Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,999,772 T→C H4R (CAC→CGC)  b1922 ← sigma factor 28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,7720TC98.6% 453.8 / ‑3.8 145H4R (CAC→CGC) b1922sigma factor 28
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (67/76);  total (68/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 152 total aligned reads displayed.

GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999683‑1999861
                                                                                         |                                                                                         
gATCGTCAAGTTCCACGCTCGCGGGCAGTCGAGCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGtt                                                                                        <  1:4028221/93‑1 (MQ=255)
gATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGtt                                                                                        <  1:3310195/93‑1 (MQ=255)
 aTCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGttt                                                                                       <  2:4321346/93‑1 (MQ=255)
  tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  2:2326617/93‑1 (MQ=255)
       aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATCACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 >  2:346252/1‑93 (MQ=255)
       aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 >  2:346253/1‑93 (MQ=255)
       aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 <  2:4172021/93‑1 (MQ=255)
        aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                >  2:263939/1‑93 (MQ=255)
         gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               <  1:2421650/93‑1 (MQ=255)
                gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCTGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTc                                                                        <  2:287608/93‑1 (MQ=255)
                gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTc                                                                        <  1:4425036/93‑1 (MQ=255)
                gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTc                                                                        >  1:4720621/1‑93 (MQ=255)
                gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTc                                                                        <  2:2491786/93‑1 (MQ=255)
                 cgcgcgGGCAGTCGAACGTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  1:1411876/91‑1 (MQ=255)
                 cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       >  2:714400/1‑93 (MQ=255)
                    gcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCg                                                                    <  1:833116/93‑1 (MQ=255)
                      gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  1:638354/1‑93 (MQ=255)
                      gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  <  2:3818248/93‑1 (MQ=255)
                      gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  2:2598112/1‑93 (MQ=255)
                      gggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGt                                                                  >  1:1734736/1‑93 (MQ=255)
                         cAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtata                                                               <  2:1053298/93‑1 (MQ=255)
                          aGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATag                                                              <  2:3773062/93‑1 (MQ=255)
                           gTCGAACCTGCAGGCGCAATGCTTCGTGGCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             <  2:3182279/93‑1 (MQ=255)
                           gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             >  2:2413491/1‑93 (MQ=255)
                           gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             <  1:2544323/93‑1 (MQ=255)
                             cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  1:640015/93‑1 (MQ=255)
                             cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           >  2:2026265/1‑93 (MQ=255)
                                aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTgca                                                        >  1:1367728/1‑91 (MQ=255)
                                aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  1:3872489/1‑93 (MQ=255)
                                aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  2:2971003/1‑93 (MQ=255)
                                aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  1:4647833/1‑93 (MQ=255)
                                 ccTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAt                                                       <  1:3352509/93‑1 (MQ=255)
                                 ccTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAt                                                       <  2:650795/93‑1 (MQ=255)
                                  cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      >  1:1729271/1‑93 (MQ=255)
                                   tGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTc                                                     >  1:1621696/1‑93 (MQ=255)
                                     cAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATGCATTACACCTTCAGCGGTATAGAGTGAATTCag                                                   >  1:2663551/1‑92 (MQ=255)
                                      aGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACg                                                  >  1:1557929/1‑93 (MQ=255)
                                      aGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACg                                                  >  1:4532055/1‑93 (MQ=255)
                                       ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  1:533401/93‑1 (MQ=255)
                                        gcgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt                                                <  1:3719642/93‑1 (MQ=255)
                                          gcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa                                              >  2:75645/1‑93 (MQ=255)
                                            aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:3867925/1‑93 (MQ=255)
                                             aTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           <  1:2475607/93‑1 (MQ=255)
                                             aTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           <  2:2408794/93‑1 (MQ=255)
                                              tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          <  2:532045/93‑1 (MQ=255)
                                              tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          >  2:673407/1‑93 (MQ=255)
                                              tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          >  1:3775190/1‑93 (MQ=255)
                                               gCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         >  1:4746230/1‑93 (MQ=255)
                                                cTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGcc                                        <  1:3519816/93‑1 (MQ=255)
                                                 ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       >  2:2278217/1‑93 (MQ=255)
                                                 ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       >  1:404904/1‑93 (MQ=255)
                                                 ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  1:635455/93‑1 (MQ=255)
                                                 cgcttGACCCCCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  1:635454/89‑1 (MQ=255)
                                                  tCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCt                                      >  1:2848028/1‑93 (MQ=255)
                                                    gTGACGCACCAGCTGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    <  1:306996/93‑1 (MQ=255)
                                                    gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    <  2:3350037/93‑1 (MQ=255)
                                                    gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAAAGCCCTGc                                    >  1:2329208/1‑93 (MQ=255)
                                                     tGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCg                                   <  2:2023032/93‑1 (MQ=255)
                                                      gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  2:1265076/1‑93 (MQ=255)
                                                        cGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTta                                <  2:1676227/93‑1 (MQ=255)
                                                           aCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTatat                             <  2:4543247/93‑1 (MQ=255)
