Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,830,747 T→C L99P (CTG→CCG)  xthA → exonuclease III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,7470TC97.8% 407.4 / ‑0.7 134L99P (CTG→CCG) xthAexonuclease III
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (72/59);  total (73/61)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 149 total aligned reads displayed.

CAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTT  >  NC_000913/1830658‑1830836
                                                                                         |                                                                                         
cAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCAccgct                                                                                        <  1:3977473/93‑1 (MQ=255)
cAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCAccgcg                                                                                        >  1:1605107/1‑92 (MQ=255)
 aaaGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATTCCCTCCccgctg                                                                                       >  2:1424924/1‑93 (MQ=255)
 aaaGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCAccgctg                                                                                       >  2:3933586/1‑93 (MQ=255)
 aaaGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCAccgctg                                                                                       >  1:1318750/1‑93 (MQ=255)
 aaaGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGACGCAGCGGCGGATTATTATGGCGGAAATCCCCTCAccgctg                                                                                       >  1:1983163/1‑93 (MQ=255)
  aaGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTgg                                                                                      >  2:1137135/1‑93 (MQ=255)
    gagaCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGt                                                                                    >  1:1865047/1‑93 (MQ=255)
    gagaCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGt                                                                                    >  2:3873847/1‑93 (MQ=255)
      gaCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTaa                                                                                  >  1:3906998/1‑93 (MQ=255)
      gaCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGGaa                                                                                  >  1:2886317/1‑93 (MQ=255)
       aCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAAt                                                                                 <  2:230559/93‑1 (MQ=255)
        cGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATg                                                                                <  1:1593158/93‑1 (MQ=255)
           cGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCa                                                                             >  2:418877/1‑93 (MQ=255)
            gATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCAc                                                                            <  1:4234958/93‑1 (MQ=255)
             aTTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGGGGAAATCCCCCCACCGCTGGGTAATGTCAcc                                                                           >  2:1470309/1‑93 (MQ=255)
             aTTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCAcc                                                                           <  2:1103151/93‑1 (MQ=255)
             aTTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGGAATGTCAcc                                                                           >  2:3410361/1‑93 (MQ=255)
             aTTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAAACCCCTCACCGCTGGGTAATGTCAcc                                                                           >  1:4520778/1‑93 (MQ=255)
               tGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGt                                                                         <  1:4360037/93‑1 (MQ=255)
               tGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGt                                                                         >  1:4438686/1‑93 (MQ=255)
               tGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGt                                                                         <  2:2191664/93‑1 (MQ=255)
               tGCCGTGCGTCGCGGCTGTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGt                                                                         <  1:4360038/93‑1 (MQ=255)
                gCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGCCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTg                                                                        <  2:178450/93‑1 (MQ=255)
                 ccGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGa                                                                       <  1:2287775/93‑1 (MQ=255)
                 ccGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGGAATGTCCCCGTGa                                                                       >  2:111833/1‑93 (MQ=255)
                   gTGCGTCGCGGCTTTCCCGGTGACGAGGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCACCTACCGCTGGGTGATGTCACCGTGATc                                                                     >  2:2197125/1‑93 (MQ=255)
                   gTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATc                                                                     >  2:2732153/1‑93 (MQ=255)
                   gTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATc                                                                     >  2:2721547/1‑93 (MQ=255)
                   gTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATc                                                                     >  2:847530/1‑93 (MQ=255)
                      cGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAAc                                                                  >  1:667953/1‑93 (MQ=255)
                      cGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAAc                                                                  >  2:2583539/1‑93 (MQ=255)
                       gTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACg                                                                 >  1:3550318/1‑93 (MQ=255)
                       gTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACg                                                                 <  1:570893/93‑1 (MQ=255)
                       gTCGCGGCTTTCCCGGCGACGACGAAAAGGCGCAGCGGCGGATTATTATGGCGGAAATACCCTTACCGCTGGGTAATGTCACCGTGATCaaag                                                                 >  2:4069472/1‑91 (MQ=255)
                        tCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACgg                                                                >  2:262674/1‑93 (MQ=255)
                        tCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACgg                                                                >  2:869990/1‑93 (MQ=255)
                           cgGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTa                                                             <  2:3041044/93‑1 (MQ=255)
                            ggCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTAc                                                            <  2:4341269/93‑1 (MQ=255)
                             gCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACt                                                           >  1:58372/1‑93 (MQ=255)
                              cTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACtt                                                          <  1:2882569/93‑1 (MQ=255)
                               tttCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTc                                                         <  1:263395/93‑1 (MQ=255)
                               tttCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTc                                                         >  2:478863/1‑93 (MQ=255)
                                ttCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTcc                                                        <  2:943984/93‑1 (MQ=255)
                                 tCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCCCCGCTGGGTAATGTCACCGTGATCAACGGTTACTTccc                                                       <  1:4146254/93‑1 (MQ=255)
