Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F60 I0 R1	73	395.3	8837978	95.5%	8440268	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,018,582	T→A	6.9%	K113* (AAA→TAA)	mgt ←	Monofunctional glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,018,582	0	T	A	6.9%	1061.1 / 42.5	348	K113* (AAA→TAA)	mgt	Monofunctional glycosyltransferase
Reads supporting (aligned to +/- strand): ref base T (165/155); new base A (14/11); total (179/169)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

ACAAATAATTCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTG  >  USA300TCH1516_ALE/2018443‑2018722

acacATAATTCTCTTACTTTTCTAGTAAATGAACTATCATTATCATAAAAATAATTTTTGACAACTTGGTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTc                                                                                                                                             <  2:2869345/141‑1 (MQ=255)

aCAAATAATTCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCATTGAAAATAAAGCTCTAGTTGTACCTTTc                                                                                                                                             >  2:1771049/1‑141 (MQ=255)

 cAAATAAATAATTTGCTGTTCTACTAGATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCa                                                                                                                                            <  1:1000543/141‑1 (MQ=255)

 aaaaTAATTCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCa                                                                                                                                            <  2:330049/140‑1 (MQ=255)

 aaaaTAATTATTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCa                                                                                                                                            <  2:2292657/140‑1 (MQ=255)

      aatTCTTTTACTTTTCTAGTAAATGAACGATCATTCTCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCg                                                                                                                                       <  2:2166213/141‑1 (MQ=255)

        ttCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGaa                                                                                                                                     >  1:3937844/1‑141 (MQ=255)

         tCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAAt                                                                                                                                    <  1:4320849/141‑1 (MQ=255)

         tCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAAt                                                                                                                                    >  1:2212250/1‑141 (MQ=255)

         tCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAAt                                                                                                                                    >  1:3593090/1‑141 (MQ=255)

          cTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATc                                                                                                                                   >  1:3076056/1‑141 (MQ=255)

            tttATTTTTCTAGTAAATGAACGATCAATATCATAAAAATAATTTTTGACAACTTGTTGGGTAATGGTACTACCACTTTGCACATCTCTGTCGCTAATCGGTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATcta                                                                                                                                 <  2:4339499/141‑3 (MQ=255)

             ttACTGTTCTAGTATATGACCGGGCTGTATCATAGAAATAATTTTTTACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAAGCGAATCCat                                                                                                                                <  2:2649149/141‑1 (MQ=255)

               aCTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCatga                                                                                                                              <  2:3517895/141‑1 (MQ=255)

               aCTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCatga                                                                                                                              >  2:848887/1‑141 (MQ=255)

               aCTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCatga                                                                                                                              <  1:1463100/141‑1 (MQ=255)

               aCTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCatga                                                                                                                              >  1:992530/1‑141 (MQ=255)

                 ttttttAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCatgatg                                                                                                                            <  2:679164/141‑1 (MQ=255)

                         tAAATGAACGATCATTATCATAACAATAATTTTTGACAACTTGTTGTTTCATGGTACTACCACCTTGCACATCTCTGCCGCTAATCGTTGCCAATAAACCTCTAGTTCTACCTCTCAAACCGCCTCCATAACGAttccacc                                                                                                                    >  2:1662917/1‑136 (MQ=255)

                         tAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGa                                                                                                                    <  1:1712716/141‑1 (MQ=255)

                         tAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATACATGATGATTGTAGa                                                                                                                    >  1:1100468/1‑141 (MQ=255)

                          aaaTGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGaa                                                                                                                   <  2:694517/141‑1 (MQ=255)

                            aTGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATc                                                                                                                 <  2:94955/141‑1 (MQ=255)

                             tGAACGCTCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCg                                                                                                                <  1:1140658/141‑1 (MQ=255)

                              gAACGATCATTATCATAAAAATAGTTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGt                                                                                                               >  2:1434928/1‑141 (MQ=255)

                              gAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGAATAATCTTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGt                                                                                                               <  1:4155780/141‑1 (MQ=255)

                               aaCGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGtt                                                                                                              >  1:1752160/1‑141 (MQ=255)

                                  gATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCAt                                                                                                           >  2:4219663/1‑141 (MQ=255)

                                  gATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTAATACCACTTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCAt                                                                                                           >  1:2810121/1‑141 (MQ=255)

                                    tCATTATCATAAAAATAATTTTTGATAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCt                                                                                                         <  1:3498163/141‑1 (MQ=255)

