Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F80 I0 R1	1105	125.4	6144758	94.6%	5812941	133.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,130,198	A→T	11.1%	intergenic (‑247/+57)	gcvH‑I ← / ← PP_0990	glycine cleavage system protein H/DoxX family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,130,198	0	A	T	11.1%	322.9 / 26.9	116	intergenic (‑247/+57)	gcvH‑I/PP_0990	glycine cleavage system protein H/DoxX family protein
Reads supporting (aligned to +/- strand): ref base A (50/53); new base T (8/5); total (58/58)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 120 total aligned reads displayed.

TTATTTATCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGG  >  NC_002947/1130049‑1130347

ttatttatCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTAAGCGGAAAAAGGATTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCAGTGTATCGTTTTCGCTatga                                                                                                                                                       >  2:2878680/1‑150 (MQ=255)

    ttatCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTa                                                                                                                                                   >  1:1077298/1‑150 (MQ=255)

    ttatCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTa                                                                                                                                                   <  1:1325778/150‑1 (MQ=255)

          aTAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaa                                                                                                                                             >  1:1314079/1‑150 (MQ=255)

           tAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            <  2:1314079/150‑1 (MQ=255)

           tAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaaaa                                                                                                                                            <  2:2166712/150‑1 (MQ=255)

           tAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaaaa                                                                                                                                            <  2:1755719/150‑1 (MQ=255)

            aGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAAc                                                                                                                                           >  1:994629/1‑150 (MQ=255)

            aGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAAc                                                                                                                                           <  1:2164427/150‑1 (MQ=255)

                tACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATa                                                                                                                                       <  1:183578/150‑1 (MQ=255)

                tACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTATACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATa                                                                                                                                       <  2:1048334/150‑1 (MQ=255)

                   gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTa                                                                                                                                                   <  2:1598530/135‑1 (MQ=255)

                   gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTa                                                                                                                                                   >  1:1598530/1‑135 (MQ=255)

                    tAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACAtg                                                                                                                                   >  1:1634442/1‑150 (MQ=255)

                       aTTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGATGTAGCGAAAACGATACATgtgg                                                                                                                                >  1:1797958/1‑150 (MQ=255)

                          tACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTacgac                                                                                                                                                      <  1:546929/125‑1 (MQ=255)

                          tACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTacgac                                                                                                                                                      >  2:546929/1‑125 (MQ=255)

                             ggAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTg                                                                                                                          >  1:1812110/1‑150 (MQ=255)

                             ggAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTg                                                                                                                          <  1:2126759/150‑1 (MQ=255)

                             ggAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGACGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTTCCTg                                                                                                                          <  2:2902835/150‑1 (MQ=255)

                                      gTATTTATTGTGTGGAATGATATCGTTACAGGTGCTTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACTACTTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGa                                                                                                                 <  1:2371701/150‑1 (MQ=255)

                                      gTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGa                                                                                                                 <  1:2433458/150‑1 (MQ=255)

                                      gTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGa                                                                                                                 <  1:3071974/150‑1 (MQ=255)

                                         tttattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGt                                                                                                              <  1:2936508/150‑1 (MQ=255)

                                          ttattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTg                                                                                                             <  1:2425818/150‑1 (MQ=255)

                                           tattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                                            <  1:1377285/150‑1 (MQ=255)

                                             ttGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaa                                                                                                                                              >  2:2888601/1‑114 (MQ=255)

                                             ttGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaa                                                                                                                                              <  1:2888601/114‑1 (MQ=255)

                                              tgtgtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCaa                                                                                                         <  2:3019536/150‑1 (MQ=255)

                                                tgtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATg                                                                                                       >  1:1884761/1‑150 (MQ=255)

                                                tgtgAAAGGATAGCTTTACAGGTGGCTCTGAGAGGAGAAGGGGTTGATCATGAAACCGTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATg                                                                                                       <  2:592377/150‑1 (MQ=255)

