Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,999,772 T→C H4R (CAC→CGC)  b1922 ← sigma factor 28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,7720TC100.0% 849.1 / NA 264H4R (CAC→CGC) b1922sigma factor 28
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (112/152);  total (112/152)

Only 100 of 281 total aligned reads displayed.

AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861
                                                                                          |                                                                                         
 gATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGCGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGtt                                                                                        <  1:6274758/93‑1 (MQ=255)
  aTCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGttt                                                                                       <  2:2352010/93‑1 (MQ=255)
   tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  1:1042025/93‑1 (MQ=255)
   tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  2:2478361/93‑1 (MQ=255)
     gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  2:1426510/1‑93 (MQ=255)
      tCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATcc                                                                                   >  2:5240758/1‑93 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 <  1:3732879/93‑1 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                <  2:3548318/93‑1 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                <  2:2266280/93‑1 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                >  2:1902312/1‑93 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                >  1:4774538/1‑93 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                <  1:5019532/93‑1 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               >  1:5771475/1‑93 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               <  2:5912907/93‑1 (MQ=255)
           ttCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTac                                                                              <  2:3048339/93‑1 (MQ=255)
           taccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTac                                                                              <  1:4399326/91‑1 (MQ=255)
             ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  2:3840742/93‑1 (MQ=255)
              cACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAcc                                                                           >  1:1580981/1‑93 (MQ=255)
               aCGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCt                                                                          <  2:5796966/93‑1 (MQ=255)
                cGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCtt                                                                         >  1:28643/1‑93 (MQ=255)
                 gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTc                                                                        <  1:5329193/93‑1 (MQ=255)
                   tCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAg                                                                      >  1:5070207/1‑93 (MQ=255)
                   tCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAg                                                                      <  1:5985755/93‑1 (MQ=255)
                        ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCggca                                                                 >  2:3279618/1‑91 (MQ=255)
                        ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 <  2:1959713/93‑1 (MQ=255)
                          cAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtata                                                               <  2:5781958/93‑1 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             >  2:557006/1‑93 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             <  2:4395391/93‑1 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             >  1:3179116/1‑93 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             <  2:3741789/93‑1 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCTGTATagag                                                            <  1:6610298/93‑1 (MQ=255)
                              cGACCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  1:4545333/93‑1 (MQ=255)
                              cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  2:2952130/93‑1 (MQ=255)
                              cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  1:35109/93‑1 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTg                                                          >  1:2276268/1‑93 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTg                                                          <  1:1771915/93‑1 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTg                                                          >  2:5042834/1‑93 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATACATTACACCTTCAGCGGTATAGAGTg                                                          >  2:55459/1‑93 (MQ=255)
                                aaCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGa                                                         <  2:6547632/93‑1 (MQ=255)
                                     gCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCa                                                    <  1:3157974/93‑1 (MQ=255)
                                       aGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACg                                                  <  2:5962562/93‑1 (MQ=255)
                                        ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  1:2030274/93‑1 (MQ=255)
                                        ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  1:3764619/93‑1 (MQ=255)
                                        ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  2:3494987/93‑1 (MQ=255)
                                         gcgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt                                                <  1:5603106/93‑1 (MQ=255)
                                          cgcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATa                                               <  1:2309909/93‑1 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            <  2:1837174/93‑1 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:264665/1‑93 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:3771282/1‑93 (MQ=255)
                                              aTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           >  2:6501253/1‑93 (MQ=255)
                                              aTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           <  2:4904562/93‑1 (MQ=255)
                                               tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          >  1:2331846/1‑93 (MQ=255)
                                               tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          >  1:2534530/1‑93 (MQ=255)
                                                tcTTGGTCACGGACCAGCGGGAAATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         <  1:35932/92‑1 (MQ=255)
                                                 cTTCGTGACGCACCAGCGGGACATAGCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGcc                                        <  2:6256217/93‑1 (MQ=255)
                                                  ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  2:1168274/93‑1 (MQ=255)
                                                  ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       >  2:3957072/1‑93 (MQ=255)
                                                  ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       >  2:2527900/1‑93 (MQ=255)
                                                  ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGccc                                       <  2:2743771/93‑1 (MQ=255)
                                                   tCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCt                                      <  1:81616/93‑1 (MQ=255)
                                                    cGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTg                                     >  2:707378/1‑93 (MQ=255)
                                                      tGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCg                                   >  1:6420388/1‑93 (MQ=255)
