Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,298	A→G	K146K (AAA→AAG)	sppA →	protease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,298	0	A	G	99.3%	438.7 / NA	136	K146K (AAA→AAG)	sppA	protease IV
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (58/77); total (59/77)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGT  >  NC_000913/1847208‑1847387

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTGTGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        >  2:2257409/1‑93 (MQ=255)

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        <  2:793682/93‑1 (MQ=255)

 gtgATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCg                                                                                        >  2:2257410/1‑93 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      <  1:1361913/93‑1 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      >  2:3062596/1‑93 (MQ=255)

   gATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGt                                                                                      <  1:2214334/93‑1 (MQ=255)

    aTGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGtt                                                                                     <  1:687870/93‑1 (MQ=255)

     tGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGttt                                                                                    <  1:1464356/93‑1 (MQ=255)

      ggATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTa                                                                                   <  2:370090/93‑1 (MQ=255)

      gcagcTGAAAAGCTTCGCAGGCGGCGTCCAACCGTCGATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTa                                                                                   <  2:370089/89‑1 (MQ=255)

        aTCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATg                                                                                 <  2:1489838/93‑1 (MQ=255)

        aTCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATg                                                                                 >  1:138700/1‑93 (MQ=255)

          cTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGcc                                                                               <  2:2747078/93‑1 (MQ=255)

           tGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCg                                                                              >  2:975452/1‑93 (MQ=255)

            gAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGt                                                                             >  1:2563922/1‑93 (MQ=255)

              aaaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTg                                                                           <  1:3572373/93‑1 (MQ=255)

              aaaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTg                                                                           <  2:3865338/93‑1 (MQ=255)

               aaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTgg                                                                          >  1:1919008/1‑93 (MQ=255)

               aaaCTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGGTgg                                                                          >  1:4298950/1‑93 (MQ=255)

                aaCTTCGCAGGCGGAGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGAGGGAAGCCGGTTTATGCCGTTGGc                                                                         <  2:2533799/93‑1 (MQ=255)

                  cTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCga                                                                       <  2:4631955/93‑1 (MQ=255)

                   ttCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCgag                                                                      >  2:1615261/1‑93 (MQ=255)

                      gCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAAc                                                                   <  1:3873562/93‑1 (MQ=255)

                      gCAGGCGGCGACCAACCATCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAAc                                                                   <  1:4146637/93‑1 (MQ=255)

                       cAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACt                                                                  >  1:2782046/1‑93 (MQ=255)

                         ggcggcggcCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  1:3300036/93‑1 (MQ=255)

                         ggcggcGACCACCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  2:4415734/93‑1 (MQ=255)

                         ggcggcGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAc                                                                <  1:2063228/93‑1 (MQ=255)

                          gcggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACa                                                               >  2:3591933/1‑93 (MQ=255)

                          gcggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACa                                                               <  2:1169265/93‑1 (MQ=255)

                           cggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAg                                                              <  1:609451/93‑1 (MQ=255)

                            ggcgACCAACCGTCTATGCAGTACATCGGCAAAGGTCTGAAAAAGTTTAGTGACAGAGGGAAACCGGTTTATGCCATTGGCTAGAACTACAGc                                                             >  2:2523798/1‑93 (MQ=255)

                            ggcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGc                                                             <  1:4559781/93‑1 (MQ=255)

                             gcgACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGcc                                                            <  1:3213142/93‑1 (MQ=255)

                                aCCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAgg                                                         <  2:1125356/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:2131383/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        >  2:155246/1‑93 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:1882838/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        <  1:2055281/93‑1 (MQ=255)

                                 ccAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCAGGTTTATGCCGTTGGCGAGAACTACAGCCAggg                                                        >  1:1658568/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      >  2:822651/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      >  2:3877901/1‑93 (MQ=255)

                                   aaCCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGc                                                      <  2:98048/93‑1 (MQ=255)

                                     ccGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCaa                                                    <  2:2468187/93‑1 (MQ=255)

                                       gTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAata                                                  <  1:1084096/93‑1 (MQ=255)