                                  cccGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGTAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCg                                                      <  1:665481/93‑1 (MQ=255)
                                    cGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCa                                                    <  2:755504/93‑1 (MQ=255)
                                    cGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCa                                                    >  1:1423167/1‑93 (MQ=255)
                                     ggTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAg                                                   >  1:3630927/1‑93 (MQ=255)
                                      gTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAgg                                                  >  1:1257124/1‑93 (MQ=255)
                                      gTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAgg                                                  <  2:2461580/93‑1 (MQ=255)
                                       ggacggcGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAggg                                                 <  1:101434/92‑1 (MQ=255)
                                        gacgacGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGt                                                <  2:3634986/93‑1 (MQ=255)
                                         acgacgAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTg                                               >  1:4355161/1‑93 (MQ=255)
                                             cgaAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAg                                           >  1:2274915/1‑93 (MQ=255)
                                               aaGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGcc                                         >  1:334632/1‑93 (MQ=255)
                                                agagGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCcg                                        >  1:1690177/1‑93 (MQ=255)
                                                 gagGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCcgc                                       >  2:1286199/1‑93 (MQ=255)
                                                 gagGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCcgc                                       <  2:762565/93‑1 (MQ=255)
                                                 gagGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCcgc                                       <  2:3393232/93‑1 (MQ=255)
                                                  agGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCcgcg                                      >  2:1506484/1‑93 (MQ=255)
                                                   ggCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGa                                     >  1:410443/1‑93 (MQ=255)
                                                    tcgcAGCGGGGGATTATTATGTCGGAAATCCCCTCACCGCTGGGGAATGTCACCGTGATCAACGGTTACTTCCCGCAGGTTGAAAGCcgcgcc                                    <  2:3384765/92‑3 (MQ=255)
                                                    gcgcAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGAc                                    >  1:2521773/1‑93 (MQ=255)
                                                     cgcAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGAcc                                   >  1:1045355/1‑93 (MQ=255)
                                                       cAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCAt                                 <  2:426895/93‑1 (MQ=255)
                                                       cAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCAt                                 <  2:31356/93‑1 (MQ=255)
                                                         gcggcgGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATcc                               <  1:4698905/93‑1 (MQ=255)
                                                         gcggcgGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATcc                               >  2:286933/1‑93 (MQ=255)
                                                         gcggcgGATTATTAT‑GCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCg                              >  2:2881750/1‑93 (MQ=255)
                                                           ggcggATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGa                             <  1:905203/93‑1 (MQ=255)
                                                           ggcggATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGa                             <  1:639344/93‑1 (MQ=255)
                                                            gcggATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGAt                            <  1:2536468/93‑1 (MQ=255)
                                                               gATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATaaa                         >  2:4275503/1‑93 (MQ=255)
                                                               gATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATaaa                         >  2:302696/1‑93 (MQ=255)
                                                                attattATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATaaaa                        >  1:2965476/1‑93 (MQ=255)
                                                                 ttattaTGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAAt                       >  1:636559/1‑93 (MQ=255)
                                                                   attatGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTc                     >  1:2471582/1‑93 (MQ=255)
                                                                    ttatGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTcc                    <  2:1788865/93‑1 (MQ=255)
                                                                     tatGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTccc                   <  1:2321234/93‑1 (MQ=255)
                                                                      atGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCg                  <  1:978462/93‑1 (MQ=255)
                                                                       tGGCGGAAATCCCCTCACTGCTGGGGAATGGCACCGTGATCAACGGTTACTTCCCGCAGGGGGAAAGCCGCGACCATCCGATAAAATTCCCgg                 <  2:4003922/93‑1 (MQ=255)
                                                                            gAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCaaaa            <  1:188538/93‑1 (MQ=255)
                                                                            gAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCaaaa            <  2:2067159/93‑1 (MQ=255)
                                                                            gAAACCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCAGCCGATAAAATTCCCGGCaaaa            <  2:3651089/93‑1 (MQ=255)
                                                                             aaaTCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAg           <  2:3202899/93‑1 (MQ=255)
                                                                              aaTCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgc          >  2:3879362/1‑93 (MQ=255)
                                                                              aaTCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgc          <  2:2501229/93‑1 (MQ=255)
                                                                              aaTCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgc          <  2:658480/93‑1 (MQ=255)
                                                                               aTCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcg         >  1:2014388/1‑93 (MQ=255)
                                                                                tCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcgc        >  1:4726619/1‑93 (MQ=255)
                                                                                tCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcgc        >  2:847254/1‑93 (MQ=255)
                                                                                 ccccTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCa       <  2:4419901/93‑1 (MQ=255)
                                                                                 ccccTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCa       <  1:3395106/93‑1 (MQ=255)
                                                                                  cccTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAg      >  2:3245948/1‑93 (MQ=255)
