Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F83 I0 R1 969 96.3 4677704 94.6% 4425107 134.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 322,142 T→C 11.6% intergenic (+427/+31) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947322,1420TC11.6% 218.7 / 15.9 86intergenic (+427/+31)aguA/PP_0267agmatine deiminase/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (37/39);  new base C (4/6);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321999‑322291
                                                                                                                                               |                                                                                                                                                     
gCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGTCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGaa                                                                                                                                                 <  2:1146831/150‑1 (MQ=255)
gCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                                 <  1:1424135/150‑1 (MQ=255)
 cATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAag                                                                                                                                                <  2:1088039/150‑1 (MQ=255)
     aGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGc                                                                                                                                            <  2:845529/150‑1 (MQ=255)
     aGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAAGAGGc                                                                                                                                            <  2:1771269/150‑1 (MQ=255)
     aGGGTCAGGACGACCGACCCACGACGTCTGCGTGCCGGGCGATACCCCCAAGGCTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGGACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGc                                                                                                                                            <  1:2233142/150‑1 (MQ=255)
        gTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATTGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAgg                                                                                                                                         >  1:145825/1‑150 (MQ=255)
         tCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGt                                                                                                                                        >  2:1791154/1‑150 (MQ=255)
             gacgacCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAAGAGGCAGGTACAg                                                                                                                                    <  2:803942/150‑1 (MQ=255)
              acgacCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCCCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCCTCCTCGCGAAGAGGCAGGTAccga                                                                                                                                   >  2:644900/1‑147 (MQ=255)
                    gaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCTGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACtt                                                                                                                             >  1:2326143/1‑150 (MQ=255)
                    gaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACtt                                                                                                                             <  1:1860487/150‑1 (MQ=255)
                       ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGc                                                                                                                                            <  1:1630980/132‑1 (MQ=255)
                       ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGc                                                                                                                                            >  2:1630980/1‑132 (MQ=255)
                       ccACGACGTCTGCTTGCCGGGAGAAACACACAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTaaa                                                                                                                          <  2:611706/150‑1 (MQ=255)
                         acgacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCACGAAGAGGCAGGTACAGGCAACTTAAAAt                                                                                                                        <  2:46306/150‑1 (MQ=255)
                          cgacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATc                                                                                                                       <  1:1791154/150‑1 (MQ=255)
                           gacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                                      <  2:145825/150‑1 (MQ=255)
                                  gCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTg                                                                                                               >  2:1649476/1‑150 (MQ=255)
                                    ttGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTTTACGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTa                                                                                                             <  1:644900/150‑1 (MQ=255)
                                     tGCCGGGCGATACCCCCAAGGGTGGATCAGCGGAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAg                                                                                                            <  2:875121/150‑1 (MQ=255)
                                     ggCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAg                                                                                                            <  2:2218182/149‑1 (MQ=255)
                                     ccgatctcGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTCATTCTCTGACCACATACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAg                                                                                                            <  2:1834332/143‑1 (MQ=37)
                                      gCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGc                                                                                                           >  1:1650582/1‑150 (MQ=255)
                                        cGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTa                                                                                                         >  2:1555309/1‑150 (MQ=255)
                                             gATACCCCCAAGGAAATATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGcagatcgg                                                                                                    >  1:1834332/1‑143 (MQ=38)
                                              aTACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCGCCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAaga                                                                                                                                               >  2:789838/1‑106 (MQ=38)
                                              aTACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCGCCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAaga                                                                                                                                               <  1:789838/106‑1 (MQ=38)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGGGGCAGGTACAGGCAACTTAAAATCAGACACTTTAGCTACCCGTcgcca                                                                                               >  2:1819195/1‑150 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGaaa                                                                                                                  <  1:1818100/131‑1 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGaaa                                                                                                                  >  2:1818100/1‑131 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                                    <  2:1235997/129‑1 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                                    >  1:1235997/1‑129 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                               >  1:1531411/1‑150 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                                 >  2:1132166/1‑148 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTAAAGTCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                                 <  1:1132166/148‑1 (MQ=255)
                                                   cccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcaccac                                                                                              >  2:2226524/1‑150 (MQ=255)
                                                   cccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCCGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcaccac                                                                                              >  2:1694171/1‑150 (MQ=255)
                                                     cAAGTGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCCCCTGTACCTGCCTCTTCCCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGGCACCACaa                                                                                            <  1:1256990/150‑1 (MQ=255)
                                                              aTCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgcg                                                                                   >  1:468200/1‑150 (MQ=255)
                                                               tCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                                 <  2:1119513/87‑1 (MQ=255)
                                                               tCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                                 >  1:1119513/1‑87 (MQ=255)
                                                                   cGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                                 >  1:1919164/1‑131 (MQ=255)
                                                                   cGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                                 <  2:1919164/131‑1 (MQ=255)
                                                                    gAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAaga                                                                                                                                               <  1:648172/84‑1 (MQ=255)