                                       gTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAata                                                  <  1:1398276/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 <  1:1764918/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 >  2:736056/1‑93 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAatat                                                 <  2:1376029/93‑1 (MQ=255)

                                        tCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTAAAGCCAGGGGCAatat                                                 >  2:283512/1‑93 (MQ=255)

                                          tATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAAtatta                                               <  2:546400/93‑1 (MQ=255)

                                          tATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAAtatta                                               <  1:3959066/93‑1 (MQ=255)

                                            tGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtc                                             <  1:3562913/93‑1 (MQ=255)

                                             gCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtct                                            <  1:85251/93‑1 (MQ=255)

                                             gCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtct                                            <  2:407950/93‑1 (MQ=255)

                                              cAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAtctc                                           <  1:3220717/93‑1 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         >  1:1348719/1‑93 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         <  1:3628463/93‑1 (MQ=255)

                                                gTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGc                                         >  1:1106284/1‑93 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  2:1087020/93‑1 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  1:621134/93‑1 (MQ=255)

                                                 tACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGcc                                        <  1:3365611/93‑1 (MQ=255)

                                                  aCATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCa                                       <  2:1345289/93‑1 (MQ=255)

                                                  aCATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCa                                       <  2:4345901/93‑1 (MQ=255)

                                                   cATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAg                                      <  2:4552465/93‑1 (MQ=255)

                                                    aTCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGt                                     >  1:1620471/1‑93 (MQ=255)

                                                       ggCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGTGGCAATATTATCTCGCCAGTTTc                                  >  2:3393872/1‑93 (MQ=255)

                                                        gCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCg                                 >  2:2127155/1‑93 (MQ=255)

                                                           aaGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCa                              <  2:3218616/93‑1 (MQ=255)

                                                           aaGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCa                              >  1:2359857/1‑93 (MQ=255)

                                                            aGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCaa                             >  1:2093232/1‑93 (MQ=255)

                                                            aGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCaa                             >  1:574285/1‑93 (MQ=255)

                                                             gCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAAt                            >  2:1153612/1‑93 (MQ=255)

                                                              ctctGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATa                           >  1:3408005/1‑93 (MQ=255)

                                                              ctctGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATa                           <  2:2059577/93‑1 (MQ=255)

                                                                 tGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGACGTTGGCGAGAACTACAGCCACGGGCAATATTATCTCGTCAGTTTCGCCAATaaaa                        >  2:255332/1‑93 (MQ=255)

                                                                   aaaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGGAATATTATATCGCAAGTTTGGCCAATAAAAtt                      >  1:1900535/1‑93 (MQ=255)

                                                                    aaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAAttt                     >  1:2559375/1‑93 (MQ=255)

                                                                    aaGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAAttt                     <  1:3673710/93‑1 (MQ=255)

                                                                      gagTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTgg                   <  2:4527972/93‑1 (MQ=255)

                                                                        gTTTGGTGACAGCGGGAATGCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGCCAATATTATCTCGCCAGACTCGCCAATAAAATTTGGCt                 >  2:872450/1‑93 (MQ=255)

                                                                        gTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCt                 <  2:924448/93‑1 (MQ=255)

                                                                        gTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCt                 >  2:872449/1‑93 (MQ=255)

                                                                         tttCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTg                <  1:1158618/93‑1 (MQ=255)

                                                                         tttCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTg                >  2:4561195/1‑93 (MQ=255)

                                                                           tCGTGGCAACGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtc              <  2:2841101/93‑1 (MQ=255)

                                                                           tCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtc              <  1:4679467/93‑1 (MQ=255)

                                                                            cGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtct             >  1:366981/1‑93 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            <  2:1498222/93‑1 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            <  1:4283653/93‑1 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            >  2:2154747/1‑93 (MQ=255)

                                                                             gTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGtctc            >  1:3011788/1‑93 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           <  2:1599049/93‑1 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           <  2:4660233/93‑1 (MQ=255)

                                                                              tGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTcc           >  2:1895453/1‑93 (MQ=255)

