Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F44 I1 R1
|
12 |
24.7 |
574166 |
94.6% |
543161 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
2,532,298 |
A→T |
T15T (ACA→ACT) |
femA_3 → |
Aminoacyltransferase FemA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0%
| 68.5
/ NA
| 24 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/13); total (11/13) |
GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATT > USA300TCH1516_ALE/2532164‑2532409
|
gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGATTAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg > 2:279136/1‑141 (MQ=255)
gtAATTGTAATCAGTTTTAGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 2:237347/141‑1 (MQ=255)
tAATCAGTTTTTGATCCTTTCCAAGAACATCAGTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt > 2:235244/1‑141 (MQ=255)
ttaataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac < 2:109121/138‑1 (MQ=255)
ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑ACCGTTGACTTCGGATGAGTTCACTGCGTTTACTACTAATCTATTTTCACGTTacac > 1:99832/1‑141 (MQ=255)
taaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa > 1:85112/1‑141 (MQ=255)
aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCAGAGAGGAATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTACATTACACACAATTAGTTAt > 2:92942/1‑141 (MQ=255)
ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt < 1:171145/141‑1 (MQ=255)
ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt < 1:250156/141‑1 (MQ=255)
taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGAGTTCGGATGAGTTCAATCAGTTTACTAACAAGCATTTTTCACGTGACACACAATCAGCTCTCCCTTACAAtaaaa > 2:236136/1‑137 (MQ=255)
aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGt > 2:224830/1‑141 (MQ=255)
tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATATAGTTGAt > 2:223073/1‑141 (MQ=255)
aaaaaTAATGGATGAATGCGGAGGGGGGTGGGAGAAAATGATCTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAttt < 2:99832/141‑1 (MQ=255)
aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa < 2:78073/141‑1 (MQ=255)
aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa < 1:124854/141‑1 (MQ=255)
aaTTATATTTCAATGCAGAGAGGGATGGGATAAAATGAGCGTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACCAAGCAGTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa < 2:117270/141‑1 (MQ=255)
ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc < 2:56383/141‑1 (MQ=255)
ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc < 1:107362/141‑1 (MQ=255)
ttgagagagGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCATCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt < 2:111259/138‑1 (MQ=255)
tCAGAAAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc < 1:92028/141‑1 (MQ=255)
aagagagGGATGGGATAAAATGATCTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 2:127815/140‑1 (MQ=255)
tgggatAAAATGAACTTTGTGATTGTTG‑CATCGGATGAGGTCAATGCGTTTACTACAAAGAATCAATCACAGGACACACATTCATCTATTCAGTATAAGCATATGTTGGATGTAAAAGGCGATGTTCATCTTGtcaggctt > 2:5466/1‑134 (MQ=255)
gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAGTACACACAGTCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt > 2:35397/1‑141 (MQ=255)
ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAGAGGCGATGTGCATCTTGTAGTGGTTAAAGATGACAATGGTCAAGTGAtt > 2:16621/1‑141 (MQ=255)
|
GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGT‑AACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATT > USA300TCH1516_ALE/2532164‑2532409
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