                                                                    gAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAaga                                                                                                                                               >  2:648172/1‑84 (MQ=255)
                                                                           agaTGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTg                                                                      >  1:852262/1‑150 (MQ=255)
                                                                              tGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTCCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAt                                                                   <  1:2226524/150‑1 (MQ=255)
                                                                                 aGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTg                                                                >  1:859243/1‑150 (MQ=255)
                                                                                  ggCGCTGATGGCGGTGTGGCACTGATCGATTGCGCCTGTACCTGCCTCTTCGCGAGCATGCCcgc                                                                                                                                                    >  2:1848618/1‑65 (MQ=21)
                                                                                  ggCGCTGATGGCGGTGTGGCACTGATCGATTGCGCCTGTACCTGCCTCTTCGCGAGCATGCCcgc                                                                                                                                                    <  1:1848618/65‑1 (MQ=21)
                                                                                  ggCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            <  1:528513/121‑1 (MQ=255)
                                                                                  ggCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            >  2:528513/1‑121 (MQ=255)
                                                                                    cgcTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGACATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTgcgc                                                             <  1:2077755/150‑1 (MQ=255)
                                                                                       tGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGcgccgc                                                          >  1:1008489/1‑150 (MQ=255)
                                                                                             cGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGt                                                    <  2:1531411/150‑1 (MQ=255)
                                                                                                   ggCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGa                                              <  2:2326143/150‑1 (MQ=255)
                                                                                                           tCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGa                                              <  1:382702/142‑1 (MQ=255)
                                                                                                           tCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGa                                              >  2:382702/1‑142 (MQ=255)
                                                                                                           tCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTgtagt                                      <  1:1649476/150‑1 (MQ=255)
                                                                                                           tCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCACACTGGTCGCCGAGCTgtagt                                      <  2:1650582/150‑1 (MQ=255)
                                                                                                             gATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGtagtag                                    >  1:1472311/1‑150 (MQ=255)
                                                                                                              aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAg                                                                                                                                          >  1:516029/1‑47 (MQ=255)
                                                                                                              aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAg                                                                                                                                          <  2:516029/47‑1 (MQ=255)
                                                                                                              aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAgg                                   >  1:1808760/1‑150 (MQ=255)
                                                                                                                  cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGaaa                                                                                                                  <  1:297172/67‑1 (MQ=255)
                                                                                                                  cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGaaa                                                                                                                  >  2:297172/1‑67 (MQ=255)
                                                                                                                   aCCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGc                                                                                         <  2:1836042/91‑1 (MQ=255)
                                                                                                                   aCCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGc                                                                                         >  1:1836042/1‑91 (MQ=255)
                                                                                                                    ccTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGt                             <  2:852262/150‑1 (MQ=255)
                                                                                                                      tGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCa                           <  1:1882669/150‑1 (MQ=255)
                                                                                                                      tGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCa                           <  1:740962/150‑1 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAAc                                                                                                                 >  2:1667732/1‑59 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAAc                                                                                                                 <  1:1667732/59‑1 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGGGAATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt                      >  2:1435809/1‑150 (MQ=255)
                                                                                                                              ccTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTGCCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGtt                   <  1:1524754/150‑1 (MQ=255)
                                                                                                                               ctctTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTg                  >  2:1245234/1‑150 (MQ=255)
                                                                                                                                tctTCGCGGCCATGGTCGCGAAGAGGCAGGTACAGGCCACTTGAAAGCAGTAACTGTAGCTACGCGTGACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCACACTGGTCGCCGAGCTGGAGGCAGTCTTGGCCGCAATGTTGc                 >  2:30249/1‑150 (MQ=255)
                                                                                                                                tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGTAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGc                 >  2:1124820/1‑150 (MQ=255)
                                                                                                                                  ttCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTg                                          <  2:2241211/123‑1 (MQ=255)
                                                                                                                                  ttCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTg                                          >  1:2241211/1‑123 (MQ=255)
                                                                                                                                     gcgGGCATGCTCGCGAAGAGGCAGGTACAGGGAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAAt            >  2:1439870/1‑150 (MQ=255)
                                                                                                                                      cgGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATg           <  1:1245234/150‑1 (MQ=255)
                                                                                                                                       gggCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATAAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATgc          >  2:852593/1‑150 (MQ=255)
                                                                                                                                           aTGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTCGTCAGCAATGTTGCTCAATgccgcc      <  2:1808760/150‑1 (MQ=255)
                                                                                                                                              cTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGccgcccgc   >  1:964043/1‑150 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGa                                              >  1:1297181/1‑106 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGa                                              <  2:1297181/106‑1 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGca  >  1:216825/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321999‑322291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A