Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F80 I0 R1	1105	125.4	6144758	94.6%	5812941	133.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,850,958	T→A	85.0%	M1K (ATG→AAG) †	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,850,958	0	T	A	85.0%	294.5 / 40.9	107	M1K (ATG→AAG) †	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand): ref base T (8/8); new base A (44/47); total (52/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 108 total aligned reads displayed.

GTTTCCTCAGACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGA  >  NC_002947/1850809‑1851101

gTTTCCTCGGACGGCGCATGTCGAGGCCATGGCGTTATGTCAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAt                                                                                                                                                 <  1:939701/150‑1 (MQ=255)

     cTCAGACGGCGTTTGCCTAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTAc                                                                                                                                            <  2:1615380/150‑1 (MQ=255)

         gACGGCGCATGTCGAGGCCTTGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGt                                                                                                                                        <  2:2023502/150‑1 (MQ=255)

             gcgcATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTgaga                                                                                                                                    <  1:1026157/150‑1 (MQ=255)

               gcATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGt                                                                                                                                  >  2:2058106/1‑150 (MQ=255)

                cATGTCGAGGCCATCGCGTTACCCGAAGCGGGGTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGTCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTg                                                                                                                                 <  1:2205329/150‑1 (MQ=255)

                   gTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTga                                                                                                                                      >  2:2516766/1‑142 (MQ=255)

                   gTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTga                                                                                                                                      <  1:2516766/142‑1 (MQ=255)

                    tCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTga                                                                                                                                      >  2:234502/1‑141 (MQ=255)

                          ccATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCgg                                                                                                                       >  1:2342330/1‑150 (MQ=255)

                             tGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCa                                                                                                                    >  1:2881269/1‑150 (MQ=255)

                              ggCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCaa                                                                                                                   <  1:2903794/150‑1 (MQ=255)

                                cGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAaca                                                                                                                 <  1:1042296/150‑1 (MQ=255)

                                 gTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAacac                                                                                                                <  2:1921720/150‑1 (MQ=255)

                                        gAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGc                                                                                                         >  2:2696625/1‑150 (MQ=255)

                                        gAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGAGCACCAGCCGGTCAACACTGACGGc                                                                                                         >  1:1163277/1‑150 (MQ=255)

                                         aaGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACGAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCa                                                                                                        >  2:2993346/1‑150 (MQ=255)

                                         aaGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCa                                                                                                        >  2:1875431/1‑150 (MQ=255)

                                               gCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAgg                                                                                                                                         <  1:2440607/111‑1 (MQ=255)

                                               gCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAgg                                                                                                                                         >  2:2440607/1‑111 (MQ=255)

                                                    cTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAAtctc                                                                                             >  1:2245613/1‑150 (MQ=255)

                                                     tGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCAc                                                                                                                              <  1:89725/116‑1 (MQ=255)

                                                     tGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCAc                                                                                                                              >  2:89725/1‑116 (MQ=255)

                                                       aCTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGc                                                                                                                                >  2:1144347/1‑112 (MQ=255)

                                                       aCTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGaa                                                                                          <  2:2769693/150‑1 (MQ=255)

                                                       aCTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCAGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGc                                                                                                                                <  1:1144347/112‑1 (MQ=255)

                                                          ggCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACg                                                                                                           >  1:2026539/1‑130 (MQ=255)

                                                          ggCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACg                                                                                                           <  2:2026539/130‑1 (MQ=255)

                                                           gCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCAcc                                                                                                                             >  1:942744/1‑111 (MQ=255)

                                                           gCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCAt                                                                                      <  1:2058106/150‑1 (MQ=255)

                                                           gCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCAt                                                                                      <  2:2245613/150‑1 (MQ=255)

                                                             cccTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGc                                                                                                         >  1:2310850/1‑129 (MQ=255)

                                                               cTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAg                                                                                                                                          >  1:2771127/1‑94 (MQ=255)

                                                               cTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGtt                                                                                  >  2:1555275/1‑150 (MQ=255)

