Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,133,804 T→C H106R (CAC→CGC)  wzb ← putative protein‑tyrosine‑phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,8040TC99.4% 551.9 / ‑5.1 173H106R (CAC→CGC) wzbputative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (90/82);  total (91/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 177 total aligned reads displayed.

CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133714‑2133893
                                                                                          |                                                                                         
cGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGa                                                                                         >  1:2544075/1‑93 (MQ=255)
 gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAc                                                                                        >  2:4669843/1‑93 (MQ=255)
   gCAGACCGTTCAAGCAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCCGCGATTTCCAATTCCTTATCCCCGAGACCa                                                                                      >  2:370566/1‑93 (MQ=255)
    cAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaa                                                                                     <  1:1040396/93‑1 (MQ=255)
    cAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaa                                                                                     >  1:2099906/1‑93 (MQ=255)
       aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  >  2:4484932/1‑93 (MQ=255)
       aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  2:3422481/93‑1 (MQ=255)
        ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 >  2:95350/1‑93 (MQ=255)
        ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 <  2:2270456/93‑1 (MQ=255)
        ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 <  1:3552303/93‑1 (MQ=255)
         cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                <  1:2508670/93‑1 (MQ=255)
         cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                >  1:3627005/1‑93 (MQ=255)
          gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               <  1:3902146/93‑1 (MQ=255)
          gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               >  1:427496/1‑93 (MQ=255)
           ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCTGCGACCAAACAGCAt                                                                              >  1:1907155/1‑93 (MQ=255)
           ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  1:698436/93‑1 (MQ=255)
           ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              >  1:2248163/1‑93 (MQ=255)
           ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  2:2502315/93‑1 (MQ=255)
              aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCCAACAGGATCAc                                                                           >  1:1643246/1‑93 (MQ=255)
              aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  2:1351768/1‑93 (MQ=255)
              aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  1:3069734/1‑93 (MQ=255)
                gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  1:2371333/93‑1 (MQ=255)
                    tgtgTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGcc                                                                     <  1:513244/93‑1 (MQ=255)
                       gtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgcg                                                                  >  2:239324/1‑93 (MQ=255)
                        tACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCgcgc                                                                 >  1:109974/1‑93 (MQ=255)
                        tACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCgcgc                                                                 <  2:1301170/93‑1 (MQ=255)
                          cacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAt                                                               >  1:11886/1‑93 (MQ=255)
                           acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              >  2:4279974/1‑93 (MQ=255)
                             cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  1:3677614/93‑1 (MQ=255)
                              gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  1:1643168/93‑1 (MQ=255)
                              gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  2:2973242/93‑1 (MQ=255)
                                tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         >  1:4656603/1‑93 (MQ=255)
                                tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         >  1:872995/1‑93 (MQ=255)
                                 gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:1982653/93‑1 (MQ=255)
                                 gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        >  2:1543711/1‑93 (MQ=255)
                                 gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        >  1:1903189/1‑93 (MQ=255)
                                 gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:3320923/93‑1 (MQ=255)
                                 gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:2939330/93‑1 (MQ=255)
                                 gCAAACGTTGCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:1982654/93‑1 (MQ=255)
                                   aaaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGc                                                      <  1:4099221/93‑1 (MQ=255)
                                    aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  1:665303/1‑93 (MQ=255)
                                    aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACAAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  1:870227/1‑93 (MQ=255)
                                     aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    >  2:4348623/1‑93 (MQ=255)
                                       gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  2:1451185/1‑93 (MQ=255)
                                       gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  <  1:2347843/93‑1 (MQ=255)
                                       gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  1:3510095/1‑93 (MQ=255)
                                        tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 >  2:1877966/1‑93 (MQ=255)
                                        tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  1:4649910/93‑1 (MQ=255)
                                        tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  1:3467894/93‑1 (MQ=255)
                                         ttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtctc                                                <  2:1079061/93‑1 (MQ=255)
                                          tCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCg                                               >  2:3582416/1‑93 (MQ=255)
                                            ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:3893095/93‑1 (MQ=255)