                                                 gtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGATGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATgg                                                                                                      <  2:1797958/150‑1 (MQ=255)

                                                  tgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATTTGGTGCCTGGACTAGCGATGTGCCAATGGc                                                                                                     >  2:2193408/1‑150 (MQ=255)

                                                  tgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGc                                                                                                     <  2:267669/150‑1 (MQ=255)

                                                    gAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGATCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAAt                                                                                                        <  1:680803/145‑1 (MQ=255)

                                                    gAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAAt                                                                                                        >  2:680803/1‑145 (MQ=255)

                                                        gATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCgg                                                                                               <  2:2322086/150‑1 (MQ=255)

                                                         atatCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGCGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGt                                                                                              >  2:1101399/1‑150 (MQ=255)

                                                                     gTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTc                                                                                  >  2:2289105/1‑150 (MQ=255)

                                                                        ccTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagc                                                                               >  2:886629/1‑150 (MQ=255)

                                                                        ccTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagc                                                                               >  1:1201698/1‑150 (MQ=255)

                                                                            tGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAACCGATCCATCGCTTGCCTGTTTTGGGGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAgat                                                                           <  1:2680098/150‑1 (MQ=255)

                                                                              agagGAAAAGGGTTTGAGAATCAAACCCTTGTGCACAGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAgatga                                                                         >  2:636923/1‑150 (MQ=255)

                                                                                agGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGaaaa                                                                                                                                            >  1:1356635/1‑81 (MQ=255)

                                                                                  gAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCa                                                                     >  2:1796277/1‑150 (MQ=255)

                                                                                    aaaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            <  2:2602632/77‑1 (MQ=255)

                                                                                    aaaGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGc                                                                   <  1:636923/150‑1 (MQ=255)

                                                                                      aGGGTTTGATGCTCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAgat                                                                           >  2:1393137/1‑140 (MQ=255)

                                                                                         gTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAga                                                                            <  2:1063660/136‑1 (MQ=255)

                                                                                         gTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAga                                                                            >  1:1063660/1‑136 (MQ=255)

                                                                                             gAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGAGTGGc                                                                                                                   >  2:2603787/1‑93 (MQ=255)

                                                                                             gAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGc                                                                                                                   <  1:2603787/93‑1 (MQ=255)

                                                                                               gCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                          >  2:2910564/1‑116 (MQ=255)

                                                                                               gCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                          <  1:2910564/116‑1 (MQ=255)

                                                                                                cATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTc                                                                                                 >  2:392111/1‑108 (MQ=255)

                                                                                                cATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTc                                                                                                 <  1:392111/108‑1 (MQ=255)

                                                                                                  tCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGgc                                                     >  2:74319/1‑150 (MQ=255)

                                                                                                      aCCCTTGTGCACCGACGGCGAGGAGGCTATGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            <  2:599717/59‑1 (MQ=21)

                                                                                                      aCCCTTGTGCACCGACGGCGAGGAGGCTATGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            >  1:599717/1‑59 (MQ=21)

                                                                                                        ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            <  2:42792/57‑1 (MQ=255)

                                                                                                        ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGaaaa                                                                                                                                            >  1:42792/1‑57 (MQ=255)

                                                                                                        ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCg                                               >  1:774195/1‑150 (MQ=255)

                                                                                                         cTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCgg                                              <  2:1231234/150‑1 (MQ=255)

                                                                                                         cTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCgg                                              >  2:2093514/1‑150 (MQ=255)

                                                                                                              gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAt                                                                                           >  1:1715029/1‑100 (MQ=255)

                                                                                                              gCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAt                                                                                           <  2:1715029/100‑1 (MQ=255)

                                                                                                               cACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCg                                                                                                >  2:605334/1‑94 (MQ=255)

                                                                                                               cACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCg                                                                                                <  1:605334/94‑1 (MQ=255)

                                                                                                                aCCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                                            <  1:1401898/81‑1 (MQ=255)

