Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,088,609 T→C P132P (CCT→CCC)  hisG → ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,088,6090TC97.9% 437.7 / ‑0.4 140P132P (CCT→CCC) hisGATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (66/71);  total (68/72)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 146 total aligned reads displayed.

GGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088520‑2088698
                                                                                         |                                                                                         
ggcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAc                                                                                        >  1:1612435/1‑93 (MQ=255)
 gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       >  1:1130286/1‑93 (MQ=255)
 gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       <  1:2871362/93‑1 (MQ=255)
 gcTGTCGTCTTGCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAGCGGTGAGCGTATCGCCACCTCTTATCCCCAcc                                                                                       <  2:3048250/93‑1 (MQ=255)
  cTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCt                                                                                      >  2:1731933/1‑93 (MQ=255)
   tGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTg                                                                                     >  2:3070661/1‑93 (MQ=255)
    gtgctcTTTCGCCGGCAACGCGGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGc                                                                                    <  2:1342587/89‑1 (MQ=255)
    gtcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCCGc                                                                                    >  1:1114851/1‑93 (MQ=255)
    gtcgacTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGTCACCTCTTATCACCACCTgt                                                                                    >  2:928780/1‑92 (MQ=255)
     tcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCt                                                                                   >  1:3154291/1‑93 (MQ=255)
       gtcTTTCGCTGGCAACGCCGGTTGAAGAAGCCTGGGACGGTCCGCTATCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCa                                                                                 <  1:2515410/93‑1 (MQ=255)
        tcTTTCGCTGGCAACGCCGGTTTATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCaa                                                                                <  1:664135/93‑1 (MQ=255)
          tttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGc                                                                              <  1:2041186/93‑1 (MQ=255)
            tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  2:1726971/93‑1 (MQ=255)
            tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  2:1365273/93‑1 (MQ=255)
               cTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAt                                                                         <  1:90023/93‑1 (MQ=255)
                tGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtt                                                                        >  2:3580956/1‑92 (MQ=255)
                tGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtc                                                                        <  2:661031/93‑1 (MQ=255)
                tGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtc                                                                        >  2:1968610/1‑93 (MQ=255)
                 ggCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtct                                                                       >  2:347987/1‑93 (MQ=255)
                  gCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtctc                                                                      <  1:2426205/93‑1 (MQ=255)
                  gCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtctc                                                                      <  2:520841/93‑1 (MQ=255)
                     aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   >  1:497875/1‑93 (MQ=255)
                     aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   >  1:1340954/1‑93 (MQ=255)
                       gCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCa                                                                 <  1:2503960/93‑1 (MQ=255)
                       gCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCa                                                                 <  1:2140321/93‑1 (MQ=255)
                         cGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCCGa                                                               >  1:1835169/1‑93 (MQ=255)
                          ggTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaa                                                              >  2:2744615/1‑93 (MQ=255)
                          ggTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaa                                                              <  2:2718018/93‑1 (MQ=255)
                            ttGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAg                                                            <  2:3965806/93‑1 (MQ=255)
                              gatgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGc                                                          <  1:1162631/93‑1 (MQ=255)
                              gatgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGCTATCTCGACCAGAAAGGc                                                          >  1:4180843/1‑93 (MQ=255)
                               atgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCa                                                         <  2:2431657/93‑1 (MQ=255)
                                tgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAGCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAt                                                        <  1:135184/93‑1 (MQ=255)
                                 gaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtc                                                       <  1:2745517/93‑1 (MQ=255)
                                   aGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtctc                                                     >  1:1494288/1‑93 (MQ=255)
                                    gCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATctct                                                    <  1:2994217/93‑1 (MQ=255)
                                     ccTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtt                                                   <  2:1881427/93‑1 (MQ=255)
                                       tGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtttt                                                 <  2:1178591/93‑1 (MQ=255)
                                       tGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtttt                                                 >  1:836368/1‑93 (MQ=255)
                                           aCGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAt                                             <  2:94450/93‑1 (MQ=255)
                                            cGGTCCGCACTCCTTAAACGGAAAACGTATCGCCACCTCTTAACCCCACCTGCACAAGCGTTAACTCGACCAGAAAGGAATCTCTTTTAAATc                                            <  2:4356793/93‑1 (MQ=255)
                                             ggTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATcc                                           >  2:4551773/1‑93 (MQ=255)
                                             ggTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATcc                                           <  1:2697828/93‑1 (MQ=255)
                                              gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  1:3254851/93‑1 (MQ=255)
                                              gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  2:4656897/93‑1 (MQ=255)
                                              gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  1:294909/93‑1 (MQ=255)
