Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,791,298 T→C T95T (ACA→ACG)  btuD ← ATP‑binding component of vitamin B12 transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,791,2980TC96.2% 399.1 / 3.6 133T95T (ACA→ACG) btuDATP‑binding component of vitamin B12 transport system
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (55/73);  total (58/75)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01

Only 100 of 139 total aligned reads displayed.

ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791208‑1791387
                                                                                          |                                                                                         
aTTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTGCGCGTTTTATCGTGCTGATGCAgcgt                                                                                         <  1:1576130/93‑1 (MQ=255)
 ttGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTc                                                                                        >  2:1807310/1‑93 (MQ=255)
 ttGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTc                                                                                        <  1:2178149/93‑1 (MQ=255)
  tGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCa                                                                                       <  2:845976/93‑1 (MQ=255)
     tGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGt                                                                                    >  1:1055797/1‑93 (MQ=255)
     tGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGt                                                                                    >  1:1216613/1‑93 (MQ=255)
     tGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGt                                                                                    <  1:940812/93‑1 (MQ=255)
      tcTACGTCCGAGTTTGTCATCAAGGGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTa                                                                                   <  1:4032492/92‑1 (MQ=255)
       cTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAg                                                                                  >  2:3539370/1‑93 (MQ=255)
         aCGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTg                                                                                >  2:2604006/1‑93 (MQ=255)
           gTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGcc                                                                              <  1:1852311/93‑1 (MQ=255)
            tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             >  2:2032792/1‑93 (MQ=255)
            tCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             <  1:355413/93‑1 (MQ=255)
            gccGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCa                                                                             <  1:1126118/92‑1 (MQ=255)
              cGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGa                                                                           <  2:4109199/93‑1 (MQ=255)
               gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAc                                                                          <  2:2902458/93‑1 (MQ=255)
               gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAc                                                                          <  2:2408956/93‑1 (MQ=255)
                aGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGAcc                                                                         <  2:4166319/93‑1 (MQ=255)
                 gTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCg                                                                        >  1:2390982/1‑93 (MQ=255)
                 gTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCg                                                                        >  1:3484547/1‑93 (MQ=255)
                 gTTTGTCATCAAGAGCCAACGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCg                                                                        >  1:191911/1‑93 (MQ=255)
                  tttGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCgg                                                                       >  2:129976/1‑93 (MQ=255)
                   ttGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGc                                                                      >  1:861976/1‑93 (MQ=255)
                     gTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGt                                                                    <  1:1492062/93‑1 (MQ=255)
                        atcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGc                                                                 >  2:3413832/1‑93 (MQ=255)
                         tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                >  2:2841373/1‑93 (MQ=255)
                            agagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAc                                                             <  1:4320808/93‑1 (MQ=255)
                             gagCCAGCGCTCCTGCGACATCATTCAGGAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            <  1:338679/93‑1 (MQ=255)
                             gagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            <  2:3718105/93‑1 (MQ=255)
                             gagCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            <  2:1051486/93‑1 (MQ=255)
                             gagCCAGCGCCCCTGCGACATCATTCAGTAGTTAGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAAcg                                                            >  2:2520614/1‑93 (MQ=255)
                                  aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                       >  2:2449591/1‑93 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   >  1:737636/1‑93 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   <  1:3220425/93‑1 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   >  1:2716324/1‑93 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   >  2:1686310/1‑93 (MQ=255)
                                      ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                   >  1:4248781/1‑93 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 <  1:2562305/93‑1 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 <  2:1132642/93‑1 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 >  1:2595573/1‑93 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgc                                                 >  1:1600809/1‑93 (MQ=255)
                                        ccTGCGACATCATTCAGTAGTTCGGGACGTGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGAGTTGCAAACGGCGGGGTctac                                                 >  1:3147671/1‑91 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               <  1:1265413/93‑1 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               >  2:2865176/1‑93 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               <  1:712971/93‑1 (MQ=255)
                                          tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                               <  2:819283/93‑1 (MQ=255)
                                           gCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgt                                              <  1:2678867/93‑1 (MQ=255)
                                             gACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                            <  2:2696784/93‑1 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  2:3752123/93‑1 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  2:496437/93‑1 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  1:2104214/93‑1 (MQ=255)
