Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,109,166 A→G V278A (GTC→GCC)  rfbD ← dTDP‑6‑deoxy‑L‑mannose‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,109,1660AG97.8% 701.7 / 0.7 223V278A (GTC→GCC) rfbDdTDP‑6‑deoxy‑L‑mannose‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base G (109/109);  total (113/110)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 238 total aligned reads displayed.

TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255
                                                                                          |                                                                                         
tCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCaggcaaggca                                                                                         >  1:3608428/1‑93 (MQ=255)
 cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAggcaaggcaa                                                                                        <  1:3669649/93‑1 (MQ=255)
   cGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgc                                                                                      >  1:4520114/1‑93 (MQ=255)
   cGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgc                                                                                      <  1:3269276/93‑1 (MQ=255)
   cGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgc                                                                                      <  1:2225070/93‑1 (MQ=255)
    gAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgcg                                                                                     >  2:2060654/1‑93 (MQ=255)
    gAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgcg                                                                                     <  2:3717806/93‑1 (MQ=255)
      aCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGGGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCa                                                                                   <  1:1009894/93‑1 (MQ=255)
      aCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCGGCCAGTCAGGCAAGGCAAGCGCa                                                                                   >  2:2617165/1‑93 (MQ=255)
       cAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaa                                                                                  >  2:3877381/1‑93 (MQ=255)
       cAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaa                                                                                  <  1:3092305/93‑1 (MQ=255)
         aGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAg                                                                                >  1:147808/1‑93 (MQ=255)
          gATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGt                                                                               >  1:4302171/1‑93 (MQ=255)
            tGCAAAAACTATTAAATTGTTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCAAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:1306796/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:749854/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  2:1033554/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:1622613/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:2986498/93‑1 (MQ=255)
             gCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTct                                                                            <  1:907591/93‑1 (MQ=255)
               aaaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgc                                                                          <  2:1207320/93‑1 (MQ=255)
               aaaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgc                                                                          >  2:3761696/1‑93 (MQ=255)
                aaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgct                                                                         >  2:4547958/1‑93 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  1:2756182/1‑93 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  1:2577064/1‑93 (MQ=255)
                  aaCTATGAAATTGCTGCAGTCGTAAATAATTCATTGAGCATTCGTTTTACGCCAACCTGCCAGTCATGCACGGCAAGAGCAATGTTctgtgaa                                                                       >  2:1574006/1‑89 (MQ=255)
                    cTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa                                                                     >  1:4019752/1‑93 (MQ=255)
                       ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAttt                                                                  <  1:2673269/93‑1 (MQ=255)
                        tAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtttt                                                                 <  2:2709289/93‑1 (MQ=255)
                        tAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtttt                                                                 <  2:3247633/93‑1 (MQ=255)
                         aaaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAttttt                                                                >  2:1344774/1‑93 (MQ=255)
                          aaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTc                                                               >  1:2777046/1‑93 (MQ=255)
                           aTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCt                                                              >  2:560459/1‑93 (MQ=255)
                           aTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCt                                                              <  1:3183622/93‑1 (MQ=255)
                           aTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCt                                                              <  1:2417642/93‑1 (MQ=255)
                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTg                                                             >  2:1467586/1‑93 (MQ=255)
                              gCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTa                                                           <  2:3542082/93‑1 (MQ=255)
                               gcttAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt                                                          >  2:64004/4‑93 (MQ=255)
                               cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt                                                          <  1:126958/93‑1 (MQ=255)
                               cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt                                                          <  2:667965/93‑1 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         <  1:2975148/93‑1 (MQ=255)
                                 gTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTa                                                        >  1:3772302/1‑93 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       >  1:352977/1‑93 (MQ=255)
                                   actCGTAACTAATTCATTGACCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAg                                                      <  2:3968974/91‑1 (MQ=255)
                                     tCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                    <  1:3899000/93‑1 (MQ=255)
