Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F60 I0 R1	73	395.3	8837978	95.5%	8440268	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,428	T→C	7.5%	K82E (AAA→GAA)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,428	0	T	C	7.5%	1023.9 / 44.1	368	K82E (AAA→GAA)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (171/166); new base C (12/17); total (184/184)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

TCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAT  >  USA300TCH1516_ALE/1744289‑1744568

 cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                                                                                            <  1:1285556/141‑1 (MQ=255)

 cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                                                                                            <  2:813476/141‑1 (MQ=255)

  aaTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                                                                                           >  1:3836178/1‑141 (MQ=255)

  aaTCGTACAACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTAACCTTGTTTAATTGCATCTTTGTGCATTGTTAAAATATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                                                                                           <  2:3024807/141‑1 (MQ=255)

   aTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCct                                                                                                                                          >  1:1746971/1‑141 (MQ=255)

    tCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCctt                                                                                                                                         <  2:3533484/141‑1 (MQ=255)

        accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCCCGAGGTAATTTCCCTTCttt                                                                                                                                     >  2:1779264/1‑141 (MQ=255)

         ccaccaTTAGCTAATAAATCATGTGTAATGAACACACGTTGACCTGGTTGAATTGCATCTTTGTACATTGTTAAAACAGTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                                                                                                    <  2:2537639/141‑1 (MQ=255)

         ccaccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCtttt                                                                                                                                    <  1:2664275/141‑1 (MQ=255)

          caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTc                                                                                                                                   <  1:71024/141‑1 (MQ=255)

           accaTTGGCTAATAAATCATCTGTAATTAACATACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCt                                                                                                                                  <  2:3415250/141‑1 (MQ=255)

           accaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCt                                                                                                                                  >  1:1409084/1‑141 (MQ=255)

            ccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTa                                                                                                                                 <  2:446760/141‑1 (MQ=255)

                  gCTAATAAATCATCTGTAATTAACACACGATGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTGCTTTTCTAACAGGt                                                                                                                           >  2:1388269/1‑141 (MQ=255)

                   cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTg                                                                                                                          <  2:1328903/141‑1 (MQ=255)

                     aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCa                                                                                                                        >  2:3189456/1‑141 (MQ=255)

                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                                                                                                     <  2:3490514/141‑1 (MQ=255)

                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                                                                                                     <  2:1450894/141‑1 (MQ=255)

                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                                                                                                     <  1:4048928/141‑1 (MQ=255)

                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATGGGATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                                                                                                     <  1:4152804/141‑1 (MQ=255)

                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                                  >  1:2015866/1‑141 (MQ=255)

                             atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAt                                                                                                                >  2:1581241/1‑141 (MQ=255)

                              tcTGTAATTAACACACGTTGACCTGGTGTAATTGCATCTTTGTGCATTGTTAAAACATTTGTGCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTACCAGCTGCCCAGCCatgc                                                                                                               >  2:1932684/1‑138 (MQ=255)

                               cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAtgc                                                                                                              >  1:1169488/1‑139 (MQ=255)

                               cTGTAATTAACACACGTTGACCTGGTTAAGAGGTGGGTTGGTGCATTGTTTCATTGTTTTTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATcc                                                                                                              <  1:1674114/141‑1 (MQ=255)

                                   aaTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACCGTTGCAAAGCCAATCCCCAt                                                                                                          >  1:100574/1‑141 (MQ=255)

                                     ttAACACACGTTGACCTGGTTTAATTGAATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCAAGAGGTAATTTCCCTTCTTTTTTACAAGGTGCAAACTCAATCCCCCttt                                                                                                        >  2:2770662/1‑140 (MQ=255)

                                       aaCACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                                                                                      >  1:55662/1‑141 (MQ=255)

                                         cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGCATa                                                                                                    >  2:2841443/1‑141 (MQ=255)

                                         cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATa                                                                                                    >  1:2697228/1‑141 (MQ=255)

                                           cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAg                                                                                                  <  2:3976048/141‑1 (MQ=255)

                                           cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAg                                                                                                  >  1:3109495/1‑141 (MQ=255)

                                            acGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAACGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGc                                                                                                 <  1:2212553/141‑1 (MQ=255)

