Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,958,588 T→C M168T (ATG→ACG)  argS → arginine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,958,5880TC98.6% 455.9 / ‑2.6 145M168T (ATG→ACG) argSarginine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (59/84);  total (60/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

Only 100 of 148 total aligned reads displayed.

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAA  >  NC_000913/1958498‑1958676
                                                                                          |                                                                                        
 tGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCt                                                                                       <  2:4353580/93‑1 (MQ=255)
  ggTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCAGTACGCTg                                                                                      <  1:788350/93‑1 (MQ=255)
   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                     >  1:3284053/1‑93 (MQ=255)
   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                     >  2:365558/1‑93 (MQ=255)
    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                    <  2:2112038/93‑1 (MQ=255)
    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                    <  2:2868512/93‑1 (MQ=255)
     gACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTAAGTTCGGTACGCTGAtt                                                                                   <  1:1326121/93‑1 (MQ=255)
        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                                <  1:3999838/93‑1 (MQ=255)
         cagcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAt                                                                               >  1:2934246/1‑93 (MQ=255)
           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                             >  1:3699065/1‑93 (MQ=255)
           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                             >  1:2118845/1‑93 (MQ=255)
            cagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGc                                                                            <  2:2157005/93‑1 (MQ=255)
            cagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGc                                                                            <  1:1756269/93‑1 (MQ=255)
              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          <  2:4278688/93‑1 (MQ=255)
              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          >  2:2280570/1‑93 (MQ=255)
              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                          >  2:580986/1‑93 (MQ=255)
               tGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTgg                                                                         >  2:934267/1‑93 (MQ=255)
               tGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTgg                                                                         >  1:3966973/1‑93 (MQ=255)
                 cGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaa                                                                       <  1:669614/93‑1 (MQ=255)
                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                     >  1:2709446/1‑93 (MQ=255)
                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                     <  1:616775/93‑1 (MQ=255)
                    aCTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAag                                                                    >  2:2905893/1‑93 (MQ=255)
                     ctctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagc                                                                   <  2:3498224/93‑1 (MQ=255)
                     ctctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagc                                                                   >  1:3395616/1‑93 (MQ=255)
                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                  >  1:4603497/1‑93 (MQ=255)
                       ctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcag                                                                 <  1:462692/93‑1 (MQ=255)
                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  1:3179824/93‑1 (MQ=255)
                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  2:4639655/93‑1 (MQ=255)
                        tGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagc                                                                <  1:4352524/93‑1 (MQ=255)
                         ggAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagca                                                               <  1:1231012/93‑1 (MQ=255)
                          gAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagcag                                                              <  2:599891/93‑1 (MQ=255)
                            ggtCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                            >  2:138985/3‑93 (MQ=255)
                             ttCCTGGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAGGGCTGGAAAAGcagcagcag                                                           <  1:984807/93‑1 (MQ=255)
                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                        >  1:973670/1‑93 (MQ=255)
                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                        >  1:223186/1‑93 (MQ=255)
                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                       >  2:1395408/1‑93 (MQ=255)
                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                       <  2:3780792/93‑1 (MQ=255)
                                  tggTCACAAAGTGATCCGAACAAAGCGCGTCGGCGACTGCGGCACTCCGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaaa                                                      <  2:2003169/92‑1 (MQ=255)
                                  cGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaaa                                                      <  1:476319/93‑1 (MQ=255)
                                    gTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACg                                                    <  2:3104945/93‑1 (MQ=255)
                                     tCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGc                                                   <  2:1540432/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:1998155/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:1733744/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGGCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                                <  2:3306791/93‑1 (MQ=255)
                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCggg                                               >  2:25071/1‑92 (MQ=255)
                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCggg                                               >  2:3338709/1‑92 (MQ=255)
                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGt                                               <  2:2419019/93‑1 (MQ=255)
                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                              <  1:2523506/93‑1 (MQ=255)
