Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108,194	T→C	Q283R (CAG→CGG)	rfbA ←	glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108,194	0	T	C	99.4%	583.7 / ‑4.9	178	Q283R (CAG→CGG)	rfbA	glucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (84/93); total (84/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 181 total aligned reads displayed.

AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283

    aCTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCAt                                                                                     <  1:4209791/93‑1 (MQ=255)

          ggCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattat                                                                               >  1:2958602/1‑93 (MQ=255)

          ggCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattat                                                                               <  1:2474649/93‑1 (MQ=255)

           gCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAattatt                                                                              <  2:3684409/93‑1 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  2:4533797/93‑1 (MQ=255)

             tCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            >  2:132763/1‑93 (MQ=255)

             tCCAGAATTAGCACACCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCt                                                                            <  1:844233/93‑1 (MQ=255)

               cAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCttt                                                                          >  2:3222992/1‑93 (MQ=255)

               cAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCttt                                                                          >  1:1296663/1‑93 (MQ=255)

                aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         <  2:377484/93‑1 (MQ=255)

                aGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTtta                                                                         >  2:2212879/1‑93 (MQ=255)

                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  1:2557950/1‑93 (MQ=255)

                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:4265550/1‑93 (MQ=255)

                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  1:2967190/1‑93 (MQ=255)

                 gAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttat                                                                        >  2:1987430/1‑93 (MQ=255)

                  aaTTAGCACATCTTCAATTTCCGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  1:4230243/1‑93 (MQ=255)

                  aaTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatt                                                                       >  2:1816442/1‑93 (MQ=255)

                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      <  2:3393594/93‑1 (MQ=255)

                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      >  1:516042/1‑93 (MQ=255)

                   aTTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTttatta                                                                      <  1:4699907/93‑1 (MQ=255)

                     tAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGt                                                                    <  1:3636856/93‑1 (MQ=255)

                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   >  2:721600/1‑93 (MQ=255)

                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   >  2:1373499/1‑93 (MQ=255)

                      aGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTg                                                                   <  2:4312624/93‑1 (MQ=255)

                        cacaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 <  1:3566885/93‑1 (MQ=255)

                        cacaTCTTCAATTTCAGTGCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGt                                                                 <  2:3005273/93‑1 (MQ=255)

                         acaTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTa                                                                <  1:3403670/93‑1 (MQ=255)

                           ctCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              <  2:2138808/92‑1 (MQ=255)

                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              <  1:2948377/93‑1 (MQ=255)

                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              >  2:3769928/1‑93 (MQ=255)

                           aTCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACa                                                              >  1:3689216/1‑93 (MQ=255)

                            tCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAg                                                             >  2:2688950/1‑93 (MQ=255)

                            tCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAg                                                             >  2:4157192/1‑93 (MQ=255)

                               tCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTa                                                          >  2:2651494/1‑93 (MQ=255)

                                cAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTaa                                                         <  1:2095797/93‑1 (MQ=255)

                                  aTTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAtt                                                       >  2:1093439/1‑93 (MQ=255)

                                   tttCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAttt                                                      >  1:1175082/1‑93 (MQ=255)

                                       aGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCtt                                                  <  2:69111/93‑1 (MQ=255)

                                        gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 >  2:1348568/1‑93 (MQ=255)

                                        gTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTa                                                 <  2:644832/93‑1 (MQ=255)

                                         ttCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTAc                                                >  2:3620606/1‑93 (MQ=255)

                                          tCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACt                                               <  2:3311895/93‑1 (MQ=255)

                                           cTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACtt                                              <  2:2952149/93‑1 (MQ=255)

                                             aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            >  1:1346163/1‑93 (MQ=255)

                                             aaTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGc                                            >  1:4357117/1‑93 (MQ=255)

                                              aTCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCt                                           <  2:4140100/93‑1 (MQ=255)

                                               tCACATTCGTTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAGTTGTTCTTTATTACTGGTACAGCTAATGTTCTTACTTGCtt                                          >  2:1926974/1‑92 (MQ=255)

                                               tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  2:1054223/1‑93 (MQ=255)

                                               tCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTc                                          >  2:1205779/1‑93 (MQ=255)

                                                 acaTTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCaa                                        >  2:3193035/1‑93 (MQ=255)

                                                  cattcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAAc                                       <  1:484971/93‑1 (MQ=255)

                                                    ttcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     <  2:4050456/93‑1 (MQ=255)