                                                                                  aGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCaa       >  1:1217244/1‑93 (MQ=255)

                                                                                  aGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCaa       <  1:4607139/93‑1 (MQ=255)

                                                                                     gggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGc    <  2:1143909/93‑1 (MQ=255)

                                                                                     gggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGc    <  1:1439836/93‑1 (MQ=255)

                                                                                      ggAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCg   >  2:61658/1‑93 (MQ=255)

TGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGT  >  NC_000913/1847208‑1847387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGT  >  NC_000913/1847205‑1847390

TATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAG                                                                                              >  SRR4302544.1729132/1‑94 (MQ=60)

 ATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGC                                                                                             >  SRR4302544.123482/1‑94 (MQ=60)

  TTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCC                                                                                            <  SRR4302544.1012688/94‑1 (MQ=60)

      GATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTT                                                                                        <  SRR4302544.2235166/94‑1 (MQ=60)

      GATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTT                                                                                        <  SRR4302544.1375436/94‑1 (MQ=60)

       ATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTT                                                                                       <  SRR4302544.694715/94‑1 (MQ=60)

        TGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTA                                                                                      <  SRR4302544.1478869/94‑1 (MQ=60)

          GATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATG                                                                                    >  SRR4302544.140013/1‑94 (MQ=60)

              TGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGT                                                                                >  SRR4302544.2588555/1‑94 (MQ=60)

                 AAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGG                                                                             <  SRR4302544.672148/94‑1 (MQ=60)

                 AAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGG                                                                             >  SRR4302544.1937549/1‑94 (MQ=60)

                 AAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGG                                                                             <  SRR4302544.3606629/94‑1 (MQ=60)

                 AAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGGTGG                                                                             >  SRR4302544.4342820/1‑94 (MQ=60)

                    ACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGA                                                                          >  SRR4302544.4762372/1‑94 (MQ=60)

                     CTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAG                                                                         <  SRR4302544.2362815/94‑1 (MQ=60)

                        CGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAAC                                                                      >  SRR4302544.3815439/1‑94 (MQ=60)

                         GCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACT                                                                     <  SRR4302544.3912031/94‑1 (MQ=60)

                         GCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACT                                                                     >  SRR4302544.2808822/1‑94 (MQ=60)

                         GCAGGCGGCGACCAACCATCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACT                                                                     <  SRR4302544.4187691/94‑1 (MQ=60)

                            GGCGGCGGCCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACA                                                                  <  SRR4302544.3331468/94‑1 (MQ=60)

                            GGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACA                                                                  <  SRR4302544.2083064/94‑1 (MQ=60)

                            GGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACA                                                                  <  SRR4302544.1211063/94‑1 (MQ=60)

                              CGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGC                                                                <  SRR4302544.615437/94‑1 (MQ=60)

                               GGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCC                                                               <  SRR4302544.4605260/94‑1 (MQ=60)

                                GCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCA                                                              <  SRR4302544.3243786/94‑1 (MQ=60)

                                  GACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGG                                                            <  SRR4302544.1809710/94‑1 (MQ=60)

                                   ACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCAGGTTTATGCCGTTGGCGAGAACTACAGCCAGGG                                                           >  SRR4302544.1674905/1‑94 (MQ=60)

                                    CCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGG                                                          <  SRR4302544.1901038/94‑1 (MQ=60)

                                    CCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGG                                                          <  SRR4302544.2075058/94‑1 (MQ=60)

                                    CCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGG                                                          <  SRR4302544.2151789/94‑1 (MQ=60)

                                          GTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATAT                                                    <  SRR4302544.1094880/94‑1 (MQ=60)

                                          GTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATAT                                                    <  SRR4302544.1412214/94‑1 (MQ=60)

                                           TCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATT                                                   <  SRR4302544.1782187/94‑1 (MQ=60)

                                             TATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTAT                                                 <  SRR4302544.3998436/94‑1 (MQ=60)

                                               TGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCT                                               >  SRR4302544.1665172/1‑94 (MQ=60)

                                               TGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCT                                               <  SRR4302544.3597072/94‑1 (MQ=60)

