Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,877,795 T→C S60G (AGT→GGT)  yeaR ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,877,7950TC98.6% 455.2 / ‑2.8 143S60G (AGT→GGT) yeaRhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (64/77);  total (65/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 155 total aligned reads displayed.

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884
                                                                                          |                                                                                          
cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                          <  1:105932/93‑1 (MQ=255)
 agtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgtg                                                                                         >  2:269338/1‑93 (MQ=255)
  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                        <  2:2132137/93‑1 (MQ=255)
   tcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGtt                                                                                       >  1:447919/1‑93 (MQ=255)
       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                   <  2:1236512/93‑1 (MQ=255)
       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                   <  1:7947/93‑1 (MQ=255)
        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:652177/93‑1 (MQ=255)
        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:185506/93‑1 (MQ=255)
        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                  <  1:2751529/93‑1 (MQ=255)
          ttCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGc                                                                                <  2:1858569/93‑1 (MQ=255)
            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                              <  2:2952525/93‑1 (MQ=255)
             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                             >  1:552541/1‑93 (MQ=255)
             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                             <  1:108367/93‑1 (MQ=255)
              aTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAg                                                                            >  2:1607342/1‑93 (MQ=255)
               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           <  1:4498662/93‑1 (MQ=255)
               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           <  2:1236841/93‑1 (MQ=255)
               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                           >  1:13478/1‑93 (MQ=255)
                 ttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                         >  1:2055421/1‑93 (MQ=255)
                 ttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                         <  1:865703/93‑1 (MQ=255)
                   gtgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgag                                                                       >  2:968797/1‑93 (MQ=255)
                   gtgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgag                                                                       <  1:1424826/93‑1 (MQ=255)
                    tgCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCgaga                                                                      <  1:3249270/93‑1 (MQ=255)
                        ccTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCCCCGTGTTCATCAGCGTAGCCGAGATAtt                                                                  <  1:3575567/92‑1 (MQ=255)
                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                 <  1:770638/93‑1 (MQ=255)
                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                 >  2:2191732/1‑93 (MQ=255)
                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                                <  1:304004/93‑1 (MQ=255)
                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                                >  1:4616644/1‑93 (MQ=255)
                             tCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAcc                                                             <  2:3799688/93‑1 (MQ=255)
                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                           >  2:3603939/1‑93 (MQ=255)
                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                           >  2:4447046/1‑93 (MQ=255)
                                ggAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcc                                                          >  1:2177290/1‑93 (MQ=255)
                                  aGGGAACACCGCAAACTGCCCCGCTTCGATAATGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcccc                                                        <  1:3222618/93‑1 (MQ=255)
                                   gggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCa                                                       <  1:3353699/93‑1 (MQ=255)
                                    ggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCAt                                                      >  2:4631422/1‑93 (MQ=255)
                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  1:1974876/1‑93 (MQ=255)
                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  1:2968795/1‑93 (MQ=255)
                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   >  2:3276472/1‑93 (MQ=255)
                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGCCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                   <  2:3179125/93‑1 (MQ=255)
                                        cacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCAt                                                  <  2:1568247/93‑1 (MQ=255)
                                        cacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCAt                                                  <  1:4520700/93‑1 (MQ=255)
                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                                <  1:149255/93‑1 (MQ=255)
                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                               <  1:2842856/93‑1 (MQ=255)
                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                               <  1:2842855/93‑1 (MQ=255)
                                            gCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACg                                              >  2:2121458/1‑93 (MQ=255)
                                               aaCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaa                                           >  2:616637/1‑93 (MQ=255)
                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGTATAACGGaaa                                          <  1:4194022/93‑1 (MQ=255)
                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaaa                                          <  1:2042245/93‑1 (MQ=255)
                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                         >  1:2415469/1‑93 (MQ=255)
                                                  tGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAgg                                        >  1:1445810/1‑93 (MQ=255)
                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                       >  1:2679030/1‑93 (MQ=255)
                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                       >  1:3640067/1‑93 (MQ=255)
                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                      >  2:2572105/1‑93 (MQ=255)
                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                      >  1:1071967/1‑93 (MQ=255)