                                      aTTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTc                                                                                                       <  1:3345951/141‑1 (MQ=255)

                                       ttATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTcc                                                                                                      >  1:2290468/1‑141 (MQ=255)

                                       ttATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTcc                                                                                                      >  2:2328860/1‑141 (MQ=255)

                                         atgagaaaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCGCCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCAt                                                                                                    <  2:573857/136‑1 (MQ=255)

                                         aTCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGCAAATAAAGCTCTAGTGGTGCTGGTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCAt                                                                                                    <  1:3031048/141‑1 (MQ=255)

                                         aTCATAAAAATAATTTTATACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCAt                                                                                                    <  2:1326341/141‑1 (MQ=255)

                                          tCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCAtt                                                                                                   <  1:378513/141‑1 (MQ=255)

                                           cATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGGCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTg                                                                                                  <  1:4358310/141‑1 (MQ=255)

                                              aaaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTTGAAGAACACGCTATATTGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGaaa                                                                                               <  1:3452863/141‑1 (MQ=255)

                                                 aataatTTTTGACAACTTGTTGTGTAATGATACTACCAACTTGCACATCTCTGTCGCTTATCGTTGAAAATAAAGATCTAGTTGTAGCTTTCAAATCGAATCTATAATGATTGTAGAAACGTACACCTTCATTTGAAATaa                                                                                            >  2:895204/1‑141 (MQ=255)

                                                     atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTTAAATAAAGGc                                                                                        >  2:1848285/1‑141 (MQ=255)

                                                     atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGc                                                                                        <  1:15457/141‑1 (MQ=255)

                                                     atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGc                                                                                        <  2:2563602/141‑1 (MQ=255)

                                                       ttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCAc                                                                                      <  1:3572089/141‑1 (MQ=255)

                                                       ttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGAAc                                                                                      >  2:447132/1‑141 (MQ=255)

                                                        tttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTCCCTTTCAAATCGAATCCATGATGATTATAGAATCGTTCATCTTCCATTGAAATaaattcact                                                                                     >  2:509615/1‑135 (MQ=255)

                                                         ttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCt                                                                                    >  1:2668926/1‑141 (MQ=255)

                                                          tGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCtt                                                                                   >  1:1243027/1‑141 (MQ=255)

                                                            aCAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                 >  1:4076378/1‑141 (MQ=255)

                                                                   gttttGTAATTGTTCGACCTCCGTGCACATCTCTGTCGCTAATCTTTGAAAATAAAGCTCTATTTGTACCTTTCAAATCGATTCCATGCTCATTGCAGAATCGTTCATCTTCCATTGATATAAAGGCACCTTTCACATAct                                                                          <  2:1398580/141‑1 (MQ=255)

                                                                   gttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATAct                                                                          <  1:2867425/141‑1 (MQ=255)

                                                                     tgtgTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATActct                                                                        >  1:918892/1‑141 (MQ=255)

                                                                      gtgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTg                                                                       <  2:4128433/141‑1 (MQ=255)

                                                                       tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTgg                                                                      >  1:3236974/1‑141 (MQ=255)

                                                                            tGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGt                                                                 >  2:1055289/1‑141 (MQ=255)

                                                                                  tacCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGGTTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAg                                                           >  2:2435865/1‑141 (MQ=255)

                                                                                    ccaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCt                                                         <  1:688944/141‑1 (MQ=255)

                                                                                    ccaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCt                                                         <  2:2223661/141‑1 (MQ=255)

                                                                                     caccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTCGAACCGATCAGCTTTCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCCGCTg                                                        >  1:2075140/1‑141 (MQ=255)

                                                                                     caccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTTCTCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATTTTTTctgttt                                                        >  1:53249/1‑136 (MQ=255)

                                                                                     caccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATAGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATAAGCtn                                                        >  1:2698097/1‑140 (MQ=255)

                                                                                          tGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCTAATGCATGACCATTATAGCTTCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGAcaca                                                   <  1:165413/141‑1 (MQ=255)

                                                                                          tGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATTATGATTGTAGAATCGTTCATCTTCCATTGCAATAAAGGCACCTTTAACATCCTTTGGCATCTTATCAGCTGCcaca                                                   >  2:1929359/1‑141 (MQ=255)

                                                                                           gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaa                                                  <  2:2918634/141‑1 (MQ=255)

