Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F47 I1 R1
|
19 |
28.1 |
1106256 |
97.8% |
1081918 |
136.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
NC_000913 |
2,720,868 |
(CGCTGG)2→3 |
coding (916/2661 nt) |
pka → |
protein lysine acetyltransferase |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
NC_000913 |
= 2720855 | 1 (0.030) | 24 (0.790)
+GCGCTG |
21/232 |
0.4 |
96.0% |
coding (903/2661 nt) |
pka |
protein lysine acetyltransferase |
| ? | NC_000913 |
2720856 = |
1 (0.030) | coding (904/2661 nt) |
pka |
protein lysine acetyltransferase |
CCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2720708‑2720855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGT > NC_000913/2720856‑2720996
||||||
CCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCG > 2:139208/1‑149
CCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGC > 1:123571/1‑149
GCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCT < 2:7304/149‑1
TGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAAT < 1:128539/149‑1
GCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCCACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATG > 2:76542/1‑149
CGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCGACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATG < 5:107034/148‑1
AGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTG > 8:36177/1‑149
AGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTG < 7:42128/149‑1
AGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTG < 1:139208/149‑1
CCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACG < 7:36177/149‑1
ACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCT > 3:100150/1‑148
ACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGC > 3:57861/1‑108
ACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGC < 4:57861/108‑1
AGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGC > 5:10983/1‑149
GGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACC > 2:69659/1‑148
GGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACC < 1:69659/148‑1
CGGTGGAAACCCTTAGCCATATGCGCCCGCCACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGC < 1:76542/149‑1
GTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCA < 6:10983/149‑1
CCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTG < 4:100150/149‑1
TTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCCGAAACTGCGC > 7:76085/1‑149
AGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGC < 2:123571/147‑1
GCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAA > 1:1376/1‑148
AGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATC < 2:1376/148‑1
CAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATAGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTAC > 8:10524/1‑149
GCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCA > 4:62866/1‑149
GCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGT > 4:60129/1‑147
GCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGT < 3:60129/147‑1
||||||
CCCGGCATGGGATGCGGCTATTCAGCGTGCCGGTTTGTTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2720708‑2720855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGT > NC_000913/2720856‑2720996
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑GCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTGAGCACTATATTAAAACGCTGG > NC_000913/2720745‑2721018
|
GTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATG < NB501016:228:HFK2FAFXY:1:21210:23082:16064/151‑1 (MQ=60)
GCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCGACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGG < NB501016:228:HFK2FAFXY:3:21506:13392:11485/150‑1 (MQ=60)
CAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGG < NB501016:228:HFK2FAFXY:4:11512:16265:15344/151‑1 (MQ=60)
CAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGG < NB501016:228:HFK2FAFXY:1:21303:22189:14033/151‑1 (MQ=60)
CCCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGC < NB501016:228:HFK2FAFXY:4:11509:12109:5644/151‑1 (MQ=60)
CACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTA > NB501016:228:HFK2FAFXY:2:21210:19344:15329/1‑150 (MQ=60)
CACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑GCGCTGGCGCTGGcgct > NB501016:228:HFK2FAFXY:2:11309:5176:16167/1‑106 (MQ=60)
GAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCG > NB501016:228:HFK2FAFXY:3:11406:10730:12839/1‑151 (MQ=60)
CGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCT < NB501016:228:HFK2FAFXY:1:11308:15021:3636/150‑1 (MQ=60)
GCGGTGGAAACCCTTAGCCATATGCGCCCGCCACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCC < NB501016:228:HFK2FAFXY:1:11311:1464:4683/151‑1 (MQ=60)
CTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCCGAAACTGCGCG > NB501016:228:HFK2FAFXY:4:21408:13016:14796/1‑151 (MQ=60)
CGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAAC > NB501016:228:HFK2FAFXY:1:11101:8966:12561/1‑150 (MQ=60)
CTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑GCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCG > NB501016:228:HFK2FAFXY:4:11504:10334:9005/1‑151 (MQ=60)
TTATCAGCAACGGTGCTGCGCCTGCCGCGCTGGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCG > NB501016:228:HFK2FAFXY:1:21104:5438:7024/1‑150 (MQ=60)
cgcgctgGCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTG < NB501016:228:HFK2FAFXY:2:11310:8897:19037/142‑1 (MQ=60)
GCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTGAGCACTATATcaaac > NB501016:228:HFK2FAFXY:1:21211:17200:8760/1‑146 (MQ=60)
GGATGCCTTATAGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTGAGCACTATATTAAAACGCT < NB501016:228:HFK2FAFXY:4:11406:23378:17926/150‑1 (MQ=60)
GATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTGAGCACTATATTAAAACGCTGG < NB501016:228:HFK2FAFXY:2:11312:20339:6703/151‑1 (MQ=60)
|
TTGCGGGTACAGGACACCCACGAGCTGTTTTCGGCGGTGGAAACCCTTAGCCATATGCGCCCGCTACGTGGCGACCGGCTGATGATTATCAGCAACGGTGCTGCGCCTGCC‑‑‑‑‑‑GCGCTGGCGCTGGATGCCTTATGGTCACGCAATGGCAAGCTGGCAACGCTAAGCGAAGAAACCTGCCAGAAACTGCGCGATGCACTGCCAGAACATGTGGCAATATCTAACCCGCTCGATCTACGCGATGACGCCAGCAGTGAGCACTATATTAAAACGCTGG > NC_000913/2720745‑2721018
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