                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCACGCATAACGGAAAGGCg                                      >  1:4223406/1‑93 (MQ=255)
                                                     cccGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  1:4377884/93‑1 (MQ=255)
                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  2:3210429/93‑1 (MQ=255)
                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  1:1334821/93‑1 (MQ=255)
                                                     cccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGt                                     <  2:1447609/93‑1 (MQ=255)
                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    <  2:1049280/93‑1 (MQ=255)
                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    >  1:892842/1‑93 (MQ=255)
                                                      ccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTg                                    >  2:4450576/1‑93 (MQ=255)
                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGtcg                                   >  2:2559014/1‑91 (MQ=255)
                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                   >  2:3738076/1‑93 (MQ=255)
                                                        gCTTGGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  <  1:3988099/93‑1 (MQ=255)
                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3312563/1‑93 (MQ=255)
                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3943328/1‑93 (MQ=255)
                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                  >  1:3739579/1‑93 (MQ=255)
                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                >  1:2425629/1‑93 (MQ=255)
                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                <  2:2988487/93‑1 (MQ=255)
                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                                <  2:614992/93‑1 (MQ=255)
                                                            cGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGaaaa                              >  1:994160/1‑93 (MQ=255)
                                                             gATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑c                            >  2:3957792/1‑93 (MQ=255)
                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑cc                           >  1:295213/1‑93 (MQ=255)
                                                               tAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCACCATGCATAACGGAAAGGCGTGGGTAAA‑ccc                          >  2:4596371/1‑93 (MQ=255)
                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        <  1:413973/93‑1 (MQ=255)
                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        >  1:1959561/1‑93 (MQ=255)
                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        >  2:4271882/1‑93 (MQ=255)
                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑ccccc                        <  1:4380235/93‑1 (MQ=255)
                                                                  ggATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCg                       >  1:3527132/1‑93 (MQ=255)
                                                                  ggATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCg                       <  1:70332/93‑1 (MQ=255)
                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCgg                      <  2:1636299/93‑1 (MQ=255)
                                                                       aCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGgcgc                  <  2:3878798/93‑1 (MQ=255)
                                                                          tGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGtt               >  1:289854/1‑93 (MQ=255)
                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTcc             >  1:128070/1‑93 (MQ=255)
                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCCGGGCGCGTTcc             <  2:2693358/93‑1 (MQ=255)
                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCtt           <  1:1038184/93‑1 (MQ=255)
                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCtt           >  2:3983532/1‑93 (MQ=255)
                                                                               aGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCttt          <  2:4222681/93‑1 (MQ=255)
                                                                               aGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCttt          >  1:4687849/1‑93 (MQ=255)
                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTa         >  2:343386/1‑93 (MQ=255)
                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTa         <  1:979216/93‑1 (MQ=255)
                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  2:1947928/93‑1 (MQ=255)
                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  1:3848271/93‑1 (MQ=255)
                                                                                 gCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTAt        <  1:519723/93‑1 (MQ=255)
                                                                                   tctGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCa      >  2:3680604/1‑93 (MQ=255)
                                                                                    ctGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCaa     >  1:2572490/1‑93 (MQ=255)
                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGa   <  2:3882372/93‑1 (MQ=255)
                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGa   >  1:2294653/1‑93 (MQ=255)
                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGGGTTTCTTTATCAAGAt  >  2:4241756/1‑93 (MQ=255)
                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAt  <  2:3837838/93‑1 (MQ=255)
                                                                                          |                                                                                          
CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAA‑CCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACG  >  NC_000913/1877702‑1877888
                                                                                             |                                                                                             
CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACC                                                                                               <  SRR4302544.3642779/94‑1 (MQ=60)
CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACC                                                                                               >  SRR4302544.3805797/1‑94 (MQ=60)
  TCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGT                                                                                             >  SRR4302544.3202818/1‑94 (MQ=60)
  TCAGTCATGGCTTCAATGTTGTGACACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGT                                                                                             >  SRR4302544.1094520/1‑94 (MQ=60)
   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302544.4189591/94‑1 (MQ=60)
   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302544.106904/94‑1 (MQ=60)
   CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTG                                                                                            <  SRR4302544.106906/94‑1 (MQ=60)
    AGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGT                                                                                           <  SRR4302544.4342885/94‑1 (MQ=60)
     GTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTT                                                                                          >  SRR4302544.452376/1‑94 (MQ=60)
          GGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCA                                                                                     <  SRR4302544.8019/94‑1 (MQ=60)
           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302544.84127/94‑1 (MQ=60)
           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302544.658634/94‑1 (MQ=60)
           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302544.2778028/94‑1 (MQ=60)
           GCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAG                                                                                    <  SRR4302544.187349/94‑1 (MQ=60)
               CAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTA                                                                                >  SRR4302544.557946/1‑94 (MQ=60)
                AATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAG                                                                               <  SRR4302544.109358/94‑1 (MQ=60)
                 ATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGC                                                                              >  SRR4302544.13607/1‑94 (MQ=60)
                 ATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGC                                                                              >  SRR4302544.13606/1‑94 (MQ=60)
                  TGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCC                                                                             <  SRR4302544.4543807/94‑1 (MQ=60)
                   GTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCG                                                                            <  SRR4302544.1945313/94‑1 (MQ=60)
                   GTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCG                                                                            >  SRR4302544.2075199/1‑94 (MQ=60)
                    TTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGA                                                                           <  SRR4302544.874267/94‑1 (MQ=60)
                    TTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGA                                                                           >  SRR4302544.3352954/1‑94 (MQ=60)
                      GTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGA                                                                         <  SRR4302544.1439006/94‑1 (MQ=60)
                       TGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGAT                                                                        <  SRR4302544.3314823/94‑1 (MQ=60)
                       TGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGAT                                                                        <  SRR4302544.3280362/94‑1 (MQ=60)
                        GCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATA                                                                       >  SRR4302544.2992674/1‑94 (MQ=60)
                         CCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAT                                                                      <  SRR4302544.52624/94‑1 (MQ=60)
                         CCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAT                                                                      <  SRR4302544.409671/94‑1 (MQ=60)
                           CCTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCCCCGTGTTCATCAGCGTAGCCGAGATATTT                                                                    <  SRR4302544.3609863/94‑1 (MQ=60)
                            CTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   >  SRR4302544.4662473/1‑94 (MQ=60)
                            CTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTG                                                                   <  SRR4302544.778137/94‑1 (MQ=60)
                             TTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGA                                                                  <  SRR4302544.307157/94‑1 (MQ=60)
                                TCTGGAGGGAACCCCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCG                                                               <  SRR4302544.1577192/94‑1 (MQ=60)
                                TCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCG                                                               >  SRR4302544.4490696/1‑94 (MQ=60)
                                  TGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCC                                                             >  SRR4302544.2197914/1‑94 (MQ=60)
                                   GGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCC                                                            >  SRR4302544.2613747/1‑94 (MQ=60)
                                     AGGGAACACCGCAAACTGCCCCGCTTCGATAATGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCA                                                          <  SRR4302544.3253385/94‑1 (MQ=60)
                                      GGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCAT                                                         <  SRR4302544.3385703/94‑1 (MQ=60)
                                         AACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCA                                                      >  SRR4302544.1994064/1‑94 (MQ=60)
                                         AACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCA                                                      >  SRR4302544.2997266/1‑94 (MQ=60)
                                         AACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCA                                                      >  SRR4302544.1994065/1‑94 (MQ=60)
                                           CACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATA                                                    <  SRR4302544.4565960/94‑1 (MQ=60)
                                             CCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAC                                                  <  SRR4302544.150683/94‑1 (MQ=60)
                                              CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACG                                                 <  SRR4302544.2870295/94‑1 (MQ=60)
                                              CGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACG                                                 <  SRR4302544.2870294/94‑1 (MQ=60)
                                                  AACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAA                                             >  SRR4302544.1459685/1‑94 (MQ=60)
                                                   ACTGCCCCGCTTCGATAAGGATCCCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAG                                            <  SRR4302544.3605342/94‑1 (MQ=60)
                                                   ACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGTATAACGGAAAG                                            <  SRR4302544.4235581/94‑1 (MQ=60)
                                                   ACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAG                                            <  SRR4302544.2061946/94‑1 (MQ=60)
                                                   ACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAG                                            >  SRR4302544.2438457/1‑94 (MQ=60)
