Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F71 I0 R1 28 65.2 1510494 95.1% 1436479 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA100.0% 167.5 / ‑6.5 67A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (44/22);  minor base C (1/0);  total (45/22)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTT  >  USA300TCH1516_ALE/2279568‑2279842
                                                                                                                                           |                                                                                                                                         
ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTCGCTTGCGATTTATCATTTGCATCATCAGT‑TGCTGATGTTACtg                                                                                                                                        >  2:22262/1‑141 (MQ=255)
ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACtg                                                                                                                                        >  1:629363/1‑141 (MQ=255)
 tgtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACtgt                                                                                                                                       >  1:492428/1‑141 (MQ=255)
   tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACtgttg                                                                                                                                     >  2:423599/1‑141 (MQ=255)
    gCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACtgttgt                                                                                                                                    >  2:554282/1‑141 (MQ=255)
     tctttattaGTTCATTCTACTTCTGTTTTACTAGTCACTGCGTTATCACATT‑TTGTTGTTTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTgttgtt                                                                                                                                   <  2:495620/138‑1 (MQ=255)
     ccGTTATTAGTTTATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATTTGCAGT‑TGCTGATGTTACTgttgtt                                                                                                                                   <  2:237705/141‑1 (MQ=255)
        ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGATGTTTCa                                                                                                                                >  2:507628/1‑141 (MQ=255)
        ttatcaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACGGTGTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTACCATTTGCATCATCAGT‑GCCTGCTGTTACTGGTGttttc                                                                                                                                >  2:580828/1‑139 (MQ=255)
              gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                          >  1:443960/1‑141 (MQ=255)
              gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCCGATGTTCCTGTTGTTTCACCTGtt                                                                                                                          >  2:257081/1‑141 (MQ=255)
               ttGATTCTACTTCTGGTTTGCTAGTTACATCGTGATT‑CATTGTCGGACTGTTTGTAGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                         <  2:42574/141‑1 (MQ=255)
               ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                         >  1:690118/1‑141 (MQ=255)
               gtGATTCTGCTTCTTGTCTACAAGTTACATTGTGTTC‑TATTGTCGGACTGTTTGTTTATGCATCTACACTAGGATTGTTATTAGTTTGCGGTTTATCATGTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                         <  2:99091/140‑1 (MQ=255)
                tGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                        >  1:49695/1‑141 (MQ=255)
                tGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                        <  1:490392/141‑1 (MQ=255)
                tGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                        <  2:658994/141‑1 (MQ=255)
                   ttCTACTTCTGGTTTACTAGTTACATCTTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTTATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                     >  2:78647/1‑141 (MQ=255)
                     cTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAt                                                                                                                   <  1:602749/141‑1 (MQ=255)
                       aCTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATTCATCTACTCTAGAATTGTTATTCGTCTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCGCCATCa                                                                                                                 >  2:374398/1‑141 (MQ=255)
                        cTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                                >  2:571943/1‑141 (MQ=255)
                          tCTGGTTTACTAGTTACATCGTTATC‑CATTGACGGTCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCCTTTGCATCATCAGT‑TGCTGGTGTTACTGTTGTTTCACCTGTTCCCCCATCACtt                                                                                                              >  2:249434/1‑140 (MQ=255)
                             ggTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatta                                                                                                           >  1:613391/1‑141 (MQ=255)
                             ggTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGAATGATGATGGATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCCTTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCTtattt                                                                                                           >  2:234266/1‑140 (MQ=255)
                                     aGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt                                                                                                   >  1:307406/1‑141 (MQ=255)
                                            tCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga                                                                                            >  1:507825/1‑141 (MQ=255)
                                               ttATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa                                                                                         >  1:488277/1‑141 (MQ=255)
                                                tATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAACTTGCGATGTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAg                                                                                        >  2:570728/1‑141 (MQ=255)
                                                 aTC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc                                                                                       <  2:747973/141‑1 (MQ=255)
                                                  tC‑CATTGTTGGATTGTTTGGTGATGCATCTATACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTTATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                      <  2:735136/141‑1 (MQ=255)
                                                   c‑cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                                     >  1:145696/1‑141 (MQ=255)
                                                     cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTCTTGCCGCATCACTATTATTTGGTGTTTTCCGTGAATCGTc                                                                                    >  2:108134/1‑141 (MQ=255)
                                                      aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGCGAAGCGTCt                                                                                   >  2:725366/1‑141 (MQ=255)
                                                        tGTCGGTCTGTTTGTTGTTGTATCTACATTAGAATTGTTATTAGCTTGTGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTTCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGc                                                                                 <  2:501526/141‑1 (MQ=255)
