Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,975,051 G→A A38V (GCC→GTC)  motA ← MotA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,975,0510GA99.3% 496.7 / ‑5.3 144A38V (GCC→GTC) motAMotA
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (83/59);  total (84/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 149 total aligned reads displayed.

GAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC  >  NC_000913/1974961‑1975140
                                                                                          |                                                                                         
gAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACa                                                                                         <  2:2351051/93‑1 (MQ=255)
 aGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaa                                                                                        <  2:517614/93‑1 (MQ=255)
    gacgaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGTCaataa                                                                                     <  2:4607762/93‑1 (MQ=255)
    gacgaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaataa                                                                                     >  1:3865880/1‑93 (MQ=255)
        aaaCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAc                                                                                 >  1:697017/1‑93 (MQ=255)
        aaaCAGCAACGGCAGCACCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAc                                                                                 >  2:3183512/1‑93 (MQ=255)
          aCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGCCGATAAACGACCCAATCCCTGCACCGGCAATAATCACCa                                                                               >  1:4040689/1‑93 (MQ=255)
          aCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCa                                                                               <  2:147267/93‑1 (MQ=255)
           cAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAg                                                                              >  2:4370229/1‑93 (MQ=255)
           cAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAg                                                                              <  1:1479998/93‑1 (MQ=255)
           cAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAg                                                                              <  2:718690/93‑1 (MQ=255)
            aGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGt                                                                             <  2:53054/93‑1 (MQ=255)
             gCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGtt                                                                            >  2:1214792/1‑93 (MQ=255)
              cAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTc                                                                           <  1:1374642/93‑1 (MQ=255)
               aaCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCa                                                                          >  2:3700778/1‑93 (MQ=255)
               aaCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCa                                                                          >  2:3444817/1‑93 (MQ=255)
                  ggCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCg                                                                       <  1:1376937/93‑1 (MQ=255)
                  ggCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCg                                                                       <  1:1072934/93‑1 (MQ=255)
                   gCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCgg                                                                      >  1:939835/1‑93 (MQ=255)
                    cAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCggg                                                                     <  1:674969/93‑1 (MQ=255)
                     aGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGt                                                                    >  2:2351691/1‑93 (MQ=255)
                     aGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGt                                                                    >  2:3948631/1‑93 (MQ=255)
                        gcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGtt                                                                   <  2:4342344/91‑1 (MQ=255)
                        gcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGtt                                                                   >  1:4342344/1‑91 (MQ=255)
                        gcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGa                                                                 <  1:2928659/93‑1 (MQ=255)
                        gcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGa                                                                 >  1:2977409/1‑93 (MQ=255)
                         ccTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAt                                                                <  2:3659517/93‑1 (MQ=255)
                         ccTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAt                                                                >  1:2145004/1‑93 (MQ=255)
                           ttCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATag                                                              >  2:242410/1‑93 (MQ=255)
                            tCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATaga                                                             >  2:3911950/1‑93 (MQ=255)
                              aGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGt                                                           <  2:3320819/93‑1 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTg                                                          >  2:3967504/1‑93 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTg                                                          >  1:2078221/1‑93 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTg                                                          <  1:1586001/93‑1 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTg                                                          >  1:3361729/1‑93 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCACAGAGTTGATAGAGTg                                                          >  1:3361728/1‑93 (MQ=255)
                                 gTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCt                                                        >  1:2530156/1‑93 (MQ=255)
                                  ggCCTTTGATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTc                                                       >  2:2883050/2‑93 (MQ=255)
                                   gCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTcc                                                      >  2:3235814/1‑93 (MQ=255)
                                    ccTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCATGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCa                                                     >  1:3701190/1‑93 (MQ=255)
                                     cTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCaa                                                    <  1:1636066/93‑1 (MQ=255)
                                     cTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCaa                                                    >  1:4749646/1‑93 (MQ=255)
                                         aaTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                <  1:2163614/93‑1 (MQ=255)
                                         aaTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                >  2:4598828/1‑93 (MQ=255)
                                         aaTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                >  1:4257331/1‑93 (MQ=255)