                                              aCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttgt                                           <  1:2730626/93‑1 (MQ=255)
                                                atcatTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGa                                         >  1:1717239/1‑93 (MQ=255)
                                                  catTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGaaa                                       <  1:814601/93‑1 (MQ=255)
                                                  catTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGaaa                                       <  2:131165/93‑1 (MQ=255)
                                                    ttCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                     >  2:4265755/1‑93 (MQ=255)
                                                    ttCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                     <  1:4517544/93‑1 (MQ=255)
                                                     tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGaa                                    >  2:112629/1‑92 (MQ=255)
                                                     tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                    >  1:2518602/1‑93 (MQ=255)
                                                     tCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGGTGTGACAGAt                                    >  2:2157808/1‑93 (MQ=255)
                                                       agtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAg                                  <  2:2749718/93‑1 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 >  1:516761/1‑93 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 >  1:3456837/1‑93 (MQ=255)
                                                        gtagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  2:4639118/93‑1 (MQ=255)
                                                        gtagtTCCGTACGCGGTTTATCGCGCAGATGCATTGTCAGGTAGTGCCAGACCGGCTTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAgg                                 <  1:1347993/93‑1 (MQ=255)
                                                         tagtTTGGTCCGCTTTTTATGGTGCGGATGCAGCGTGAGGTAGTGCCAGACGGGCGGTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  2:520110/93‑1 (MQ=255)
                                                         tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAggg                                >  1:1603620/1‑92 (MQ=255)
                                                         tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  1:146549/93‑1 (MQ=255)
                                                         tagtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  1:4720264/93‑1 (MQ=255)
                                                         tagtTCGGTACGCGGTCTATCGTGCTGATGCAGCGTCAGGCAGTGCCAGACCGGCGTGGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                <  2:3898232/93‑1 (MQ=255)
                                                           gtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcgc                              <  2:1882231/93‑1 (MQ=255)
                                                           gtTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcgc                              <  2:285045/93‑1 (MQ=255)
                                                             tCGGTACGAGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGa                            >  2:489544/1‑93 (MQ=255)
                                                               ggTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATg                          <  2:336668/93‑1 (MQ=255)
                                                               ggTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATg                          >  1:2046026/1‑93 (MQ=255)
                                                                gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         <  2:3709958/93‑1 (MQ=255)
                                                                gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         <  1:1372620/93‑1 (MQ=255)
                                                                gTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGc                         <  1:2860257/93‑1 (MQ=255)
                                                                 tACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCa                        >  2:2386723/1‑93 (MQ=255)
                                                                 tACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCa                        >  1:2423905/1‑93 (MQ=255)
                                                                   cgcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                      <  1:3488153/93‑1 (MQ=255)
                                                                    tggTTGTATCGTGCGGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcg                     <  1:3193111/91‑1 (MQ=255)
                                                                    gcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcg                     <  2:3053562/93‑1 (MQ=255)
                                                                    gcgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcg                     >  1:3832941/1‑93 (MQ=255)
                                                                     cgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcgc                    <  2:1308850/93‑1 (MQ=255)
                                                                     cgTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcgc                    <  2:31311/93‑1 (MQ=255)
                                                                       ttttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGa                  <  2:253373/93‑1 (MQ=255)
                                                                       ttttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGa                  <  2:4703775/93‑1 (MQ=255)
                                                                        tttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAg                 <  1:434170/93‑1 (MQ=255)
                                                                         ttATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGt                >  2:2314389/1‑93 (MQ=255)
                                                                          tATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtt               <  1:4272778/93‑1 (MQ=255)
                                                                          tATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGtt               >  1:1987356/1‑93 (MQ=255)
                                                                          tATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGGCTGCTGTTGTGAAAGATAGGCGCGATGGAGggcgggct               >  2:1073401/1‑89 (MQ=255)
                                                                                gCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg         >  2:2270695/1‑93 (MQ=255)
                                                                                 cTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc        <  2:1659999/93‑1 (MQ=255)
                                                                                   gATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg      <  1:3508121/93‑1 (MQ=255)