                                     tCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                    <  2:3121720/93‑1 (MQ=255)
                                      cGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCg                                                   >  1:4732954/1‑93 (MQ=255)
                                       gTATATAATTCATTGAGCAGTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  <  2:3935829/93‑1 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  >  2:4159062/1‑93 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  <  1:2767600/93‑1 (MQ=255)
                                        tAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgag                                                 >  2:2173658/1‑93 (MQ=255)
                                        tAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgag                                                 >  1:2623466/1‑93 (MQ=255)
                                        tAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgag                                                 >  1:28735/1‑93 (MQ=255)
                                         aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga                                                >  1:4266411/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  2:1361742/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               <  2:2270379/93‑1 (MQ=255)
                                           ataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGt                                              <  2:3695352/93‑1 (MQ=255)
                                            taatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGtt                                             <  1:575032/93‑1 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  2:2328415/93‑1 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  2:4127868/93‑1 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  1:4448820/93‑1 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            >  2:3399402/1‑93 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  1:543237/93‑1 (MQ=255)
                                              atTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAt                                           >  2:711551/1‑93 (MQ=255)
                                               ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtg                                          <  2:1427813/93‑1 (MQ=255)
                                                tCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgt                                         <  2:1716723/93‑1 (MQ=255)
                                                tCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgt                                         >  2:406108/1‑93 (MQ=255)
                                                 cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgtg                                        >  1:2440069/1‑93 (MQ=255)
                                                  aTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTgg                                       >  2:1802561/1‑93 (MQ=255)
                                                   tttaGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga                                      <  2:3643692/90‑1 (MQ=255)
                                                    tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgac                                     <  2:3067125/93‑1 (MQ=255)
                                                    tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgac                                     >  1:940343/1‑93 (MQ=255)
                                                     gAGCATTCGTTTCACGCCAACCTGCCAGTCAGGAAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacg                                    <  1:4565536/93‑1 (MQ=255)
                                                       gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac                                  >  2:1665697/1‑93 (MQ=255)
                                                         aTTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAcgacga                                <  1:1402998/93‑1 (MQ=255)
                                                         aTTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAcgacga                                <  1:3219052/93‑1 (MQ=255)
                                                         aTTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAcgacga                                >  1:391747/1‑93 (MQ=255)
                                                          ttCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg                               >  1:1814774/1‑93 (MQ=255)
                                                            cGTGTCGCGCTAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACCACGAGCt                             <  1:3363259/93‑1 (MQ=255)
                                                              tttCACGCCAACCTGCCAGTCATGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                           >  1:842597/1‑93 (MQ=255)
                                                              tttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                           >  2:3351321/1‑93 (MQ=255)
                                                              tttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                           >  2:4100806/1‑93 (MQ=255)
                                                               ttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTggg                          >  2:4743989/1‑92 (MQ=255)
                                                                   cGCCACCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAg                      <  2:1832856/93‑1 (MQ=255)
                                                                   cGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAg                      <  1:477695/93‑1 (MQ=255)
                                                                    gCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAgg                     >  2:2440094/1‑93 (MQ=255)
                                                                      cAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAt                   <  1:3691081/93‑1 (MQ=255)
                                                                      cAACCCGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAt                   <  2:1149858/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  <  2:2532755/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  <  2:2353870/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  >  2:1260349/1‑93 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGAGCAGGATa                  >  2:627792/1‑93 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 >  1:862652/1‑93 (MQ=255)
                                                                         ccTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAgg                <  2:3518476/93‑1 (MQ=255)
                                                                            gCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtg             <  2:2572477/93‑1 (MQ=255)
                                                                              cAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgtt           >  2:4276448/1‑93 (MQ=255)
                                                                              cAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgtt           <  1:1834931/93‑1 (MQ=255)
