Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,890,225 C→T R345R (CGG→CGA)  b1808 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,890,2250CT99.1% 403.2 / ‑4.9 116R345R (CGG→CGA) b1808hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (57/58);  minor base A (0/1);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 120 total aligned reads displayed.

GAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  >  NC_000913/1890135‑1890317
                                                                                          |                                                                                            
gAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCg                                                                                            >  2:233427/1‑93 (MQ=255)
 aGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCgt                                                                                           <  1:1665639/93‑1 (MQ=255)
   cggcgTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTAGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCgtgt                                                                                         <  1:4084205/93‑1 (MQ=255)
    ggcgTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGtgtg                                                                                        <  2:852377/93‑1 (MQ=255)
       gTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGa                                                                                     >  2:947032/1‑93 (MQ=255)
        tGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGAt                                                                                    >  1:2173876/1‑93 (MQ=255)
         gagaGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATa                                                                                   <  2:1649237/93‑1 (MQ=255)
          agagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATAc                                                                                  <  1:2146308/93‑1 (MQ=255)
           gagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACa                                                                                 <  2:4327275/93‑1 (MQ=255)
            agCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACaa                                                                                <  1:2081743/93‑1 (MQ=255)
            agCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACaa                                                                                >  2:1236276/1‑93 (MQ=255)
             gCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAAc                                                                               <  1:516693/93‑1 (MQ=255)
               cAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGt                                                                             <  1:21508/93‑1 (MQ=255)
               cAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGt                                                                             >  1:4029300/1‑93 (MQ=255)
               cAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGt                                                                             <  2:2302264/93‑1 (MQ=255)
               cAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGt                                                                             <  2:2999365/93‑1 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                            >  1:737301/1‑93 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                            >  2:1374384/1‑93 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                            <  1:368577/93‑1 (MQ=255)
                 gagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTgg                                                                           >  2:2072987/1‑93 (MQ=255)
                  agTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgc                                                                          <  1:161756/93‑1 (MQ=255)
                  agTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgc                                                                          >  1:1820566/1‑93 (MQ=255)
                   gTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcg                                                                         >  1:2811179/1‑93 (MQ=255)
                   gTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcg                                                                         >  1:2518026/1‑93 (MQ=255)
                    tAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgc                                                                        <  2:765787/93‑1 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                      >  2:3890443/1‑93 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                      <  1:2782357/93‑1 (MQ=255)
                       aaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCt                                                                     <  2:1471240/93‑1 (MQ=255)
                           gcgcGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCaaa                                                                 <  2:2687390/93‑1 (MQ=255)
                           gcgcGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCaaa                                                                 <  1:44128/93‑1 (MQ=255)
                           gcgcGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCaaa                                                                 <  2:3603206/93‑1 (MQ=255)
                             gcgAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATg                                                               <  1:3485864/93‑1 (MQ=255)
                               gAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGcc                                                             >  1:2367160/1‑93 (MQ=255)
                               gAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGcc                                                             >  2:22250/1‑93 (MQ=255)
                               gAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGcc                                                             >  2:1981635/1‑93 (MQ=255)
                               gAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGcc                                                             >  1:1341601/1‑93 (MQ=255)
                                  gTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCa                                                          <  2:1239909/93‑1 (MQ=255)
                                   tGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCAt                                                         <  1:3069948/93‑1 (MQ=255)
                                   tGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCAt                                                         >  1:2578012/1‑93 (MQ=255)
                                     cATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATcc                                                       <  1:3778352/93‑1 (MQ=255)
                                     cATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATcc                                                       >  2:2533613/1‑93 (MQ=255)
                                       ttCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAg                                                     >  2:4082353/1‑93 (MQ=255)
                                       ttCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAg                                                     <  1:1348652/93‑1 (MQ=255)