                                                                                      gCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAcc   <  2:1373282/93‑1 (MQ=255)
                                                                                       cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  2:1751509/93‑1 (MQ=255)
                                                                                       cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  1:1708732/93‑1 (MQ=255)
                                                                                       cAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCa  <  1:2766180/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCA  >  NC_000913/1791208‑1791387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCT  >  NC_000913/1791208‑1791391
                                                                                          |                                                                                             
ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTGCGCGTTTTATCGTGCTGATGCAGCGTC                                                                                            <  SRR4302544.1591712/94‑1 (MQ=60)
 TTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCA                                                                                           <  SRR4302544.2198780/94‑1 (MQ=60)
   GGTGCCACGTCCGATTTTGTCATCAAGAGCCAGCGTCCCTGCTACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTTATGCAGTGTCAGG                                                                                         <  SRR4302544.2496118/94‑1 (MQ=60)
    GTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGT                                                                                        >  SRR4302544.1228653/1‑94 (MQ=60)
    GTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGT                                                                                        >  SRR4302544.1066270/1‑94 (MQ=60)
     TGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTA                                                                                       <  SRR4302544.950075/94‑1 (MQ=60)
      TCTACGTCCGAGTTTGTCATCAAGGGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAG                                                                                      <  SRR4302544.4072660/94‑1 (MQ=60)
       GTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGT                                                                                     <  SRR4302544.4333444/94‑1 (MQ=60)
       CTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGT                                                                                     <  SRR4302544.2029519/94‑1 (MQ=60)
           GTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCA                                                                                 <  SRR4302544.1870232/94‑1 (MQ=60)
            TCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAG                                                                                <  SRR4302544.359056/94‑1 (MQ=60)
            GCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAG                                                                                <  SRR4302544.1137339/94‑1 (MQ=60)
               GAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACC                                                                             <  SRR4302544.1701287/94‑1 (MQ=60)
                AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                            >  SRR4302544.3517757/1‑94 (MQ=60)
                AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                            >  SRR4302544.3517758/1‑94 (MQ=60)
                AGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                            >  SRR4302544.2413631/1‑94 (MQ=60)
                AGTTTGTCATCAAGAGCCAACGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCG                                                                            >  SRR4302544.193836/1‑94 (MQ=60)
                  TTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGC                                                                          >  SRR4302544.870505/1‑94 (MQ=60)
                     GTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTT                                                                       <  SRR4302544.1506818/94‑1 (MQ=60)
                            AGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACG                                                                <  SRR4302544.4364819/94‑1 (MQ=60)
                             GAGCCAGCGCTCCTGCGACATCATTCAGGAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGG                                                               <  SRR4302544.342191/94‑1 (MQ=60)
                                     GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                       >  SRR4302544.744853/1‑94 (MQ=60)
                                     GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                       >  SRR4302544.744852/1‑94 (MQ=60)
                                     GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                       >  SRR4302544.4292101/1‑94 (MQ=60)
                                     GCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCT                                                       >  SRR4302544.2742470/1‑94 (MQ=60)
                                      CCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTG                                                      <  SRR4302544.3251154/94‑1 (MQ=60)
                                       CCCTGCGACATCATTCAGTAGTTCGGTACGTGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGC                                                     >  SRR4302544.3177457/1‑94 (MQ=60)
                                       CCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGC                                                     >  SRR4302544.1616596/1‑94 (MQ=60)
                                       CCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGC                                                     >  SRR4302544.2620531/1‑94 (MQ=60)
                                       CCCTGCGACATCATTCAGTAGTTCGGGACGTGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGAGTTGCAAACGGCGGGGTCTAC                                                     >  SRR4302544.3177596/1‑94 (MQ=60)
                                        CCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCT                                                    <  SRR4302544.2586916/94‑1 (MQ=60)
                                          TGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGT                                                  <  SRR4302544.1277862/94‑1 (MQ=60)
                                          TGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGT                                                  <  SRR4302544.720033/94‑1 (MQ=60)
                                           GCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTT                                                 <  SRR4302544.2704537/94‑1 (MQ=60)
                                            CGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTG                                                >  SRR4302544.1519314/1‑94 (MQ=60)
                                              ACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTG                                              <  SRR4302544.2756944/94‑1 (MQ=60)
                                              ACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTG                                              <  SRR4302544.2124457/94‑1 (MQ=60)
