Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,654	T→C	A232A (GCA→GCG)	galF ←	putative a UDP‑gal transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,654	0	T	C	99.4%	505.2 / ‑4.8	157	A232A (GCA→GCG)	galF	putative a UDP‑gal transferase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (70/86); total (71/86)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 160 total aligned reads displayed.

GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT  >  NC_000913/2111564‑2111742

 tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCATTCAGCTGAATACGTCCCCACGCa                                                                                       >  2:2619502/1‑93 (MQ=255)

 tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                                                                                       >  2:3667027/1‑93 (MQ=255)

  cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                      <  2:1362133/93‑1 (MQ=255)

   gTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcc                                                                                     >  2:1733716/1‑93 (MQ=255)

    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                    >  2:3228195/1‑93 (MQ=255)

    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                    >  2:3228194/1‑93 (MQ=255)

     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                   <  2:800278/93‑1 (MQ=255)

     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                   >  1:4117399/1‑93 (MQ=255)

       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                 >  2:1714688/1‑93 (MQ=255)

       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                 <  1:3425725/93‑1 (MQ=255)

         gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg                                                                               >  1:1572611/1‑93 (MQ=255)

          tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                              <  2:2134063/93‑1 (MQ=255)

            gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCCGGCTGAGt                                                                            >  1:4304986/1‑93 (MQ=255)

            gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt                                                                            >  1:317142/1‑93 (MQ=255)

             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                           <  1:3421316/93‑1 (MQ=255)

             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                           <  1:1654458/93‑1 (MQ=255)

                  catcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTc                                                                      <  1:1314272/93‑1 (MQ=255)

                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:1775123/93‑1 (MQ=255)

                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  1:2249166/93‑1 (MQ=255)

                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:662641/93‑1 (MQ=255)

                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     <  2:1775124/93‑1 (MQ=255)

                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                     >  1:2980763/1‑93 (MQ=255)

                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                    >  1:1772204/1‑93 (MQ=255)

                      aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt                                                                  >  1:2702497/1‑93 (MQ=255)

                        cgttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTc                                                                <  2:3641006/91‑1 (MQ=255)

                         gttGCATCAACAGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTcc                                                               >  2:67758/2‑93 (MQ=255)

                          ttGCATCAACGGATTGTTTTGTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCg                                                              <  1:1990606/93‑1 (MQ=255)

                            gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                            <  1:963101/93‑1 (MQ=255)

                            gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                            >  2:2140314/1‑93 (MQ=255)

                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCa                                                          >  1:1105026/1‑93 (MQ=255)

                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGCTCCGGCCa                                                          >  1:1703364/1‑93 (MQ=255)

                                cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                        <  2:4267701/93‑1 (MQ=255)

                                cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                        <  1:2294609/93‑1 (MQ=255)

                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCGAGTTCCGGCCAAat                                                       >  1:678237/1‑93 (MQ=255)

                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       >  1:4337433/1‑93 (MQ=255)

                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       >  2:856422/1‑93 (MQ=255)

                                 aaCGGAATGGTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                       <  2:4641547/93‑1 (MQ=255)

                                  aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAata                                                      <  2:3474858/93‑1 (MQ=255)

                                   cGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCTGCCAAatat                                                     >  2:2370085/1‑93 (MQ=255)

                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  1:4748744/93‑1 (MQ=255)

                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  2:443063/93‑1 (MQ=255)

                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  2:4543333/93‑1 (MQ=255)

                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                    <  1:751805/93‑1 (MQ=255)

                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:2603388/1‑93 (MQ=255)

                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   <  2:2819845/93‑1 (MQ=255)

                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:1160370/1‑93 (MQ=255)

                                     gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   >  2:3832740/1‑93 (MQ=255)

                                     gATTGTTTTTTCGCCAGCTCGGCAAGAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                   <  1:270715/93‑1 (MQ=255)

                                         gTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAg                                               <  1:1232948/93‑1 (MQ=255)

                                           tttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAgca                                             >  1:969630/1‑91 (MQ=255)

                                            ttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaaa                                            <  2:2085410/93‑1 (MQ=255)

                                                 gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                       <  1:547344/93‑1 (MQ=255)

                                                 gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                       >  2:3756588/1‑93 (MQ=255)

                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                      >  1:1991396/1‑93 (MQ=255)

                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  2:2752770/93‑1 (MQ=255)

                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  2:4254074/93‑1 (MQ=255)

                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                     <  1:4627411/93‑1 (MQ=255)

                                                    aGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAg                                    >  2:559772/1‑93 (MQ=255)

                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                   <  1:1637594/93‑1 (MQ=255)

                                                      cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                  >  2:4649931/1‑93 (MQ=255)

                                                      cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                  <  1:1171127/93‑1 (MQ=255)

                                                       tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                 >  1:3733109/1‑93 (MQ=255)

                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                <  1:416653/93‑1 (MQ=255)

                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                >  2:3563064/1‑93 (MQ=255)

                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGAACATAGCGAc                                >  2:937088/1‑93 (MQ=255)

                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                               >  1:3383654/1‑93 (MQ=255)

