Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,753,749 A→G I10V (ATC→GTC)  pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,753,7490AG99.4% 554.5 / ‑4.6 171I10V (ATC→GTC) pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (91/79);  total (91/80)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 174 total aligned reads displayed.

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838
                                                                                          |                                                                                         
tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTc                                                                                         >  1:2446701/1‑93 (MQ=255)
tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTc                                                                                         <  2:4103488/93‑1 (MQ=255)
  cGGCAGTCCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCgg                                                                                       <  1:4293475/93‑1 (MQ=255)
   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      >  1:121573/1‑93 (MQ=255)
      aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCg                                                                                   <  1:771244/93‑1 (MQ=255)
      aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCg                                                                                   >  1:1650871/1‑93 (MQ=255)
       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  >  2:4400980/1‑93 (MQ=255)
           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              >  2:1930320/1‑93 (MQ=255)
            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             >  2:228808/1‑93 (MQ=255)
            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             >  1:1905962/1‑93 (MQ=255)
            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             <  1:4460606/93‑1 (MQ=255)
             ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcca                                                                            >  1:4076567/1‑92 (MQ=255)
             ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCg                                                                            >  1:1651965/1‑93 (MQ=255)
              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           <  1:318216/93‑1 (MQ=255)
               ataaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:3362580/91‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:1691657/1‑93 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  2:3167991/93‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:1272216/93‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:1181603/93‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:3660210/93‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:3807564/93‑1 (MQ=255)
               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTCGCACCGTCGGACCGAAAACCGaa                                                                          >  2:2593078/1‑93 (MQ=255)
                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         <  2:2247903/93‑1 (MQ=255)
                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:1124346/93‑1 (MQ=255)
                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       >  1:2582469/1‑93 (MQ=255)
                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:1142456/93‑1 (MQ=255)
                   aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTg                                                                      >  1:751625/1‑93 (MQ=255)
                    gCAAGTTTCTCCCATCCTTCACAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGa                                                                     >  1:4501324/1‑93 (MQ=255)
                     cAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGag                                                                    >  1:1460244/1‑92 (MQ=255)
                     cAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGaa                                                                    <  2:3548391/93‑1 (MQ=255)
                      aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAag                                                                   >  1:2105150/1‑93 (MQ=255)
                       aGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAaga                                                                  >  2:1356974/1‑93 (MQ=255)
                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                >  2:806668/1‑93 (MQ=255)
                          ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAt                                                               <  2:1754542/93‑1 (MQ=255)
                          ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAt                                                               <  1:364708/93‑1 (MQ=255)
                           tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATg                                                              >  1:2667546/1‑93 (MQ=255)
                            ctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGt                                                             <  1:558037/93‑1 (MQ=255)
                             tcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGtt                                                            >  1:2422615/1‑93 (MQ=255)
                             tcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGtt                                                            >  2:1219320/1‑93 (MQ=255)
                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           <  1:1815113/93‑1 (MQ=255)
                               ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                                          >  2:3006671/1‑93 (MQ=255)
                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGTGCGAAAACCGAATCTGAAGAGATGTTAGc                                                         >  1:3235421/1‑93 (MQ=255)
                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         >  1:1547821/1‑93 (MQ=255)
                                  tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTa                                                       <  1:2000011/93‑1 (MQ=255)
                                  tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTa                                                       >  2:928364/1‑93 (MQ=255)
                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGc                                                 >  2:2062721/1‑93 (MQ=255)
                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                >  1:1279041/1‑93 (MQ=255)
                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  1:4683818/1‑93 (MQ=255)
                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             >  2:3254592/1‑93 (MQ=255)
                                              tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                                           >  2:2875003/1‑93 (MQ=255)
                                              tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                                           >  1:2356268/1‑93 (MQ=255)
                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          <  1:1171857/93‑1 (MQ=255)
                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGGGATGTTAGCTAAAATGCTGGACGCt                                         <  1:413798/93‑1 (MQ=255)
                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         >  2:2688407/1‑93 (MQ=255)
                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         >  2:2557276/1‑93 (MQ=255)
                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        >  1:4137179/1‑93 (MQ=255)
                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  1:1696196/93‑1 (MQ=255)
