
                                             aaTCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGt                                                                                                      <  1:1916727/150‑1 (MQ=255)
                                              aTCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGtt                                                                                                     <  2:1075736/150‑1 (MQ=255)
                                              aTCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGtt                                                                                                     <  2:2461599/150‑1 (MQ=255)
                                                 cAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCa                                                                                                  >  2:555231/1‑150 (MQ=255)
                                                  aaTGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCAc                                                                                                 >  1:1120206/1‑150 (MQ=255)
                                                    tGCACAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCg                                                                                               >  2:1404290/1‑150 (MQ=255)
                                                    tGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCg                                                                                               >  1:2027883/1‑150 (MQ=255)
                                                    tGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACACAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCg                                                                                               >  1:507069/1‑150 (MQ=255)
                                                     gCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTa                                                                                                                    <  1:156483/128‑1 (MQ=255)
                                                     gCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTa                                                                                                                    >  2:156483/1‑128 (MQ=255)
                                                        aaaTCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTCCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCt                                                                                           >  1:2250554/1‑150 (MQ=255)
                                                        aaaTCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCt                                                                                           <  2:2885121/150‑1 (MQ=255)
                                                                    ccGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCa                                                                               <  2:1120206/150‑1 (MQ=255)
                                                                         tCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGt                                                                                        <  1:1124257/136‑1 (MQ=255)
                                                                         tCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGt                                                                                        >  2:1124257/1‑136 (MQ=255)
                                                                         tCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTca                                                                          >  2:2674119/1‑150 (MQ=255)
                                                                             gTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTcaacaa                                                                      <  2:1835015/150‑1 (MQ=255)
                                                                             gTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGCTCTGGTCTcccc                                                                                  <  2:1721338/138‑1 (MQ=255)
                                                                             gTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGAGTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTcccc                                                                                  >  1:1721338/1‑138 (MQ=255)
                                                                             gTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTc                                                                           <  1:875943/145‑1 (MQ=255)
                                                                             gTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTc                                                                           >  2:875943/1‑145 (MQ=255)
                                                                                gAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGTTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGa                                                                   >  2:634991/1‑150 (MQ=255)
                                                                                gAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGCGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGtctc                                                                                     <  1:2311894/132‑1 (MQ=255)
                                                                                gAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGCGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGtctc                                                                                     >  2:2311894/1‑132 (MQ=255)
                                                                                gAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGa                                                                   >  2:2225730/1‑150 (MQ=255)
                                                                                  aGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGAcg                                                                 >  2:251747/1‑150 (MQ=255)
                                                                                    gCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGAcgcg                                                               <  1:555231/150‑1 (MQ=255)
                                                                                      tttCGACCATCGCCTGATCTACCAATCCACCCAGGAAATCGTGGAGACCTCGAAACGTACTGGTGCACAAGTGCGGGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGAcgcgcg                                                             >  1:2577068/1‑150 (MQ=255)
                                                                                      tttCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGAcgcgcg                                                             >  1:320000/1‑150 (MQ=255)
                                                                                      tttCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTcaaca                                                                       <  1:974377/140‑1 (MQ=255)
                                                                                          gACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCg                                                                                                       >  2:1588025/1‑104 (MQ=255)
                                                                                          gACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCg                                                                                                       <  1:1588025/104‑1 (MQ=255)
                                                                                          gACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCAACTCCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGAc                                                         >  2:2398320/1‑150 (MQ=255)
                                                                                           aCCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCATGAcc                                                        <  2:2355305/150‑1 (MQ=255)
                                                                                                 gCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCACCAAAGACGCGCGTGACCACTAca                                                  >  2:698964/1‑149 (MQ=255)
                                                                                                    tGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACgaga                                               >  2:904278/1‑150 (MQ=255)
                                                                                                    tGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACgaga                                               <  2:2677590/150‑1 (MQ=255)
                                                                                                    tGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACgaga                                               >  1:2776574/1‑150 (MQ=255)
                                                                                                    ccaTCCACCAATCCACCCAGGAAATCTTAGAAACCCCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTTCACCGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACgaga                                               <  1:1451364/148‑1 (MQ=255)
                                                                                                      aTCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGAtt                                             >  2:3068241/1‑150 (MQ=255)
                                                                                                       tCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTc                                            >  1:2914067/1‑150 (MQ=255)
                                                                                                       tCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTc                                            <  2:1749053/150‑1 (MQ=255)
                                                                                                       tCGAACAATCCACACAGGAAAGCTTGGAAACCGCGAAACGTACTTGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTc                                            >  2:967553/1‑150 (MQ=255)
                                                                                                          aCCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGt                                                            >  1:2839969/1‑131 (MQ=255)
                                                                                                          aCCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGt                                                            <  2:2839969/131‑1 (MQ=255)
                                                                                                          aCCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCa                                         >  2:1180917/1‑150 (MQ=255)
                                                                                                          aCCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCa                                         >  1:1634619/1‑150 (MQ=255)
                                                                                                            cAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACt                                       >  1:89212/1‑150 (MQ=255)
                                                                                                                 cACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATaa                                  >  1:1258645/1‑150 (MQ=255)
                                                                                                                  aCCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAg                                 >  1:2862427/1‑150 (MQ=255)
                                                                                                                   cccAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGc                                >  1:672714/1‑150 (MQ=255)
                                                                                                                    ccAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGtt                                                                                                     >  1:3047719/1‑80 (MQ=255)
                                                                                                                    ccAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGtt                                                                                                     <  2:3047719/80‑1 (MQ=255)
                                                                                                                    ccAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTAACCGTTCTGGTCTCCCCGCACGTCAACAAAGACACGCGTGACCAGTACGAGATTCGCAc                                        <  2:678480/141‑1 (MQ=255)
                                                                                                                    ccAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTAACCGTTCTGGTCTCCCCGCACGTCAACAAAGACACGCGTGACCAGTACGAGATTCGCAc                                        >  1:678480/1‑141 (MQ=255)
                                                                                                                     cAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCgt                              <  1:3068241/150‑1 (MQ=255)
                                                                                                                      aGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCgtg                             <  1:1180917/150‑1 (MQ=255)
                                                                                                                      aGGAAATCGTGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCgtg                             >  1:2812187/1‑150 (MQ=255)
                                                                                                                        gAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGtt                           <  2:452207/150‑1 (MQ=255)
                                                                                                                           aTCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCACAGCGAGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTg                        <  1:2674119/150‑1 (MQ=255)
                                                                                                                               tGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGc                                                                                                        <  2:2086829/66‑1 (MQ=255)
                                                                                                                               tGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGc                                                                                                        >  1:2086829/1‑66 (MQ=255)
                                                                                                                               tGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACa                    <  2:2914067/150‑1 (MQ=255)
                                                                                                                               tGGAAACCGCGAAACGTACTCGTGCACAAGTGCGTAGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAGGCGTGTTCTGGACa                    >  1:578043/1‑150 (MQ=255)
                                                                                                                                ggAAACCGCGAAACGTACTCGTGCACAAGTGCGTAGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAGGCGTGTTCTGGACAt                   <  2:578043/150‑1 (MQ=255)
                                                                                                                                       gcgAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATaa                                  >  2:2773612/1‑128 (MQ=255)
                                                                                                                                       gcgAAACGTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATaa                                  <  1:2773612/128‑1 (MQ=255)
                                                                                                                                              gTACTCGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCAGCCAACgg     <  1:1081521/150‑1 (MQ=255)
                                                                                                                                                 cTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTATTCTGGACTCCGTTTAGCTAACGGATa  <  2:2577068/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCAGCCAACGGATA  >  NC_002947/550613‑550907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A