Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,654 T→C A232A (GCA→GCG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,6540TC100.0% 673.4 / NA 207A232A (GCA→GCG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/109);  total (98/109)

Only 100 of 215 total aligned reads displayed.

GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG  >  NC_000913/2111564‑2111743
                                                                                          |                                                                                         
ggcGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                                                                                         >  1:3469519/3‑93 (MQ=255)
gTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                                                                                         >  1:5526355/1‑93 (MQ=255)
gTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                                                                                         <  1:838367/93‑1 (MQ=255)
 tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                                                                                        >  1:5078299/1‑93 (MQ=255)
  cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                       >  2:1256474/1‑93 (MQ=255)
  cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                       <  1:6628066/93‑1 (MQ=255)
    tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                     >  1:2482899/1‑93 (MQ=255)
     aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                                                                                    >  1:5697557/1‑93 (MQ=255)
      aCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAgg                                                                                   >  2:4965115/1‑93 (MQ=255)
       cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                  >  2:5159398/1‑93 (MQ=255)
        tGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCt                                                                                 >  1:2550156/1‑93 (MQ=255)
         gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGGCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg                                                                                >  1:5826155/1‑93 (MQ=255)
          tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                               <  1:3730789/93‑1 (MQ=255)
             ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                            <  1:5144445/93‑1 (MQ=255)
              cGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAc                                                                           <  1:634765/93‑1 (MQ=255)
               ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCCGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg                                                                          >  2:3369218/1‑93 (MQ=255)
               ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg                                                                          >  2:6280884/1‑93 (MQ=255)
                gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                         >  2:579709/1‑93 (MQ=255)
                gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                         <  1:2894505/93‑1 (MQ=255)
                 tcatcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGtt                                                                        >  2:916370/1‑93 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                      <  2:5052203/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                      <  2:60789/93‑1 (MQ=255)
                   atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                      <  1:4972182/93‑1 (MQ=255)
                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                     >  2:5243437/1‑93 (MQ=255)
                    tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAAGTTccg                                                                     >  1:718346/1‑92 (MQ=255)
                         gttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTcc                                                                >  1:2386330/2‑93 (MQ=255)
                         gttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTcc                                                                <  2:2659842/92‑1 (MQ=255)
                           tGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCgg                                                              <  2:819311/93‑1 (MQ=255)
                           tGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCgg                                                              >  1:4501711/1‑93 (MQ=255)
                            gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                             <  2:1646810/93‑1 (MQ=255)
                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCa                                                           >  1:3015075/1‑93 (MQ=255)
                              aTCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCa                                                           >  2:3085750/1‑93 (MQ=255)
                               tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaa                                                          >  1:1193695/1‑93 (MQ=255)
                                 aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                        <  2:3763210/93‑1 (MQ=255)
                                  aCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAata                                                       >  2:5735754/1‑93 (MQ=255)
                                   cGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAatat                                                      <  1:3402319/93‑1 (MQ=255)
                                   cGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAatat                                                      <  2:1245139/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                     <  1:3436624/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                     <  1:1830962/93‑1 (MQ=255)
                                    ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                     <  2:1593701/93‑1 (MQ=255)
                                       ttGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGc                                                  >  2:4346976/1‑93 (MQ=255)
                                       ttGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGc                                                  <  1:3971499/93‑1 (MQ=255)
                                         gTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAg                                                >  1:212538/1‑93 (MQ=255)
                                         gTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAg                                                >  2:1720454/1‑93 (MQ=255)
                                          ttttttCGCTAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGa                                               >  2:3988203/1‑93 (MQ=255)
                                            ttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaaa                                             <  1:141601/93‑1 (MQ=255)
                                             tttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAg                                            <  2:6470118/93‑1 (MQ=255)
                                                cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcac                                         >  2:4177484/1‑93 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                       >  2:6613634/1‑93 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                       >  1:6286563/1‑93 (MQ=255)
                                                  ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                       <  1:6028007/93‑1 (MQ=255)
                                                   cAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATa                                      <  2:5284633/93‑1 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                    <  1:3860421/93‑1 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                    <  1:64692/93‑1 (MQ=255)
                                                     gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                    >  2:570756/1‑93 (MQ=255)
                                                       tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                  >  2:3537350/1‑93 (MQ=255)
                                                       tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                  >  2:2041050/1‑93 (MQ=255)
                                                       tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTGCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                  <  2:1236815/93‑1 (MQ=255)
                                                        cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                 <  2:1930157/93‑1 (MQ=255)