                                                                                  cAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGttgttg       <  1:2122234/93‑1 (MQ=255)
                                                                                   aggcaaggcaAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTgg      >  2:2983664/1‑93 (MQ=255)
                                                                                   aggcaaggcaAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTgg      <  1:188829/93‑1 (MQ=255)
                                                                                    ggCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGt     <  2:1966324/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  >  NC_000913/2109073‑2109258
                                                                                             |                                                                                            
CCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGG                                                                                              >  SRR4302544.3869782/1‑94 (MQ=60)
 CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGC                                                                                             <  SRR4302544.3941793/94‑1 (MQ=60)
 CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGC                                                                                             >  SRR4302544.2602509/1‑94 (MQ=60)
 CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGC                                                                                             <  SRR4302544.1722468/94‑1 (MQ=60)
 CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGC                                                                                             >  SRR4302544.3011384/1‑94 (MQ=60)
  ATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCA                                                                                            >  SRR4302544.3643584/1‑94 (MQ=60)
    CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                          <  SRR4302544.3706249/94‑1 (MQ=60)
    CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                          <  SRR4302544.553864/94‑1 (MQ=60)
     ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                         >  SRR4302544.4565369/1‑94 (MQ=60)
     ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                         <  SRR4302544.4215768/94‑1 (MQ=60)
      CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                        <  SRR4302544.3662246/94‑1 (MQ=60)
      CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                        <  SRR4302544.2246003/94‑1 (MQ=60)
      CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                        <  SRR4302544.3300466/94‑1 (MQ=60)
        AACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCA                                                                                      <  SRR4302544.2979558/94‑1 (MQ=60)
         ACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGGGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAA                                                                                     <  SRR4302544.1019954/94‑1 (MQ=60)
          CAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                    <  SRR4302544.3121761/94‑1 (MQ=60)
           AAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAG                                                                                   >  SRR4302544.1646545/1‑94 (MQ=60)
           AAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAG                                                                                   >  SRR4302544.149220/1‑94 (MQ=60)
            AGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGT                                                                                  >  SRR4302544.4346059/1‑94 (MQ=60)
               TGCAAAAACTATTAAATTGTTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCAAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302544.1319644/94‑1 (MQ=60)
               TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302544.757180/94‑1 (MQ=60)
               TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302544.3015146/94‑1 (MQ=60)
               TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302544.1638599/94‑1 (MQ=60)
                GCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTG                                                                              <  SRR4302544.916563/94‑1 (MQ=60)
                GCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTG                                                                              >  SRR4302544.4742880/1‑94 (MQ=60)
                  AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCT                                                                            >  SRR4302544.1499111/1‑94 (MQ=60)
                   AAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTG                                                                           >  SRR4302544.3552280/1‑94 (MQ=60)
                    AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                          >  SRR4302544.2782711/1‑94 (MQ=60)
                    AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                          >  SRR4302544.2601818/1‑94 (MQ=60)
                     AACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAA                                                                         <  SRR4302544.142509/94‑1 (MQ=60)
                     AACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAA                                                                         >  SRR4302544.2636884/1‑94 (MQ=60)
                      ACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAA                                                                        >  SRR4302544.4059754/1‑94 (MQ=60)
                          TTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTT                                                                    <  SRR4302544.2698896/94‑1 (MQ=60)
                            AAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTC                                                                  >  SRR4302544.2568084/1‑94 (MQ=60)
                            AAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTC                                                                  >  SRR4302544.2803759/1‑94 (MQ=60)
                             AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                                 >  SRR4302544.929638/1‑94 (MQ=60)
                             AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACATGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                                 >  SRR4302544.3791198/1‑94 (MQ=60)
                             AATTGCTGAAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                                 <  SRR4302544.4586103/94‑1 (MQ=60)
                              agtggTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGTGCAGAGTTCTGCTGAAATTTTTCTG                                                                <  SRR4302544.602184/89‑1 (MQ=60)
                              ATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTG                                                                <  SRR4302544.2440660/94‑1 (MQ=60)