                                             cGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATACGCt                                                                                                >  1:2261284/1‑141 (MQ=255)

                                              gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATTTTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAAGTGCAAAGCCAATCCCCATTGATTAAGCTa                                                                                               >  2:2192524/1‑141 (MQ=255)

                                              gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTa                                                                                               >  1:3335625/1‑141 (MQ=255)

                                               ttGACCTTGTTTAATTGCATCTTGTTGCATTGTTAAAACATTTGTGCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                                                                                              <  2:3110889/141‑1 (MQ=255)

                                               ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTAc                                                                                              <  2:524321/141‑1 (MQ=255)

                                               ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAACGCCAATCCCCATTGAATAAGCTAc                                                                                              <  1:2925855/141‑1 (MQ=255)

                                                 gACCTGTTTTAATTTCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATACCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg                                                                                            <  2:3270469/141‑1 (MQ=255)

                                                  aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAgg                                                                                           <  2:1029961/141‑1 (MQ=255)

                                                   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGa                                                                                          >  1:3647290/1‑141 (MQ=255)

                                                   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGa                                                                                          >  1:474914/1‑141 (MQ=255)

                                                     tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCGTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACa                                                                                        <  2:4045111/141‑1 (MQ=255)

                                                      ggTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTCCCATATTCTAGCTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTCCAAAGCCAATCCCCATTGAATAAGCTACCGGACAg                                                                                       >  2:1677989/1‑141 (MQ=255)

                                                           aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt                                                                                  <  1:345154/141‑1 (MQ=255)

                                                           aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCCTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt                                                                                  <  1:3040491/141‑1 (MQ=255)

                                                            aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATg                                                                                 >  1:1808322/1‑141 (MQ=255)

                                                             ttGCATCTTTGTGTATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGCATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa                                                                                <  2:2441777/141‑1 (MQ=255)

                                                             ttGCATCTTTGTGCATTGTTGACACATGGTTACCAGATTCGAGGTCATACTCATAACGAATGACTTCACGAGGTAATTGTCTTGCTTTTCTAACAGGTGCAAAGCCAGTCCCCATTGAATAAGTTCCAGGTCAGCCAATGa                                                                                >  1:2121141/1‑141 (MQ=255)

                                                             ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa                                                                                <  1:3998010/141‑1 (MQ=255)

                                                             ttGCATCTTTGTGAATTGCTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAGTCTCCATTTAATAAGCTGCAGGCCACCCCAtac                                                                                >  2:1838073/1‑139 (MQ=255)

                                                              tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGAt                                                                               >  1:517625/1‑141 (MQ=255)

                                                              tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGAt                                                                               >  1:4261897/1‑141 (MQ=255)

                                                              tGCATCTTTGTGCATTGTTAAAACATTTGTACCATATATTTCAACATGGGCAGTACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGAt                                                                               <  2:4271048/141‑1 (MQ=255)

                                                               gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATa                                                                              >  1:934303/1‑141 (MQ=255)

                                                               gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATa                                                                              >  1:4188025/1‑141 (MQ=255)

                                                                      tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAc                                                                       <  2:1081702/141‑1 (MQ=255)

                                                                       gtgCATTGTTAAAACATTTGTACCATGTTCTAGGTCATACTCATAACGAATGACTTAACGTGGTAATTCCCCTTCTTTTCTAACAGGTGCCCAGCCAATCTCCATTGAATAATCTACAGCACACTCAAAGCCAAAGCCAca                                                                      >  2:689374/1‑140 (MQ=255)

                                                                       gtgCATTATTAGAATATGTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcg                                                                      <  2:2633265/141‑1 (MQ=255)

                                                                          cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGATAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                   <  1:3258078/141‑1 (MQ=255)

                                                                          cATCGTTAAAACAGTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                   <  1:1932684/141‑1 (MQ=255)

                                                                                 aaaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt                                                            >  2:387904/1‑141 (MQ=255)

                                                                                  aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACATCCAATGATAAAGCCACGCGCTTCAGTTc                                                           >  1:3092985/1‑141 (MQ=255)

                                                                                  aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAggcc                                                           >  2:2299733/1‑139 (MQ=255)