                                                    ttcattAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAt                                     >  1:1488776/1‑93 (MQ=255)

                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:894315/1‑93 (MQ=255)

                                                       attAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCaa                                  >  1:894318/1‑93 (MQ=255)

                                                           aTTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaa                              >  1:2676806/1‑93 (MQ=255)

                                                            tttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATaaaa                             >  2:3464026/1‑93 (MQ=255)

                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  2:1471304/93‑1 (MQ=255)

                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  1:159482/93‑1 (MQ=255)

                                                             ttGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAAc                            <  2:3211957/93‑1 (MQ=255)

                                                               gAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCt                          <  1:3271499/93‑1 (MQ=255)

                                                               gAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCt                          <  2:4726060/93‑1 (MQ=255)

                                                                aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         >  1:4693113/1‑93 (MQ=255)

                                                                aaTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCtt                         <  2:237773/93‑1 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  2:3881247/93‑1 (MQ=255)

                                                                 aTCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCttt                        <  1:4536662/93‑1 (MQ=255)

                                                                  tCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTa                       >  1:1165702/1‑93 (MQ=255)

                                                                   ccTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAc                      >  1:3105266/1‑93 (MQ=255)

                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:2224216/1‑93 (MQ=255)

                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:1684535/1‑93 (MQ=255)

                                                                    cTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACg                     >  2:2993093/1‑93 (MQ=255)

                                                                     ttCGTCATTTTATAAAGATACCGCCCCTAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGa                    >  2:3846548/1‑93 (MQ=255)

                                                                      tCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaa                   >  1:4610920/1‑93 (MQ=255)

                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:3798888/93‑1 (MQ=255)

                                                                       cGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGaaa                  <  1:3038918/93‑1 (MQ=255)

                                                                        gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 <  2:2836986/93‑1 (MQ=255)

                                                                        gTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAt                 >  1:288187/1‑93 (MQ=255)

                                                                         tCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTACTTTTCTTACTTGCTCAACATCAATAAACCCTTTACGAAATg                >  1:3627344/1‑93 (MQ=255)

                                                                          cATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGc               <  1:2688950/93‑1 (MQ=255)

                                                                          cATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGc               >  2:3496988/1‑93 (MQ=255)

                                                                             tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            <  2:867390/93‑1 (MQ=255)

                                                                             tttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAt            >  1:3456947/1‑93 (MQ=255)

                                                                              ttATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtc           <  2:2078905/93‑1 (MQ=255)

                                                                               tataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtct          <  1:4331100/93‑1 (MQ=255)

                                                                               tataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtct          >  1:1895752/1‑93 (MQ=255)

                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         >  1:3776467/1‑93 (MQ=255)

                                                                                ataAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAtctc         >  1:3354020/1‑93 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:3065095/93‑1 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  2:245225/93‑1 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  1:316255/93‑1 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        >  1:1320314/1‑93 (MQ=255)

                                                                                 taAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATctct        <  1:1206385/93‑1 (MQ=255)

                                                                                  aaaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCtt       <  1:2058850/93‑1 (MQ=255)

                                                                                  aaaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCtt       <  1:3578964/93‑1 (MQ=255)

                                                                                   aaGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTc      >  1:2039603/1‑93 (MQ=255)

                                                                                    aGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTca     <  2:2535603/93‑1 (MQ=255)

                                                                                     gATACCGCCCAGAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    >  2:4679156/1‑93 (MQ=255)

                                                                                     aaTACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcag    <  1:1627639/92‑1 (MQ=255)

                                                                                      aTACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagg   <  2:1829025/93‑1 (MQ=255)

                                                                                       tACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTcagga  <  1:3151022/93‑1 (MQ=255)

AAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA  >  NC_000913/2108104‑2108283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAG  >  NC_000913/2108102‑2108286

CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCG                                                                                             <  SRR4302544.581800/94‑1 (MQ=60)

 CAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGC                                                                                            >  SRR4302544.1202740/1‑94 (MQ=60)

 CAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGC                                                                                            >  SRR4302544.1356325/1‑94 (MQ=60)

    ATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCA                                                                                         >  SRR4302544.3572576/1‑94 (MQ=60)

      ACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATA                                                                                       <  SRR4302544.4252130/94‑1 (MQ=60)

         CTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATT                                                                                    >  SRR4302544.4379630/1‑94 (MQ=60)

           TGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTAT                                                                                  >  SRR4302544.123204/1‑94 (MQ=60)

           TGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTAT                                                                                  >  SRR4302544.2987005/1‑94 (MQ=60)

            GGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATT                                                                                 <  SRR4302544.2498310/94‑1 (MQ=60)

              CTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCT                                                                               >  SRR4302544.2959416/1‑94 (MQ=60)

               TCCAGAATTAGCACACCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTT                                                                              <  SRR4302544.852567/94‑1 (MQ=60)

                CCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTT                                                                             >  SRR4302544.1309431/1‑94 (MQ=60)

                CCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAANTTGAATCCTTCGTCATTTTATAANNNNACNNCCCATANTNATTCNNN                                                                             >  SRR4302544.3881239/1‑94 (MQ=60)

                  AGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTAT                                                                           >  SRR4302544.2995652/1‑94 (MQ=60)

                  AGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTAT                                                                           >  SRR4302544.2582514/1‑94 (MQ=60)

                  AGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTAT                                                                           >  SRR4302544.2995654/1‑94 (MQ=60)

                   GAATTAGCACATCTTCAATTTCCGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATT                                                                          >  SRR4302544.4273345/1‑94 (MQ=60)

                    AATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTA                                                                         >  SRR4302544.861901/1‑94 (MQ=60)

                    AATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTA                                                                         >  SRR4302544.521144/1‑94 (MQ=60)

                     ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                        <  SRR4302544.1698392/94‑1 (MQ=60)

                     ATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAG                                                                        <  SRR4302544.4746320/94‑1 (MQ=60)

                       TAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTG                                                                      <  SRR4302544.3673131/94‑1 (MQ=60)

                        AGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCTTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGG                                                                     >  SRR4302544.2210175/1‑94 (MQ=60)

                          CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCTATAATTATTCTTTATTAGTGGTA                                                                   <  SRR4302544.646152/94‑1 (MQ=60)

                          CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                   <  SRR4302544.3043766/94‑1 (MQ=60)

                          CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                   <  SRR4302544.3601082/94‑1 (MQ=60)

                          CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                   <  SRR4302544.3601081/94‑1 (MQ=60)

                          CACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTA                                                                   >  SRR4302544.3227203/1‑94 (MQ=60)

                           ACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTAC                                                                  <  SRR4302544.3436212/94‑1 (MQ=60)

                            CATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACA                                                                 >  SRR4302544.3725984/1‑94 (MQ=60)

                             ATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAG                                                                <  SRR4302544.2976696/94‑1 (MQ=60)

                               CTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCT                                                              <  SRR4302544.1611127/94‑1 (MQ=60)

                                  CAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAT                                                           <  SRR4302544.2115966/94‑1 (MQ=60)

                                  CAATGTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAAT                                                           <  SRR4302544.2115965/94‑1 (MQ=60)

                                    ATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTT                                                         >  SRR4302544.1186799/1‑94 (MQ=60)

                                     TTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTT                                                        <  SRR4302544.267158/94‑1 (MQ=60)

                                            TCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTT                                                 >  SRR4302544.2977316/1‑94 (MQ=60)

                                              TAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGC                                               >  SRR4302544.4401350/1‑94 (MQ=60)

                                              TAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGC                                               >  SRR4302544.1359486/1‑94 (MQ=60)

                                                  CACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAA                                           >  SRR4302544.3325777/1‑94 (MQ=60)

                                                    CATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACA                                         <  SRR4302544.2512356/94‑1 (MQ=60)

                                                    CATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACA                                         <  SRR4302544.489719/94‑1 (MQ=60)

                                                    CATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTATTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACA                                         >  SRR4302544.1323366/1‑94 (MQ=60)

                                                     ATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACAT                                        >  SRR4302544.1503494/1‑94 (MQ=60)

                                                        CATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAA                                     >  SRR4302544.903165/1‑94 (MQ=60)

                                                        CATTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAA                                     >  SRR4302544.903162/1‑94 (MQ=60)

                                                          TTAATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATA                                   <  SRR4302544.4135888/94‑1 (MQ=60)

                                                            AATTTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAA                                 >  SRR4302544.2702462/1‑94 (MQ=60)

                                                               TTGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACC                              <  SRR4302544.161041/94‑1 (MQ=60)

                                                                TGAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCT                             <  SRR4302544.970831/94‑1 (MQ=60)

                                                                 GAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTT                            <  SRR4302544.3302700/94‑1 (MQ=60)