                              ATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTG                                                                <  SRR4302544.3213919/94‑1 (MQ=60)
                              ATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTG                                                                <  SRR4302544.602185/94‑1 (MQ=60)
                               TTGCTGTATTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                               <  SRR4302544.898864/94‑1 (MQ=60)
                               TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                               <  SRR4302544.522999/94‑1 (MQ=60)
                               TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                               >  SRR4302544.1837860/1‑94 (MQ=60)
                                 GCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAT                                                             <  SRR4302544.1889815/94‑1 (MQ=60)
                                  CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                            >  SRR4302544.555337/1‑94 (MQ=60)
                                  CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                            <  SRR4302544.128126/94‑1 (MQ=60)
                                   TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           >  SRR4302544.3809689/1‑94 (MQ=60)
                                   TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           <  SRR4302544.3003687/94‑1 (MQ=60)
                                    GTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAA                                                          >  SRR4302544.356591/1‑94 (MQ=60)
                                     TAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                         <  SRR4302544.4483944/94‑1 (MQ=60)
                                     TAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                         <  SRR4302544.3719655/94‑1 (MQ=60)
                                     TAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                         >  SRR4302544.3365129/1‑94 (MQ=60)
                                       GTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                       <  SRR4302544.3196831/94‑1 (MQ=60)
                                        TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      <  SRR4302544.3937598/94‑1 (MQ=60)
                                        TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      <  SRR4302544.3937728/94‑1 (MQ=60)
                                        TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      >  SRR4302544.4779632/1‑94 (MQ=60)
                                        TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGAAAAGTTCTGCTGACATTTTTCTGTATTAAGGCG                                                      >  SRR4302544.4779713/1‑94 (MQ=60)
                                         CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGA                                                     >  SRR4302544.1412921/1‑94 (MQ=60)
                                         CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGA                                                     >  SRR4302544.3108483/1‑94 (MQ=60)
                                         CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGA                                                     >  SRR4302544.2861596/1‑94 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    >  SRR4302544.4376450/1‑94 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    <  SRR4302544.2794213/94‑1 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    >  SRR4302544.29010/1‑94 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    <  SRR4302544.2794214/94‑1 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    >  SRR4302544.933953/1‑94 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    >  SRR4302544.2648621/1‑94 (MQ=60)
                                          GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGACAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                    >  SRR4302544.1930201/1‑94 (MQ=60)
                                           TAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGA                                                   >  SRR4302544.4309948/1‑94 (MQ=60)
                                              ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                                >  SRR4302544.1289206/1‑94 (MQ=60)
                                              ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                                >  SRR4302544.1406080/1‑94 (MQ=60)
                                               TAATTCATTGAGCATTCGTTTCACGCCAGCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTA                                               <  SRR4302544.832329/94‑1 (MQ=60)
                                               TAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTA                                               <  SRR4302544.580654/94‑1 (MQ=60)
                                                AATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAT                                              <  SRR4302544.4493642/94‑1 (MQ=60)
                                                AATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAT                                              <  SRR4302544.2296015/94‑1 (MQ=60)
                                                AATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAT                                              <  SRR4302544.548581/94‑1 (MQ=60)
                                                 ATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATG                                             <  SRR4302544.341809/94‑1 (MQ=60)
                                                   TCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTG                                           >  SRR4302544.2463335/1‑94 (MQ=60)
                                                    CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          <  SRR4302544.186782/94‑1 (MQ=60)
                                                    CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          >  SRR4302544.983022/1‑94 (MQ=60)
                                                    CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCCGAAATTTTTCTGTATTAAGGCGAGAGATATGTGG                                          >  SRR4302544.2453752/1‑94 (MQ=60)
                                                     ATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGA                                         >  SRR4302544.42708/1‑94 (MQ=60)
                                                      TTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAC                                        >  SRR4302544.949601/1‑94 (MQ=60)
                                                       TGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACG                                       <  SRR4302544.3092987/94‑1 (MQ=60)
                                                        GAGCATTCGTTTCACGCCAACCTGCCAGTCAGGAAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGA                                      <  SRR4302544.4611054/94‑1 (MQ=60)
                                                           CATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGA                                   <  SRR4302544.85030/94‑1 (MQ=60)