                                                                                   ccTCACCGCTGGGTAATGTCCCCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGCCCATCCGATAAAATTCCCGGCAAAAGCGCAGt     <  1:171025/93‑1 (MQ=255)
                                                                                   ccTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGt     <  1:3183773/93‑1 (MQ=255)
                                                                                   ccTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGt     <  2:2524733/93‑1 (MQ=255)
                                                                                    cTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGtt    <  2:1877397/93‑1 (MQ=255)
                                                                                     tCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGttt   >  1:2418842/1‑93 (MQ=255)
                                                                                         |                                                                                         
CAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTT  >  NC_000913/1830658‑1830836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCA  >  NC_000913/1830655‑1830840
                                                                                            |                                                                                             
GACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCG                                                                                              <  SRR4302544.3742599/94‑1 (MQ=60)
   CAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTG                                                                                           <  SRR4302544.4017056/94‑1 (MQ=60)
   CAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTG                                                                                           >  SRR4302544.1331714/1‑94 (MQ=60)
   CAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGACGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTG                                                                                           >  SRR4302544.2002422/1‑94 (MQ=60)
   CAAAGAGACGCCGATGGCCGTGCGTCGCGGCTTACCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTG                                                                                           <  SRR4302544.1997973/94‑1 (MQ=60)
    AAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGG                                                                                          >  SRR4302544.419933/1‑94 (MQ=60)
     AAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGG                                                                                         <  SRR4302544.2322951/94‑1 (MQ=60)
      AGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTGGGT                                                                                        >  SRR4302544.4086234/1‑94 (MQ=60)
      AGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGT                                                                                        >  SRR4302544.1883070/1‑94 (MQ=60)
        AGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAA                                                                                      >  SRR4302544.3945816/1‑94 (MQ=60)
        AGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGGAA                                                                                      >  SRR4302544.2914152/1‑94 (MQ=60)
           CGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGT                                                                                   <  SRR4302544.1608856/94‑1 (MQ=60)
             CCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCC                                                                                 >  SRR4302544.625109/1‑94 (MQ=60)
               GATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACC                                                                               <  SRR4302544.4278124/94‑1 (MQ=60)
               GATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGAAATGTCACC                                                                               >  SRR4302544.1625240/1‑94 (MQ=60)
               GATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAAACCCCTCACCGCTGGGTAATGTCACC                                                                               >  SRR4302544.4566039/1‑94 (MQ=60)
                 TTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGT                                                                             >  SRR4302544.4483440/1‑94 (MQ=60)
                  TGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTG                                                                            <  SRR4302544.4404286/94‑1 (MQ=60)
                  TGCCGTGCGTCGCGGCTGTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTG                                                                            <  SRR4302544.4404287/94‑1 (MQ=60)
                   GCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTTA                                                                           <  SRR4302544.2964362/94‑1 (MQ=60)
                    CCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGAT                                                                          <  SRR4302544.2309345/94‑1 (MQ=60)
                        GCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAAC                                                                      >  SRR4302544.674549/1‑94 (MQ=60)
                        GCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAAC                                                                      >  SRR4302544.3455204/1‑94 (MQ=60)
                         CGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACG                                                                     >  SRR4302544.3584321/1‑94 (MQ=60)
                          GTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGG                                                                    <  SRR4302544.576473/94‑1 (MQ=60)
                               GGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACT                                                               >  SRR4302544.58965/1‑94 (MQ=60)
                                 CTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTC                                                             <  SRR4302544.2910375/94‑1 (MQ=60)
                                  TTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCC                                                            <  SRR4302544.266181/94‑1 (MQ=60)
                                    TCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCCCCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCG                                                          <  SRR4302544.4187302/94‑1 (MQ=60)
                                     CCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGTAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGC                                                         <  SRR4302544.672054/94‑1 (MQ=60)
                                     CCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCATGATCAACGGTTACTTCCCGC                                                         <  SRR4302544.672051/94‑1 (MQ=60)
                                      CCGGTGACGACGAAGAGGCGCAGCGGCGGCATATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCG                                                        >  SRR4302544.590023/1‑94 (MQ=60)
                                      CCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCA                                                        >  SRR4302544.1437328/1‑94 (MQ=60)
                                       CGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATTCCCTCACCGCCGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAG                                                       >  SRR4302544.3667101/1‑94 (MQ=60)
                                       CGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAG                                                       >  SRR4302544.3667100/1‑94 (MQ=60)
                                        GGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGG                                                      >  SRR4302544.1269496/1‑94 (MQ=60)
                                          GGACGGCGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGT                                                    <  SRR4302544.102362/94‑1 (MQ=60)
                                           GACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTG                                                   >  SRR4302544.4399385/1‑94 (MQ=60)