                                                               cTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTAGTATG‑GTATGATGCATTCAAATGGAAAACCAGGAAGGTACAGGTGAGAGCGACCCAGCCGTTCAACACTGACGGCAGTATCAATCTCGAAGCATGGtt                                                                                  <  1:2993346/150‑1 (MQ=255)

                                                                  ggTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGa                                                                               <  1:1555275/150‑1 (MQ=255)

                                                                        gTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAgg                                                                                                                                         >  1:1683345/1‑86 (MQ=255)

                                                                        gTTGCCAACGAAGGGCGGCCGCACGGTGATCGCCAGCACTCTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                         <  2:1164936/150‑1 (MQ=255)

                                                                         ttGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgtg                                                                        <  2:2710564/150‑1 (MQ=255)

                                                                             cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTaa                                                                    >  1:1003638/1‑150 (MQ=255)

                                                                             cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGa                                                                                                             <  1:1423969/109‑1 (MQ=255)

                                                                             cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGa                                                                                                             >  2:1423969/1‑109 (MQ=255)

                                                                             cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                         <  1:1371026/145‑1 (MQ=255)

                                                                             cACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgt                                                                         >  2:1371026/1‑145 (MQ=255)

                                                                               cGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGc                                                                  <  1:2746768/150‑1 (MQ=255)

                                                                               cGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGc                                                                  <  2:2881269/150‑1 (MQ=255)

                                                                               cGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGc                                                                  <  1:1010418/150‑1 (MQ=255)

                                                                                ggAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCg                                                                 >  2:2919562/1‑150 (MQ=255)

                                                                                 gAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGt                                                                                                                                        >  2:905344/1‑78 (MQ=255)

                                                                                 gAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGt                                                                >  1:1176781/1‑150 (MQ=255)

                                                                                          ccGCACGGTGATCGCCAGCACACTCGTATG‑GTATTTGTCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGc                                                       >  1:2255001/1‑150 (MQ=255)

                                                                                            gCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGa                                                                               >  1:257757/1‑124 (MQ=255)

                                                                                            gCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGa                                                                               <  2:257757/124‑1 (MQ=255)

                                                                                                ctttccatgCAGCACAC‑CATACGAGTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGa                                                 <  1:739125‑M1/141‑1 (MQ=255)

                                                                                                 gTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGAt                                                <  1:1875431/150‑1 (MQ=255)

                                                                                                   ttccatgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCa                                                                                                                               <  1:267128‑M2/40‑1 (MQ=255)

                                                                                                   ttccatgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCa                                                                                                                               >  2:267128‑M2/31‑70 (MQ=255)

                                                                                                   ttccatgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCg                                                                                                                        >  2:3003591‑M2/31‑77 (MQ=255)

                                                                                                   ttccatgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCg                                                                                                                        <  1:3003591‑M2/47‑1 (MQ=255)

                                                                                                    aTCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATcgc                                             >  2:2064732/1‑150 (MQ=255)

                                                                                                     tCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTCAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATcgcg                                            >  2:1771008/1‑150 (MQ=255)

                                                                                                      cGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAg                                                                                                                           >  1:243345/1‑70 (MQ=255)

                                                                                                      cGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAg                                                                                                                           <  2:243345/70‑1 (MQ=255)

                                                                                                        tgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTc                                                                                                                     <  2:1787438‑M2/50‑1 (MQ=255)

                                                                                                        tgcagcacaccatacgagtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTc                                                                                                                     >  1:1787438‑M2/26‑75 (MQ=255)

                                                                                                        ccAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCg                                                                                                                        <  2:2394241/71‑1 (MQ=255)

                                                                                                        ccAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCg                                                                                                                        >  1:2394241/1‑71 (MQ=255)

                                                                                                          AGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAgcg                                       >  1:2696625‑M1/1‑150 (MQ=255)

                                                                                                               ACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGaa                                  <  1:912855‑M1/150‑1 (MQ=255)

