Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F83 I5 R1	8	66.5	3267118	94.3%	3080892	133.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,964,155	Δ1 bp	coding (1248/1476 nt)	fleQ ←	transcriptional regulator FleQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,964,154	0	G	.	100.0%	233.0 / NA	51	coding (1249/1476 nt)	fleQ	transcriptional regulator FleQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (28/23); total (28/23)

AATACGCAAACGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGTGGCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATGCGCTCGAC  >  NC_002947/4964005‑4964298

aaTACGCAAACGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGtgtgtg                                                                                                                                                  >  1:1305275/1‑150 (MQ=255)

       aaaCGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGa                                                                                                                                          >  2:1282566/1‑150 (MQ=255)

               aGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCAcg                                                                                                                                  >  2:530017/1‑150 (MQ=255)

                          ctaCGATACGGTTGGCATCGTGCAGTGCCTGCTGGATCAGGCGGTGCTCCCGGCTGCCCCGGTATTCCTTGAGGTCTAGGCCTTCGGGCGGCACCATGGCGTGGTTCCCGGAGTTTGGTGTGT‑GCCAGCGATCGCCACGCGCTCTTCCAg                                                                                                                       <  2:172702/148‑1 (MQ=255)

                                    gTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTgcgc                                                                                                             >  1:544895/1‑150 (MQ=255)

                                      tGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAg                                                                                                           <  2:1216925/150‑1 (MQ=255)

                                               ccAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGtgtgtgccat                                                                                                                                              >  2:163817/1‑103 (MQ=255)

                                               ccAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGtgtgtgccat                                                                                                                                              <  1:163817/107‑5 (MQ=255)

                                                  gcgcATCCTGCAACAGGCCCTGCCCCAGGCTACCCAGGTAGTCCCTGAGGTCCAGGCCTTCGGGCGGCAGCATGTCGTGGTTGCTGAAGTTTGGAGTGT‑GCCATTTATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCAt                                                                                               >  2:405508/1‑150 (MQ=255)

                                                    gcCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCt                                                                                             >  2:676358/1‑150 (MQ=255)

                                                    gcCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTCGCCACAT‑GCCATTGATCGCCCAGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCt                                                                                             >  1:1242044/1‑150 (MQ=255)

                                                      ctgctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGc                                                                                           <  1:676358/150‑1 (MQ=255)

                                                      ctgctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGc                                                                                           <  1:530017/150‑1 (MQ=255)

                                                      ctgctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGc                                                                                           <  1:322303/150‑1 (MQ=255)

                                                        gcttGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCATGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTc                                                                                         <  1:560326/150‑1 (MQ=255)

                                                        gctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGcc                                                                                                                                     <  1:1483798/106‑1 (MQ=255)

                                                        gctgGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGcc                                                                                                                                     >  2:1483798/1‑106 (MQ=255)

                                                             aTCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCtt                                                                                    <  2:886671/150‑1 (MQ=255)

                                                                 ggCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTc                                                                                >  2:737542/1‑150 (MQ=255)

                                                                 ggCCCTGCTCCAGGCTACCCAGGTAGTCCTTAAGGTCAAGGCGTTCGGGCGGCCGAATGGCGCGGTTGCTGCAGTTTGGTGTGT‑GACATTGATCGCGACGCGCACTTACAGGTCGCTGCGCAGGCTGTAGACCACCTGCTCGTCTTAGTc                                                                                >  2:257658/1‑150 (MQ=255)

                                                                   cccTGCTCCAGGCTACCCAGGTAGTCTTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCAt                                                                              <  1:650843/150‑1 (MQ=255)

                                                                   cccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAg                                                                                                                       <  2:1123428/109‑1 (MQ=255)

                                                                   cccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAg                                                                                                                       >  1:1123428/1‑109 (MQ=255)

                                                                   cccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGc                                                                                           <  1:659034/137‑1 (MQ=255)

                                                                   cccTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGc                                                                                           >  2:659034/1‑137 (MQ=255)

                                                                      tGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGa                                                                           <  2:1305275/150‑1 (MQ=255)