                                       ttCGTACCAGTAGCTGTAGCCCGGCTGATTGATATCTTTTAGCCGCTTCAGTTGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCac                                                     >  1:3792389/1‑92 (MQ=255)
                                           tACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAg                                                 >  1:568836/1‑93 (MQ=255)
                                            aCCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAgg                                                <  1:2057092/93‑1 (MQ=255)
                                            aCCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAgg                                                >  1:2640919/1‑93 (MQ=255)
                                            aCCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAgg                                                >  2:2994697/1‑93 (MQ=255)
                                             ccAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGc                                               <  1:111024/93‑1 (MQ=255)
                                             ccAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGc                                               <  1:2182340/93‑1 (MQ=255)
                                              cAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCg                                              >  2:757/1‑93 (MQ=255)
                                               aGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCgg                                             <  2:4375762/93‑1 (MQ=255)
                                               aGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCgg                                             >  2:195206/1‑93 (MQ=255)
                                                 tAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGaa                                           <  1:864871/93‑1 (MQ=255)
                                                 tAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGaa                                           >  1:689791/1‑93 (MQ=255)
                                                    cTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGc                                        <  1:2491709/93‑1 (MQ=255)
                                                     tGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGcc                                       >  2:2692617/1‑93 (MQ=255)
                                                      gTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGccc                                      >  1:4657793/1‑93 (MQ=255)
                                                       tAGCCCGGCTGATTGATCTCTTGTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGcccc                                     <  2:2463512/93‑1 (MQ=255)
                                                           ccGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTg                                 >  2:5420/1‑93 (MQ=255)
                                                           ccGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTg                                 >  2:5419/1‑93 (MQ=255)
                                                            cGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGa                                <  1:838361/93‑1 (MQ=255)
                                                            cGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGa                                <  1:3378801/93‑1 (MQ=255)
                                                              gCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACa                              >  1:445793/1‑93 (MQ=255)
                                                               cTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAg                             >  2:2019366/1‑93 (MQ=255)
                                                               cTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAg                             <  1:126603/93‑1 (MQ=255)
                                                                 gattgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGtt                           >  1:3405587/1‑93 (MQ=255)
                                                                 gattgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGtt                           <  1:3108985/93‑1 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                          >  1:1076534/1‑93 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                          <  1:2776942/93‑1 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                          >  2:302671/1‑93 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                          >  2:302670/1‑93 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                         >  2:1115418/1‑93 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                         >  1:4319947/1‑93 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTGCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                         <  2:2253908/93‑1 (MQ=255)
                                                                    tgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCg                        >  1:3552473/1‑93 (MQ=255)
                                                                     gatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGc                       >  1:4127838/1‑93 (MQ=255)
                                                                      atCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGcc                      >  2:1446677/1‑93 (MQ=255)
                                                                       tctcTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCa                     >  2:4144359/1‑93 (MQ=255)
                                                                        ctctTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCAc                    <  2:2494772/93‑1 (MQ=255)
                                                                         tctTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCACGGCGGACGGCCCCAGTGACAGTTTCGCCACg                   >  1:4139459/1‑93 (MQ=255)
                                                                         tctTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACg                   >  2:931221/1‑93 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACACCGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc                 <  2:2506068/93‑1 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc                 <  1:341639/93‑1 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc                 >  1:4393521/1‑93 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc                 >  1:2273215/1‑93 (MQ=255)
                                                                             ttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCAt               <  2:4527825/93‑1 (MQ=255)
                                                                              tAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATa              <  2:2985985/93‑1 (MQ=255)
                                                                               aGCCGCTTCGGTCGTGTGCGATACAACGTGGCGCGCTCGAATGCCGCATCCAGCAGGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATaa             <  2:4212397/93‑1 (MQ=255)
                                                                               aGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATaa             <  2:1085196/93‑1 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc            <  1:4060516/93‑1 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa         <  2:4661048/93‑1 (MQ=255)