                                                         ggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                                >  2:2574579/1‑93 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             <  1:2070383/93‑1 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             >  1:973187/1‑93 (MQ=255)
                                                            aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                             >  1:621987/1‑93 (MQ=255)
                                                             aTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACg                            >  2:5606969/1‑93 (MQ=255)
                                                             aTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACg                            >  1:4367252/1‑93 (MQ=255)
                                                             aTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACg                            >  1:1584571/1‑93 (MQ=255)
                                                              tAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACgg                           <  1:5807965/93‑1 (MQ=255)
                                                               aGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                          >  2:1206872/1‑93 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                         >  2:2091812/1‑93 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                         <  1:3173251/93‑1 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGcc                         >  1:1776635/1‑93 (MQ=255)
                                                                gCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCAACCTACGGcc                         >  1:6172244/1‑93 (MQ=255)
                                                                    cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                     <  2:6525717/93‑1 (MQ=255)
                                                                     agtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgat                    <  1:448983/93‑1 (MQ=255)
                                                                     agtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgat                    <  1:4844074/93‑1 (MQ=255)
                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                   >  2:1014813/1‑93 (MQ=255)
                                                                      gtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatgatg                   >  1:495064/1‑93 (MQ=255)
                                                                        cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATATCGACCTACGGCCATGATGTc                 >  2:4729724/1‑93 (MQ=255)
                                                                         agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt                <  2:719375/93‑1 (MQ=255)
                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg               >  1:4760462/1‑93 (MQ=255)
                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg               <  1:1711601/93‑1 (MQ=255)
                                                                          gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg               >  1:4167013/1‑93 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa              <  2:1442608/93‑1 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa              <  2:4378348/93‑1 (MQ=255)
                                                                           cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa              <  1:377377/93‑1 (MQ=255)
                                                                                tACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa         <  2:5202828/93‑1 (MQ=255)
                                                                                tACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCa         <  2:5515963/93‑1 (MQ=255)
                                                                                  cGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGc       <  2:2484495/93‑1 (MQ=255)
                                                                                    tCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTGCAGCGt     >  1:2413744/1‑93 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    >  1:1957123/1‑93 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    <  1:262000/93‑1 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    <  1:2705431/93‑1 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    <  2:1873048/93‑1 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    <  1:1524613/93‑1 (MQ=255)
                                                                                     ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc    >  1:4586007/1‑93 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt   >  2:2618400/1‑93 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt   <  2:1754772/93‑1 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt   <  2:5668453/93‑1 (MQ=255)
                                                                                      cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt   >  1:2264391/1‑93 (MQ=255)
                                                                                       ccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTg  <  1:5307347/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTG  >  NC_000913/2111564‑2111743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGC  >  NC_000913/2111563‑2111747
                                                                                           |                                                                                             
AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCCTCAGTCAGCTGAATACGTCCACACGC                                                                                             >  SRR4302536.771472/1‑94 (MQ=60)
AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                                                                                             >  SRR4302536.5691135/1‑94 (MQ=60)
AGGCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                                                                                             >  SRR4302536.3572926/1‑94 (MQ=60)
 GTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCA                                                                                            <  SRR4302536.863017/94‑1 (MQ=60)
 GTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCA                                                                                            >  SRR4302536.5230280/1‑94 (MQ=60)
  TCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAC                                                                                           <  SRR4302536.5051281/94‑1 (MQ=60)
  TCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAC                                                                                           <  SRR4302536.1945142/94‑1 (MQ=60)
   CGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACC                                                                                          <  SRR4302536.6825210/94‑1 (MQ=60)
   CGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACC                                                                                          >  SRR4302536.4935181/1‑94 (MQ=60)
    GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                         >  SRR4302536.91870/1‑94 (MQ=60)
    GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                         >  SRR4302536.2557810/1‑94 (MQ=60)
    GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                                                                                         <  SRR4302536.3953482/94‑1 (MQ=60)
     TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                                                                                        >  SRR4302536.5866970/1‑94 (MQ=60)
      AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                                                                                       >  SRR4302536.5267189/1‑94 (MQ=60)
        CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                                                                                     >  SRR4302536.2626956/1‑94 (MQ=60)
        CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                                                                                     <  SRR4302536.2578673/94‑1 (MQ=60)
         TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGGCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTG                                                                                    >  SRR4302536.5999738/1‑94 (MQ=60)
          GTCGCCGGTCATCAGCGTTGCATCAGCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGA                                                                                   <  SRR4302536.5982179/94‑1 (MQ=60)
           TCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAG                                                                                  <  SRR4302536.3842308/94‑1 (MQ=60)
             GCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTA                                                                                >  SRR4302536.2607709/1‑94 (MQ=60)
             GCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTA                                                                                >  SRR4302536.1320484/1‑94 (MQ=60)
              CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                               <  SRR4302536.5297986/94‑1 (MQ=60)
              CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC                                                                               <  SRR4302536.2396781/94‑1 (MQ=60)
               CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG                                                                              <  SRR4302536.653530/94‑1 (MQ=60)
               CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG                                                                              <  SRR4302536.2069785/94‑1 (MQ=60)
                 GTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTT                                                                            <  SRR4302536.2979862/94‑1 (MQ=60)
                   CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC                                                                          <  SRR4302536.6185222/94‑1 (MQ=60)
                    ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                         <  SRR4302536.4159562/94‑1 (MQ=60)
                    ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                         <  SRR4302536.5121523/94‑1 (MQ=60)
                    ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA                                                                         <  SRR4302536.1885499/94‑1 (MQ=60)
                    ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAAGTTCCG                                                                         >  SRR4302536.739364/1‑94 (MQ=60)
                     TCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAG                                                                        <  SRR4302536.2937795/94‑1 (MQ=60)
                       AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT                                                                      >  SRR4302536.2506312/1‑94 (MQ=60)
                        GCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTC                                                                     >  SRR4302536.6428419/1‑94 (MQ=60)
                         CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC                                                                    <  SRR4302536.4084030/94‑1 (MQ=60)
                         CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC                                                                    >  SRR4302536.2458593/1‑94 (MQ=60)
                           TTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                  >  SRR4302536.4636813/1‑94 (MQ=60)
                           TTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGG                                                                  >  SRR4302536.2144098/1‑94 (MQ=60)
                           TTGCATCAACGGATTGCTTTTTCGCCAGCTCGGCAACAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTGCAGTTCCGG                                                                  >  SRR4302536.1671756/1‑94 (MQ=60)
                              CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCA                                                               >  SRR4302536.6377148/1‑94 (MQ=60)
                              CATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCA                                                               >  SRR4302536.3103703/1‑94 (MQ=60)
                               ATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAA                                                              >  SRR4302536.1229591/1‑94 (MQ=60)
                                 CAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGGCAAAT                                                            >  SRR4302536.4734459/1‑94 (MQ=60)
                                  AACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATA                                                           <  SRR4302536.4674029/94‑1 (MQ=60)
                                    CGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATC                                                         >  SRR4302536.3567149/1‑94 (MQ=60)
                                    CGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATC                                                         <  SRR4302536.3503512/94‑1 (MQ=60)
                                     GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                        <  SRR4302536.1886158/94‑1 (MQ=60)
                                     GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                        <  SRR4302536.3538948/94‑1 (MQ=60)
                                     GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                        <  SRR4302536.34987/94‑1 (MQ=60)
                                     GGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCG                                                        <  SRR4302536.6485418/94‑1 (MQ=60)
                                       ATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGC                                                      >  SRR4302536.4636296/1‑94 (MQ=60)
                                        TTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCA                                                     <  SRR4302536.4089382/94‑1 (MQ=60)
                                         TGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAG                                                    >  SRR4302536.218163/1‑94 (MQ=60)
                                         TGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAG                                                    <  SRR4302536.1577728/94‑1 (MQ=60)
                                             TTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAG                                                <  SRR4302536.145289/94‑1 (MQ=60)
                                             TTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAG                                                <  SRR4302536.1818391/94‑1 (MQ=60)
                                               TTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCA                                              <  SRR4302536.5652878/94‑1 (MQ=60)
                                               TTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCA                                              <  SRR4302536.5878244/94‑1 (MQ=60)
                                                  GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                           >  SRR4302536.6473905/1‑94 (MQ=60)
                                                  GCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAT                                           <  SRR4302536.3090789/94‑1 (MQ=60)
                                                   CCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATA                                          <  SRR4302536.6207740/94‑1 (MQ=60)
                                                   CCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATA                                          <  SRR4302536.3421783/94‑1 (MQ=60)
                                                    CAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAG                                         <  SRR4302536.5167174/94‑1 (MQ=60)
                                                     AGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGC                                        >  SRR4302536.3755854/1‑94 (MQ=60)
                                                      GCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCG                                       <  SRR4302536.3975298/94‑1 (MQ=60)
                                                      GCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCG                                       <  SRR4302536.66467/94‑1 (MQ=60)
                                                      GCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCG                                       <  SRR4302536.1568095/94‑1 (MQ=60)
                                                       CTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGA                                      <  SRR4302536.3851717/94‑1 (MQ=60)
                                                       CTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGA                                      <  SRR4302536.3267789/94‑1 (MQ=60)
                                                         CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                    <  SRR4302536.1974631/94‑1 (MQ=60)
                                                         CGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACC                                    >  SRR4302536.3202624/1‑94 (MQ=60)