                                              gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          >  1:210678/1‑93 (MQ=255)
                                               tCCGCTCTCCTTAAACGGTAAACGTATCTCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCtc                                         >  1:2753952/1‑92 (MQ=255)
                                                ccGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGc                                        >  1:187385/1‑93 (MQ=255)
                                                ccGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGc                                        >  1:69751/1‑93 (MQ=255)
                                                 cGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCt                                       >  2:3981169/1‑93 (MQ=255)
                                                 cGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCt                                       >  2:3107371/1‑93 (MQ=255)
                                                 cGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCt                                       >  1:737390/1‑93 (MQ=255)
                                                   ccctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     <  2:3420836/91‑1 (MQ=255)
                                                    tctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAc                                    >  2:1250281/1‑93 (MQ=255)
                                                    tctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAc                                    >  1:1660622/1‑93 (MQ=255)
                                                      tcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTg                                  >  1:3883013/1‑93 (MQ=255)
                                                      tcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTg                                  <  2:2455516/93‑1 (MQ=255)
                                                       ccTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGa                                 >  1:4325878/1‑93 (MQ=255)
                                                       ccTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGa                                 <  1:326695/93‑1 (MQ=255)
                                                       ccTTAAACGGTAAACGTATCGCCACCTCATATCCCCACCTGCTCACGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGa                                 <  1:4221532/93‑1 (MQ=255)
                                                        cTTAAACGGTAAACGTATCGCCAGCTCTTATTCCCACCTGCGCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGac                                >  2:3080681/1‑92 (MQ=255)
                                                        cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                <  1:4428799/93‑1 (MQ=255)
                                                        cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                >  2:2293086/1‑93 (MQ=255)
                                                        cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                <  2:1518168/93‑1 (MQ=255)
                                                        cTTAAACGGTAAACGTATCGCCACCTCTTATCCCAACCTCCTCAAGCGTTATTTCGACAGGAAAGGAACTTCTTTTAATTCTTCCTTCCTGac                                >  2:1834032/1‑92 (MQ=255)
                                                         ttAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAc                               >  1:601676/1‑93 (MQ=255)
                                                         ttAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAc                               <  2:3217141/93‑1 (MQ=255)
                                                          tAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  2:4193710/1‑93 (MQ=255)
                                                           aaaCGGTAATCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACgg                             <  2:141985/93‑1 (MQ=255)
                                                            aaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGt                            >  2:2184068/1‑93 (MQ=255)
                                                            aaCGGTAAACGTATCGCCACCTCTTACCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGt                            <  1:40619/93‑1 (MQ=255)
                                                             aCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGtt                           >  2:3934334/1‑93 (MQ=255)
                                                              cGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTc                          <  1:242734/93‑1 (MQ=255)
                                                               ggTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCt                         <  1:2198422/93‑1 (MQ=255)
                                                               ggTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCt                         <  1:4310436/93‑1 (MQ=255)
                                                                gTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTg                        >  1:3879339/1‑93 (MQ=255)
                                                                 tAAACGTATCGCCACCTCTTCACCCCACCTGCACAAGCGTAATCTCGACCAGAAAGGAATCTCTATTAAATACAACTTACAAAACGGTTATga                       <  2:3596811/93‑2 (MQ=255)
                                                                 tAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGt                       <  1:3490572/93‑1 (MQ=255)
                                                                     cGTATCGCCACCTCTTAGCCCCTCCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGaa                   <  2:2364571/93‑1 (MQ=255)
                                                                      gTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAg                  >  1:2643690/1‑93 (MQ=255)
                                                                       tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGt                 >  1:1422505/1‑93 (MQ=255)
                                                                       tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGt                 <  1:4683909/93‑1 (MQ=255)
                                                                       tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGt                 <  1:4624299/93‑1 (MQ=255)
                                                                       tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGGTGAAGt                 >  2:4141193/1‑93 (MQ=255)
                                                                        acccccACCTCCTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTc                <  2:1259482/89‑1 (MQ=255)
                                                                        aTCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTc                >  1:4669268/1‑93 (MQ=255)
                                                                         tCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCg               <  2:3366428/93‑1 (MQ=255)
                                                                             cACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcccc           >  1:814035/1‑93 (MQ=255)
                                                                               ccTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc         >  2:411218/1‑93 (MQ=255)
                                                                               ccTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc         >  1:2852440/1‑93 (MQ=255)
                                                                               ccTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc         <  1:2048972/93‑1 (MQ=255)
                                                                                 tctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGt       <  2:1718480/93‑1 (MQ=255)
                                                                                 tctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGt       >  2:1799405/1‑93 (MQ=255)