                                                                 GAATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTT                            >  SRR4302544.4739463/1‑94 (MQ=60)

                                                                  AATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTT                           <  SRR4302544.3823537/94‑1 (MQ=60)

                                                                   ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTA                          <  SRR4302544.4582020/94‑1 (MQ=60)

                                                                   ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTA                          <  SRR4302544.1602436/94‑1 (MQ=60)

                                                                   ATCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTA                          >  SRR4302544.1177322/1‑94 (MQ=60)

                                                                    TCCTTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTAC                         >  SRR4302544.3134817/1‑94 (MQ=60)

                                                                       TTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAA                      >  SRR4302544.4656624/1‑94 (MQ=60)

                                                                       TTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAA                      >  SRR4302544.508325/1‑94 (MQ=60)

                                                                       TTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAA                      >  SRR4302544.4656711/1‑94 (MQ=60)

                                                                       TTCGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAA                      >  SRR4302544.4704023/1‑94 (MQ=60)

                                                                         CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                    <  SRR4302544.3836510/94‑1 (MQ=60)

                                                                         CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                    >  SRR4302544.4753750/1‑94 (MQ=60)

                                                                         CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                    <  SRR4302544.3068001/94‑1 (MQ=60)

                                                                         CGTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAAT                    >  SRR4302544.291178/1‑94 (MQ=60)

                                                                          GTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTACTTTTCTTACTTGCTCAACATCAATAAACCCTTTACGAAATG                   >  SRR4302544.3663480/1‑94 (MQ=60)

                                                                          GTCATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATG                   >  SRR4302544.3663478/1‑94 (MQ=60)

                                                                            CATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCA                 <  SRR4302544.2714737/94‑1 (MQ=60)

                                                                             ATTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAA                <  SRR4302544.432445/94‑1 (MQ=60)

                                                                              TTTTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAAT               >  SRR4302544.3489942/1‑94 (MQ=60)

                                                                                TTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCT             >  SRR4302544.1914071/1‑94 (MQ=60)

                                                                                TTATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCT             <  SRR4302544.4541470/94‑1 (MQ=60)

                                                                                 TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC            >  SRR4302544.3778109/1‑94 (MQ=60)

                                                                                 TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC            >  SRR4302544.3386026/1‑94 (MQ=60)

                                                                                 TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC            <  SRR4302544.4375201/94‑1 (MQ=60)

                                                                                 TATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTC            >  SRR4302544.3813891/1‑94 (MQ=60)

                                                                                  ATAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCT           >  SRR4302544.1333300/1‑94 (MQ=60)

                                                                                   TAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTT          <  SRR4302544.1218335/94‑1 (MQ=60)

                                                                                   TAAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTT          <  SRR4302544.319540/94‑1 (MQ=60)

                                                                                    AAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTC         >  SRR4302544.2059294/1‑94 (MQ=60)

                                                                                    AAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTC         <  SRR4302544.2078653/94‑1 (MQ=60)

                                                                                    AAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTC         <  SRR4302544.36880/94‑1 (MQ=60)

                                                                                    AAAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTC         <  SRR4302544.3613293/94‑1 (MQ=60)

                                                                                     AAGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCA        <  SRR4302544.2786782/94‑1 (MQ=60)

                                                                                     AAGATACCGCCCATAATTAGTCTTGATTAGGGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCA        <  SRR4302544.195694/94‑1 (MQ=60)

                                                                                      AGATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAG       <  SRR4302544.3181442/94‑1 (MQ=60)

                                                                                       AATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGG      <  SRR4302544.1643668/94‑1 (MQ=60)

                                                                                        ATACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGA     >  SRR4302544.3448130/1‑94 (MQ=60)

                                                                                         TACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGAC    <  SRR4302544.3180988/94‑1 (MQ=60)

                                                                                         TACCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGAC    <  SRR4302544.3180989/94‑1 (MQ=60)

                                                                                           CCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAG  <  SRR4302544.923842/94‑1 (MQ=60)

                                                                                           CCGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAG  <  SRR4302544.923843/94‑1 (MQ=60)

CCAAATACTCTTGGCTCCAGAATTAGCACATCTTCAATTTCAGTTCTAATCACATTCATTAATTTGAATCCTTCGTCATTTTATAAAGATACTGCCCATAATTATTCTTTATTAGTGGTACAGCTAATTTTCTTACTTGCTCAACATCAATAAAACCTTTACGAAATGCAATCTCTTCAGGACAG  >  NC_000913/2108102‑2108286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`