                                                            CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAC                                 >  SRR4302536.1001905/1‑94 (MQ=60)
                                                            CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAC                                 >  SRR4302536.640459/1‑94 (MQ=60)
                                                            CAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGAGACCTAC                                 >  SRR4302536.4255507/1‑94 (MQ=60)
                                                             AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                                >  SRR4302536.4497740/1‑94 (MQ=60)
                                                             AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                                >  SRR4302536.1630954/1‑94 (MQ=60)
                                                             AATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACG                                <  SRR4302536.2132771/94‑1 (MQ=60)
                                                              ATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGG                               <  SRR4302536.6577324/94‑1 (MQ=60)
                                                              ATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGG                               <  SRR4302536.2485096/94‑1 (MQ=60)
                                                               TAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGC                              <  SRR4302536.5980950/94‑1 (MQ=60)
                                                                AGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCC                             >  SRR4302536.1830380/1‑94 (MQ=60)
                                                                AGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCAACCTACGGCC                             >  SRR4302536.6356092/1‑94 (MQ=60)
                                                                AGCATCAGACAGCTGAATAAGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGACAGCACATcgcgccctcacgcc                             >  SRR4302536.6679084/1‑80 (MQ=60)
                                                                 GCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCA                            <  SRR4302536.3266240/94‑1 (MQ=60)
                                                                  CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                           <  SRR4302536.5114267/94‑1 (MQ=60)
                                                                  CATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCAT                           >  SRR4302536.6524708/1‑94 (MQ=60)
                                                                   ATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATG                          <  SRR4302536.6279541/94‑1 (MQ=60)
                                                                    TCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGA                         >  SRR4302536.5827416/1‑94 (MQ=60)
                                                                     CAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGAT                        <  SRR4302536.6881944/94‑1 (MQ=60)
                                                                      AGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATG                       <  SRR4302536.460782/94‑1 (MQ=60)
                                                                      AGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATG                       >  SRR4302536.507850/1‑94 (MQ=60)
                                                                      AGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATG                       <  SRR4302536.4989854/94‑1 (MQ=60)
                                                                      AGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATG                       >  SRR4302536.2627418/1‑94 (MQ=60)
                                                                        TCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTC                     <  SRR4302536.4406800/94‑1 (MQ=60)
                                                                          AGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTG                   >  SRR4302536.4903821/1‑94 (MQ=60)
                                                                          AGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTG                   >  SRR4302536.4290815/1‑94 (MQ=60)
                                                                           GCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGA                  >  SRR4302536.5729592/1‑94 (MQ=60)
                                                                           GCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGA                  <  SRR4302536.1761123/94‑1 (MQ=60)
                                                                            CTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAG                 <  SRR4302536.387312/94‑1 (MQ=60)
                                                                            CTCAATACGTCCCCACGCACCAGGCTGACTACTTTCCAGTTCCGGCCAAATATCGGAAGAAAGAACATAGCGCCCTACGGCCATGATGTCTGAG                 <  SRR4302536.6591994/94‑1 (MQ=60)
                                                                               AATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCC              <  SRR4302536.1027307/94‑1 (MQ=60)
                                                                                    GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGGGACCTACGGACATGATGTCTGAGTCCAGCGT         >  SRR4302536.45263/1‑94 (MQ=60)
                                                                                    GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTGCAGCGT         >  SRR4302536.2486767/1‑94 (MQ=60)
                                                                                    GTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGT         >  SRR4302536.2730525/1‑94 (MQ=60)
                                                                                     TCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTC        >  SRR4302536.2015976/1‑94 (MQ=60)
                                                                                     TCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTC        >  SRR4302536.4723661/1‑94 (MQ=60)
                                                                                      CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT       >  SRR4302536.2332976/1‑94 (MQ=60)
                                                                                      CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT       <  SRR4302536.268995/94‑1 (MQ=60)
                                                                                      CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT       <  SRR4302536.2786154/94‑1 (MQ=60)
                                                                                      CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT       <  SRR4302536.1569483/94‑1 (MQ=60)
                                                                                      CCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCT       >  SRR4302536.5055639/1‑94 (MQ=60)
                                                                                       CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATCATGTCTGAGTACAGCGTCTG      <  SRR4302536.6765912/94‑1 (MQ=60)
                                                                                       CCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAACCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCCTCTG      <  SRR4302536.2198870/94‑1 (MQ=60)
                                                                                        CCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC     >  SRR4302536.3554170/1‑94 (MQ=60)
                                                                                        CCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC     <  SRR4302536.5347702/94‑1 (MQ=60)
                                                                                        CCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC     <  SRR4302536.5465738/94‑1 (MQ=60)
                                                                                         CACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG    <  SRR4302536.2494619/94‑1 (MQ=60)
                                                                                         CACGCACCACGATGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCG    <  SRR4302536.1660464/94‑1 (MQ=60)
                                                                                           CGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGC  >  SRR4302536.4186181/1‑94 (MQ=60)
                                                                                           |                                                                                             
AGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGCGGC  >  NC_000913/2111563‑2111747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