                                         aaTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                >  2:3238639/1‑93 (MQ=255)
                                          aTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCtt                                               <  2:902144/93‑1 (MQ=255)
                                           tCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTc                                              <  1:4118365/93‑1 (MQ=255)
                                             gCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCa                                            <  2:4464986/93‑1 (MQ=255)
                                             gCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCa                                            >  1:3153193/1‑93 (MQ=255)
                                               tttGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAcc                                          >  2:1013237/1‑93 (MQ=255)
                                               tttGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAcc                                          >  2:4160120/1‑93 (MQ=255)
                                               tttGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAcc                                          >  1:587092/1‑93 (MQ=255)
                                                ttGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCg                                         <  2:196649/93‑1 (MQ=255)
                                                  tccATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGt                                       <  1:3588736/92‑1 (MQ=255)
                                                   ccATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtc                                      >  2:1938574/1‑93 (MQ=255)
                                                     attattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcat                                    >  1:3779646/1‑93 (MQ=255)
                                                      ttattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   >  2:2373128/1‑93 (MQ=255)
                                                      ttattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   >  2:3593831/1‑93 (MQ=255)
                                                       tattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatca                                  >  2:210501/1‑93 (MQ=255)
                                                       tattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGCGCTCCAAGGCTTCCACCGGtcatca                                  >  2:2600124/1‑93 (MQ=255)
                                                         ttGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCaaa                                <  1:241708/93‑1 (MQ=255)
                                                             cgacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAc                            <  2:2892114/93‑1 (MQ=255)
                                                             cgacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAc                            >  2:3154234/1‑93 (MQ=255)
                                                              gacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAcc                           <  2:1823815/93‑1 (MQ=255)
                                                              gacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAcc                           <  2:1656671/93‑1 (MQ=255)
                                                              gacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAcc                           <  1:4652836/93‑1 (MQ=255)
                                                              gacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAcc                           <  1:1469949/93‑1 (MQ=255)
                                                                cgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgc                         <  1:3830347/93‑1 (MQ=255)
                                                                 gaTAAAGGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgcc                        >  2:4668968/1‑93 (MQ=255)
                                                                 gaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgcc                        >  2:4100763/1‑93 (MQ=255)
                                                                 gaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgcc                        <  1:236235/93‑1 (MQ=255)
                                                                  aTAACCGACCCACTCCGTGCACCGACAATAATCACCAGTGCAGCGGGTTGATAGAGTGCTCCAAGGCTGCCACCGGTCATCAAATAAccgccg                       <  1:4549203/93‑1 (MQ=255)
                                                                  aTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgccg                       >  1:2503350/1‑93 (MQ=255)
                                                                  aTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgccg                       >  1:3599310/1‑93 (MQ=255)
                                                                  aTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccgccg                       >  2:2107211/1‑93 (MQ=255)
                                                                      aCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGagaa                   >  1:4172127/1‑93 (MQ=255)
                                                                      aCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGaaaa                   >  1:2554606/1‑93 (MQ=255)
                                                                       cGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAAc                  <  1:2600136/93‑1 (MQ=255)
                                                                           ccAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTa              >  2:1832813/1‑93 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             >  1:3348799/1‑93 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             >  2:163875/1‑93 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             >  1:1834393/1‑93 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             <  1:581200/93‑1 (MQ=255)
                                                                            cAATCCCTCCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             <  2:4285393/93‑1 (MQ=255)
                                                                             aaTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAcc            <  1:1005496/93‑1 (MQ=255)
                                                                             aaTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAcc            >  2:615989/1‑93 (MQ=255)
                                                                              aTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCg           >  1:1157342/1‑93 (MQ=255)
                                                                               tCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCga          <  1:4290321/93‑1 (MQ=255)
                                                                                cccTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACTgag         >  2:4184742/1‑93 (MQ=255)
                                                                                 cccacACCGACAATAACCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCTCCGGTAATCAAATACCCGCCGAAAACTGGACCgaga        <  2:1414344/89‑1 (MQ=255)
                                                                                  cTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaa       >  1:2050741/1‑93 (MQ=255)
                                                                                  cTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaa       <  1:4489766/93‑1 (MQ=255)
                                                                                   tGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaac      >  2:920849/1‑93 (MQ=255)
                                                                                      aCCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaacaac   >  1:932770/1‑93 (MQ=255)