                                            ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:4579798/93‑1 (MQ=255)
                                             cGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAt                                            >  1:1391128/1‑93 (MQ=255)
                                             cGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAt                                            >  1:1035994/1‑93 (MQ=255)
                                              ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           <  2:4273777/93‑1 (MQ=255)
                                              ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           <  1:4185001/93‑1 (MQ=255)
                                               gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          <  2:1903928/93‑1 (MQ=255)
                                               gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          >  1:1451899/1‑93 (MQ=255)
                                                 ttttTCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        <  1:2329160/93‑1 (MQ=255)
                                                 ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:4652417/1‑93 (MQ=255)
                                                 ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:3299644/1‑93 (MQ=255)
                                                    tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  1:1698569/93‑1 (MQ=255)
                                                       gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  >  1:620776/1‑93 (MQ=255)
                                                        aTACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 <  1:1798622/93‑1 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  1:676987/1‑93 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  2:615870/1‑93 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:3411612/93‑1 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  1:602166/1‑93 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  2:1389833/1‑93 (MQ=255)
                                                         tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAAAGCATCAGTTTGCCGGGCATCTCCGGTGCCATCTCGCATAAGCGTTAGata                                >  1:853391/1‑93 (MQ=255)
                                                          aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               >  1:541603/1‑93 (MQ=255)
                                                          aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               <  1:3208945/93‑1 (MQ=255)
                                                           cGGATTGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  1:793282/93‑1 (MQ=255)
                                                            ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGCTATgg                             >  1:618374/1‑93 (MQ=255)
                                                            ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  2:2552195/93‑1 (MQ=255)
                                                            ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  1:90080/93‑1 (MQ=255)
                                                            ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  1:2519594/93‑1 (MQ=255)
                                                             gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            <  1:3820822/93‑1 (MQ=255)
                                                               tCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                          <  1:1247687/93‑1 (MQ=255)
                                                               tCGGGGATTTCACATTCGTTATCCCAGCAACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                          <  1:4231579/93‑1 (MQ=255)
                                                                cGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCt                         <  1:2897045/93‑1 (MQ=255)
                                                                  gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       >  2:3933167/1‑93 (MQ=255)
                                                                   ggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtttt                      >  1:1728594/1‑93 (MQ=255)
                                                                      tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  1:3129955/1‑93 (MQ=255)
                                                                      tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  2:1745448/1‑93 (MQ=255)
                                                                       ttCACATTCGTTATCCCAGCGACCAAACAGCATCACTATGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  <  1:3229396/93‑1 (MQ=255)
                                                                        tCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATg                 >  1:1278004/1‑93 (MQ=255)
                                                                         cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                >  2:3037349/1‑93 (MQ=255)
                                                                           caTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTc              >  2:2526638/1‑93 (MQ=255)
                                                                               cGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          <  1:2942754/93‑1 (MQ=255)
                                                                                gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         <  2:264722/93‑1 (MQ=255)
                                                                                gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         <  1:3382456/93‑1 (MQ=255)
                                                                                   aTCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAg      >  1:2868349/1‑93 (MQ=255)
                                                                                   aTCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAg      <  1:1987033/93‑1 (MQ=255)
                                                                                     cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    >  2:2042878/1‑93 (MQ=255)
                                                                                      ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   <  1:2792658/93‑1 (MQ=255)
                                                                                      ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   >  2:4634595/1‑93 (MQ=255)
                                                                                       cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  >  1:4495138/1‑93 (MQ=255)
                                                                                       cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  >  2:1735430/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133714‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA  >  NC_000913/2133711‑2133895
                                                                                             |                                                                                           
TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGC                                                                                             >  SRR4302544.4444613/1‑94 (MQ=60)
TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGC                                                                                             >  SRR4302544.2520406/1‑94 (MQ=60)
 GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCG                                                                                            >  SRR4302544.1450868/1‑94 (MQ=60)
  GCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGA                                                                                           >  SRR4302544.2568499/1‑94 (MQ=60)
     GGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCA                                                                                        <  SRR4302544.953250/94‑1 (MQ=60)
      GCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAA                                                                                       >  SRR4302544.2120113/1‑94 (MQ=60)
       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                      <  SRR4302544.1050723/94‑1 (MQ=60)
       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                      >  SRR4302544.4566556/1‑94 (MQ=60)
       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                      <  SRR4302544.1418970/94‑1 (MQ=60)
       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAACAAA                                                                                      >  SRR4302544.2239856/1‑94 (MQ=60)
           CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                  <  SRR4302544.3586327/94‑1 (MQ=60)
           CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                  >  SRR4302544.3663136/1‑94 (MQ=60)
            CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                 <  SRR4302544.2532731/94‑1 (MQ=60)
            CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                 >  SRR4302544.3783408/1‑94 (MQ=60)
            CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                 >  SRR4302544.431841/1‑94 (MQ=60)
             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTTACATTCGTTATCCCAGCGACCAAACAACAT                                                                                >  SRR4302544.1227884/1‑94 (MQ=60)
             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCTGCGACCAAACAGCAT                                                                                >  SRR4302544.1925596/1‑94 (MQ=60)
             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                <  SRR4302544.3940907/94‑1 (MQ=60)
             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                >  SRR4302544.2269294/1‑94 (MQ=60)
              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                               <  SRR4302544.705393/94‑1 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCCAACAGGATCAC                                                                             >  SRR4302544.1659446/1‑94 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                             >  SRR4302544.1659447/1‑94 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                             >  SRR4302544.653450/1‑94 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                             >  SRR4302544.2561535/1‑94 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                             >  SRR4302544.3098987/1‑94 (MQ=60)
                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                             >  SRR4302544.562010/1‑94 (MQ=60)
                 AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGCTATCCCAGCGACCAAACAGCATCACT                                                                            >  SRR4302544.3026778/1‑94 (MQ=60)
                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                          <  SRR4302544.1735465/94‑1 (MQ=60)
                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                          <  SRR4302544.2393774/94‑1 (MQ=60)
                       TGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCG                                                                      <  SRR4302544.518311/94‑1 (MQ=60)
                          GTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCCAACAGCATCACTTTGCCGCGC                                                                   >  SRR4302544.812789/1‑94 (MQ=60)
                          GTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGC                                                                   <  SRR4302544.1398000/94‑1 (MQ=60)
                          GTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGC                                                                   >  SRR4302544.110981/1‑94 (MQ=60)
                           TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCA                                                                  >  SRR4302544.4416314/1‑94 (MQ=60)
                            ACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAT                                                                 <  SRR4302544.420428/94‑1 (MQ=60)
                            ACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAT                                                                 >  SRR4302544.11991/1‑94 (MQ=60)
                             CACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATC                                                                >  SRR4302544.2362751/1‑94 (MQ=60)
                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                             <  SRR4302544.3714295/94‑1 (MQ=60)
                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                            >  SRR4302544.3160404/1‑94 (MQ=60)
                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                            <  SRR4302544.1659368/94‑1 (MQ=60)
                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                            <  SRR4302544.3594539/94‑1 (MQ=60)
                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                            >  SRR4302544.50715/1‑94 (MQ=60)
                                  CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                           >  SRR4302544.4702675/1‑94 (MQ=60)
                                  CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                           >  SRR4302544.1762618/1‑94 (MQ=60)
                                  CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                           >  SRR4302544.881650/1‑94 (MQ=60)
                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                          <  SRR4302544.1860423/94‑1 (MQ=60)
                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                          >  SRR4302544.1921585/1‑94 (MQ=60)
                                     CAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGC                                                        >  SRR4302544.876387/1‑94 (MQ=60)
                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                       <  SRR4302544.3315593/94‑1 (MQ=60)