                                             CGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGGGAA                                                 >  SRR4302544.2559648/1‑94 (MQ=60)
                                               ACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAG                                               >  SRR4302544.2296330/1‑94 (MQ=60)
                                               ACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAG                                               >  SRR4302544.2296329/1‑94 (MQ=60)
                                                 GAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCC                                             >  SRR4302544.338108/1‑94 (MQ=60)
                                                 GAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCC                                             <  SRR4302544.1528263/94‑1 (MQ=60)
                                                  AAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCG                                            >  SRR4302544.1706848/1‑94 (MQ=60)
                                                     AGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGA                                         >  SRR4302544.414627/1‑94 (MQ=60)
                                                      GGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGAC                                        >  SRR4302544.2545998/1‑94 (MQ=60)
                                                       GCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACC                                       >  SRR4302544.1055721/1‑94 (MQ=60)
                                                         GCAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCAT                                     <  SRR4302544.1743071/94‑1 (MQ=60)
                                                          CAGCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATC                                    >  SRR4302544.4774990/1‑94 (MQ=60)
                                                           AGCGGCGGATTATTATGGCGGAAATCCCCTGACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCC                                   >  SRR4302544.4591147/1‑94 (MQ=60)
                                                            GCGGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCG                                  <  SRR4302544.4745282/94‑1 (MQ=60)
                                                              GGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGAT                                <  SRR4302544.645646/94‑1 (MQ=60)
                                                              GGCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGAT                                <  SRR4302544.914159/94‑1 (MQ=60)
                                                               GCGGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATA                               <  SRR4302544.2560833/94‑1 (MQ=60)
                                                                 GGATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAA                             >  SRR4302544.2464778/1‑94 (MQ=60)
                                                                  GATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAA                            >  SRR4302544.2993927/1‑94 (MQ=60)
                                                                  GATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAA                            >  SRR4302544.1306289/1‑94 (MQ=60)
                                                                   ATTATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAAT                           >  SRR4302544.642832/1‑94 (MQ=60)
                                                                   ATTATTATGGCGGAAATACCCTCACCGCTGGGGAATGACACCGTGATCAACGGGTACTTCCCGCAGGGTGAGAGCCGCGACCATCCGATAAAAT                           >  SRR4302544.3160508/1‑94 (MQ=60)
                                                                     TATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTC                         >  SRR4302544.2495211/1‑94 (MQ=60)
                                                                      ATTATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCC                        >  SRR4302544.4403728/1‑94 (MQ=60)
                                                                        TATGGCGGAAATCCCCTCGCCGCGGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCG                      <  SRR4302544.4453096/94‑1 (MQ=60)
                                                                        TATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCG                      <  SRR4302544.2343228/94‑1 (MQ=60)
                                                                         ATGGCGGAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGG                     <  SRR4302544.988047/94‑1 (MQ=60)
                                                                               GAAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAG               <  SRR4302544.190418/94‑1 (MQ=60)
                                                                                AAATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGCGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGC              >  SRR4302544.989002/1‑94 (MQ=60)
                                                                                 AATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCG             >  SRR4302544.2033962/1‑94 (MQ=60)
                                                                                  ATCCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGC            >  SRR4302544.4773268/1‑94 (MQ=60)
                                                                                    CCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAG          >  SRR4302544.3251868/1‑94 (MQ=60)
                                                                                    CCCCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAG          <  SRR4302544.3427571/94‑1 (MQ=60)
                                                                                      CCTCACCGCTGGGTAATGTCCCCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGCCCATCCGATAAAATTCCCGGCAAAAGCGCAGTT        <  SRR4302544.172714/94‑1 (MQ=60)
                                                                                      CCTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTT        <  SRR4302544.3214073/94‑1 (MQ=60)
                                                                                       CTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGCCCATCCGATAAAATTCCCGGCAAAAGCGCAGTTT       <  SRR4302544.2694713/94‑1 (MQ=60)
                                                                                       CTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTT       >  SRR4302544.2441875/1‑94 (MQ=60)
                                                                                       CTCACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTT       >  SRR4302544.757920/1‑94 (MQ=60)
                                                                                         CACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTA     >  SRR4302544.1670345/1‑94 (MQ=60)
                                                                                         CACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGCTTTA     >  SRR4302544.2300179/1‑94 (MQ=60)
                                                                                          ACCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTAT    >  SRR4302544.2163202/1‑94 (MQ=60)
                                                                                           CCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATC   >  SRR4302544.3022774/1‑94 (MQ=60)
                                                                                           CCGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATA   >  SRR4302544.935406/1‑94 (MQ=60)
                                                                                            CGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCA  <  SRR4302544.4280804/94‑1 (MQ=60)
                                                                                            CGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCA  >  SRR4302544.3996517/1‑94 (MQ=60)
                                                                                            |                                                                                             
GACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCA  >  NC_000913/1830655‑1830840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