                                               CATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGA                                             >  SRR4302544.1734123/1‑94 (MQ=60)
                                                  CATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAG                                          <  SRR4302544.275042/94‑1 (MQ=60)
                                                  CATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAG                                          <  SRR4302544.822555/94‑1 (MQ=60)
                                                    TTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAT                                        >  SRR4302544.2542795/1‑94 (MQ=60)
                                                    TTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAT                                        <  SRR4302544.4562782/94‑1 (MQ=60)
                                                       AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCGGGTAGTGCCAGACCGGGGTTGCAcctggcgtggttggcggttgtgaagattagg                                     >  SRR4302544.521871/1‑63 (MQ=60)
                                                       AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                     >  SRR4302544.3489832/1‑94 (MQ=60)
                                                       AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                     >  SRR4302544.521872/1‑94 (MQ=60)
                                                       AGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGG                                     >  SRR4302544.3231280/1‑94 (MQ=60)
                                                        GTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGG                                    >  SRR4302544.1619428/1‑94 (MQ=60)
                                                        GTAGTTCCGTACGCGGTTTATCGCGCAGATGCATTGTCAGGTAGTGCCAGACCGGCTTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGC                                    <  SRR4302544.1361348/94‑1 (MQ=60)
                                                         TAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCG                                   <  SRR4302544.4766871/94‑1 (MQ=60)
                                                         TAGTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCG                                   <  SRR4302544.147951/94‑1 (MQ=60)
                                                           GTTCGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCG                                 >  SRR4302544.2169495/1‑94 (MQ=60)
                                                              CGGTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATG                              >  SRR4302544.2065745/1‑94 (MQ=60)
                                                                GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                            <  SRR4302544.2887865/94‑1 (MQ=60)
                                                                GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                            >  SRR4302544.2446977/1‑94 (MQ=60)
                                                                GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                            <  SRR4302544.1386298/94‑1 (MQ=60)
                                                                GTACGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCA                            >  SRR4302544.1252582/1‑94 (MQ=60)
                                                                   CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                         <  SRR4302544.974417/94‑1 (MQ=60)
                                                                   CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                         <  SRR4302544.3521408/94‑1 (MQ=60)
                                                                   CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                         >  SRR4302544.3870940/1‑94 (MQ=60)
                                                                   CGCGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCG                         <  SRR4302544.3521409/94‑1 (MQ=60)
                                                                    tggttgTATCGTGCGGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGC                        <  SRR4302544.3223543/88‑1 (MQ=60)
                                                                     CGTTTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCG                       <  SRR4302544.1423231/94‑1 (MQ=60)
                                                                      GTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGA                      >  SRR4302544.4451584/1‑94 (MQ=60)
                                                                        TTTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGT                    <  SRR4302544.438549/94‑1 (MQ=60)
                                                                         TTATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTT                   >  SRR4302544.2006649/1‑94 (MQ=60)
                                                                          TATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTT                  <  SRR4302544.4316399/94‑1 (MQ=60)
                                                                           ATCGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTT                 >  SRR4302544.3966013/1‑94 (MQ=60)
                                                                             CGTGCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGT               <  SRR4302544.592661/94‑1 (MQ=60)
                                                                                GCTGATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGC            <  SRR4302544.1840530/94‑1 (MQ=60)
                                                                                   GATGCAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGA         <  SRR4302544.3541585/94‑1 (MQ=60)
                                                                                     TGCAGCGTCAGGTAGTGCCAGACCGGCGGTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACC       <  SRR4302544.2635698/94‑1 (MQ=60)
                                                                                       CAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCAT     <  SRR4302544.2792784/94‑1 (MQ=60)
                                                                                       CAGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCAT     <  SRR4302544.1725565/94‑1 (MQ=60)
                                                                                        AGCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATG    >  SRR4302544.305454/1‑94 (MQ=60)
                                                                                         GCGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGC   <  SRR4302544.418201/94‑1 (MQ=60)
                                                                                          CGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCT  >  SRR4302544.4593508/1‑94 (MQ=60)
                                                                                          CGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCT  >  SRR4302544.2000247/1‑94 (MQ=60)
                                                                                          |                                                                                             
ATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCT  >  NC_000913/1791208‑1791391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