                                              gATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                          >  1:3115895/1‑93 (MQ=255)
                                              gATTCGCGCAAACCACCTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                          <  1:3802210/93‑1 (MQ=255)
                                               aTTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                         <  1:1802623/93‑1 (MQ=255)
                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                        <  1:2958171/93‑1 (MQ=255)
                                                  cgcgCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAg                                      <  1:1863879/93‑1 (MQ=255)
                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                    >  2:3290190/1‑93 (MQ=255)
                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                    <  1:3187855/93‑1 (MQ=255)
                                                     gcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                   <  1:1020978/93‑1 (MQ=255)
                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                 >  1:316136/1‑93 (MQ=255)
                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                 >  1:4017231/1‑93 (MQ=255)
                                                        aaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggct                                >  2:4716834/1‑93 (MQ=255)
                                                          ccACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGa                              <  2:4095934/93‑1 (MQ=255)
                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             >  1:1567101/1‑93 (MQ=255)
                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             >  2:3705143/1‑93 (MQ=255)
                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                             <  2:3025013/93‑1 (MQ=255)
                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                            >  2:2048630/1‑93 (MQ=255)
                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                            <  2:3873655/93‑1 (MQ=255)
                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                           <  2:3451405/93‑1 (MQ=255)
                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                         <  2:3579464/93‑1 (MQ=255)
                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                         >  1:1441400/1‑93 (MQ=255)
                                                                cGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGa                        <  1:3725921/93‑1 (MQ=255)
                                                                 ggCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGaa                       <  1:3577670/93‑1 (MQ=255)
                                                                  gCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAg                      <  1:4540251/93‑1 (MQ=255)
                                                                  gCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAg                      >  2:1305663/1‑93 (MQ=255)
                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                    <  1:2114182/93‑1 (MQ=255)
                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                    >  1:4687402/1‑93 (MQ=255)
                                                                     aCTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGtt                   <  1:121716/93‑1 (MQ=255)
                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                 <  2:1546788/93‑1 (MQ=255)
                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                 <  2:1092610/93‑1 (MQ=255)
                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGCAAAGCAGCAGCAGGAAAACGCCGGGGAAATGGAGCTGGCTGACATTGAAGGTTACt                >  2:2475931/1‑93 (MQ=255)
                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt                <  2:436452/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  2:3133575/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:4057931/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:4373519/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:3247654/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:993888/93‑1 (MQ=255)
                                                                            cACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcg            <  2:3788269/93‑1 (MQ=255)
                                                                            cACTCAGTGCGGTACGCTGATTGCATGTCTGGAAAAGCAGCAGCAGGAAACCGCCGGTGAAATGAAGCTGGCTGACCTTGAAGGTTTCTACcg            <  1:3247653/93‑1 (MQ=255)
                                                                              cTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgcg          >  1:2397522/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAGCTTGAAGGTTTCTACCGCGa         >  2:2541635/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa         >  1:757773/1‑93 (MQ=255)
                                                                                cAGTTCGGTGCGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  2:1744716/93‑1 (MQ=255)
                                                                                cAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  1:2759733/93‑1 (MQ=255)
                                                                                cAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGAt        <  1:3814516/93‑1 (MQ=255)
                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg       >  1:1756791/1‑93 (MQ=255)
                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg       >  1:4352111/1‑93 (MQ=255)
                                                                                  gTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGc      >  2:2205235/1‑93 (MQ=255)
                                                                                  gTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGc      >  2:765494/1‑93 (MQ=255)
                                                                                    tCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGa    >  2:2064281/1‑93 (MQ=255)
                                                                                    tCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGa    >  1:1930319/1‑93 (MQ=255)
                                                                                      ggTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTTGCTGACCTTGAAGGTTTCTACCGCGATGCGaaa  >  1:2595526/1‑93 (MQ=255)
                                                                                      ggTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGaaa  >  2:4192578/1‑93 (MQ=255)
                                                                                          |                                                                                        
TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAA  >  NC_000913/1958498‑1958676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  >  NC_000913/1958496‑1958681
                                                                                            |                                                                                             
TATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACG                                                                                              <  SRR4302544.2232462/94‑1 (MQ=60)
  TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCT                                                                                            <  SRR4302544.1527778/94‑1 (MQ=60)
    GGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGA                                                                                          >  SRR4302544.3315344/1‑94 (MQ=60)
    GGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCAGTACGCTGA                                                                                          <  SRR4302544.795976/94‑1 (MQ=60)
       GACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTG                                                                                       <  SRR4302544.3194437/94‑1 (MQ=60)
       GACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTAAGTTCGGTACGCTGATTG                                                                                       <  SRR4302544.1339160/94‑1 (MQ=60)
        ACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGC                                                                                      >  SRR4302544.324413/1‑94 (MQ=60)
          GCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAT                                                                                    >  SRR4302544.2962435/1‑94 (MQ=60)
          GCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAT                                                                                    <  SRR4302544.4039649/94‑1 (MQ=60)
            AGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGG                                                                                  >  SRR4302544.2139189/1‑94 (MQ=60)
            AGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGG                                                                                  >  SRR4302544.3735895/1‑94 (MQ=60)
              CAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCT                                                                                >  SRR4302544.1329790/1‑94 (MQ=60)
              CAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCT                                                                                <  SRR4302544.1773459/94‑1 (MQ=60)
                GTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGG                                                                              >  SRR4302544.4006435/1‑94 (MQ=60)
                   CGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAA                                                                           <  SRR4302544.676230/94‑1 (MQ=60)
                    GTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAA                                                                          >  SRR4302544.2853341/1‑94 (MQ=60)
                    GTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAA                                                                          >  SRR4302544.2735507/1‑94 (MQ=60)
                     TACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAG                                                                         <  SRR4302544.622831/94‑1 (MQ=60)
                      ACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGC                                                                        >  SRR4302544.3428089/1‑94 (MQ=60)
                       CTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCA                                                                       >  SRR4302544.4649253/1‑94 (MQ=60)
                       CTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCA                                                                       <  SRR4302544.600217/94‑1 (MQ=60)
                        TCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGCCTGGAGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAG                                                                      <  SRR4302544.341740/94‑1 (MQ=60)
                         CTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGC                                                                     <  SRR4302544.467235/94‑1 (MQ=60)
                          TGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCA                                                                    <  SRR4302544.3210086/94‑1 (MQ=60)
                          TGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCA                                                                    <  SRR4302544.3119953/94‑1 (MQ=60)
                          TGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCA                                                                    <  SRR4302544.4396735/94‑1 (MQ=60)
                           GGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAG                                                                   <  SRR4302544.1243188/94‑1 (MQ=60)
                               TTCCTGGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAGGGCTGGAAAAGCAGCAGCAGG                                                               <  SRR4302544.994463/94‑1 (MQ=60)
                                 CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             >  SRR4302544.983217/1‑94 (MQ=60)
                                 CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             >  SRR4302544.225499/1‑94 (MQ=60)
                                    CGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAC                                                          <  SRR4302544.480986/94‑1 (MQ=60)
                                    CGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAC                                                          <  SRR4302544.3829574/94‑1 (MQ=60)
                                           AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   >  SRR4302544.4413011/1‑94 (MQ=60)
                                           AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   <  SRR4302544.4691006/94‑1 (MQ=60)
                                            AAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGA                                                  <  SRR4302544.2547754/94‑1 (MQ=60)
                                               TGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAT                                               >  SRR4302544.3145533/1‑94 (MQ=60)
                                                GATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATCGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATG                                              >  SRR4302544.2222448/1‑94 (MQ=60)
                                                GATTCGCGCAAACCACCTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATG                                              <  SRR4302544.3839880/94‑1 (MQ=60)
                                                 ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             <  SRR4302544.1820234/94‑1 (MQ=60)
                                                  TTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGA                                            <  SRR4302544.730270/94‑1 (MQ=60)
                                                  TTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGA                                            <  SRR4302544.2986573/94‑1 (MQ=60)