                                                           CATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGA                                   >  SRR4302544.395721/1‑94 (MQ=60)
                                                            ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  >  SRR4302544.4314334/1‑94 (MQ=60)
                                                            ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  <  SRR4302544.3249763/94‑1 (MQ=60)
                                                            ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  >  SRR4302544.1832488/1‑94 (MQ=60)
                                                            ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  <  SRR4302544.1416972/94‑1 (MQ=60)
                                                            ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  >  SRR4302544.2344477/1‑94 (MQ=60)
                                                              TCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCT                                <  SRR4302544.400820/94‑1 (MQ=60)
                                                               CGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTG                               >  SRR4302544.2781247/1‑94 (MQ=60)
                                                               CGTGTCGCGCTAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACCACGAGCTG                               <  SRR4302544.3395360/94‑1 (MQ=60)
                                                                GTTTCACGCCAACCTGCCAGTCATGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGG                              >  SRR4302544.850917/1‑94 (MQ=60)
                                                                GTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGG                              >  SRR4302544.3232217/1‑94 (MQ=60)
                                                                GTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGG                              >  SRR4302544.3232216/1‑94 (MQ=60)
                                                                 TTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGT                             <  SRR4302544.842258/94‑1 (MQ=60)
                                                                     ACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAG                         >  SRR4302544.3820018/1‑94 (MQ=60)
                                                                     ACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAG                         >  SRR4302544.2602915/1‑94 (MQ=60)
                                                                     ACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAG                         <  SRR4302544.2657262/94‑1 (MQ=60)
                                                                      CGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGG                        <  SRR4302544.482371/94‑1 (MQ=60)
                                                                        CCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAT                      <  SRR4302544.2845441/94‑1 (MQ=60)
                                                                         CAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATA                     <  SRR4302544.3727862/94‑1 (MQ=60)
                                                                          AACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAG                    >  SRR4302544.1710130/1‑94 (MQ=60)
                                                                          AACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAG                    >  SRR4302544.871187/1‑94 (MQ=60)
                                                                            CCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGC                  >  SRR4302544.859439/1‑94 (MQ=60)
                                                                             CTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCT                 >  SRR4302544.3589433/1‑94 (MQ=60)
                                                                                 CAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTG             <  SRR4302544.1852750/94‑1 (MQ=60)
                                                                                   GTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTT           <  SRR4302544.2795676/94‑1 (MQ=60)
                                                                                   GTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTT           <  SRR4302544.3011972/94‑1 (MQ=60)
                                                                                    TCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTG          <  SRR4302544.4627532/94‑1 (MQ=60)
                                                                                     CAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGG         >  SRR4302544.3944287/1‑94 (MQ=60)
                                                                                     CAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGG         <  SRR4302544.2142599/94‑1 (MQ=60)
                                                                                      AGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGT        <  SRR4302544.1220036/94‑1 (MQ=60)
                                                                                      AGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGT        <  SRR4302544.190711/94‑1 (MQ=60)
                                                                                         CAAGGCAATCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     <  SRR4302544.2760992/94‑1 (MQ=60)
                                                                                         CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     >  SRR4302544.2242516/1‑94 (MQ=60)
                                                                                         CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     >  SRR4302544.4517028/1‑94 (MQ=60)
                                                                                         CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     >  SRR4302544.3696/1‑94 (MQ=60)
                                                                                          AAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG    <  SRR4302544.3278997/94‑1 (MQ=60)
                                                                                           AGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGC   <  SRR4302544.2075752/94‑1 (MQ=60)
                                                                                           AGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGC   >  SRR4302544.2775331/1‑94 (MQ=60)
                                                                                           AGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGC   <  SRR4302544.4496374/94‑1 (MQ=60)
                                                                                           AGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGC   >  SRR4302544.1185775/1‑94 (MQ=60)
                                                                                            GGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  <  SRR4302544.2582012/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  >  NC_000913/2109073‑2109258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