                                                                                                                aCCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                                            >  2:1401898/1‑81 (MQ=255)

                                                                                                                 ccGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagca                                                                              <  2:1007640/110‑1 (MQ=255)

                                                                                                                 ccGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagca                                                                              >  1:1007640/1‑110 (MQ=255)

                                                                                                                  cGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGTTCCAGCGAAAACGCTCCTGTGCCTCTTGCAACCAgg                                     >  2:2463245/1‑150 (MQ=255)

                                                                                                                    aCGGCGAGGAGGCTGTGTATCGTTTTCGCGACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGt                                                                                              >  1:2386215/1‑91 (MQ=255)

                                                                                                                          aggaggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCaa                             <  2:1634442/150‑1 (MQ=255)

                                                                                                                          aggaggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCaa                             <  1:2098868/150‑1 (MQ=255)

                                                                                                                           ggaggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGTATAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAag                            >  1:1567532/1‑150 (MQ=255)

                                                                                                                               cacatgtATCGTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATAACACTTTCAGCAGCTGATACAGCGAAAACGCGCCCGCGCCCCGGGCAACCAGGAGCAGCCAGAGcc                        >  1:823111/5‑150 (MQ=38)

                                                                                                                                  gtgtATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATgg                                                                                                      >  1:1783842/1‑69 (MQ=255)

                                                                                                                                  gtgtATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATgg                                                                                                      <  2:1783842/69‑1 (MQ=255)

                                                                                                                                   tgtATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGc                    >  1:475330/1‑150 (MQ=255)

                                                                                                                                     tATCGTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCg                                                       <  2:2512998/113‑1 (MQ=255)

                                                                                                                                     tATCGTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCg                                                       >  1:2512998/1‑113 (MQ=255)

                                                                                                                                     tATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCTGCGCCCCGGGCAGCCAGGAGCAGCAAGCGCgccgccg                  >  2:548897/1‑149 (MQ=255)

                                                                                                                                      aTCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCg                                               >  2:954323/1‑120 (MQ=255)

                                                                                                                                      aTCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCg                                               <  1:954323/120‑1 (MQ=255)

                                                                                                                                       tCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcag                <  1:2093514/150‑1 (MQ=255)

                                                                                                                                       tCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcag                <  1:1101399/150‑1 (MQ=255)

                                                                                                                                       tCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGATTACGCACTTTAAGCAGATGATCCAGCGGAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcag                >  1:690931/1‑150 (MQ=255)

                                                                                                                                         gTTTTCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggt              >  2:1347863/1‑150 (MQ=255)

                                                                                                                                           tttCGCTACGATGTAGCGAACGCGATACATGAGGTGCCGGTACTGGCGAGGCGCTAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtca            <  2:1567532/150‑1 (MQ=255)

                                                                                                                                            ttCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGAGATATGACTTTCTGAAGAAAATCCAGCGAATTGTCGCCCTCGTCTCGGTCAACCAGGAGCAGCAAGAGCCCTGCCcaggtcag           >  2:2648412/1‑150 (MQ=255)

                                                                                                                                             tCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtcagg          <  2:774195/150‑1 (MQ=255)

                                                                                                                                              cGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtcaggt         <  1:74319/150‑1 (MQ=255)

                                                                                                                                                cTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtgg       <  1:717253/150‑1 (MQ=255)

                                                                                                                                                cTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAggtgg       >  1:2314108/1‑150 (MQ=255)

                                                                                                                                                 tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGcc                                                        <  1:790663/100‑1 (MQ=255)

                                                                                                                                                 tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGcc                                                        >  2:790663/1‑100 (MQ=255)

                                                                                                                                                 tACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagcagc                               >  2:1231699/1‑125 (MQ=255)

                                                                                                                                                     acgTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGtgggtggg  >  1:1270135/1‑150 (MQ=255)

TTATTTATCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGAGCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAGGTGGGTGGG  >  NC_002947/1130049‑1130347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A