Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F58 I0 R1 35 61.1 1410250 95.0% 1339737 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA100.0% 168.1 / ‑6.5 65A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/31);  minor base T (0/2);  total (32/33)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841
                                                                                                                                         |                                                                                                                                         
ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt                                                                                                                                         >  1:82671/1‑140 (MQ=255)
   tgCCGTTACTAGTTGATTCGACTTCTTGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTGTCATTTGCATCATCAGTTGCTGATGTTACtgtt                                                                                                                                      <  2:449318/140‑1 (MQ=255)
       gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                  >  1:613721/1‑140 (MQ=255)
        ttattaGTTGATTCTACTTCTGGTTTACTAGTGACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATGTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                                 <  2:566841/140‑1 (MQ=255)
            tctgtGACTCTATCTCTGCTTTCCTAGTTTCATCCTTCTCCATTGTCGCCGTGCTTGTTGATGCATCTACACTAGAACTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt                                                                                                                             <  1:53506/136‑1 (MQ=255)
            taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt                                                                                                                             <  1:453678/140‑1 (MQ=255)
              gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt                                                                                                                           >  1:621895/1‑140 (MQ=255)
               ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                          >  2:617420/1‑140 (MQ=255)
               ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                          >  1:562411/1‑140 (MQ=255)
                tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTAGTGTTGTTTCACATGTTg                                                                                                                         >  1:475369/1‑140 (MQ=255)
                tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGATGAGGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                         >  2:465243/1‑140 (MQ=255)
                 gATTCTACTTTTGGTTTACTAGTTACCTTGTTATCTATTGTCGGACTGTTTGTTGATGCATCTGCACTAGAATTGTTATTAGCTTCCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                        <  2:139027/140‑1 (MQ=255)
                 gATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc                                                                                                                        <  1:672377/140‑1 (MQ=255)
                 gATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCCGTTGc                                                                                                                        >  1:58668/1‑140 (MQ=255)
                   ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg                                                                                                                      <  1:289229/140‑1 (MQ=255)
                      tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAt                                                                                                                   <  1:569727/140‑1 (MQ=255)
                           cTGGTTTACTAGTGACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta                                                                                                              <  2:590200/140‑1 (MQ=255)
                                 ttctggTTATATCGTTTTCCATTTTCGGTCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt                                                                                                        <  2:190995/135‑1 (MQ=255)
                                  aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                       >  1:122794/1‑140 (MQ=255)
                                       ttACCTCTTTATCCATTCGCGGACTGTTTGTTCATCCATCTACACTAGCATTGTTATTAGCTTGCGGTTTATCTTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgt‑t                                                                                                 <  1:390839/140‑1 (MQ=255)
                                            tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCgg                                                                                            <  1:381993/140‑1 (MQ=255)
                                             cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGga                                                                                           >  2:115336/1‑140 (MQ=255)
                                               ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTTACCTGTTGCCGCATTACTATTGTTTGGTGT‑TGTCGgaga                                                                                         >  2:534612/1‑140 (MQ=255)
                                                   ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGCTTATCATTTGCATCATCAGTTGCTGATGTTCCTGTTGTTTCACCTGTTTCTGCTTCTCTCTTATCTCGTGT‑TGTCTGAGaaaac                                                                                     >  1:133751/1‑137 (MQ=255)
                                                      ttGTCGGACTGTTTGTTGATGCATCTAGAATAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCCGTTGCCGAATCACTATTGTCTGGTGTGTGT‑GGAGAACCGTCt                                                                                  >  2:218532/1‑140 (MQ=255)
                                                      ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCt                                                                                  >  1:50418/1‑140 (MQ=255)
                                                        gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGAGT‑TGTCGGAGAGGGGGCTGc                                                                                >  1:251056/1‑140 (MQ=255)
                                                          ctgccTGTTTGTTGATGCATCTACACTAGATTTGTTGTTACCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTTTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCtt                                                                              <  2:66094/136‑1 (MQ=255)
                                                          ctcattgtttgttGCTGAATCTGTCCTAGACTCTTTATTAGGTTGCTGTTTATCTTTTGCATCGTCAGTTTTTGATGTTACTGTTGTTTCCCCTGTTTCCGCCTCACTATTATTTTGTGT‑TGTCGGAGAAGCGTCTGCtt                                                                              <  2:379407/135‑1 (MQ=255)
                                                                      tgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTGTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTAGGGGAAGAGTCTGCGTTGGCATTAGcgg                                                                  >  1:665073/1‑138 (MQ=255)
                                                                             tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                           >  1:536534/1‑140 (MQ=255)
                                                                                acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        >  2:402394/1‑140 (MQ=255)
                                                                                acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  1:427828/140‑1 (MQ=255)
