Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F2 I1 R1
|
13 |
29.1 |
961540 |
98.8% |
950001 |
141.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,429,936 |
G→T |
H101Q (CAC→CAA) |
purF ← |
amidophosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,429,936 | 0 | G | T | 100.0%
| 102.0
/ NA
| 30 | H101Q (CAC→CAA) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/16); total (14/16) |
CCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTGTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAA > NC_000913/2429794‑2430073
|
ccAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGc > 7:47195/1‑148 (MQ=255)
aGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAg > 1:57059/1‑149 (MQ=255)
gCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGATTATCTTCAAAAAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCa > 4:52519/1‑148 (MQ=255)
ggAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATAc > 8:62358/1‑149 (MQ=255)
gTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACgggg > 7:84616/1‑149 (MQ=255)
gTCCAGCGCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGGTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAg < 3:1119/148‑1 (MQ=255)
cAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTa < 2:80555/148‑1 (MQ=255)
gCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACgg > 3:98488/1‑149 (MQ=255)
gAAGATATTAAGCAGAATTTCCGAGTCGGATGTGGGGTTGATGTGGCGGCGTTTTTCTTCAACCAGTTGTTTACGCCACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCACGCGTAATGCCCGACGGGGAGGGAACGTAAAACGGCt > 7:57638/1‑149 (MQ=255)
gAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCt > 6:70963/1‑149 (MQ=255)
aaGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTg > 2:60990/1‑149 (MQ=255)
atatTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTgcgc < 8:84616/149‑1 (MQ=255)
gCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAAAGTAAAACGGCTGCGCTTCagag > 5:98786/1‑149 (MQ=255)
cGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAg < 5:116339/148‑1 (MQ=255)
cGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGc > 2:109358/1‑149 (MQ=255)
gAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCt < 4:98488/149‑1 (MQ=255)
gAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGc > 7:73401/1‑149 (MQ=255)
tggtgTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTg < 5:70963/149‑1 (MQ=255)
gtgtTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTggg < 4:630/149‑1 (MQ=255)
ggcgTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACAtaa < 7:18281/149‑3 (MQ=255)
tttCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAAt < 8:47195/148‑1 (MQ=255)
aaCAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACgg > 5:67701/1‑86 (MQ=255)
aaCAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACgg < 6:67701/86‑1 (MQ=255)
aGTTTTTTACGCAACTCTGGCGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGcc < 8:57638/149‑1 (MQ=255)
aGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGcc < 1:60990/149‑1 (MQ=255)
tttACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGc < 2:57059/148‑1 (MQ=255)
aaCTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTg < 8:73401/149‑1 (MQ=255)
tCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCa > 4:104249/1‑148 (MQ=255)
tCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCa < 6:104368/149‑1 (MQ=255)
aGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCaaa < 7:62358/149‑1 (MQ=255)
|
CCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTGTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAA > NC_000913/2429794‑2430073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTGTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAAT > NC_000913/2429793‑2430074
|
TCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCA > NB501016:228:HFK2FAFXY:4:11608:10930:11410/1‑150 (MQ=60)
CAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGC > NB501016:228:HFK2FAFXY:1:11305:5914:8040/1‑151 (MQ=60)
AGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGT > NB501016:228:HFK2FAFXY:4:21509:12333:13420/1‑151 (MQ=60)
TGTCCAGCGCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGGTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGT < NB501016:228:HFK2FAFXY:2:11101:7915:15400/150‑1 (MQ=60)
GGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGC > NB501016:228:HFK2FAFXY:2:21306:15224:1432/1‑151 (MQ=60)
CGAAGATATTAAGCAGAATTTCCGAGTCGGATGTGGGGTTGATGTGGCGGCGTTTTTCTTCAACCAGTTGTTTACGCCACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCACGCGTAATGCCCGACGGGGAGGGAACGTAAAACGGCTG > NB501016:228:HFK2FAFXY:4:21403:1851:19116/1‑151 (MQ=60)
AGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAAAGTAAAACGGCTGCGCTTCAGAGG > NB501016:228:HFK2FAFXY:3:21510:1204:14822/1‑151 (MQ=60)
CCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGC < NB501016:228:HFK2FAFXY:3:21608:13463:15380/150‑1 (MQ=60)
GGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCC > NB501016:228:HFK2FAFXY:4:21501:9146:8661/1‑151 (MQ=60)
GTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGG < NB501016:228:HFK2FAFXY:3:21405:14162:14388/151‑1 (MQ=60)
CGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATAAC < NB501016:228:HFK2FAFXY:4:11412:2549:6178/151‑1 (MQ=60)
AAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGC > NB501016:228:HFK2FAFXY:3:21403:1347:13328/1‑88 (MQ=60)
CAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCC < NB501016:228:HFK2FAFXY:1:11307:17412:8842/151‑1 (MQ=60)
GAGATTGCCGTTTTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAAT < NB501016:228:HFK2FAFXY:4:21406:25499:19529/151‑1 (MQ=60)
|
TCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGAAGATATTAAGCAGAATTTCCGAGTCGGAAGTGGTGTTGATGTGGCGGCGTTTTTCTTCAAACAGTTTTTTACGCAACTCGTGAGCGTTGGTCAGATTGCCGTTGTGGGCAAGCGTAATGCCATACGGGGAGTTAACGTAAAACGGCTGCGCTTCAGAGGCGCTGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAAT > NC_000913/2429793‑2430074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