                                                                                  aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                           <  1:374485/141‑1 (MQ=255)

                                                                                   aataTTTCTACCATATTCTAGGTCATACTCATAACGAATGACTGCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                          <  2:2396510/138‑1 (MQ=255)

                                                                                        ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTACTTTCCCTGCTTGTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                     >  1:3954975/1‑141 (MQ=255)

                                                                                          gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                   >  2:3680344/1‑141 (MQ=255)

                                                                                          gTACCATAGTCTAGGTGATACTCGTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                   <  2:5723/141‑1 (MQ=255)

                                                                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa                                                  >  1:2578488/1‑141 (MQ=255)

                                                                                            aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                 >  1:4408125/1‑141 (MQ=255)

                                                                                            aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                 <  2:3994657/141‑1 (MQ=255)

                                                                                            aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                 >  1:2039042/1‑141 (MQ=255)

                                                                                             ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                                >  1:2399107/1‑141 (MQ=255)

                                                                                             ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                                >  2:768597/1‑141 (MQ=255)

                                                                                             acataggcTAGGTCATACTCAGAACGAATGACTTCACGAGGTAAGTCCCCTTCTTTTCGAACAGGTGCAAAGCCAATCCCCAGTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                                <  2:1562766/134‑1 (MQ=255)

                                                                                              cATATTCTCGGTCATACTCATAACGAGTGACTTCACGAGGTAATTTCCCTTCTTTTCTCACAGGTGCAAAGCCAATCCCCATTGACTAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGatat                                               <  2:10005/141‑1 (MQ=255)

                                                                                               atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc                                              <  2:3730885/141‑1 (MQ=255)

                                                                                                  ttCTATGTCATACTCATAACGAATGACTTCACTAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                           <  2:2061747/141‑1 (MQ=255)

                                                                                                   tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCACGTCCTCCAACGATCTCAACa                                          >  2:4378331/1‑141 (MQ=255)

                                                                                                           aTACTCATAACGAATGACTTCACGCGGTCCGTTCCCACCCCCACTAAAAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                  <  1:612069/141‑1 (MQ=255)

                                                                                                            tACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCt                                 <  1:2495569/141‑1 (MQ=255)

                                                                                                              cTCATAACGAGTGACTTCACCAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                               <  2:4166162/141‑1 (MQ=255)

                                                                                                                  tAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCg                           <  1:3680344/141‑1 (MQ=255)

                                                                                                                    aCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAATCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGTa                         >  2:2017060/1‑141 (MQ=255)

                                                                                                                        aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                     >  2:3094429/1‑141 (MQ=255)

                                                                                                                           aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAACGACCCTACCCATTGGATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                  <  2:934303/141‑1 (MQ=255)

                                                                                                                           aCTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                  >  1:2331787/1‑141 (MQ=255)

                                                                                                                            cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCTCTTCAGGTCCTACAACGCTATCATCATCTCTTTCTTTTGCGTGTCCTAccc                 >  1:801855/1‑139 (MQ=255)

                                                                                                                            cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                 <  2:3965177/141‑1 (MQ=255)

                                                                                                                            cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                 >  1:1140494/1‑141 (MQ=255)

                                                                                                                            cTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                 >  1:2408521/1‑141 (MQ=255)

                                                                                                                              tCACGAGGTAATTTCCCTTCGTTTCGAACCGCTGTTAAGCCAATCCTCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtt               <  2:3795044/141‑1 (MQ=255)

                                                                                                                                acgacgGAATTTCCCTTCTTTTCTATCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACTCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtttt             <  2:1156041/141‑1 (MQ=255)

                                                                                                                                aagtggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtttt             <  1:2738720/137‑1 (MQ=255)

                                                                                                                                aCGAGTGAATTCGCCTTCTTCTCTAACAGGTGAAAAGCAAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCTGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtttt             <  2:4227176/141‑1 (MQ=255)

                                                                                                                                   aGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTACAATTTAATc          >  1:1347959/1‑141 (MQ=255)

                                                                                                                                     gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg        <  1:3577822/141‑1 (MQ=255)

                                                                                                                                         tttCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGc    <  1:1866169/141‑1 (MQ=255)

TCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAT  >  USA300TCH1516_ALE/1744289‑1744568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A