                                                       gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  <  1:674911/93‑1 (MQ=255)
                                                       gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  1:4030957/1‑93 (MQ=255)
                                                       gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  1:1280145/1‑93 (MQ=255)
                                                        aCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGtt                                 <  2:2825167/93‑1 (MQ=255)
                                                          gCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtac                               <  2:6319172/93‑2 (MQ=255)
                                                            aCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTatat                             >  2:4281233/1‑93 (MQ=255)
                                                             ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATg                            >  2:5174617/1‑93 (MQ=255)
                                                             ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATg                            >  1:6199928/1‑93 (MQ=255)
                                                               aGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          >  1:3416849/1‑93 (MQ=255)
                                                                 cGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGtt                        <  2:4111120/93‑1 (MQ=255)
                                                                 cGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGtt                        <  2:4842138/93‑1 (MQ=255)
                                                                  gggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTa                       >  2:4550008/1‑93 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:2914476/93‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  1:363000/93‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     >  2:6697144/1‑93 (MQ=255)
                                                                     aCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCg                    >  2:2562903/1‑93 (MQ=255)
                                                                       aTAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGc                  <  1:2217129/93‑1 (MQ=255)
                                                                        tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 <  2:1202604/93‑1 (MQ=255)
                                                                        tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 <  1:4266906/93‑1 (MQ=255)
                                                                         aaCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAt                >  2:1482345/1‑93 (MQ=255)
                                                                          aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               >  1:1103187/1‑93 (MQ=255)
                                                                            gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             <  2:2403342/93‑1 (MQ=255)
                                                                             cTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAtt            >  1:610439/1‑93 (MQ=255)
                                                                              tGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTa           <  1:95384/93‑1 (MQ=255)
                                                                               gCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAt          >  1:3858442/1‑93 (MQ=255)
                                                                                ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  2:309140/93‑1 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        <  1:136442/93‑1 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        <  1:5244406/93‑1 (MQ=255)
                                                                                  acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  2:790066/1‑93 (MQ=255)
                                                                                  acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  1:1699201/1‑93 (MQ=255)
                                                                                    agcgagcgTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGtt     >  2:1753738/1‑93 (MQ=255)
                                                                                    agcgagcgTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGtt     <  1:1861457/93‑1 (MQ=255)
                                                                                     gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    >  2:4307650/1‑93 (MQ=255)
                                                                                     gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    >  1:3707729/1‑93 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:4939609/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:5486751/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  2:1631387/93‑1 (MQ=255)
                                                                                       gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  <  2:4748990/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999680‑1999865
                                                                                            |                                                                                             
GCAGATCGTCAAGTTCCACGCTCGAGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACCGCGAGCG                                                                                              >  SRR4302536.3973939/1‑94 (MQ=60)
 CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGT                                                                                             >  SRR4302536.705387/1‑94 (MQ=60)
  AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            >  SRR4302536.5622191/1‑94 (MQ=60)
  AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            <  SRR4302536.1935933/94‑1 (MQ=60)
  AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            >  SRR4302536.4932922/1‑94 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                           <  SRR4302536.4378573/94‑1 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                           <  SRR4302536.3720604/94‑1 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                           <  SRR4302536.5737576/94‑1 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGCGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                           <  SRR4302536.6461707/94‑1 (MQ=60)
    ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTA                                                                                          <  SRR4302536.1244193/94‑1 (MQ=60)
     TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         <  SRR4302536.1073001/94‑1 (MQ=60)
     TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         <  SRR4302536.5358514/94‑1 (MQ=60)
     TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         >  SRR4302536.5930790/1‑94 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGAGATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302536.3757000/1‑94 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302536.6437094/1‑94 (MQ=60)
        TCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCA                                                                                      <  SRR4302536.406253/94‑1 (MQ=60)
        TCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCA                                                                                      >  SRR4302536.4605457/1‑94 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    <  SRR4302536.3844444/94‑1 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302536.1796778/1‑94 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302536.4918308/1‑94 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302536.6186018/1‑94 (MQ=60)
           AGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTA                                                                                   >  SRR4302536.5943208/1‑94 (MQ=60)
           AGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTA                                                                                   <  SRR4302536.5170004/94‑1 (MQ=60)
            GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                  <  SRR4302536.2303219/94‑1 (MQ=60)
             TACCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACA                                                                                 <  SRR4302536.4530811/94‑1 (MQ=60)
               CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               >  SRR4302536.1627285/1‑94 (MQ=60)
                CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              <  SRR4302536.5126093/94‑1 (MQ=60)
                 ccgcgCGCGGGCAGTCGAACCTGCAGGCGCAATGCTGCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACGCCTT                                                                             <  SRR4302536.515207/89‑1 (MQ=60)
                 ACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGGCATCACGCTGACACAGCGAACGTGTATCCATTCCACCTT                                                                             >  SRR4302536.6077040/1‑94 (MQ=60)
                 ACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTT                                                                             >  SRR4302536.29438/1‑94 (MQ=60)
                  CGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTC                                                                            >  SRR4302536.2334231/1‑94 (MQ=60)
                   GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                           <  SRR4302536.5488421/94‑1 (MQ=60)
                    CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                          <  SRR4302536.1535914/94‑1 (MQ=60)
                    CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                          >  SRR4302536.5222013/1‑94 (MQ=60)
                     TCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGC                                                                         <  SRR4302536.6164224/94‑1 (MQ=60)
                      CGCGGGCAGTCGAGCCTGCAGGCGCAATGCTTCGGGACGGACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        <  SRR4302536.2571261/94‑1 (MQ=60)
                       GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                       >  SRR4302536.911088/1‑94 (MQ=60)
                           GCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATA                                                                   <  SRR4302536.1836763/94‑1 (MQ=60)
                            CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  >  SRR4302536.2769191/1‑94 (MQ=60)
                             AGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGA                                                                 >  SRR4302536.3272327/1‑94 (MQ=60)
                             AGTCGAACCTGAAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGC                                                                 >  SRR4302536.3446744/1‑94 (MQ=60)
                              GTCGAACCTGCAGGCGCAATTCTTCGGCACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                <  SRR4302536.2399010/94‑1 (MQ=60)
                               TCGAGCCTGCAGTCTCAATCCTTCGTGGCGCGCTAGCGGCACATAACGCTGCCGCAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGT                                                               <  SRR4302536.3292389/94‑1 (MQ=44)
                               TCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCTGTATAGAGT                                                               <  SRR4302536.6806855/94‑1 (MQ=60)
                                CGACCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              <  SRR4302536.4681652/94‑1 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              >  SRR4302536.3856691/1‑94 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              <  SRR4302536.3252445/94‑1 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              >  SRR4302536.2345247/1‑94 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              <  SRR4302536.36100/94‑1 (MQ=60)
                                 GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGA                                                             <  SRR4302536.1825516/94‑1 (MQ=60)
                                 GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGA                                                             <  SRR4302536.162422/94‑1 (MQ=60)
                                   ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           <  SRR4302536.303054/94‑1 (MQ=60)
                                     CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         >  SRR4302536.6514585/1‑94 (MQ=60)
                                     CTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC                                                         >  SRR4302536.3680058/1‑94 (MQ=60)
                                       GCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAC                                                       <  SRR4302536.3637407/94‑1 (MQ=60)
                                       GCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAC                                                       <  SRR4302536.3250481/94‑1 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      >  SRR4302536.3542817/1‑94 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      >  SRR4302536.6925367/1‑94 (MQ=60)
                                         GGGCGCAATGGTTCGTGACGCCCAAGCGGGACATAACGCTGCCACGGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGA                                                     <  SRR4302536.3376014/94‑1 (MQ=60)
                                         AGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGA                                                     >  SRR4302536.3717231/1‑94 (MQ=60)
                                         AGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGA                                                     <  SRR4302536.4797904/94‑1 (MQ=60)
                                          GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    <  SRR4302536.3877037/94‑1 (MQ=60)
                                          GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    <  SRR4302536.2091396/94‑1 (MQ=60)
                                           GCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATA                                                   <  SRR4302536.5769625/94‑1 (MQ=60)
                                           GCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATA                                                   <  SRR4302536.2087132/94‑1 (MQ=60)
                                            CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302536.2380015/94‑1 (MQ=60)
                                             GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 <  SRR4302536.1844895/94‑1 (MQ=60)
                                              CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302536.6370863/94‑1 (MQ=60)
                                              CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                >  SRR4302536.1595029/1‑94 (MQ=60)
                                              CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302536.5127605/94‑1 (MQ=60)
                                               AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAGACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               >  SRR4302536.4520259/1‑94 (MQ=60)
                                               AATGCTTCGTGACGCAACAGCGGGACATACCGCTGCAACAGCGAGAGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATACACA                                               <  SRR4302536.1740286/94‑1 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              >  SRR4302536.2610892/1‑94 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              >  SRR4302536.1339893/1‑94 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              >  SRR4302536.2402598/1‑94 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             >  SRR4302536.5715322/1‑94 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             >  SRR4302536.6181128/1‑94 (MQ=60)
                                                  tcttggtcacggACCAGCGGGAAATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                            <  SRR4302536.36942/82‑1 (MQ=60)
                                                   CTTCGTTACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGGATAGAGTGAATTCACGATAAACAGCCC                                           >  SRR4302536.4021521/1‑94 (MQ=60)
                                                   CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                           <  SRR4302536.6554461/94‑1 (MQ=60)
                                                   CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                           <  SRR4302536.848117/94‑1 (MQ=60)
                                                     TCGTGACGCCCCAGCGAGACATAACGCGGCCACAGCGATCGTTTATCGATTACACCTTCAGCGGTATAGAGTGAATTCACGTTAATCAGCCCTG                                         <  SRR4302536.3650889/94‑1 (MQ=60)
