Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F71 I0 R1	28	65.2	1510494	95.1%	1436479	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,428	T→C	27.0%	K82E (AAA→GAA)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,428	0	T	C	27.0%	80.9 / 29.7	61	K82E (AAA→GAA)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (19/24); new base C (10/6); total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGT  >  USA300TCH1516_ALE/1744290‑1744563

cAATCGTACCACTAGTAGCTAATAAATCAGCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTATAAGTCATACTCATAACGAATGACTTCACGAGGTAATTccc                                                                                                                                       <  1:31845/141‑1 (MQ=255)

   tCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCctt                                                                                                                                    >  1:227393/1‑141 (MQ=255)

       accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCttt                                                                                                                                >  2:530766/1‑141 (MQ=255)

        ccaccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt                                                                                                                               <  1:662718/141‑1 (MQ=255)

           ccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATACCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCtt                                                                                                                            >  2:606921/1‑140 (MQ=255)

              gcaGCGAATAACTCATCTGTAATTAACACACGTTGACCTCGTTTAATTGCAGCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATATCGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACa                                                                                                                         <  1:222771/139‑1 (MQ=255)

              gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACa                                                                                                                         <  1:631618/141‑1 (MQ=255)

              gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACa                                                                                                                         <  1:541676/141‑1 (MQ=255)

                  cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTCCCCTTCTTTTCTACCAGGTg                                                                                                                     >  1:79375/1‑141 (MQ=255)

                  cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTg                                                                                                                     >  1:78207/1‑141 (MQ=255)

                  cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTATTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTGTAACAtgtg                                                                                                                     >  1:198307/1‑141 (MQ=255)

                   taataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATATCCCATATTTTCTAACAGGTGc                                                                                                                    >  1:469452/1‑141 (MQ=255)

                    aataaATCATCTGTAATTAACACACGTTGACCGGGTTTAATTGCATCTTTGTGAATTGTTAAAAAATTTTTACCATATTCACAGTTCTACTCCTAACCAATCACTTACCGAGGTGATTTCCCTTATTTTCTAACAgatata                                                                                                                   >  2:286419/1‑136 (MQ=255)

                       aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAg                                                                                                                <  2:502218/141‑1 (MQ=255)

                       aaaTCAACTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTACAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACATACCGAGATCTCCTGTCATCTTTTCTAACAGTTGCAAAg                                                                                                                <  2:460569/141‑1 (MQ=255)

                        agtgagttGTAATTGAGACTCGTTGAGCTGGTTTGAGTGCGTCTTTGTGGATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAGTTCCCTTCTTTTCTAACAGTTGCAAAGc                                                                                                               <  2:635022/134‑1 (MQ=255)

                        aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACGGCAGGAGGAAATTGCCCTTCGTTTCTAACAGGTGCAAAGc                                                                                                               <  1:146579/141‑1 (MQ=255)

                          tgatatgtgATTAACATACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                             <  2:448664/136‑1 (MQ=255)

                          tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                             >  1:537626/1‑141 (MQ=255)

                          tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                             <  1:549467/141‑1 (MQ=255)

                                 tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCa                                                                                                      <  1:320163/141‑1 (MQ=255)

                                   aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTATTTTCTAACATCTTAAAACCCAACCCCCCtt                                                                                                    >  2:666771/1‑141 (MQ=255)

                                    ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGAATTGTTAAAACATTTGTACTATATTCTAGGTCATACTCATAACGATTGACTTCACGAGGTACTTTCCCTTCTTTTCTATCAGGTGCAACGCCAATCCCCATTg                                                                                                   >  1:275911/1‑141 (MQ=255)

                                    ttAACACACCTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTg                                                                                                   <  1:217061/141‑1 (MQ=255)

                                      aaCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                                                                                 <  1:497243/141‑1 (MQ=255)

                                       acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCACCTTCTTTTCTAACAGGTGAAAAGCCACTCGCCATTGAAt                                                                                                >  2:182962/1‑141 (MQ=255)

                                          cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCCAGTTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAg                                                                                             <  1:248362/141‑1 (MQ=255)

                                           acGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGc                                                                                            >  1:443723/1‑141 (MQ=255)

                                                gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACACAAAAAAACCTACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAg                                                                                       >  1:355938/1‑141 (MQ=255)

                                                  ccTGGTTTAATTGTAGCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAATCTACAGGa                                                                                     <  2:673641/141‑1 (MQ=255)

                                                  ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTACCCTTCTTTTCTAACAGCTGCAAAGCCAATGCCCATTGAATAAGCTACAgca                                                                                     >  2:134300/1‑139 (MQ=255)

                                                       tttAATTGCTTCTTTGTGTATTGGTAAAACATTTGTACCATAGTCTAGGTCATACTCATAACGAATGGCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAACCAATCCCCATTGAATAAGCTACAGGACAgac                                                                                <  2:666377/141‑3 (MQ=255)

                                                        ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa                                                                               <  1:162320/141‑1 (MQ=255)

                                                          aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt                                                                             <  2:597897/141‑1 (MQ=255)

                                                           aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGTCTTCACTAGGTAATTTGCGCGATGATCGATCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATg                                                                            >  1:144218/1‑141 (MQ=255)

                                                           aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATCAGCTACAGGACAGCCCATg                                                                            >  1:545678/1‑141 (MQ=255)

                                                                aTCTTTGTGCATTGCTAACCCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGAtcat                                                                       >  2:421497/1‑138 (MQ=255)

                                                                   tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATCAGCTACAGGACAGCCAATGATAATGcc                                                                    >  2:687827/1‑141 (MQ=255)

                                                                    ttGTGCATTGTTAAAACATTTGTACCATATTCTATGGCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCa                                                                   <  2:493211/141‑1 (MQ=255)

                                                                     tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCATTCCCCATTTAATAAGCTACAGGAAAACCAATGATACGGCCAc                                                                  >  1:627960/1‑141 (MQ=255)

                                                                         cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                              <  1:61721/141‑1 (MQ=255)

                                                                           ttGTTAAAACATTTGTACCATATTCTAGGTCATAATCATAACCAATGACTGCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCtt                                                            <  1:320812/141‑1 (MQ=255)

                                                                                 aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                      <  2:227393/141‑1 (MQ=255)

                                                                                  aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGATGCAAAGCCAATCCCCATTGAATAATCTACAGGACAGCCAATGATAAACCCACGCGCTTCAGGTcc                                                     >  2:252241/1‑141 (MQ=255)

                                                                                      tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCTACGCGCTTCAGGTCCTACa                                                 >  1:420021/1‑141 (MQ=255)

                                                                                      tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa                                                 >  1:156048/1‑141 (MQ=255)

                                                                                       ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                >  1:266175/1‑141 (MQ=255)

                                                                                           aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCATGTCCTGCAACgct                                            >  1:382174/1‑139 (MQ=255)

                                                                                            ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                           >  1:268035/1‑141 (MQ=255)

                                                                                                    tAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTACCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTAAATAAACTCCGAAGGAGCCACTGATAATACCAAGCGCTTCAGGTCCTACAACAATATCAACAtc                                   >  2:189626/1‑141 (MQ=255)

                                                                                                         cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCTACAACGATATCAACATCTCTGt                              <  2:616547/141‑1 (MQ=255)

                                                                                                         cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCAAATGATAATGCCACGCCCTACAGGTCCTACAACGATATCAACATCTATGt                              >  2:469634/1‑141 (MQ=255)

                                                                                                          aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                             <  2:570024/141‑1 (MQ=255)

                                                                                                             cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                          <  2:754334/141‑1 (MQ=255)

                                                                                                             cTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCTACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                          <  2:394945/141‑1 (MQ=255)

                                                                                                             cTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGCACAGGCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                          <  1:289975/141‑1 (MQ=255)

                                                                                                             cTCATAACGAATGACTTCACGAGGGAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                          <  2:556265/141‑1 (MQ=255)

                                                                                                                   aCGTATGACTTCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTa                    <  2:248906/141‑1 (MQ=255)

                                                                                                                    cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                   <  1:5244/141‑1 (MQ=255)

                                                                                                                        tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGACAAACCCCGTTGAATCACACGCATGACATCCAATGATAAAGCCACGCGATTCAGGTCCTACACCGATATCAACATCTCTGTCTTTTCCGTATTCTa               >  1:178677/1‑141 (MQ=255)

                                                                                                                             tCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtt          <  1:682751/141‑1 (MQ=255)

                                                                                                                                     tcaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACCATTTTATCTGt  >  2:548952/3‑141 (MQ=255)

CAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGT  >  USA300TCH1516_ALE/1744290‑1744563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A