Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F91 I0 R1
|
918 |
53.4 |
2573728 |
94.2% |
2424451 |
136.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
4,951,018 |
C→A |
15.8% |
intergenic (‑123/+34) |
fliL ← / ← fliK |
flagellar protein FliL/flagellar hook‑length control protein FliK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 4,951,018 | 0 | C | A | 15.8%
| 110.5
/ 13.5
| 51 | intergenic (‑123/+34) | fliL/fliK | flagellar protein FliL/flagellar hook‑length control protein FliK |
| Reads supporting (aligned to +/- strand): ref base C (23/20); new base A (5/3); total (28/23) |
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.33e-01 |
TTGACTGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCCCGGAAA > NC_002947/4950873‑4951167
|
ttGACTGCTTCGCTCTTCGCCATGCCACTAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTATTCGAGGGCTCGccg > 1:1059497/1‑149 (MQ=255)
ttCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaagaa < 2:1001768/150‑1 (MQ=255)
ttCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaagaa > 1:420812/1‑150 (MQ=255)
gCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagcc > 2:970727/1‑150 (MQ=255)
gCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagcc > 2:931399/1‑150 (MQ=255)
tctTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagccag < 2:481491/150‑1 (MQ=255)
ccATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAgg < 1:958644/150‑1 (MQ=255)
cATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTACGGTTGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAACCAGCCCAggg > 1:78935/1‑149 (MQ=255)
aTGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGcc > 2:379868/1‑150 (MQ=255)
aataatCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGATCGCCCGCGAAGAAGCCAGCCCAGGCCAACAg > 2:162473/1‑150 (MQ=255)
ccGTCGCCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCa < 1:696479/150‑1 (MQ=255)
cgtcgtCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAg < 1:330331/150‑1 (MQ=255)
ccATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgtag < 1:506831/150‑1 (MQ=255)
tCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGTCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTaa > 2:65922/1‑150 (MQ=255)
gTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGAcc < 1:162473/150‑1 (MQ=255)
ttCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGTAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAg < 2:1023851/134‑1 (MQ=255)
ttCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGTAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAg > 1:1023851/1‑134 (MQ=255)
cAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCa < 1:377783/150‑1 (MQ=255)
aaaGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaagaa < 1:448597/106‑1 (MQ=255)
aaaGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaagaa > 2:448597/1‑106 (MQ=255)
ggAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTAGCGGGCTCGCCTGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATTGACCGCACTGt < 2:533068/150‑1 (MQ=255)
gagaATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTc > 1:1176719/1‑150 (MQ=255)
ttggcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGAAGGTTTTGGGGTGATTTCTACTTCGCGGGCTCGCCCGCGAAGAAGCCCGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAac < 1:931399/150‑1 (MQ=255)
gcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAaccac < 2:1176719/150‑1 (MQ=255)
ttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACct > 1:1241562/1‑150 (MQ=255)
ttATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGGAGTAATCGACCGCACTGTCGCCGACACCCACCTGCTGCTCCaaa > 2:165968/1‑150 (MQ=255)
cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCgg < 2:420812/150‑1 (MQ=255)
cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCGAAGAagc > 2:820528/1‑73 (MQ=25)
cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCGAAGAagc < 1:820528/73‑1 (MQ=25)
cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTAGCGGGGCCGCCAGAGAAGAAAAGGACCGAGGCCAACAGTGATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCgg > 1:797229/1‑150 (MQ=255)
ttttGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAACCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGgcgc > 2:860079/1‑150 (MQ=255)
tGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCCTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGTCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTAt < 1:374253/150‑1 (MQ=255)
gCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATc < 1:970727/150‑1 (MQ=255)
aGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGa > 2:733419/1‑150 (MQ=255)
cagcagGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACATCCACCTGCTGCTCCAAAGGCCgg > 1:1066433/1‑127 (MQ=255)
cagcagGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACATCCACCTGCTGCTCCAAAGGCCgg < 2:1066433/127‑1 (MQ=255)
gcagGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCAc > 1:357496/1‑150 (MQ=255)
agGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCGCGGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCAccc < 2:432810/150‑1 (MQ=255)
ggTTTTGCGGTGCTGGCTTCTTCGCGGGGTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCt > 2:950861/1‑150 (MQ=255)
tGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCg > 2:115067/1‑150 (MQ=255)
tGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCGCAGGCCAACAGTTATCAGGCGTAGCAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCCGGCCCTATCGACCGCTTCGGCCCCACCCTGCTCGGGCCGgcg > 2:175763/1‑150 (MQ=255)
gCTTCTTCGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACctgc > 2:1076914/1‑92 (MQ=255)
gCTTCTTCGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACctgc < 1:1076914/92‑1 (MQ=255)
cttcttCGCGCACTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgcgc < 2:1059497/150‑1 (MQ=255)
ttcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCgg < 1:710253/105‑1 (MQ=255)
ttcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCgg > 2:710253/1‑105 (MQ=255)
cttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgccgc > 1:183153/1‑150 (MQ=255)
cttcGCGGGCGAGCCCGCGAAGAAGCCAGCACAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgccgc > 1:816455/1‑150 (MQ=255)
cTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCCCGGa > 1:918808/1‑150 (MQ=255)
tCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCAcc < 2:1092605/119‑1 (MQ=255)
tCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCAcc > 1:1092605/1‑119 (MQ=255)
cGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCCCGGaaa < 1:530308/150‑1 (MQ=255)
|
TTGACTGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCCCGGAAA > NC_002947/4950873‑4951167
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