                                                            ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATg                            >  1:1327335/1‑93 (MQ=255)
                                                             cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           <  1:2455505/93‑1 (MQ=255)
                                                             aaGCGGGCAAAAACGCTGTCACAGGGAGCTTTTATCCATTACCCCTTCAGCGGTATAGAGTGAATTCACGATACACAGCCCTGCGTTATATGa                           <  1:3535029/92‑1 (MQ=255)
                                                               gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         >  1:3330299/1‑93 (MQ=255)
                                                               gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         <  2:1884062/93‑1 (MQ=255)
                                                               gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         >  1:2777131/1‑93 (MQ=255)
                                                               gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         >  1:3710923/1‑93 (MQ=255)
                                                                 gggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTa                       >  2:1663792/1‑93 (MQ=255)
                                                                  gtaCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:301872/91‑1 (MQ=255)
                                                                  ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      >  2:3271319/1‑93 (MQ=255)
                                                                  ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:1116084/93‑1 (MQ=255)
                                                                  ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  2:796973/93‑1 (MQ=255)
                                                                   gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:136199/93‑1 (MQ=255)
                                                                       tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 >  2:4175625/1‑93 (MQ=255)
                                                                       tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 >  1:89833/1‑93 (MQ=255)
                                                                       tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGGATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 >  2:303663/1‑93 (MQ=255)
                                                                        aaCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAt                <  2:4522779/93‑1 (MQ=255)
                                                                         aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               <  1:528018/93‑1 (MQ=255)
                                                                           gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             <  1:3432666/93‑1 (MQ=255)
                                                                           gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             <  1:3432801/93‑1 (MQ=255)
                                                                             tGCGACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTa           <  2:2973054/93‑1 (MQ=255)
                                                                             tGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTa           >  2:2487385/1‑93 (MQ=255)
                                                                              gCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAt          >  1:95092/1‑93 (MQ=255)
                                                                              gCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAt          >  1:3631699/1‑93 (MQ=255)
                                                                               ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  2:4388480/93‑1 (MQ=255)
                                                                               ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  1:1360682/93‑1 (MQ=255)
                                                                                cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        >  1:3680060/1‑93 (MQ=255)
                                                                                 acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcct       >  2:1853823/1‑92 (MQ=255)
                                                                                  cggCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGt      <  1:4570023/91‑1 (MQ=255)
                                                                                   agcgagcgTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGtt     <  2:754024/93‑1 (MQ=255)
                                                                                    gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    >  2:39454/1‑93 (MQ=255)
                                                                                     cGAGCGTTTATCCATTACTCCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:1083783/93‑1 (MQ=255)
                                                                                     cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:2843813/93‑1 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATTCGTTTCt  >  2:4653403/1‑93 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  2:2759029/1‑93 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  2:2759030/1‑93 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  1:2552137/1‑93 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  2:4713064/1‑93 (MQ=255)
                                                                                      gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  <  1:843063/93‑1 (MQ=255)
                                                                                         |                                                                                         
GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999683‑1999861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC  >  NC_000913/1999680‑1999863
                                                                                            |                                                                                           
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCG                                                                                            <  SRR4302544.1330388/94‑1 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAGCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                         <  SRR4302544.4068345/94‑1 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                         <  SRR4302544.3341721/94‑1 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                      >  SRR4302544.2805528/1‑94 (MQ=60)
         CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                   <  SRR4302544.1284599/94‑1 (MQ=60)
            GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                <  SRR4302544.2444708/94‑1 (MQ=60)
                  CGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTC                                                                          >  SRR4302544.4767234/1‑94 (MQ=60)
                   GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                         <  SRR4302544.4469676/94‑1 (MQ=60)
                    CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                        <  SRR4302544.3383688/94‑1 (MQ=60)
                    CGCGCGGGCAGTCGAACGTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                        <  SRR4302544.1425936/94‑1 (MQ=60)
                       GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                     <  SRR4302544.4608162/94‑1 (MQ=60)
                       GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                     <  SRR4302544.841316/94‑1 (MQ=60)
                        CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                    >  SRR4302544.1751736/1‑94 (MQ=60)
                        CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                    >  SRR4302544.644646/1‑94 (MQ=60)
                        CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                    >  SRR4302544.644647/1‑94 (MQ=60)
                         GGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTA                                                                   <  SRR4302544.3431829/94‑1 (MQ=60)
                           GCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATA                                                                 <  SRR4302544.589214/94‑1 (MQ=60)
                            CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                >  SRR4302544.3161584/1‑94 (MQ=60)
                            CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATACATTACACCTTCAGCGGTATAG                                                                <  SRR4302544.844344/94‑1 (MQ=60)
                              GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                              <  SRR4302544.2568750/94‑1 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                            <  SRR4302544.646328/94‑1 (MQ=60)
