Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F56 I1 R1	5	46.6	2293046	94.7%	2171514	133.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,964,155	Δ1 bp	coding (1248/1476 nt)	fleQ ←	transcriptional regulator FleQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,964,154	0	G	.	96.2%	222.6 / ‑0.0	53	coding (1249/1476 nt)	fleQ	transcriptional regulator FleQ
Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (28/23); total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGTGGCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATGC  >  NC_002947/4964012‑4964291

aaaCGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGa                                                                                                                                   >  1:23387/1‑150 (MQ=255)

          ccGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGTCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCAcgcg                                                                                                                         >  1:637886/1‑150 (MQ=255)

                       gATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCg                                                                                                            >  2:223590/1‑150 (MQ=255)

                       gATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCg                                                                                                            >  2:594038/1‑150 (MQ=255)

                         tACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCt                                                                                                          <  2:873187/150‑1 (MQ=255)

                                  cATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCACGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGGCACGCGCTCTTCCAGGTCGCTGCGCAGGCt                                                                                                 >  2:520717/1‑150 (MQ=255)

                                   aTCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTg                                                                                                >  1:1100422/1‑150 (MQ=255)

                                         cAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGAACGCAGCTTTCTGTGAAGTTTGGTGTGT‑GCTATTGATCGCAAAGCGGAACTGCAGGCGTCTGCGCAGGCTGTCGAcc                                                                                          >  1:594415/1‑150 (MQ=255)

                                          aGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCa                                                                                         <  1:223590/150‑1 (MQ=255)

                                                 gctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTc                                                                                  <  2:1100422/150‑1 (MQ=255)

                                                 gctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTc                                                                                  <  2:23387/150‑1 (MQ=255)

                                                     gATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTATGCAGGCTGTCGACCATCTGCTCGTCt                                                                              <  1:780813/150‑1 (MQ=255)

                                                     gATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTGCTGGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCt                                                                              >  1:809981/1‑150 (MQ=255)

                                                       tccggCGCTCCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTc                                                                            <  2:637886/147‑1 (MQ=255)

                                                       tCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATTGCCACTTTCTCTTCCAGGTCGCTTCGCAGGCTGTCGACCATCTGCTCGTCTTc                                                                            <  2:1018182/150‑1 (MQ=255)

                                                       tCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTc                                                                            <  1:673473/150‑1 (MQ=255)

                                                             ccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATc                                                                      >  2:581149/1‑150 (MQ=255)

                                                             ccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATc                                                                      >  1:729325/1‑150 (MQ=255)

                                                                            ccAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCt                                                                                                 <  2:377592/108‑1 (MQ=255)

                                                                            ccAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCt                                                                                                 >  1:377592/1‑108 (MQ=255)

                                                                                    tCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCa                                               >  1:318190/1‑150 (MQ=255)

                                                                                      cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGTTTGCTGAAGTTTGGTGTGTGACC‑TGGACCTCGACGCTCGCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGt                                             <  1:594038/150‑1 (MQ=255)

                                                                                      cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttcttc                                                   <  2:305946/144‑1 (MQ=255)

                                                                                      cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttcttc                                                   >  1:305946/1‑144 (MQ=255)

                                                                                       ttGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGtt                                            >  1:865794/1‑150 (MQ=255)

                                                                                              ccAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTc                                                                            <  2:1133422/111‑1 (MQ=255)

                                                                                              ccAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTc                                                                            >  1:1133422/1‑111 (MQ=255)

                                                                                                aGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGc                                   >  2:906309/1‑150 (MQ=255)

                                                                                                  gCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCa                                 >  2:401045/1‑150 (MQ=255)

                                                                                                    cTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCg                                                                           <  2:842422/106‑1 (MQ=255)

                                                                                                    cTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCg                                                                           >  1:842422/1‑106 (MQ=255)

                                                                                                    cTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAAt                               <  1:581149/150‑1 (MQ=255)

                                                                                                                 caTGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttc                                                      <  2:555468/114‑1 (MQ=255)

                                                                                                                 caTGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttc                                                      >  1:555468/1‑114 (MQ=255)

                                                                                                                          gTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGAc                                                                                           <  1:1141387/68‑1 (MQ=255)

                                                                                                                          gTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGAc                                                                                           >  2:1141387/1‑68 (MQ=255)

                                                                                                                          gTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATc         <  1:906309/150‑1 (MQ=255)

                                                                                                                             gCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACggg                  <  1:396127/138‑1 (MQ=255)

                                                                                                                             gCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACggg                  >  2:396127/1‑138 (MQ=255)

                                                                                                                              cTGAAGTTTGGTGTGT‑GCCATTGATCGACCCGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGCGCATGATCGCCa     >  2:318437/1‑150 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGTGGCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGccc     >  1:589663/1‑149 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTg                                                                                                <  1:490845/58‑1 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTg                                                                                                >  2:490845/1‑58 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCAt    <  2:470464/150‑1 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCAt    >  1:470464/1‑150 (MQ=255)

                                                                                                                               tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTGCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCAt    <  2:553449/150‑1 (MQ=255)

                                                                                                                                 aaGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATgc  >  1:934503/1‑150 (MQ=255)

                                                                                                                                   gTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTc                                           >  2:376155/1‑107 (MQ=255)

                                                                                                                                   gTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTc                                           <  1:376155/107‑1 (MQ=255)

                                                                                                                                    tttGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCg                                                  >  1:723964/1‑99 (MQ=255)

                                                                                                                                    tttGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCg                                                  <  2:723964/99‑1 (MQ=255)

                                                                                                                                        gtgtgt‑GCCATTGATCGCTACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCt                                                                                      <  1:1088544/59‑1 (MQ=38)

                                                                                                                                        gtgtgt‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCt                                                                                      >  2:1088544/1‑59 (MQ=255)

AAACGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGTGGCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATGC  >  NC_002947/4964012‑4964291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A