Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F60 I1 R1
|
14 |
18.2 |
455004 |
92.7% |
421788 |
134.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
2,018,582 |
T→A |
K113* (AAA→TAA) |
mgt ← |
Monofunctional glycosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 2,018,582 | 0 | T | A | 100.0%
| 59.1
/ NA
| 22 | K113* (AAA→TAA) | mgt | Monofunctional glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/12); total (10/12) |
TTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCC > USA300TCH1516_ALE/2018455‑2018709
|
tttACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTATTCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCg < 1:172693/135‑1 (MQ=255)
aGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCatgatg < 1:104055/135‑1 (MQ=255)
tGAACGATCATTATAATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACAGCTCTGTCTCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTa < 2:149729/135‑1 (MQ=255)
aaCGATCATTATCATAAAAATAATTTTTGACAATTTGTTTTGTAATGGTACTACCACGTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGa < 2:197888/135‑1 (MQ=255)
aaCGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGa > 1:136299/1‑135 (MQ=255)
ataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTACACATAACAGACACTAATAGTTGAAAATAAAGATCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTACAATCGTTCATCTTCCATTGa > 1:41839/1‑135 (MQ=255)
atTTTTTACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAACAAAGCTCTAGTTGTACCTTACAAATTTATTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAAt < 1:106064/135‑1 (MQ=255)
cTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATTATTGTAGATTCGTACATCTTTCTATGAATTAAAGGcccgtt > 2:178706/1‑130 (MQ=255)
tgtgTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACa > 2:177008/1‑135 (MQ=255)
tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATa < 2:136299/135‑1 (MQ=255)
aaTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATTTTCCATTGAAATAAAGGCACCTTTAACATActc > 2:1766/1‑135 (MQ=255)
caccTTGCACATCTCTGTCGCTAATCGTTTAAAATAAAGCTCTAGTTGTACCTTACAAATCGATTTCATGATGATTGTAGAATCGTTCATTTTCTATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTAt < 1:50368/135‑1 (MQ=255)
accTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCTATGATGATTGTATAATCGTTTATTTTTCATTTAAATAAAGGCACCTTTAACATACTCTGGCATGTTATc < 1:36021/135‑1 (MQ=255)
ttGCACATCTATGTCGCTAATTGTTGAAAATAAAGCTCTAGTTGTACATTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACGTTGAACATACTTTGGCATGTTATCAGc < 2:36277/135‑1 (MQ=255)
gTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTTCATTGAAATAAAGGCTCCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAact < 1:164352/135‑1 (MQ=255)
gTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAact > 2:114153/1‑135 (MQ=255)
tAATCGTTGAAAATAAAGCTCTAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt > 1:194442/1‑135 (MQ=255)
aGCTCTAGTTGTACCTGACAAATCGATTTTCTGATGAGTGAAGAAACGTTCATCTTCCATTGAAATAAAGACACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCt > 1:202660/1‑135 (MQ=255)
atAGTTCTACCTTATAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTCATTTTCAATTTTTCTTAGt > 2:25553/2‑135 (MQ=255)
tAGTTGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAATCTACTTTTATTTTCAATTTTTCTTAGtt < 1:1766/135‑1 (MQ=255)
tGTACCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACACAAGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATc > 2:86054/1‑135 (MQ=255)
gTATCTTACAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATTAAGGCACTTTTAACTTTCTCTAGTATTTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATcc < 1:46313/135‑1 (MQ=255)
|
TTTACTTTTCTAGTAAATGAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCC > USA300TCH1516_ALE/2018455‑2018709
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