                                                                                                                 tCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAaag                                >  2:2303924/1‑150 (MQ=255)

                                                                                                                   gTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAaagaa                              <  1:2919562/150‑1 (MQ=255)

                                                                                                                     aTG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGc                            >  2:2548501/1‑150 (MQ=255)

                                                                                                                     aTG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGc                            <  2:744672/150‑1 (MQ=255)

                                                                                                                     aTG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGc                            <  2:2342330/150‑1 (MQ=255)

                                                                                                                      tG‑GTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGtt                                                                                  <  2:2793474/95‑1 (MQ=255)

                                                                                                                       g‑gTGTGCTGCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCt                          >  1:1045067/1‑150 (MQ=255)

                                                                                                                         gtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCa                                                      <  2:292642‑M2/113‑1 (MQ=255)

                                                                                                                         gtgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACAAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCa                                                      >  1:292642‑M2/9‑121 (MQ=255)

                                                                                                                          tgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGc                        >  1:2439944‑M2/8‑150 (MQ=255)

                                                                                                                          tgtgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGc                        >  2:1323063‑M2/8‑150 (MQ=255)

                                                                                                                            tgctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGa                      <  1:796423‑M2/145‑1 (MQ=255)

                                                                                                                              ctgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGt                    >  1:2276340‑M2/4‑150 (MQ=255)

                                                                                                                               tgCATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCCCTGAAAGAAGCCTGCGAGtt                   >  2:68718‑M2/3‑150 (MQ=255)

                                                                                                                                 cATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTg                 >  2:1889374/1‑150 (MQ=255)

                                                                                                                                 cATGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGAACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTg                 <  1:2498630/150‑1 (MQ=255)

                                                                                                                                   tGGAAAGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGct               <  2:1176781/150‑1 (MQ=255)

                                                                                                                                      aaaGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGt                                                                <  1:1076783/98‑1 (MQ=255)

                                                                                                                                      aaaGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGt                                                                >  2:1076783/1‑98 (MQ=255)

                                                                                                                                        aGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGa          <  1:1889374/150‑1 (MQ=255)

                                                                                                                                        aGGTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGa          <  1:2548501/150‑1 (MQ=255)

                                                                                                                                         ggTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAg         <  2:1003638/150‑1 (MQ=255)

                                                                                                                                          gTAAAACAAAGAAGGTACAGGTGAGAGTGCACGAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAgg        >  1:647928/1‑150 (MQ=255)

                                                                                                                                          gTAAAACAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAgg        >  2:2306679/1‑150 (MQ=255)

                                                                                                                                              aaCAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGttt                  >  2:2815938/1‑136 (MQ=255)

                                                                                                                                              aaCAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGttt                  <  1:2815938/136‑1 (MQ=255)

                                                                                                                                              aaCAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTaga    <  1:1323063/150‑1 (MQ=255)

                                                                                                                                              aaCAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTaga    >  1:641581/1‑150 (MQ=255)

                                                                                                                                                cAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgtg                                                                        >  1:2471327/1‑80 (MQ=255)

                                                                                                                                                cAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCAtgtg                                                                        <  2:2471327/80‑1 (MQ=255)

                                                                                                                                                cAAAGAAGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAgaga  >  2:649673/1‑150 (MQ=255)

GTTTCCTCAGACGGCGCATGTCGAGGCCATGGCGTTATTCGAAGCGGGCTAGCTGACTGGCCCCTTGGTGCGGTTGCCACGGAAGGGCGGCCGCACGGTGATCGCCAGCACACTCGTATG‑GTGTGCTGCATGGAAAGGTAAAACAAAGATGGTACAGGTGAGAGTGCACCAGCCGGTCAACACTGACGGCAGTATCAATCTCGAAGCATGGTTGGACCATGTGGTAAGCGTCGATTCGGCACTGGATCGCGCAGCGCTGAAAGAAGCCTGCGAGTTTGCTCTTGAGGTAGAGA  >  NC_002947/1850809‑1851101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A