                                                                                 tctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGt       <  1:3110656/93‑1 (MQ=255)
                                                                                  ctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTCCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTg      <  2:2682720/93‑1 (MQ=255)
                                                                                    tATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGcc    <  1:3318535/93‑1 (MQ=255)
                                                                                    tATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGcc    >  2:218679/1‑93 (MQ=255)
                                                                                      tCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg  >  1:308310/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088520‑2088698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088517‑2088702
                                                                                            |                                                                                             
GGCCGCTGTCGTCTTTCGCTGGCAGCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCC                                                                                              <  SRR4302544.4072769/94‑1 (MQ=60)
  CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAC                                                                                            >  SRR4302544.1628331/1‑94 (MQ=60)
  CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAC                                                                                            >  SRR4302544.1628332/1‑94 (MQ=60)
   GGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACC                                                                                           >  SRR4302544.1141549/1‑94 (MQ=60)
    GCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCT                                                                                          <  SRR4302544.2899078/94‑1 (MQ=60)
    GCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCT                                                                                          <  SRR4302544.1989894/94‑1 (MQ=60)
      TGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCCGC                                                                                        >  SRR4302544.1125977/1‑94 (MQ=60)
       GTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCT                                                                                       >  SRR4302544.3184293/1‑94 (MQ=60)
          GTCTTTCGCTGGCAACGCCGGTTGAAGAAGCCTGGGACGGTCCGCTATCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAA                                                                                    <  SRR4302544.2539566/94‑1 (MQ=60)
           TCTTTCGCTGGCAACGCCGGTTTATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAG                                                                                   <  SRR4302544.670693/94‑1 (MQ=60)
            CTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGC                                                                                  <  SRR4302544.2149335/94‑1 (MQ=60)
             TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCG                                                                                 >  SRR4302544.4317673/1‑94 (MQ=60)
             TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCG                                                                                 <  SRR4302544.2060885/94‑1 (MQ=60)
                  CTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATC                                                                            <  SRR4302544.90870/94‑1 (MQ=60)
                     GCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCG                                                                         <  SRR4302544.2449297/94‑1 (MQ=60)
                       AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       >  SRR4302544.502765/1‑94 (MQ=60)
                       AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       >  SRR4302544.1354210/1‑94 (MQ=60)
                          GCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAG                                                                    <  SRR4302544.863825/94‑1 (MQ=60)
                          GCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAG                                                                    <  SRR4302544.4676265/94‑1 (MQ=60)
                          GCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAG                                                                    <  SRR4302544.2160752/94‑1 (MQ=60)
                          GCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAG                                                                    <  SRR4302544.2527965/94‑1 (MQ=60)
                           CCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCCGA                                                                   >  SRR4302544.1852990/1‑94 (MQ=60)
                                TGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGC                                                              >  SRR4302544.2761824/1‑94 (MQ=60)
                                TGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGCTATCTCGACCAGAAAGGC                                                              >  SRR4302544.4222247/1‑94 (MQ=60)
                                 GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCA                                                             <  SRR4302544.1174220/94‑1 (MQ=60)
                                   TGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAGCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATC                                                           <  SRR4302544.136457/94‑1 (MQ=60)
                                    GAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCT                                                          <  SRR4302544.2771969/94‑1 (MQ=60)
                                     AAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTC                                                         >  SRR4302544.1509065/1‑94 (MQ=60)
                                       GCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTT                                                       <  SRR4302544.3530037/94‑1 (MQ=60)
                                       GCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTT                                                       <  SRR4302544.3022924/94‑1 (MQ=60)
                                         CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTT                                                     >  SRR4302544.844609/1‑94 (MQ=60)
                                         CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTT                                                     >  SRR4302544.4785911/1‑94 (MQ=60)
                                           GGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAA                                                   <  SRR4302544.1277719/94‑1 (MQ=60)
                                             GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 >  SRR4302544.2490571/1‑94 (MQ=60)
                                              GCGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATC                                                <  SRR4302544.627913/94‑1 (MQ=60)
                                               CGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCC                                               <  SRR4302544.2400595/94‑1 (MQ=60)
                                               CGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCC                                               <  SRR4302544.3263285/94‑1 (MQ=60)
                                                GGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCT                                              <  SRR4302544.2723744/94‑1 (MQ=60)