                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                               <  1:4441046/93‑1 (MQ=255)

                                                          gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                              <  2:2658897/93‑1 (MQ=255)

                                                          gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                              >  2:2337489/1‑93 (MQ=255)

                                                               aGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                         >  2:2326215/1‑93 (MQ=255)

                                                               aGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                         >  1:3127388/1‑93 (MQ=255)

                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                        >  2:3469966/1‑93 (MQ=255)

                                                                  aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                      >  1:3055945/1‑93 (MQ=255)

                                                                  aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                      >  1:2743429/1‑93 (MQ=255)

                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                     >  1:1027610/1‑93 (MQ=255)

                                                                   tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                     <  2:1659566/93‑1 (MQ=255)

                                                                    cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                    <  2:2816583/93‑1 (MQ=255)

                                                                    cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                    >  2:3256989/1‑93 (MQ=255)

                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                  >  1:469431/1‑93 (MQ=255)

                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                  <  2:337688/93‑1 (MQ=255)

                                                                       tcagCTGAATACGTCCCCACGCACCAGGCTGATTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGt                 >  2:3639559/1‑93 (MQ=255)

                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                <  2:3771853/93‑1 (MQ=255)

                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                <  1:2643393/93‑1 (MQ=255)

                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt               >  1:3610248/1‑93 (MQ=255)

                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt               <  2:1863528/93‑1 (MQ=255)

                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg              <  2:3360636/93‑1 (MQ=255)

                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa             <  1:2563753/93‑1 (MQ=255)

                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa             <  1:1756613/93‑1 (MQ=255)

                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  2:1081978/1‑93 (MQ=255)

                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  1:52284/1‑93 (MQ=255)

                                                                            tGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAg            >  2:622777/1‑93 (MQ=255)

                                                                             gAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGt           <  1:3010565/93‑1 (MQ=255)

                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         <  1:3244335/93‑1 (MQ=255)

                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         >  2:4164044/1‑93 (MQ=255)

                                                                               aTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTcc         >  1:1668177/1‑93 (MQ=255)

                                                                                 aCGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAg       >  1:3889826/1‑93 (MQ=255)

                                                                                  cGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGc      <  1:1993254/93‑1 (MQ=255)

                                                                                    gccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGt    <  2:2534198/92‑1 (MQ=255)

                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc   <  2:1831252/93‑1 (MQ=255)

                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt  <  1:3143500/93‑1 (MQ=255)

GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT  >  NC_000913/2111564‑2111742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGG  >  NC_000913/2111562‑2111746

CAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACG                                                                                             >  SRR4302544.3235612/1‑94 (MQ=60)

CAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACG                                                                                             >  SRR4302544.3235613/1‑94 (MQ=60)

     GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                        <  SRR4302544.3416649/94‑1 (MQ=60)

     GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                        >  SRR4302544.4297446/1‑94 (MQ=60)

      TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                                                                                       >  SRR4302544.4158285/1‑94 (MQ=60)

        ACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGC                                                                                     >  SRR4302544.3116966/1‑94 (MQ=60)

         CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                                                                                    <  SRR4302544.3458477/94‑1 (MQ=60)

          TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTG                                                                                   >  SRR4302544.1588160/1‑94 (MQ=60)

             CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCCGGCTGAGT                                                                                >  SRR4302544.4348892/1‑94 (MQ=60)

             CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGT                                                                                >  SRR4302544.320440/1‑94 (MQ=60)

               CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                              >  SRR4302544.3827779/1‑94 (MQ=60)

               CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                              <  SRR4302544.1670775/94‑1 (MQ=60)

               CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                              <  SRR4302544.3454030/94‑1 (MQ=60)

                    CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC                                                                         <  SRR4302544.1327195/94‑1 (MQ=60)

                    CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC                                                                         >  SRR4302544.3009350/1‑94 (MQ=60)

                     ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                        <  SRR4302544.2270304/94‑1 (MQ=60)

                     ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                        >  SRR4302544.1789548/1‑94 (MQ=60)

                       CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT                                                                      >  SRR4302544.2728480/1‑94 (MQ=60)

                        AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT                                                                     >  SRR4302544.2713404/1‑94 (MQ=60)

                            TTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                 <  SRR4302544.2009930/94‑1 (MQ=60)

                            TTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                 <  SRR4302544.1123892/94‑1 (MQ=60)

                            TTGCATCAACGGATTGTTTTGTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                 <  SRR4302544.2009931/94‑1 (MQ=60)

                             TGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGC                                                                >  SRR4302544.1297239/1‑94 (MQ=60)

                             TGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGC                                                                <  SRR4302544.624149/94‑1 (MQ=60)

                              GCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCC                                                               <  SRR4302544.972559/94‑1 (MQ=60)

                               CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCA                                                              >  SRR4302544.1116034/1‑94 (MQ=60)

                               CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGCTCCGGCCA                                                              >  SRR4302544.1720169/1‑94 (MQ=60)

                                  CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCGAGTTCCGGCCAAAT                                                           >  SRR4302544.684960/1‑94 (MQ=60)