                                                  gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       <  2:1645442/93‑1 (MQ=255)
                                                  gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       >  1:3733758/1‑93 (MQ=255)
                                                   actaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGc                                      <  2:3776674/93‑1 (MQ=255)
                                                   actaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGc                                      >  2:317425/1‑93 (MQ=255)
                                                     taagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACAGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                    >  2:4183257/1‑93 (MQ=255)
                                                      aagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                                   >  1:2902441/1‑93 (MQ=255)
                                                       agactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                                  >  1:458446/1‑93 (MQ=255)
                                                        gactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGaa                                 >  1:4093840/1‑93 (MQ=255)
                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                >  1:3122269/1‑93 (MQ=255)
                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               >  1:4656742/1‑93 (MQ=255)
                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              <  1:378303/93‑1 (MQ=255)
                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  2:2919235/1‑93 (MQ=255)
                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  1:1067815/1‑93 (MQ=255)
                                                            ggcATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             <  2:2472488/91‑1 (MQ=255)
                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             >  2:1416682/1‑93 (MQ=255)
                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  2:3918043/93‑1 (MQ=255)
                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            >  2:4308762/1‑93 (MQ=255)
                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:2290971/93‑1 (MQ=255)
                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            >  1:1157024/1‑93 (MQ=255)
                                                              cATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                           <  1:203445/93‑1 (MQ=255)
                                                              cATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                           <  1:2968864/93‑1 (MQ=255)
                                                               aTGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                          >  1:550746/1‑93 (MQ=255)
                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:2883702/93‑1 (MQ=255)
                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      >  2:3791040/1‑93 (MQ=255)
                                                                    aaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt                     >  2:4496328/1‑93 (MQ=255)
                                                                     aaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                    <  1:1085918/93‑1 (MQ=255)
                                                                      aGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                   <  1:4514687/93‑1 (MQ=255)
                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  2:4705890/1‑93 (MQ=255)
                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAc                 <  1:1673523/93‑1 (MQ=255)
                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              <  2:881000/93‑1 (MQ=255)
                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  2:3833379/1‑93 (MQ=255)
                                                                            aaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtct             >  2:818098/1‑93 (MQ=255)
                                                                             aaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctc            <  2:417234/93‑1 (MQ=255)
                                                                             aaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctc            >  2:4405963/1‑93 (MQ=255)
                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc          >  1:959317/1‑93 (MQ=255)
                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         <  1:2372032/93‑1 (MQ=255)
                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         <  1:1131683/93‑1 (MQ=255)
                                                                                 gTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt        <  2:2430822/93‑1 (MQ=255)
                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  2:1510993/93‑1 (MQ=255)
                                                                                   ttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAATATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATgg      <  1:403642/93‑1 (MQ=255)
                                                                                   ttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATgg      <  2:1556216/93‑1 (MQ=255)
                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     <  1:1744450/93‑1 (MQ=255)
                                                                                       aCCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAc  >  2:143475/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753658‑1753842
                                                                                           |                                                                                             
TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTC                                                                                             >  SRR4302544.2470029/1‑94 (MQ=60)
 TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCG                                                                                            <  SRR4302544.4427604/94‑1 (MQ=60)
  CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           >  SRR4302544.1684995/1‑94 (MQ=60)
  CCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGG                                                                                           <  SRR4302544.1733560/94‑1 (MQ=60)
   CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGA                                                                                          >  SRR4302544.122678/1‑94 (MQ=60)
   CGGCAGTCCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGA                                                                                          <  SRR4302544.4337312/94‑1 (MQ=60)
     GCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAACGACCAAAATTGTTTGCCCCGTCGGACC                                                                                        >  SRR4302544.4131524/1‑94 (MQ=60)
      CAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCG                                                                                       >  SRR4302544.1667156/1‑94 (MQ=60)
       AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGCCCAAAATTGTTTGCACCGTCGAACCGA                                                                                      >  SRR4302544.685842/1‑94 (MQ=60)
       AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      <  SRR4302544.778746/94‑1 (MQ=60)
       AGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGA                                                                                      >  SRR4302544.873848/1‑94 (MQ=60)
         TGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAA                                                                                    <  SRR4302544.1148863/94‑1 (MQ=60)
            GCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACC                                                                                 >  SRR4302544.1924388/1‑94 (MQ=60)
            GCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACC                                                                                 <  SRR4302544.4296946/94‑1 (MQ=60)
             CCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCG                                                                                <  SRR4302544.4505508/94‑1 (MQ=60)
             CCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCG                                                                                >  SRR4302544.1668262/1‑94 (MQ=60)
             CCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCA                                                                                >  SRR4302544.4117133/1‑94 (MQ=60)
               CAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAA                                                                              <  SRR4302544.321526/94‑1 (MQ=60)
                ATAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.3394671/94‑1 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.3696725/94‑1 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.3845298/94‑1 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.3696724/94‑1 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             >  SRR4302544.4708003/1‑94 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.1284735/94‑1 (MQ=60)
                AGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAT                                                                             <  SRR4302544.1193362/94‑1 (MQ=60)
                 GAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATC                                                                            >  SRR4302544.3081490/1‑94 (MQ=60)
                 GAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATC                                                                            >  SRR4302544.2041901/1‑94 (MQ=60)
                  AAAGCAAGTTTCTCGCGTCCTTCTGAACTTAAAGACTAAGGCTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           <  SRR4302544.1261653/94‑1 (MQ=60)
                  AAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCT                                                                           >  SRR4302544.2607289/1‑94 (MQ=60)
                   AAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTG                                                                          >  SRR4302544.758965/1‑94 (MQ=60)
                   AAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTG                                                                          <  SRR4302544.2823663/94‑1 (MQ=60)
                   AAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTG                                                                          <  SRR4302544.193599/94‑1 (MQ=60)
                    AGCAAGTTTCTCCCATCCTTCACAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGA                                                                         >  SRR4302544.4546489/1‑94 (MQ=60)
                     GCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAG                                                                        >  SRR4302544.1474738/1‑94 (MQ=60)
                      CAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAG                                                                       >  SRR4302544.2125406/1‑94 (MQ=60)
                       AAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGA                                                                      >  SRR4302544.4573460/1‑94 (MQ=60)
                           TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  >  SRR4302544.2693117/1‑94 (MQ=60)
                           TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATG                                                                  <  SRR4302544.368435/94‑1 (MQ=60)
                            TCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGT                                                                 <  SRR4302544.3394625/94‑1 (MQ=60)
                             CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTT                                                                <  SRR4302544.563494/94‑1 (MQ=60)
                             CTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTT                                                                >  SRR4302544.2445683/1‑94 (MQ=60)
                               CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              <  SRR4302544.1897962/94‑1 (MQ=60)
                               CCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAG                                                              <  SRR4302544.1832830/94‑1 (MQ=60)
                                CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGTGCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302544.3266393/1‑94 (MQ=60)
                                CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302544.3266392/1‑94 (MQ=60)
                                CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGC                                                             >  SRR4302544.1563239/1‑94 (MQ=60)
                                  ATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTA                                                           <  SRR4302544.1654778/94‑1 (MQ=60)
                                   TCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAA                                                          <  SRR4302544.2019430/94‑1 (MQ=60)
                                   TCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAA                                                          <  SRR4302544.562229/94‑1 (MQ=60)
                                         TCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCT                                                    >  SRR4302544.1291638/1‑94 (MQ=60)
                                           AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  >  SRR4302544.4730117/1‑94 (MQ=60)
                                           AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGG                                                  >  SRR4302544.2587135/1‑94 (MQ=60)
                                              TTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACG                                               >  SRR4302544.2378588/1‑94 (MQ=60)
                                                AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCT                                             >  SRR4302544.3837504/1‑94 (MQ=60)
                                                AAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCT                                             <  SRR4302544.1183545/94‑1 (MQ=60)
                                                 AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGGGATGTTAGCTAAAATGCTGGACGCTG                                            <  SRR4302544.418020/94‑1 (MQ=60)
                                                 AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTG                                            >  SRR4302544.1496512/1‑94 (MQ=60)
                                                 AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTG                                            >  SRR4302544.4178157/1‑94 (MQ=60)
                                                  AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           >  SRR4302544.3770879/1‑94 (MQ=60)
                                                  AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           <  SRR4302544.1712933/94‑1 (MQ=60)