                                                                                            cacaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATTTTATCAGCTGACACaaa                                                 >  2:2104104/1‑141 (MQ=255)

                                                                                             atgacattGTCGCTAATCGTTGAAAAGAAAGCTCTAGTTGTACCTTTCAAATCGAATGAGTGATGATTGTAGAATTGTTCATCTTCCATTGAAATAAAGGAACCTTTAACATACTCTGGCATGTTATTGCCTAACACaaaa                                                >  1:3259545/8‑141 (MQ=255)

                                                                                             acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGCTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa                                                >  2:2983995/1‑141 (MQ=255)

                                                                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCTTTCATCTTCCATCGAAATAAAGGCACCTTTAACATACCCTGGCATGTTATCAGCTGACACAAAac                                               <  1:2568399/141‑1 (MQ=255)

                                                                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                               >  2:3920486/1‑141 (MQ=255)

                                                                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTACCACAAAac                                               >  1:1965670/1‑141 (MQ=255)

                                                                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTACATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                               >  2:3209513/1‑141 (MQ=255)

                                                                                               aTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACCAAact                                              >  1:4409539/1‑141 (MQ=255)

                                                                                                tctcTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                             >  2:3593649/1‑141 (MQ=255)

                                                                                                    tGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt                                         >  2:4023424/1‑141 (MQ=255)

                                                                                                    tGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt                                         >  2:3673391/1‑141 (MQ=255)

                                                                                                    tGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt                                         >  1:1057657/1‑141 (MQ=255)

                                                                                                        gCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGACATGTTATCAGCTGACCCAAAACTACTTTTAtt                                     >  1:401759/1‑141 (MQ=255)

                                                                                                           aaTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTc                                  <  1:3830194/141‑1 (MQ=255)

                                                                                                           aaTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTc                                  <  1:4219663/141‑1 (MQ=255)

                                                                                                             tCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAACGGCTCCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCaa                                <  1:637775/141‑1 (MQ=255)

                                                                                                             tCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCaa                                >  1:481861/1‑141 (MQ=255)

                                                                                                              cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAAt                               >  1:2194612/1‑141 (MQ=255)

                                                                                                               gTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATACAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAAtt                              >  2:3378360/1‑141 (MQ=255)

                                                                                                                 tGAAAATAAAGCTCTAGTTGTACCTTTCAAGTGGAATACATGATGGTTGTAGAATCGTTCTTCTTCCATTTAAATAAAGGTACCTTTAACATACACTGGCATGTTATTAACTGTCACAACACTATTTTTATCTTCGAttat                            >  2:3836234/1‑139 (MQ=255)

                                                                                                                  gAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAAttttt                           >  1:1786665/1‑141 (MQ=255)

                                                                                                                    aaaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCt                         <  1:3694378/141‑1 (MQ=255)

                                                                                                                     aaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                        >  2:3028258/1‑141 (MQ=255)

                                                                                                                      aTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTa                       <  1:810380/141‑1 (MQ=255)

                                                                                                                        aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt                     >  1:3629844/1‑141 (MQ=255)

                                                                                                                         aagttgtAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                    <  2:204570/135‑1 (MQ=255)

                                                                                                                         aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                    >  2:2711150/1‑141 (MQ=255)

                                                                                                                         aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                    >  1:1350998/1‑141 (MQ=255)

                                                                                                                              ctAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATcc               >  1:729603/1‑141 (MQ=255)

                                                                                                                                aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcac             <  2:1243027/141‑1 (MQ=255)

                                                                                                                                  ttGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACCAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCAACAt           >  2:3768233/1‑141 (MQ=255)

                                                                                                                                  tcgTACCTCTCAAATCGAGTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCAGCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACAt           <  2:1128929/139‑1 (MQ=255)

                                                                                                                                     tACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTAt        >  2:3473160/1‑141 (MQ=255)

                                                                                                                                      aCCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTAAAAGATTGTCGTGTGTTGCTTTTATTTTCAATTTTTCTTAGTTCATCCACatcatc       >  2:3126775/1‑141 (MQ=255)

                                                                                                                                       ccTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg      <  1:2349281/141‑1 (MQ=255)

                                                                                                                                          ttCAAATCGATTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTGTcgcct   >  2:1564299/1‑139 (MQ=255)

ACAAATAATTCTTTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTG  >  USA300TCH1516_ALE/2018443‑2018722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A