Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F91 I0 R1 918 53.4 2573728 94.2% 2424451 136.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,130,198 A→T 10.5% intergenic (‑247/+57) gcvH‑I ← / ← PP_0990 glycine cleavage system protein H/DoxX family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,130,1980AT10.5% 161.9 / 11.1 57intergenic (‑247/+57)gcvH‑I/PP_0990glycine cleavage system protein H/DoxX family protein
Reads supporting (aligned to +/- strand):  ref base A (28/23);  new base T (2/4);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TTATCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAG  >  NC_002947/1130053‑1130338
                                                                                                                                                  |                                                                                                                                            
ttatCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATAACTTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTa                                                                                                                                          <  1:656176/150‑1 (MQ=255)
        aGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGAAAAc                                                                                                                                  <  2:706333/150‑1 (MQ=255)
          aTTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGTCGTAGCGAAAACGa                                                                                                                                <  1:573920/150‑1 (MQ=255)
          aTTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGa                                                                                                                                >  2:136998/1‑150 (MQ=255)
               gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATa                                                                                                                              >  2:412959/1‑147 (MQ=255)
               gTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATa                                                                                                                              <  1:412959/147‑1 (MQ=255)
                         ggAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTg                                                                                                                 >  2:384120/1‑150 (MQ=255)
                              aGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTg                                                                                                            <  1:384120/150‑1 (MQ=255)
                                  gTATTTATTGTGTGGAATGATATCGTTACAGGGGCCTCGGGGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTGTTCGCTACGATGTAGCGAAAACGATACATGTGGTGCCGCGACTgggta                                                                                                        <  2:572870/150‑4 (MQ=255)
                                       tattGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGc                                                                                                   >  1:559708/1‑150 (MQ=255)
                                            tgtgGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATg                                                                                              >  2:103754/1‑150 (MQ=255)
                                               ggAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCg                                                                                                         <  1:102871/136‑1 (MQ=255)
                                               ggAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCg                                                                                                         >  2:102871/1‑136 (MQ=255)
                                               ggAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGcc                                                                                           >  2:945399/1‑150 (MQ=255)
                                                 aaTGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGccag                                                                                         >  1:1238326/1‑148 (MQ=255)
                                                      tatCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTc                                                                                    <  1:285084/150‑1 (MQ=255)
                                                       atCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGc                                                                                                          <  2:852615/127‑1 (MQ=255)
                                                       atCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGc                                                                                                          >  1:852615/1‑127 (MQ=255)
                                                       atCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCa                                                                                   >  1:375507/1‑150 (MQ=255)
                                                         cGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                 <  1:136998/150‑1 (MQ=255)
                                                           ttACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtctc                                                                               <  1:103754/150‑1 (MQ=255)
                                                              cAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAACACTGTAGGCGGGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACt                                                                            <  1:732400/150‑1 (MQ=255)
                                                                 gTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                 >  1:388503/1‑142 (MQ=255)
                                                                 gTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCAtc                                                                                 <  2:388503/142‑1 (MQ=255)
                                                                    ccTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagc                                                                      <  2:559708/150‑1 (MQ=255)
                                                                     ctctGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCATGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagca                                                                     >  2:310345/1‑150 (MQ=255)
                                                                        tGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAgat                                                                  <  2:1092587/150‑1 (MQ=255)
                                                                          agagGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAgatga                                                                >  1:1011023/1‑150 (MQ=255)
                                                                              gAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCa                                                            >  2:344754/1‑150 (MQ=255)
                                                                              cacaaGGGTTTGATGC‑TCAAACCCTTTTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCa                                                            >  1:108123/4‑150 (MQ=255)
                                                                                aaaGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGc                                                          <  1:344754/150‑1 (MQ=255)
                                                                                             cATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCg                                              >  2:287395/1‑150 (MQ=255)
                                                                                                   aCCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagc                                                                      <  2:107141/120‑1 (MQ=255)
                                                                                                   aCCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTcagc                                                                      >  1:107141/1‑120 (MQ=255)
                                                                                                     ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAg                                                                                                       >  2:1064942/1‑85 (MQ=255)
                                                                                                     ccTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAg                                                                                                       <  1:1064942/85‑1 (MQ=255)
                                                                                                      cTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCgg                                     <  1:620366/150‑1 (MQ=255)
                                                                                                       ttGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCggg                                    >  1:702478/1‑150 (MQ=255)
                                                                                                         gtgCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAAcgc                                                  >  1:233324/1‑134 (MQ=255)
                                                                                                         gtgCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAAcgc                                                  <  2:233324/134‑1 (MQ=255)
                                                                                                         gtgCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATACAGCGAAAACGCGCCGGCGCCTCGGGCa                                  >  1:133656/1‑150 (MQ=255)
                                                                                                              ccGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAg                             <  2:1011023/150‑1 (MQ=255)
                                                                                                                 aCGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGac                          >  2:158124/1‑149 (MQ=255)
                                                                                                                         taggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAaga                  >  2:879742/2‑150 (MQ=255)
                                                                                                                          aggCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAagag                 <  2:375507/150‑1 (MQ=255)
                                                                                                                               gtgtATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTCCGGTCATCTCACTTTCAGCAGATGTTGTAGCGAAAACGCGCCTGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTg            <  1:158124/150‑1 (MQ=255)
                                                                                                                                      gTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTg            <  2:232639/143‑1 (MQ=255)
                                                                                                                                      gTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTg            >  1:232639/1‑143 (MQ=255)
                                                                                                                                       ttttCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagca                        >  1:811635/1‑130 (MQ=255)
                                                                                                                                       ttttCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGagca                        <  2:811635/130‑1 (MQ=255)
                                                                                                                                       ttttCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGag                          >  1:580636/1‑128 (MQ=255)
                                                                                                                                       ttttCGCTACGTCGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGag                          <  2:580636/128‑1 (MQ=255)
                                                                                                                                       ttttCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtc    >  2:495295/1‑150 (MQ=255)
                                                                                                                                       ttttCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtc    >  2:543637/1‑150 (MQ=255)
                                                                                                                                       ttttCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtc    >  1:629717/1‑150 (MQ=255)
                                                                                                                                       ttttCGCTACGACGTAGCGAAAACGATACATGTGGTACCTTGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTGATCGGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCCGGAGCAGCAAGAGCCCTGCCcaggtc    >  1:103391/1‑150 (MQ=255)
                                                                                                                                         ttCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCcaggtcag  <  1:879742/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TTATCAATAGATTACGTAAATTTACGGAGAAGCCGTATTTATTGTGTGGAATGATATCGTTACAGGTGCCTCTGAGAGGAAAAGGGTTTGA‑GCATCAAACCCTTGTGCACCGACGGCGAGGAGGCTGTGTATCGTTTTCGCTACGACGTAGCGAAAACGATACATGTGGTGCCTGGACTGGCGAGGTGCCAATGGCCTTCGGTCATCTCACTTTCAGCAGATGATCCAGCGAAAACGCGCCGGCGCCTCGGGCAACCAGGAGCAGCAAGAGCCCTGCCCAGGTCAG  >  NC_002947/1130053‑1130338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A