                                                   TCGCGCAAACCACATCGGCGACTAGGGCACTCAATTCGGTACGCTGATTGCATGGCTGGACAAACAGCAGCAGGAAAACGCCGGTGAAATGGAG                                           <  SRR4302544.4261246/94‑1 (MQ=60)
                                                    CGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGC                                          >  SRR4302544.975347/1‑94 (MQ=60)
                                                    CGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGC                                          <  SRR4302544.1881893/94‑1 (MQ=60)
                                                      CGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTG                                        <  SRR4302544.3218222/94‑1 (MQ=60)
                                                      CGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTG                                        >  SRR4302544.3330908/1‑94 (MQ=60)
                                                       GCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGG                                       <  SRR4302544.1031168/94‑1 (MQ=60)
                                                        CAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGC                                      >  SRR4302544.4057191/1‑94 (MQ=60)
                                                        CAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGC                                      >  SRR4302544.319421/1‑94 (MQ=60)
                                                         AAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCT                                     >  SRR4302544.2703665/1‑94 (MQ=60)
                                                          AACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTG                                    >  SRR4302544.1881111/1‑94 (MQ=60)
                                                           ACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGA                                   <  SRR4302544.210333/94‑1 (MQ=60)
                                                            CCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAC                                  >  SRR4302544.1582631/1‑94 (MQ=60)
                                                                GTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTG                              >  SRR4302544.1455741/1‑94 (MQ=60)
                                                                  CGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAA                            <  SRR4302544.3762973/94‑1 (MQ=60)
                                                                   GGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAG                           <  SRR4302544.3611987/94‑1 (MQ=60)
                                                                    GCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGG                          <  SRR4302544.4585631/94‑1 (MQ=60)
                                                                     CGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGT                         >  SRR4302544.4733730/1‑94 (MQ=60)
                                                                      GACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTT                        <  SRR4302544.2134501/94‑1 (MQ=60)
                                                                       ACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTT                       <  SRR4302544.122824/94‑1 (MQ=60)
                                                                           GGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTAC                   <  SRR4302544.2523539/94‑1 (MQ=60)
                                                                             GCACTCAGGTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCG                 <  SRR4302544.4607928/94‑1 (MQ=60)
                                                                              CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                >  SRR4302544.4048571/1‑94 (MQ=60)
                                                                              CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302544.4417856/94‑1 (MQ=60)
                                                                              CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302544.1003716/94‑1 (MQ=60)
                                                                              CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302544.3278739/94‑1 (MQ=60)
                                                                              CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302544.4098344/94‑1 (MQ=60)
                                                                              CACTCAGTGCGGTACGCTGATTGCATGTCTGGAAAAGCAGCAGCAGGAAACCGCCGGTGAAATGAAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302544.3278738/94‑1 (MQ=60)
                                                                               ACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCG               >  SRR4302544.2420266/1‑94 (MQ=60)
                                                                                CTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGA              >  SRR4302544.765170/1‑94 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATT            <  SRR4302544.3974199/94‑1 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            >  SRR4302544.1773984/1‑94 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            >  SRR4302544.1773983/1‑94 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            >  SRR4302544.4396321/1‑94 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            <  SRR4302544.2786289/94‑1 (MQ=60)
                                                                                  CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            <  SRR4302544.3852321/94‑1 (MQ=60)
                                                                                    GTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCG          <  SRR4302544.3669740/94‑1 (MQ=60)
                                                                                     TTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGA         >  SRR4302544.1948996/1‑94 (MQ=60)
                                                                                       CGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTTGCTGACCTTGAAGGTTTCTACCGCGATGCGAAA       >  SRR4302544.2620484/1‑94 (MQ=60)
                                                                                          TACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAG    <  SRR4302544.3558106/94‑1 (MQ=60)
                                                                                           ACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAACAGC   <  SRR4302544.4243822/94‑1 (MQ=60)
                                                                                            CGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  <  SRR4302544.2600644/94‑1 (MQ=60)
                                                                                            |                                                                                             
TATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  >  NC_000913/1958496‑1958681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