                                                                                     aGAATTTTTATTCGCTTGCGGTTTATCATGTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGGCGTCTCAGATACGt                                                   <  2:695625/140‑1 (MQ=255)
                                                                                     aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                   >  1:176580/1‑140 (MQ=255)
                                                                                        atggTTATTTGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAg                                                <  1:199498/137‑1 (MQ=255)
                                                                                           gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTAACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTCGCTGTCGTCTCAGATACGTTAGGtt                                             >  2:9598/1‑140 (MQ=255)
                                                                                              attaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGCAGCGTCTGCTTTGCCATTCGCGGTCGTCTCAGATACGCTAggtggtg                                          >  2:262813/1‑139 (MQ=255)
                                                                                                taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACGTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCa                                        <  1:59726/140‑1 (MQ=255)
                                                                                                 tgctttgggTTTCTCCTTTTCATCCTCTGTTGCTTAGGTTACTGTTGTTTCACCTGTTGGCGCATCACTATTATTTGGTTG‑TGTTGGAGAAGCGTCTGCTTTGCTATTTTCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                       <  2:103545/133‑1 (MQ=255)
                                                                                                 aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                       <  1:115336/140‑1 (MQ=255)
                                                                                                     tGCGGTTTATCAGTTGCATCATCAGGTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt                                   <  1:305201/140‑1 (MQ=255)
                                                                                                      gCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                  >  1:582717/1‑140 (MQ=255)
                                                                                                      gCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAGCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                  <  1:254614/140‑1 (MQ=255)
                                                                                                       cGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                                 >  1:201842/1‑140 (MQ=255)
                                                                                                       cGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCCGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                                 <  2:58668/140‑1 (MQ=255)
                                                                                                       cGGTTTATCATGTGCATCATCAGTTGCTGATGTTACGGTTGTTTCACCTGTTGCCGCATCACGATTATTTGGTGT‑TGTCGGAGCACGGCCGGCGTTGCCATTACCCTGCGTCCCAGATACGTAAGGTTGTGCAggacgat                                 >  2:461136/1‑134 (MQ=255)
                                                                                                        ggTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCGGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGATGTCGGCTCGGATACGTTACGGTGTCGAGTATTTTc                                >  2:632724/1‑140 (MQ=255)
                                                                                                         gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               <  1:121626/140‑1 (MQ=255)
                                                                                                            tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:151360/140‑1 (MQ=255)
                                                                                                              tcttttGCTTCTTCAGTTTGTGTTCTTTCGGTGGTTTCCCCTGTTGCCGCATCACTTTTATTTTGTTT‑TGTCGGAGAAGCGTCTGCTTTGCCATTTGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                          <  2:643262/137‑1 (MQ=255)
                                                                                                                 tttGCATCGTCGGTTGCTGATGTTTCTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                       <  2:106888/140‑1 (MQ=255)
                                                                                                                 tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc                       >  1:460193/1‑140 (MQ=255)
                                                                                                                     catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                   >  1:590915/1‑140 (MQ=255)
                                                                                                                     catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATGAATAGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                   >  1:630903/1‑140 (MQ=255)
                                                                                                                       tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGc                 <  1:617420/140‑1 (MQ=255)
                                                                                                                             gTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa           <  1:634639/140‑1 (MQ=255)
                                                                                                                                gttGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGTTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        <  2:125126/138‑1 (MQ=255)
                                                                                                                                gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        >  1:670729/1‑140 (MQ=255)
                                                                                                                                gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        >  1:60794/1‑140 (MQ=255)
                                                                                                                                gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        >  2:103543/1‑140 (MQ=255)
                                                                                                                                  tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTg      <  1:516102/140‑1 (MQ=255)
                                                                                                                                    aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTGTTTTCTGGTGTTGGATTAGCATTTGAATTTGCt    >  2:298176/1‑140 (MQ=255)
                                                                                                                                    aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt    <  2:621895/140‑1 (MQ=255)
                                                                                                                                      gTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt  <  2:43344/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGT‑TGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A