                                  AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGCA                                                          >  SRR4302544.1381328/1‑94 (MQ=60)
                                  AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                          >  SRR4302544.4693846/1‑94 (MQ=60)
                                  AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                          >  SRR4302544.3910947/1‑94 (MQ=60)
                                    CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                        >  SRR4302544.1746229/1‑94 (MQ=60)
                                    CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                        <  SRR4302544.3384503/94‑1 (MQ=60)
                                     CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                       >  SRR4302544.1637675/1‑94 (MQ=60)
                                      TGCAGGCGCAATGCTTCGTGACGCACCAGCGGGGCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCA                                                      <  SRR4302544.3783366/94‑1 (MQ=60)
                                       GCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATGCATTACACCTTCAGCGGTATAGAGTGAATTCAG                                                     >  SRR4302544.2689086/1‑94 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                    >  SRR4302544.4577385/1‑94 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                    >  SRR4302544.1573419/1‑94 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                    <  SRR4302544.93550/94‑1 (MQ=60)
                                          GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                  <  SRR4302544.538684/94‑1 (MQ=60)
                                           GCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATA                                                 <  SRR4302544.3756635/94‑1 (MQ=60)
                                             gcaattgtgccggaccCACCAGCGGGACATAACGCTGCCAAAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                               <  SRR4302544.3779041/78‑1 (MQ=60)
                                             GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                               >  SRR4302544.259768/1‑94 (MQ=60)
                                                ATTCTTAGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACTCCTTCAGGGGTATAGAGTGAATTCACGATAAACAG                                            <  SRR4302544.2499284/94‑1 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                            >  SRR4302544.3812605/1‑94 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                            <  SRR4302544.2499283/94‑1 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                           >  SRR4302544.4792993/1‑94 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                           >  SRR4302544.4792992/1‑94 (MQ=60)
                                                  GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                          >  SRR4302544.2982065/1‑94 (MQ=60)
                                                   CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                         >  SRR4302544.409015/1‑94 (MQ=60)
                                                   CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                         <  SRR4302544.3553429/94‑1 (MQ=60)
                                                    cgcttgaccccCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                        <  SRR4302544.641719/83‑1 (MQ=60)
                                                    TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                        >  SRR4302544.2875538/1‑94 (MQ=60)
                                                    TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                        <  SRR4302544.641720/94‑1 (MQ=60)
                                                      CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAAAGCCCTGC                                      >  SRR4302544.2351292/1‑94 (MQ=60)
                                                       GTGACGCACCAGCTGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                     <  SRR4302544.310177/94‑1 (MQ=60)
                                                       GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                     >  SRR4302544.4731923/1‑94 (MQ=60)
                                                           CGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTAT                                 >  SRR4302544.2465287/1‑94 (MQ=60)
                                                              ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                              >  SRR4302544.1340393/1‑94 (MQ=60)
                                                               CCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGA                             <  SRR4302544.704657/94‑1 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                            <  SRR4302544.3568843/94‑1 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                            <  SRR4302544.2478931/94‑1 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                           >  SRR4302544.3747836/1‑94 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                           >  SRR4302544.3362014/1‑94 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                           >  SRR4302544.2803845/1‑94 (MQ=60)
                                                                     GTACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                       <  SRR4302544.304999/94‑1 (MQ=60)
                                                                     GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                       <  SRR4302544.1127226/94‑1 (MQ=60)
                                                                     GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                       >  SRR4302544.1659958/1‑94 (MQ=60)
                                                                      GACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCG                      <  SRR4302544.3745016/94‑1 (MQ=60)
                                                                         ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                   >  SRR4302544.90677/1‑94 (MQ=60)
                                                                           AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                 >  SRR4302544.4204150/1‑94 (MQ=60)
                                                                            ACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGA                <  SRR4302544.533258/94‑1 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT              <  SRR4302544.3465618/94‑1 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT              <  SRR4302544.3465481/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT            >  SRR4302544.3667887/1‑94 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT            <  SRR4302544.3521559/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT            >  SRR4302544.95967/1‑94 (MQ=60)
                                                                                 GCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGAGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGACATGAATATC           >  SRR4302544.1500108/1‑94 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC          >  SRR4302544.3716765/1‑94 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC          <  SRR4302544.1785803/94‑1 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC          <  SRR4302544.1374192/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG         >  SRR4302544.1378029/1‑94 (MQ=60)
                                                                                     CGGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTT       <  SRR4302544.4615577/94‑1 (MQ=60)
                                                                                       GCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTC     <  SRR4302544.4045774/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACTCCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT    <  SRR4302544.1094564/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT    <  SRR4302544.2871263/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT    >  SRR4302544.2576634/1‑94 (MQ=60)
                                                                                         GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG   <  SRR4302544.851390/94‑1 (MQ=60)
                                                                                          AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC  >  SRR4302544.231899/1‑94 (MQ=60)
                                                                                            |                                                                                           
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC  >  NC_000913/1999680‑1999863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