Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F43 I1 R1
|
23 |
34.4 |
1194698 |
96.8% |
1156467 |
139.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
NC_000913 |
2,721,224 |
(GGCTGCCG)1→2 |
coding (1272/2661 nt) |
pka → |
protein lysine acetyltransferase |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
NC_000913 |
= 2721212 | 0 (0.000) | 33 (1.020)
+GCCGGGCT |
26/236 |
0.2 |
100% |
coding (1260/2661 nt) |
pka |
protein lysine acetyltransferase |
| ? | NC_000913 |
2721213 = |
0 (0.000) | coding (1261/2661 nt) |
pka |
protein lysine acetyltransferase |
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2721068‑2721212
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCT > NC_000913/2721213‑2721358
||||||||
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCG < 2:130112/149‑1
GCCCAGCGACGCTGCTCCCGCAACAGAAAGCGCGCAAGGATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATTTGTCTCTTTGCTGACGCACTGGTGCGGCGAGCACTCCTCGCCAGAGGCACGACGTTGATTCAGCGAAGCCG > 7:55134/1‑149
CCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGG < 3:54609/149‑1
GCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCC > 2:32529/1‑149
CCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGA < 7:12398/149‑1
AGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCG > 6:98516/1‑149
CAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAA < 3:17021/149‑1
AAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAG > 5:49446/1‑149
TATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAA < 1:32529/149‑1
TAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAA < 3:18060/146‑1
TAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAA > 4:18060/1‑146
TTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCA > 5:108669/1‑149
GAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACT > 5:116626/1‑149
TAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACC < 6:43004/124‑1
TAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACC > 5:43004/1‑124
AGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGC < 6:49446/149‑1
CCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTG > 5:33870/1‑148
CCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTG > 1:71874/1‑148
CCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTG < 6:116626/148‑1
TCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAAT < 1:168996/148‑1
CTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAAT > 7:105739/1‑147
TCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAAT > 1:102000/1‑146
CTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGA < 5:98516/149‑1
TGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGA < 6:33870/146‑1
TGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAA < 8:88045/147‑1
ACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAAC < 5:25390/147‑1
CGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTAC < 8:105739/149‑1
ACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGC > 2:43895/1‑149
CTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCC < 6:108669/149‑1
TCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCC > 5:34902/1‑148
TCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGC < 2:133220/111‑1
TCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGC > 1:133220/1‑111
AAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATC < 6:34902/149‑1
ATTCCGCGAAGCCGGGCGGCCGGGCTGCCGACCCACCGTCCCCCGGAAGGAACCATCACTGCTGATATGCATATGGTGGAGTACCGGCGTAATCATAAGCAACTCCGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCG < 8:55134/148‑1
GCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCT < 2:71874/149‑1
||||||||
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2721068‑2721212
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCT > NC_000913/2721213‑2721358
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAA‑‑‑‑‑‑‑‑GCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCA > NC_000913/2721084‑2721352
|
CCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAA‑‑‑‑‑‑‑‑GCCGGGCTGCCGggctgccgac < NB501016:228:HFK2FAFXY:4:11407:2181:7088/151‑11 (MQ=60)
GCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAG < NB501016:228:HFK2FAFXY:2:11110:11226:5496/151‑1 (MQ=60)
CAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGG > NB501016:228:HFK2FAFXY:3:11511:13660:18628/1‑151 (MQ=60)
GTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAC < NB501016:228:HFK2FAFXY:1:11202:14939:5291/151‑1 (MQ=60)
TTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAC < NB501016:228:HFK2FAFXY:2:11110:12801:16329/148‑1 (MQ=60)
ATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCAC > NB501016:228:HFK2FAFXY:3:21503:18258:6150/1‑151 (MQ=60)
TGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTG > NB501016:228:HFK2FAFXY:3:21507:8377:1915/1‑151 (MQ=60)
GTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCC > NB501016:228:HFK2FAFXY:3:11508:6298:19506/1‑126 (MQ=60)
CCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGG > NB501016:228:HFK2FAFXY:3:11504:1636:15272/1‑150 (MQ=60)
CCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGG > NB501016:228:HFK2FAFXY:1:11306:20055:16143/1‑150 (MQ=60)
GTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATC < NB501016:228:HFK2FAFXY:1:21312:2926:4890/150‑1 (MQ=60)
TCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATC > NB501016:228:HFK2FAFXY:4:21509:9045:4505/1‑149 (MQ=60)
CTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATC > NB501016:228:HFK2FAFXY:1:21107:8193:8825/1‑148 (MQ=60)
GCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAA < NB501016:228:HFK2FAFXY:3:21410:18782:8176/151‑1 (MQ=60)
GACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACT < NB501016:228:HFK2FAFXY:3:11412:10987:17794/149‑1 (MQ=60)
CTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCC > NB501016:228:HFK2FAFXY:3:11505:7069:5348/1‑150 (MQ=60)
CTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCG > NB501016:228:HFK2FAFXY:1:21208:17183:16267/1‑113 (MQ=60)
CTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCA < NB501016:228:HFK2FAFXY:4:21406:25555:17926/134‑1 (MQ=60)
|
CCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAA‑‑‑‑‑‑‑‑GCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCA > NC_000913/2721084‑2721352
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