                                                                                      aCCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaacaac   >  1:391574/1‑93 (MQ=255)
                                                                                      aCCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGCCATCCAATAACCACCGAAAACTGTACAGAGaacaac   >  2:1540469/1‑93 (MQ=255)
                                                                                      aCCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTACACCGGTCCTCAAATAACCGCCGAAAACTGTACCGCGaacaac   >  1:391689/1‑93 (MQ=255)
                                                                                       ccGCCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAcc  <  2:2572190/93‑1 (MQ=255)
                                                                                       ccGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAcc  <  1:2077997/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC  >  NC_000913/1974961‑1975140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG  >  NC_000913/1974959‑1975143
                                                                                            |                                                                                            
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGAC                                                                                             <  SRR4302544.573312/94‑1 (MQ=60)
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGAC                                                                                             >  SRR4302544.40631/1‑94 (MQ=60)
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGAC                                                                                             <  SRR4302544.573311/94‑1 (MQ=60)
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGAC                                                                                             >  SRR4302544.527924/1‑94 (MQ=60)
     CGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAA                                                                                        >  SRR4302544.3904265/1‑94 (MQ=60)
      GACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAAT                                                                                       >  SRR4302544.3135984/1‑94 (MQ=60)
        CGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCA                                                                                     <  SRR4302544.2574336/94‑1 (MQ=60)
         GAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAC                                                                                    >  SRR4302544.703961/1‑94 (MQ=60)
           AACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGCCGATAAACGACCCAATCCCTGCACCGGCAATAATCACCA                                                                                  >  SRR4302544.4080930/1‑94 (MQ=60)
             CAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGT                                                                                <  SRR4302544.1494623/94‑1 (MQ=60)
               ACAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCATTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTC                                                                              <  SRR4302544.370981/94‑1 (MQ=60)
                CAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCA                                                                             >  SRR4302544.3857919/1‑94 (MQ=60)
                CAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCA                                                                             <  SRR4302544.1388341/94‑1 (MQ=60)
                    GGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGG                                                                         <  SRR4302544.1390671/94‑1 (MQ=60)
                    GGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGG                                                                         <  SRR4302544.1083599/94‑1 (MQ=60)
                    GGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGG                                                                         >  SRR4302544.949089/1‑94 (MQ=60)
                      CAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGT                                                                       <  SRR4302544.681656/94‑1 (MQ=60)
                      CACCGCCTTCAGCGGTCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAACCCCTCCACCGACAATAATCACCAGTTCAGCGGGT                                                                       <  SRR4302544.681655/94‑1 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                    >  SRR4302544.2073519/1‑94 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                    >  SRR4302544.4386515/1‑94 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                    <  SRR4302544.4059365/94‑1 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                    >  SRR4302544.3005967/1‑94 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                   >  SRR4302544.2165453/1‑94 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                   <  SRR4302544.2956800/94‑1 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                 <  SRR4302544.4691190/94‑1 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTG                                                             >  SRR4302544.3393814/1‑94 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTG                                                             >  SRR4302544.2098198/1‑94 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTG                                                             >  SRR4302544.1205600/1‑94 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCACAGAGTTGATAGAGTG                                                             >  SRR4302544.3393813/1‑94 (MQ=60)
                                 GCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGC                                                            <  SRR4302544.1601656/94‑1 (MQ=60)
                                  CGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCT                                                           >  SRR4302544.2554462/1‑94 (MQ=60)
                                   GTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTC                                                          >  SRR4302544.2128733/1‑94 (MQ=60)
                                   GTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTC                                                          >  SRR4302544.854231/1‑94 (MQ=60)
                                     GCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCATGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCA                                                        >  SRR4302544.3738035/1‑94 (MQ=60)
                                      CCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAA                                                       >  SRR4302544.4796425/1‑94 (MQ=60)
                                       nnntaatncnTTTGCCANTANNNNCNACGATAAACGACCCAATCCCTNCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAG                                                      <  SRR4302544.1045299/84‑1 (MQ=60)
                                       CTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAG                                                      <  SRR4302544.1652168/94‑1 (MQ=60)
                                          TAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCT                                                   >  SRR4302544.4300736/1‑94 (MQ=60)
                                           AATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTT                                                  <  SRR4302544.3917333/94‑1 (MQ=60)
                                           AATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTT                                                  <  SRR4302544.2184152/94‑1 (MQ=60)