                                                                           cAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAg                                                                      >  2:1286373/1‑150 (MQ=255)

                                                                           cAGGCTACCCAGGTAGTCCTTGAGGTACAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAAGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAg                                                                      >  2:57660/1‑150 (MQ=255)

                                                                                   ccAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAAttt                                                              >  1:1480663/1‑150 (MQ=255)

                                                                                    cAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttc                                                             >  2:783887/1‑150 (MQ=255)

                                                                                       gTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttcttc                                                          >  1:1089742/1‑150 (MQ=255)

                                                                                         aGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCgg                                                        <  1:1286373/150‑1 (MQ=255)

                                                                                         aGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCgg                                                        <  2:1480663/150‑1 (MQ=255)

                                                                                             cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTc                                                                                                     <  1:1407016/101‑1 (MQ=255)

                                                                                             cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTc                                                                                                     >  2:1407016/1‑101 (MQ=255)

                                                                                             cTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGt                                                    >  2:690903/1‑150 (MQ=255)

                                                                                                    tCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTTCGCAGGCTGTCGACCATCTGc                                                                                           <  2:903688/104‑1 (MQ=255)

                                                                                                    tCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTTCGCAGGCTGTCGACCATCTGc                                                                                           >  1:903688/1‑104 (MQ=255)

                                                                                                    tCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGGATTTCTTCGGCAGTTCCGaca                                             >  1:733280/1‑150 (MQ=255)

                                                                                                      cAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGt                                                                                       >  1:776070/1‑106 (MQ=255)

                                                                                                      cAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGt                                                                                       <  2:776070/106‑1 (MQ=255)

                                                                                                      cAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGCCAGTTCCGacac                                            >  2:1108239/1‑149 (MQ=255)

                                                                                                      cAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTGTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGacac                                            <  1:1108239/149‑1 (MQ=255)

                                                                                                              cGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGGATTTCTTCGGCAGTTCCGACACGCCAATGAc                                   <  2:733280/150‑1 (MQ=255)

                                                                                                              cGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGAc                                   <  2:544895/150‑1 (MQ=255)

                                                                                                                       gcaTGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGTCATTCTGCTCGTCTTCGTCACCTCCTCGACGAAATTGGGTCGGCAGTTCCGACACGCCAATGACCCCGTACgg                          <  1:690903/150‑1 (MQ=255)

                                                                                                                       gcaTGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACgg                          <  1:315961/150‑1 (MQ=255)

                                                                                                                       gcaTGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACgg                          >  2:1358737/1‑150 (MQ=255)

                                                                                                                          tGGCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTg                       >  1:1598910/1‑150 (MQ=255)

                                                                                                                           ggCGTGGTTGCTGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGc                      >  1:617303/1‑150 (MQ=255)

                                                                                                                                      tGAAGTTTGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCCTCGACATAGCGGAATTTCTTCGGCAGTTACGACACGCCAATGACCCCGTATGGGGTCATGATCGCCAt           >  2:802135/1‑150 (MQ=255)

                                                                                                                                           tttGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGACTttcttc                                                          >  1:772004/1‑98 (MQ=255)

                                                                                                                                           tttGGTGTGT‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATttcttc                                                          <  2:772004/98‑1 (MQ=255)

                                                                                                                                               gtgtgt‑GCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATGCGCTCGAc  >  1:1206949/1‑150 (MQ=255)

AATACGCAAACGTTCAGCCGCGCGCGCAACGATACCGTTGGCATCGTCCAGCGCCTGCTGGATCAGGCCCTGCTCCAGGCTACCCAGGTAGTCCTTGAGGTCCAGGCCTTCGGGCGGCAGCATGGCGTGGTTGCTGAAGTTTGGTGTGTGGCCATTGATCGCCACGCGCTCTTCCAGGTCGCTGCGCAGGCTGTCGACCATCTGCTCGTCTTCGTCATCGACATAGCGGAATTTCTTCGGCAGTTCCGACACGCCAATGACCCCGTACGGGTGCATGATCGCCATGCGCTCGAC  >  NC_002947/4964005‑4964298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A