Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F80 I5 R1
|
8 |
61.1 |
2932718 |
95.1% |
2789014 |
135.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947 |
1,648,905 |
(A)6→7 |
intergenic (‑142/+154) |
oprB‑II ← / ← PP_1446 |
carbohydrate‑selective porin/TonB‑dependent receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 1,648,899 | 1 | . | A | 100.0%
| 116.0
/ NA
| 36 | intergenic (‑136/+160) | oprB‑II/PP_1446 | carbohydrate‑selective porin/TonB‑dependent receptor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (19/17); total (19/17) |
AGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑AAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTA > NC_002947/1648753‑1649035
|
aGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaa > 2:407855/1‑150 (MQ=255)
ttGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaaaa > 2:910897/1‑150 (MQ=255)
ggAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGATGATATAGGGGAAGTTATAAG‑aaaaaaa < 1:848513/150‑2 (MQ=255)
ggAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑aaaaaaa > 1:872929/1‑149 (MQ=255)
aTAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGACGGTTCGATGAAGCGTTTCAGCTTGAGGCGAAGAGCATATGTGTGAAGTTATAAGAAAAAAAGACAAATTGt > 1:1422113/1‑150 (MQ=255)
aGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATGTGGCGACAAGATTGTTCGATGAAGCGTTTCAACTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCg < 2:712103/150‑1 (MQ=255)
tCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGa < 2:1300373/150‑1 (MQ=255)
attattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgttgt < 1:451915/150‑1 (MQ=255)
ttattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgttgtt < 1:407855/150‑1 (MQ=255)
attGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACACGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAAAGGCCGCAGAGGATTGTTGTTGa > 1:448408/1‑150 (MQ=255)
attGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGa > 2:655874/1‑150 (MQ=255)
aaGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACCGATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAgtcgt > 1:386147/1‑150 (MQ=255)
aTCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGAGATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTaa > 1:510535/1‑150 (MQ=255)
aTCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGAGGCGGGGACGTTAGAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTaa < 1:910897/150‑1 (MQ=255)
gAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAtt > 1:219505/1‑145 (MQ=255)
gAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAtt < 2:219505/145‑1 (MQ=255)
gcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGACGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGgtg > 1:1275207/1‑150 (MQ=255)
cccatgaaTGGATATGGCTACAAGATTGTTCGATGAAGCGTTTAAGCTTGCGGCAGGGAGGATATAGGGGAACTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGgtgt < 2:510535/144‑1 (MQ=255)
cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAgg > 2:37098/1‑150 (MQ=255)
tGGCGACAAGAATGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAAGTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCAc < 2:505531/150‑1 (MQ=255)
ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAgg < 1:167683/128‑1 (MQ=255)
ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAgg > 2:167683/1‑128 (MQ=255)
cAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGa > 1:81400/1‑150 (MQ=255)
gATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgtt > 2:320411/1‑80 (MQ=255)
gATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGAttgtt < 1:320411/80‑1 (MQ=255)
gCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTa > 1:878939/1‑150 (MQ=255)
cGTTTAAGCTAGCGGCGGGAAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTAc < 2:81400/150‑1 (MQ=255)
aGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCgg > 2:1376900/1‑114 (MQ=255)
aGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCgg < 1:1376900/114‑1 (MQ=255)
gCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTa > 1:1401358/1‑150 (MQ=255)
gcggGGAGGATATAGGGGAAGTTATAAAAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCgg < 2:44771/150‑1 (MQ=255)
gaggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGaa < 2:448408/150‑1 (MQ=255)
ggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg < 2:1148792/100‑1 (MQ=255)
ggaTATAGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCggg > 1:1148792/1‑100 (MQ=255)
tataGGGGAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGt > 2:328143/1‑150 (MQ=255)
ggggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCt < 1:190915/150‑1 (MQ=255)
gggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTa > 2:486080/1‑150 (MQ=255)
aggAAGTTATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTa > 2:1003102/2‑150 (MQ=255)
ttATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc < 2:1241070/119‑1 (MQ=255)
ttATAAGAAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc > 1:1241070/1‑119 (MQ=255)
|
AGTTGGAACATGAGATAGCGCTATCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAG‑AAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTA > NC_002947/1648753‑1649035
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