                                                  AGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGG                                           <  SRR4302544.1712932/94‑1 (MQ=60)
                                                   GACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGC                                          >  SRR4302544.2468819/1‑94 (MQ=60)
                                                      TAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATG                                       >  SRR4302544.2930389/1‑94 (MQ=60)
                                                       AAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGA                                      >  SRR4302544.462956/1‑94 (MQ=60)
                                                       AAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGA                                      >  SRR4302544.2391985/1‑94 (MQ=60)
                                                        AGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAA                                     >  SRR4302544.4134569/1‑94 (MQ=60)
                                                         GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    <  SRR4302544.2350173/94‑1 (MQ=60)
                                                         GACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAC                                    >  SRR4302544.3151951/1‑94 (MQ=60)
                                                          ACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACG                                   >  SRR4302544.4702816/1‑94 (MQ=60)
                                                           CTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGT                                  >  SRR4302544.1078429/1‑94 (MQ=60)
                                                            TGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTT                                 <  SRR4302544.382155/94‑1 (MQ=60)
                                                             GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                >  SRR4302544.3396289/1‑94 (MQ=60)
                                                             GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                >  SRR4302544.1168545/1‑94 (MQ=60)
                                                             GTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTA                                <  SRR4302544.978823/94‑1 (MQ=60)
                                                              TCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAT                               <  SRR4302544.2312565/94‑1 (MQ=60)
                                                               CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              <  SRR4302544.2997335/94‑1 (MQ=60)
                                                               CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              >  SRR4302544.556140/1‑94 (MQ=60)
                                                               CATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATG                              <  SRR4302544.205514/94‑1 (MQ=60)
                                                                ATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGC                             >  SRR4302544.4093150/1‑94 (MQ=60)
                                                                  GAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGT                           >  SRR4302544.1184434/1‑94 (MQ=60)
                                                                    AAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT                         >  SRR4302544.3803839/1‑94 (MQ=60)
                                                                    AAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT                         <  SRR4302544.4532604/94‑1 (MQ=60)
                                                                      AAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGA                       <  SRR4302544.1096722/94‑1 (MQ=60)
                                                                       AGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAA                      <  SRR4302544.4559908/94‑1 (MQ=60)
                                                                        GACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAC                     <  SRR4302544.4147755/94‑1 (MQ=60)
                                                                         ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACT                    <  SRR4302544.1690010/94‑1 (MQ=60)
                                                                         ACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACT                    >  SRR4302544.1447426/1‑94 (MQ=60)
                                                                          CCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTT                   >  SRR4302544.463931/1‑94 (MQ=60)
                                                                               ATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTC              >  SRR4302544.968739/1‑94 (MQ=60)
                                                                                TTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA             <  SRR4302544.2993884/94‑1 (MQ=60)
                                                                                TTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA             <  SRR4302544.1209862/94‑1 (MQ=60)
                                                                                 TGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAT            <  SRR4302544.2394481/94‑1 (MQ=60)
                                                                                 TGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAT            <  SRR4302544.1142954/94‑1 (MQ=60)
                                                                                    TTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAATATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGT         <  SRR4302544.407743/94‑1 (MQ=60)
                                                                                     TGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTG        <  SRR4302544.1761526/94‑1 (MQ=60)
                                                                                       CACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC      >  SRR4302544.1106367/1‑94 (MQ=60)
                                                                                        ACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACT     >  SRR4302544.3191601/1‑94 (MQ=60)
                                                                                        ACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACT     <  SRR4302544.2188618/94‑1 (MQ=60)
                                                                                          CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   >  SRR4302544.3290200/1‑94 (MQ=60)
                                                                                          CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   <  SRR4302544.1428238/94‑1 (MQ=60)
                                                                                          CGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT   >  SRR4302544.3012391/1‑94 (MQ=60)
                                                                                           GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  <  SRR4302544.542817/94‑1 (MQ=60)
                                                                                           GTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  <  SRR4302544.3801558/94‑1 (MQ=60)
                                                                                           |                                                                                             
TTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATG  >  NC_000913/1753658‑1753842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