                                                    CTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGG                                           >  SRR4302544.1460188/1‑94 (MQ=60)
                                                     TGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGC                                          >  SRR4302544.3676381/1‑94 (MQ=60)
                                                     TGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGC                                          >  SRR4302544.2704703/1‑94 (MQ=60)
                                                      GCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCG                                         >  SRR4302544.1082622/1‑94 (MQ=60)
                                                      GCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCACGCATAACGGAAAGGCG                                         >  SRR4302544.4266297/1‑94 (MQ=60)
                                                        CCCGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       <  SRR4302544.4422248/94‑1 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302544.4687284/1‑94 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       <  SRR4302544.1347976/94‑1 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       <  SRR4302544.577038/94‑1 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302544.982344/1‑94 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTG                                       >  SRR4302544.901679/1‑94 (MQ=60)
                                                        CCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGAAATTTGACCGCCCCATGCATAACGGAAAGGAGTG                                       >  SRR4302544.3757337/1‑94 (MQ=60)
                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     >  SRR4302544.3344105/1‑94 (MQ=60)
                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     >  SRR4302544.3776746/1‑94 (MQ=60)
                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     <  SRR4302544.772597/94‑1 (MQ=60)
                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     >  SRR4302544.3982507/1‑94 (MQ=60)
                                                          CGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGG                                     <  SRR4302544.2584261/94‑1 (MQ=60)
                                                           GCTTGGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGT                                    <  SRR4302544.4027799/94‑1 (MQ=60)
                                                            CTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTA                                   >  SRR4302544.2448718/1‑94 (MQ=60)
                                                              TCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGAAAA                                 >  SRR4302544.1003991/1‑94 (MQ=60)
                                                                GATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACC                               >  SRR4302544.298262/1‑94 (MQ=60)
                                                                GATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACC                               <  SRR4302544.2910671/94‑1 (MQ=60)
                                                                   AAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCC                            >  SRR4302544.1978573/1‑94 (MQ=60)
                                                                    AGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCG                           <  SRR4302544.418198/94‑1 (MQ=60)
                                                                    AGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCG                           >  SRR4302544.3560839/1‑94 (MQ=60)
                                                                    AGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCG                           <  SRR4302544.4424609/94‑1 (MQ=60)
                                                                     GGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGG                          <  SRR4302544.71018/94‑1 (MQ=60)
                                                                         CACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGC                      >  SRR4302544.455911/1‑94 (MQ=60)
                                                                           CCTGATCAGGCTCTGCACCGTGTTCATCATCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGAAAACCCCCGGGCGCGT                    >  SRR4302544.2338838/1‑94 (MQ=60)
                                                                            CTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTT                   >  SRR4302544.292855/1‑94 (MQ=60)
                                                                              GATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCC                 >  SRR4302544.129250/1‑94 (MQ=60)
                                                                                 CAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTT              <  SRR4302544.1048500/94‑1 (MQ=60)
                                                                                 CAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTT              >  SRR4302544.4734177/1‑94 (MQ=60)
                                                                                   GGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTAT            <  SRR4302544.988809/94‑1 (MQ=60)
                                                                                    GCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATC           <  SRR4302544.524854/94‑1 (MQ=60)
                                                                                    GCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATC           <  SRR4302544.3886463/94‑1 (MQ=60)
                                                                                     CTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCA          <  SRR4302544.2831710/94‑1 (MQ=60)
                                                                                     CTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCA          >  SRR4302544.2145548/1‑94 (MQ=60)
                                                                                     CTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCA          >  SRR4302544.1212389/1‑94 (MQ=60)
                                                                                      TCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAA         >  SRR4302544.2597206/1‑94 (MQ=60)
                                                                                       CTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAG        <  SRR4302544.3933373/94‑1 (MQ=60)
                                                                                        TGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGA       >  SRR4302544.2316284/1‑94 (MQ=60)
                                                                                            CCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGAC   <  SRR4302544.3025338/94‑1 (MQ=60)
                                                                                            CCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGAC   <  SRR4302544.2095356/94‑1 (MQ=60)
                                                                                             CGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACG  >  SRR4302544.3188325/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTCAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGATGACG  >  NC_000913/1877702‑1877888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