                                  CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAAT                                                           >  SRR4302544.4790572/1‑94 (MQ=60)

                                  CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAAT                                                           >  SRR4302544.4381574/1‑94 (MQ=60)

                                  CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAAT                                                           >  SRR4302544.2731255/1‑94 (MQ=60)

                                  CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAAT                                                           <  SRR4302544.2316239/94‑1 (MQ=60)

                                      GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                       <  SRR4302544.4795517/94‑1 (MQ=60)

                                      GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                       <  SRR4302544.759146/94‑1 (MQ=60)

                                      GGATTGTTTTTTCGACAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                       <  SRR4302544.1741177/94‑1 (MQ=60)

                                       GATTGTTTTTTCGCCAGCTCGGCAAGAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGG                                                      <  SRR4302544.273570/94‑1 (MQ=60)

                                          TGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAG                                                   <  SRR4302544.460216/94‑1 (MQ=60)

                                           GTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGA                                                  <  SRR4302544.1245144/94‑1 (MQ=60)

                                            TTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGCA                                                 >  SRR4302544.979137/1‑94 (MQ=60)

                                               TTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGC                                              <  SRR4302544.4126416/94‑1 (MQ=60)

                                                   GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                          >  SRR4302544.2010728/1‑94 (MQ=60)

                                                   GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                          <  SRR4302544.552715/94‑1 (MQ=60)

                                                    CCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATA                                         <  SRR4302544.1696865/94‑1 (MQ=60)

                                                     CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAG                                        <  SRR4302544.4673294/94‑1 (MQ=60)

                                                      AGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGC                                       <  SRR4302544.532548/94‑1 (MQ=60)

                                                       GCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCG                                      <  SRR4302544.1653721/94‑1 (MQ=60)

                                                        CTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGA                                     >  SRR4302544.3770225/1‑94 (MQ=60)

                                                        CTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGA                                     <  SRR4302544.1182814/94‑1 (MQ=60)

                                                          CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                   >  SRR4302544.3415981/1‑94 (MQ=60)

                                                          CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                   <  SRR4302544.420905/94‑1 (MQ=60)

                                                          CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                   <  SRR4302544.1656460/94‑1 (MQ=60)

                                                           GGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCT                                  <  SRR4302544.4485821/94‑1 (MQ=60)

                                                              AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGAACATAGCGACCTACG                               <  SRR4302544.3384692/94‑1 (MQ=60)

                                                                TAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGC                             >  SRR4302544.3157109/1‑94 (MQ=60)

                                                                   CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                          >  SRR4302544.3085121/1‑94 (MQ=60)

                                                                   CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                          >  SRR4302544.2769869/1‑94 (MQ=60)

                                                                   CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                          <  SRR4302544.439013/94‑1 (MQ=60)

                                                                    ATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATG                         >  SRR4302544.1037857/1‑94 (MQ=60)

                                                                       AGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATG                      >  SRR4302544.474023/1‑94 (MQ=60)

                                                                          CAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCT                   >  SRR4302544.3645509/1‑94 (MQ=60)

                                                                          CAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCT                   >  SRR4302544.2844351/1‑94 (MQ=60)

                                                                          CAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCT                   <  SRR4302544.2668739/94‑1 (MQ=60)

                                                                             CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG                <  SRR4302544.2588384/94‑1 (MQ=60)

                                                                             CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG                <  SRR4302544.1773805/94‑1 (MQ=60)

                                                                             CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG                >  SRR4302544.52814/1‑94 (MQ=60)

                                                                              TGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGT               >  SRR4302544.3068442/1‑94 (MQ=60)

                                                                               GAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTC              <  SRR4302544.3039422/94‑1 (MQ=60)

                                                                               GAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTC              <  SRR4302544.837767/94‑1 (MQ=60)

                                                                                AATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCC             <  SRR4302544.1491246/94‑1 (MQ=60)

                                                                                AATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCC             >  SRR4302544.1684608/1‑94 (MQ=60)

                                                                                 ATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCA            <  SRR4302544.3275395/94‑1 (MQ=60)

                                                                                  TACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAG           <  SRR4302544.3618486/94‑1 (MQ=60)

                                                                                  TACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAG           >  SRR4302544.3928461/1‑94 (MQ=60)

                                                                                    CGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCG         <  SRR4302544.2012606/94‑1 (MQ=60)

                                                                                    CGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCG         <  SRR4302544.2012607/94‑1 (MQ=60)

                                                                                    CGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCG         >  SRR4302544.2337453/1‑94 (MQ=60)

                                                                                    CGTCCCCACGCACCAGGCTGAGTACGTTACAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGGCCAGCG         <  SRR4302544.2012605/94‑1 (MQ=60)

                                                                                        CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG     <  SRR4302544.3173386/94‑1 (MQ=60)

                                                                                        CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG     <  SRR4302544.3173385/94‑1 (MQ=60)

                                                                                          CACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGGCCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG   <  SRR4302544.3828157/94‑1 (MQ=60)

                                                                                           ACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGG  <  SRR4302544.1689018/94‑1 (MQ=60)

CAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGG  >  NC_000913/2111562‑2111746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`