                                             TCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCC                                                <  SRR4302544.4159256/94‑1 (MQ=60)
                                              CGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCA                                               >  SRR4302544.3183187/1‑94 (MQ=60)
                                                CTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACC                                             >  SRR4302544.592852/1‑94 (MQ=60)
                                                    TCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTC                                         <  SRR4302544.3623194/94‑1 (MQ=60)
                                                      CATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCAT                                       >  SRR4302544.3817084/1‑94 (MQ=60)
                                                           TTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAAT                                  <  SRR4302544.244223/94‑1 (MQ=60)
                                                              CCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAC                               <  SRR4302544.4211712/94‑1 (MQ=60)
                                                                GACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                             <  SRR4302544.4698882/94‑1 (MQ=60)
                                                                GACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                             <  SRR4302544.1484515/94‑1 (MQ=60)
                                                                GACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                             <  SRR4302544.559232/94‑1 (MQ=60)
                                                                  CGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCC                           <  SRR4302544.3868329/94‑1 (MQ=60)
                                                                   GATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCG                          >  SRR4302544.3634043/1‑94 (MQ=60)
                                                                   GATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCG                          >  SRR4302544.2527350/1‑94 (MQ=60)
                                                                   GATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCG                          <  SRR4302544.238693/94‑1 (MQ=60)
                                                                    ATAACCGACCCACTCCGTGCACCGACAATAATCACCAGTGCAGCGGGTTGATAGAGTGCTCCAAGGCTGCCACCGGTCATCAAATAACCGCCGA                         <  SRR4302544.4594631/94‑1 (MQ=60)
                                                                       AACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAGAA                      >  SRR4302544.4213453/1‑94 (MQ=60)
                                                                       AACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAA                      >  SRR4302544.2579133/1‑94 (MQ=60)
                                                                         CGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACT                    <  SRR4302544.2625126/94‑1 (MQ=60)
                                                                         CGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACT                    >  SRR4302544.3388210/1‑94 (MQ=60)
                                                                             CCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAC                >  SRR4302544.3380753/1‑94 (MQ=60)
                                                                             CCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAC                >  SRR4302544.1852210/1‑94 (MQ=60)
                                                                              CAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC               <  SRR4302544.586889/94‑1 (MQ=60)
                                                                              CAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC               <  SRR4302544.244726/94‑1 (MQ=60)
                                                                              CAACCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC               <  SRR4302544.1700681/94‑1 (MQ=60)
                                                                               AATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCG              >  SRR4302544.2809728/1‑94 (MQ=60)
                                                                               AATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCG              >  SRR4302544.1168866/1‑94 (MQ=60)
                                                                               AATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCG              <  SRR4302544.1015487/94‑1 (MQ=60)
                                                                                 TCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAG            <  SRR4302544.4334135/94‑1 (MQ=60)
                                                                                   CCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAA          >  SRR4302544.2070490/1‑94 (MQ=60)
                                                                                    CTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAAC         <  SRR4302544.4534845/94‑1 (MQ=60)
                                                                                    CTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAAC         >  SRR4302544.2262980/1‑94 (MQ=60)
                                                                                     TGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACA        >  SRR4302544.1394354/1‑94 (MQ=60)
                                                                                     TGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAACGAGCACA        >  SRR4302544.4321257/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAC      >  SRR4302544.1551/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAC      >  SRR4302544.395546/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAC      >  SRR4302544.941960/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTACACCGGTCCTCAAATAACCGCCGAAAACTGTACCGCGAACAAC      >  SRR4302544.395661/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTACAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAC      <  SRR4302544.3534630/94‑1 (MQ=60)
                                                                                        ACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC     <  SRR4302544.4431503/94‑1 (MQ=60)
                                                                                         CCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCA    <  SRR4302544.2097970/94‑1 (MQ=60)
                                                                                          CGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAG   >  SRR4302544.655967/1‑94 (MQ=60)
                                                                                          CGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCAAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAG   >  SRR4302544.995170/1‑94 (MQ=60)
                                                                                           GACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG  >  SRR4302544.3563178/1‑94 (MQ=60)
                                                                                           GACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG  <  SRR4302544.3039403/94‑1 (MQ=60)
                                                                                           GACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG  >  SRR4302544.2640718/1‑94 (MQ=60)
                                                                                            |                                                                                            
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG  >  NC_000913/1974959‑1975143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