                                                                                    cTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAg        >  2:3617144/1‑93 (MQ=255)
                                                                                    cTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAg        <  2:3268861/93‑1 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGt       <  2:112212/93‑1 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGt       >  1:69340/1‑93 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGt       >  1:3349201/1‑93 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGGGACAGTTTCGCCACGTCATAACAAAGt       >  1:3349200/1‑93 (MQ=255)
                                                                                      tCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGtt      >  2:346824/1‑93 (MQ=255)
                                                                                          ccGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAg  >  2:4328604/1‑93 (MQ=255)
                                                                                          |                                                                                            
GAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  >  NC_000913/1890135‑1890317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGG  >  NC_000913/1890132‑1890318
                                                                                             |                                                                                             
GCTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGT                                                                                               <  SRR4302544.4606975/94‑1 (MQ=60)
 CTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTC                                                                                              >  SRR4302544.2371178/1‑94 (MQ=60)
  TGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTTCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCG                                                                                             <  SRR4302544.2550839/94‑1 (MQ=60)
  TGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCG                                                                                             <  SRR4302544.725077/94‑1 (MQ=60)
    AGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTG                                                                                           <  SRR4302544.1682045/94‑1 (MQ=60)
     GCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGT                                                                                          <  SRR4302544.346707/94‑1 (MQ=60)
      CGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTAGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTG                                                                                         <  SRR4302544.4124829/94‑1 (MQ=60)
          GTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGAT                                                                                     >  SRR4302544.2194477/1‑94 (MQ=60)
             AGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACA                                                                                  <  SRR4302544.2341223/94‑1 (MQ=60)
             AGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACA                                                                                  <  SRR4302544.2166761/94‑1 (MQ=60)
              GAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAA                                                                                 >  SRR4302544.4762816/1‑94 (MQ=60)
               AGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAAC                                                                                <  SRR4302544.2101760/94‑1 (MQ=60)
                GCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACG                                                                               <  SRR4302544.521804/94‑1 (MQ=60)
                 CCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGT                                                                              >  SRR4302544.4069433/1‑94 (MQ=60)
                  CAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTG                                                                             >  SRR4302544.744510/1‑94 (MQ=60)
                  CAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTG                                                                             <  SRR4302544.21721/94‑1 (MQ=60)
                   AGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGG                                                                            <  SRR4302544.372343/94‑1 (MQ=60)
                    GAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGC                                                                           >  SRR4302544.1838320/1‑94 (MQ=60)
                     AGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCG                                                                          <  SRR4302544.163333/94‑1 (MQ=60)
                     AGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCG                                                                          >  SRR4302544.2542208/1‑94 (MQ=60)
                     AGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCG                                                                          >  SRR4302544.2838307/1‑94 (MQ=60)
                         AAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCT                                                                      <  SRR4302544.2809134/94‑1 (MQ=60)
                              GCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAAT                                                                 <  SRR4302544.44562/94‑1 (MQ=60)
                                GCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGC                                                               <  SRR4302544.3519082/94‑1 (MQ=60)
                                 CGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCC                                                              >  SRR4302544.2389558/1‑94 (MQ=60)
                                 CGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCC                                                              >  SRR4302544.1354865/1‑94 (MQ=60)
                                     GTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCAT                                                          >  SRR4302544.2602775/1‑94 (MQ=60)
                                      TGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATC                                                         <  SRR4302544.3099203/94‑1 (MQ=60)
                                      TGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATC                                                         <  SRR4302544.4318889/94‑1 (MQ=60)
                                        CATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCA                                                       <  SRR4302544.3815783/94‑1 (MQ=60)
                                         ATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATATCTTTTAGCCGCTTCAGTTGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAC                                                      >  SRR4302544.3829938/1‑94 (MQ=60)
                                          TTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGC                                                     <  SRR4302544.1362019/94‑1 (MQ=60)
                                             GTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAG                                                  >  SRR4302544.574392/1‑94 (MQ=60)