                                                               tggtttgttGATGCTTCTACACTAGAATTGTTAGTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                          <  2:397441/139‑1 (MQ=255)
                                                                 gtttgttGATGCATCTACACTAGAATTGTTATTAGCTTTCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                        <  2:263560/141‑1 (MQ=255)
                                                                        gATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtc                                                                 >  2:93244/1‑141 (MQ=255)
                                                                              tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                           <  1:602956/141‑1 (MQ=255)
                                                                               cTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg                                                          >  1:707982/1‑141 (MQ=255)
                                                                                      aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                   >  1:700661/1‑141 (MQ=255)
                                                                                      aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTCCTTTGCTATTAGCTGTCGTCTCACGTACGtt                                                   >  2:257979/1‑141 (MQ=255)
                                                                                      aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTGGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATCCGtt                                                   >  2:194060/1‑141 (MQ=255)
                                                                                          ttGTTATTAGCTTGCGGTTTATCATTTGCATCGTCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGt                                               <  2:690118/141‑1 (MQ=255)
                                                                                          ttGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGACGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTATGCCATTAGCTGTCGTCTCAGATACGTTAGGt                                               <  2:666650/141‑1 (MQ=255)
                                                                                          ttGTTATTAGCTTGCGGTTTATCATTTGCATCAACAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATATGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGt                                               >  2:161641/1‑141 (MQ=255)
                                                                                            gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                             >  2:618155/1‑141 (MQ=255)
                                                                                            gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg                                             >  2:590355/1‑141 (MQ=255)
                                                                                              tcttaGCTTGCAGTTGATCATGTGTATCAT‑AGTGTGCTGATGTTACTGTTTTTTTTCCTTTTGGCGTATTTCTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCTTTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                           <  2:224362/139‑1 (MQ=255)
                                                                                                ttaGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTAGGATAATCTCCTGCTTTGCCATTAGCTGTCGTCTCAGCTACGTTAGCTTGTCCa                                         >  2:160527/1‑141 (MQ=255)
                                                                                                ttaGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCa                                         >  1:494524/1‑141 (MQ=255)
                                                                                                      tGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                   >  1:257457/1‑141 (MQ=255)
                                                                                                      tGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGGCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                   <  1:571987/141‑1 (MQ=255)
                                                                                                            ttATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                             <  1:1982/141‑1 (MQ=255)
                                                                                                            ttATCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTAGTATCTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTTTCGTCTCAGATACTTTAGCTTGTCCAGTACTTTCCGgt                             >  2:298436/1‑141 (MQ=255)
                                                                                                              aTCATTTGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTGCAGTCTTTTCTGgtgt                           >  2:317976/1‑141 (MQ=255)
                                                                                                                   ttGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCAt                      >  2:207349/1‑141 (MQ=255)
                                                                                                                    tGCATCATCAGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAtt                     >  2:109242/1‑141 (MQ=255)
                                                                                                                        tcatcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa                 >  1:430420/1‑141 (MQ=255)
                                                                                                                        tcatcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCa                 <  1:78647/141‑1 (MQ=255)
                                                                                                                          atcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGCTACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAGTAGCAtt               >  2:617337/1‑141 (MQ=255)
                                                                                                                          atcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt               >  1:126997/1‑141 (MQ=255)
                                                                                                                           tcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt              >  1:270090/1‑141 (MQ=255)
                                                                                                                           tcaGT‑TGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt              >  1:607742/1‑141 (MQ=255)
                                                                                                                               t‑tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTACTTGGTGTTGTCGGCGAAGCCTCTCCTTCGCCCTTCGCTGTCCTCCCAGATACGTTAGGCTGTCCAGTCTTTTCTGCTGATGCATTACAATTTGAAt          >  2:308516/1‑141 (MQ=255)
                                                                                                                                 tgcgggtGTTACTGTTGGTTCGCGTGTTGCCGCATCACGATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt         <  2:449583/135‑1 (MQ=255)
                                                                                                                                       tGTTACTGTTGTTTCACCTGTTGCCGTATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt   <  1:264478/141‑1 (MQ=255)
                                                                                                                                        gTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGtt  <  1:219477/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATC‑CATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGT‑TGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGTT  >  USA300TCH1516_ALE/2279568‑2279842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A