                                                     TCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTG                                         <  SRR4302536.83808/94‑1 (MQ=60)
                                                      CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGC                                        >  SRR4302536.2387144/1‑94 (MQ=60)
                                                      CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGC                                        >  SRR4302536.6744896/1‑94 (MQ=60)
                                                       GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       <  SRR4302536.2358621/94‑1 (MQ=60)
                                                       GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       >  SRR4302536.6612269/1‑94 (MQ=60)
                                                        TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302536.1318462/1‑94 (MQ=60)
                                                        TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302536.3953076/1‑94 (MQ=60)
                                                        TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302536.4150512/1‑94 (MQ=60)
                                                         GACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTT                                     <  SRR4302536.694658/94‑1 (MQ=60)
                                                         GACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTT                                     <  SRR4302536.837308/94‑1 (MQ=60)
                                                         GACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTT                                     <  SRR4302536.4747083/94‑1 (MQ=60)
                                                          ACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTA                                    <  SRR4302536.6066310/94‑1 (MQ=60)
                                                              ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                                >  SRR4302536.6384595/1‑94 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              >  SRR4302536.3518542/1‑94 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATTCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGGCCTGCGTTATATGAGT                             >  SRR4302536.3149309/1‑94 (MQ=60)
                                                                  GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302536.1394101/94‑1 (MQ=60)
                                                                  GCGGGACATAACGCTGCCACAGCGACCGTTCATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302536.2532953/94‑1 (MQ=60)
                                                                   CGGGACATAGCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTA                           <  SRR4302536.6431414/94‑1 (MQ=60)
                                                                   CGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTA                           <  SRR4302536.6360288/94‑1 (MQ=60)
                                                                    GGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAT                          >  SRR4302536.378888/1‑94 (MQ=60)
                                                                      GACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCG                        <  SRR4302536.372605/94‑1 (MQ=60)
                                                                       ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       <  SRR4302536.2247775/94‑1 (MQ=60)
                                                                         ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     <  SRR4302536.2284183/94‑1 (MQ=60)
                                                                         ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTGCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     <  SRR4302536.4336025/94‑1 (MQ=60)
                                                                          TAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAT                    >  SRR4302536.3598066/1‑94 (MQ=60)
                                                                          TAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAT                    <  SRR4302536.4394083/94‑1 (MQ=60)
                                                                           AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302536.1136305/1‑94 (MQ=60)
                                                                           AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302536.6001322/1‑94 (MQ=60)
                                                                             CGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCTCTGCGTTATATGAGTTATCGGCATGAT                 >  SRR4302536.5512903/1‑94 (MQ=60)
                                                                             CGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAT                 <  SRR4302536.3872019/94‑1 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                >  SRR4302536.628678/1‑94 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                >  SRR4302536.2942698/1‑94 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302536.97914/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302536.5350458/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              >  SRR4302536.4964074/1‑94 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              >  SRR4302536.3973269/1‑94 (MQ=60)
                                                                                 GCCACAGCGAGCGTTCATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATC             <  SRR4302536.5451289/94‑1 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            <  SRR4302536.4673765/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           <  SRR4302536.1267250/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           <  SRR4302536.5400685/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           <  SRR4302536.140007/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302536.1748368/1‑94 (MQ=60)
                                                                                     CAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTT         <  SRR4302536.6834893/94‑1 (MQ=60)
                                                                                     CAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTT         <  SRR4302536.3145929/94‑1 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        >  SRR4302536.3818574/1‑94 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        <  SRR4302536.1130933/94‑1 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        <  SRR4302536.1917517/94‑1 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        <  SRR4302536.2578177/94‑1 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        >  SRR4302536.3818573/1‑94 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        >  SRR4302536.3877867/1‑94 (MQ=60)
                                                                                      AGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTT        >  SRR4302536.539914/1‑94 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302536.5088052/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302536.5650560/94‑1 (MQ=60)
                                                                                         GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG     <  SRR4302536.2153894/94‑1 (MQ=60)
                                                                                          AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    >  SRR4302536.302137/1‑94 (MQ=60)
                                                                                          AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    >  SRR4302536.2472324/1‑94 (MQ=60)
                                                                                          AGCGTTTATCCATTACACCTTCAGCGGGATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    >  SRR4302536.5930891/1‑94 (MQ=60)
                                                                                            CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  SRR4302536.4632072/1‑94 (MQ=60)
                                                                                            CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  <  SRR4302536.5679106/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999680‑1999865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