                                              TACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGG                                                 >  SRR4302544.2666239/1‑94 (MQ=60)
                                               ACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGC                                                <  SRR4302544.2076882/94‑1 (MQ=60)
                                                CCAGTAGCTGTAGCTCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCG                                               <  SRR4302544.3344471/94‑1 (MQ=60)
                                                CCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCG                                               <  SRR4302544.112041/94‑1 (MQ=60)
                                                CCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCG                                               <  SRR4302544.2202997/94‑1 (MQ=60)
                                                   GTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAA                                            >  SRR4302544.696669/1‑94 (MQ=60)
                                                    TAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAAC                                           <  SRR4302544.873429/94‑1 (MQ=60)
                                                     AGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACG                                          <  SRR4302544.2112666/94‑1 (MQ=60)
                                                       CTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCC                                        <  SRR4302544.2515570/94‑1 (MQ=60)
                                                        TGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCC                                       >  SRR4302544.4703876/1‑94 (MQ=60)
                                                             CCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTG                                  >  SRR4302544.2673261/1‑94 (MQ=60)
                                                               CGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGAC                                <  SRR4302544.846628/94‑1 (MQ=60)
                                                               CGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGAC                                <  SRR4302544.3411052/94‑1 (MQ=60)
                                                                GGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACA                               >  SRR4302544.450241/1‑94 (MQ=60)
                                                                  CTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGT                             <  SRR4302544.127769/94‑1 (MQ=60)
                                                                   TGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTT                            >  SRR4302544.3438145/1‑94 (MQ=60)
                                                                    GATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTT                           >  SRR4302544.1087237/1‑94 (MQ=60)
                                                                    GATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTT                           <  SRR4302544.3138572/94‑1 (MQ=60)
                                                                     ATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTC                          <  SRR4302544.2803653/94‑1 (MQ=60)
                                                                     ATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTC                          >  SRR4302544.4363952/1‑94 (MQ=60)
                                                                      TTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCG                         >  SRR4302544.3586500/1‑94 (MQ=60)
                                                                       TGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGC                        >  SRR4302544.4168771/1‑94 (MQ=60)
                                                                        GATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCC                       >  SRR4302544.3953428/1‑94 (MQ=60)
                                                                           CTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCACGGCGGACGGCCCCAGTGACAGTTTCGCCACG                    >  SRR4302544.4180444/1‑94 (MQ=60)
                                                                             CTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTC                  >  SRR4302544.2294616/1‑94 (MQ=60)
                                                                             CTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTC                  >  SRR4302544.4437957/1‑94 (MQ=60)
                                                                              TTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCA                 <  SRR4302544.345173/94‑1 (MQ=60)
                                                                                TTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATA               <  SRR4302544.1627122/94‑1 (MQ=60)
                                                                                   GCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACA            <  SRR4302544.4100954/94‑1 (MQ=60)
                                                                                     CGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAA          >  SRR4302544.775686/1‑94 (MQ=60)
                                                                                       CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGT        >  SRR4302544.70022/1‑94 (MQ=60)
                                                                                       CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGT        >  SRR4302544.3381158/1‑94 (MQ=60)
                                                                                       CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGGGACAGTTTCGCCACGTCATAACAAAGT        >  SRR4302544.3381157/1‑94 (MQ=60)
                                                                                           AGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCA    >  SRR4302544.3175819/1‑94 (MQ=60)
                                                                                           AGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCA    <  SRR4302544.1995477/94‑1 (MQ=60)
                                                                                           AGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCA    <  SRR4302544.2994564/94‑1 (MQ=60)
                                                                                           AGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCA    >  SRR4302544.3175818/1‑94 (MQ=60)
                                                                                            GTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG   <  SRR4302544.2389209/94‑1 (MQ=60)
                                                                                            GTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG   <  SRR4302544.4694377/94‑1 (MQ=60)
                                                                                             TCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGG  <  SRR4302544.4636460/94‑1 (MQ=60)
                                                                                             TCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGG  <  SRR4302544.2022118/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGG  >  NC_000913/1890132‑1890318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