                                                GCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTC                                              <  SRR4302544.86058/94‑1 (MQ=60)

                                                 CAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCG                                             <  SRR4302544.3251452/94‑1 (MQ=60)

                                                  AGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGTCGAGAACTACAGCCAGGGGCAATATTATCTCGC                                            >  SRR4302544.4611625/1‑94 (MQ=60)

                                                  AGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGC                                            >  SRR4302544.1117309/1‑94 (MQ=60)

                                                  AGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGC                                            >  SRR4302544.1362087/1‑94 (MQ=60)

                                                   GTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCC                                           <  SRR4302544.3664613/94‑1 (MQ=60)

                                                    TACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCA                                          <  SRR4302544.3397735/94‑1 (MQ=60)

                                                    TACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCA                                          <  SRR4302544.627228/94‑1 (MQ=60)

                                                      CATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGT                                        >  SRR4302544.1636432/1‑94 (MQ=60)

                                                         CGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTC                                     >  SRR4302544.3153067/1‑94 (MQ=60)

                                                         CGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTC                                     >  SRR4302544.2684136/1‑94 (MQ=60)

                                                            CAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGCTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCC                                  >  SRR4302544.138746/1‑94 (MQ=60)

                                                             AAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCA                                 >  SRR4302544.2382199/1‑94 (MQ=60)

                                                              AAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAA                                >  SRR4302544.2113382/1‑94 (MQ=60)

                                                              AAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAA                                >  SRR4302544.579899/1‑94 (MQ=60)

                                                              AAGCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAA                                >  SRR4302544.2113380/1‑94 (MQ=60)

                                                                GCTCTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATA                              >  SRR4302544.3440592/1‑94 (MQ=60)

                                                                   CTGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAA                           >  SRR4302544.4269171/1‑94 (MQ=60)

                                                                    TGAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAAT                          >  SRR4302544.3377162/1‑94 (MQ=60)

                                                                     GAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGGAATATTATATCGCAAGTTTGGCCAATAAAATT                         >  SRR4302544.1918897/1‑94 (MQ=60)

                                                                     GAAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATT                         >  SRR4302544.2042743/1‑94 (MQ=60)

                                                                      AAAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTT                        >  SRR4302544.2583955/1‑94 (MQ=60)

                                                                       AAGAGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTG                       <  SRR4302544.3710350/94‑1 (MQ=60)

                                                                          AGTTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCT                    >  SRR4302544.3485567/1‑94 (MQ=60)

                                                                            TTTCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGT                  <  SRR4302544.1170151/94‑1 (MQ=60)

                                                                              TCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCT                >  SRR4302544.370734/1‑94 (MQ=60)

                                                                              TCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCT                <  SRR4302544.4725729/94‑1 (MQ=60)

                                                                              TCGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCT                <  SRR4302544.885149/94‑1 (MQ=60)

                                                                               CGTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTC               >  SRR4302544.3040653/1‑94 (MQ=60)

                                                                                GTGACAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCC              <  SRR4302544.4327409/94‑1 (MQ=60)

                                                                                    CAGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAA          >  SRR4302544.1229288/1‑94 (MQ=60)

                                                                                     AGCGGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAG         <  SRR4302544.4652907/94‑1 (MQ=60)

                                                                                        GGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCG      <  SRR4302544.141637/94‑1 (MQ=60)

                                                                                        GGGAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCG      <  SRR4302544.1454165/94‑1 (MQ=60)

                                                                                          GAAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTG    <  SRR4302544.3702828/94‑1 (MQ=60)

                                                                                           AAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGG   >  SRR4302544.1846487/1‑94 (MQ=60)

                                                                                           AAGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGG   >  SRR4302544.869123/1‑94 (MQ=60)

                                                                                            AGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCTAATAAAATTTGGCTGTCTCCGCAAGGCGTGGT  >  SRR4302544.3449023/1‑94 (MQ=60)

                                                                                            AGCCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGT  >  SRR4302544.4629231/1‑94 (MQ=60)

TATTGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACATCGGCAAAGCTCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATATTATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGT  >  NC_000913/1847205‑1847390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`