                                                GGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCT                                              >  SRR4302544.212849/1‑94 (MQ=60)
                                                 GTCCGCTCTCCTTAAACGGTAAACGTATCTCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTC                                             >  SRR4302544.2780463/1‑94 (MQ=60)
                                                 GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             <  SRR4302544.297956/94‑1 (MQ=60)
                                                 GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             <  SRR4302544.3285974/94‑1 (MQ=60)
                                                 GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTC                                             >  SRR4302544.2780464/1‑94 (MQ=60)
                                                  TCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGC                                            >  SRR4302544.70434/1‑94 (MQ=60)
                                                  TCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGC                                            >  SRR4302544.189255/1‑94 (MQ=60)
                                                   CCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCT                                           >  SRR4302544.744601/1‑94 (MQ=60)
                                                      CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        >  SRR4302544.1676977/1‑94 (MQ=60)
                                                      CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        >  SRR4302544.3314218/1‑94 (MQ=60)
                                                        CTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTG                                      >  SRR4302544.3921582/1‑94 (MQ=60)
                                                         TCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGA                                     <  SRR4302544.4444087/94‑1 (MQ=60)
                                                         TCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGA                                     >  SRR4302544.4369929/1‑94 (MQ=60)
                                                          CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    <  SRR4302544.4026301/94‑1 (MQ=60)
                                                          CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    <  SRR4302544.330096/94‑1 (MQ=60)
                                                          CCTTAAACGGTAAACGTATCGCCACCTCATATCCCCACCTGCTCACGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    <  SRR4302544.4264347/94‑1 (MQ=60)
                                                           CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   <  SRR4302544.4473485/94‑1 (MQ=60)
                                                           CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   >  SRR4302544.607575/1‑94 (MQ=60)
                                                               AACGGTAAACGTATCGCCACCTCTTACCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTT                               <  SRR4302544.41024/94‑1 (MQ=60)
                                                                 CGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCT                             <  SRR4302544.245257/94‑1 (MQ=60)
                                                                  GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            >  SRR4302544.3917858/1‑94 (MQ=60)
                                                                  GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            <  SRR4302544.2219143/94‑1 (MQ=60)
                                                                  GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            <  SRR4302544.4354367/94‑1 (MQ=60)
                                                                   GTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGT                           >  SRR4302544.1732643/1‑94 (MQ=60)
                                                                    TAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTT                          <  SRR4302544.3523847/94‑1 (MQ=60)
                                                                        CGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAG                      >  SRR4302544.2669037/1‑94 (MQ=60)
                                                                         GTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGT                     >  SRR4302544.3135034/1‑94 (MQ=60)
                                                                         GTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGT                     >  SRR4302544.1436660/1‑94 (MQ=60)
                                                                          TATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTC                    <  SRR4302544.4670169/94‑1 (MQ=60)
                                                                          TATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTC                    >  SRR4302544.4715428/1‑94 (MQ=60)
                                                                          TATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTC                    <  SRR4302544.4730209/94‑1 (MQ=60)
                                                                               CCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCC               >  SRR4302544.821981/1‑94 (MQ=60)
                                                                                CACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCG              <  SRR4302544.3016533/94‑1 (MQ=60)
                                                                                 ACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC             >  SRR4302544.2880000/1‑94 (MQ=60)
                                                                                  CCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCG            <  SRR4302544.2068709/94‑1 (MQ=60)
                                                                                   CTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGT           >  SRR4302544.707431/1‑94 (MQ=60)
                                                                                    TCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTG          <  SRR4302544.3140253/94‑1 (MQ=60)
                                                                                     CTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGC         >  SRR4302544.1377757/1‑94 (MQ=60)
                                                                                      TTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCC        <  SRR4302544.2630475/94‑1 (MQ=60)
                                                                                       TATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCG       <  SRR4302544.3350149/94‑1 (MQ=60)
                                                                                        ATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG      >  SRR4302544.311511/1‑94 (MQ=60)
                                                                                           CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302544.2699211/94‑1 (MQ=60)
                                                                                           CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302544.747834/94‑1 (MQ=60)
                                                                                           CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302544.1723902/94‑1 (MQ=60)
                                                                                           CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302544.3169290/94‑1 (MQ=60)
                                                                                            CCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  SRR4302544.1543331/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088517‑2088702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