                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                       >  SRR4302544.671874/1‑94 (MQ=60)
                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                       <  SRR4302544.4140014/94‑1 (MQ=60)
                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                       >  SRR4302544.3551650/1‑94 (MQ=60)
                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACAAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                       >  SRR4302544.878850/1‑94 (MQ=60)
                                       AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                      <  SRR4302544.625063/94‑1 (MQ=60)
                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGTCAT                                                     >  SRR4302544.2915701/1‑94 (MQ=60)
                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                     >  SRR4302544.2511896/1‑94 (MQ=60)
                                         CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                    >  SRR4302544.3543584/1‑94 (MQ=60)
                                          GTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                   <  SRR4302544.2370085/94‑1 (MQ=60)
                                           TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                  <  SRR4302544.3500973/94‑1 (MQ=60)
                                           TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                  <  SRR4302544.3500972/94‑1 (MQ=60)
                                           TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                  <  SRR4302544.4695935/94‑1 (MQ=60)
                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                              <  SRR4302544.3931763/94‑1 (MQ=60)
                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                              <  SRR4302544.4625413/94‑1 (MQ=60)
                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                              >  SRR4302544.1046295/1‑94 (MQ=60)
                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                              >  SRR4302544.1405014/1‑94 (MQ=60)
                                                CGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATA                                             <  SRR4302544.2384295/94‑1 (MQ=60)
                                                CGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATA                                             >  SRR4302544.3946204/1‑94 (MQ=60)
                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                            <  SRR4302544.3940247/94‑1 (MQ=60)
                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                            <  SRR4302544.4226442/94‑1 (MQ=60)
                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                            >  SRR4302544.1466326/1‑94 (MQ=60)
                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                          >  SRR4302544.3115051/1‑94 (MQ=60)
                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                          >  SRR4302544.3331070/1‑94 (MQ=60)
                                                    TTTTTCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCG                                         <  SRR4302544.2351244/94‑1 (MQ=60)
                                                    TTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCG                                         <  SRR4302544.1534234/94‑1 (MQ=60)
                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCTCGCATCCCCGGTGCCATCTCGCATAAGCGTTC                                      <  SRR4302544.1715331/94‑1 (MQ=60)
                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                      <  SRR4302544.1715332/94‑1 (MQ=60)
                                                         CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                    >  SRR4302544.626865/1‑94 (MQ=60)
                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                   >  SRR4302544.1968956/1‑94 (MQ=60)
                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                  <  SRR4302544.1816206/94‑1 (MQ=60)
                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                  >  SRR4302544.608069/1‑94 (MQ=60)
                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                  >  SRR4302544.683694/1‑94 (MQ=60)
                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                  >  SRR4302544.683693/1‑94 (MQ=60)
                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAAAGCATCAGTTTGCCGGGCATCTCCGGTGCCATCTCGCATAAGCGTTAGATA                                  >  SRR4302544.861829/1‑94 (MQ=60)
                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                 <  SRR4302544.530493/94‑1 (MQ=60)
                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                 >  SRR4302544.546935/1‑94 (MQ=60)
                                                             GCGGATCGGGGATTTCACATTCGTTATCCCAGCGCCCAAACAGCATCGCTTTGCCGCGCCTCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                <  SRR4302544.2671137/94‑1 (MQ=60)
                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                <  SRR4302544.3390040/94‑1 (MQ=60)
                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                <  SRR4302544.3665591/94‑1 (MQ=60)
                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                <  SRR4302544.3239547/94‑1 (MQ=60)
                                                              CGGATTGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                               <  SRR4302544.800963/94‑1 (MQ=60)
                                                              CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGCTATGG                               >  SRR4302544.624446/1‑94 (MQ=60)
                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                              <  SRR4302544.2543799/94‑1 (MQ=60)
                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                              <  SRR4302544.90927/94‑1 (MQ=60)
                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                             <  SRR4302544.3858697/94‑1 (MQ=60)
                                                                 ATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGC                            >  SRR4302544.1136118/1‑94 (MQ=60)
                                                                  TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                           <  SRR4302544.1259975/94‑1 (MQ=60)
                                                                  TCGGGGATTTCACATTCGTTATCCCAGCAACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                           <  SRR4302544.4274696/94‑1 (MQ=60)
                                                                   CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                          <  SRR4302544.2924950/94‑1 (MQ=60)
                                                                    GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                         >  SRR4302544.1088581/1‑94 (MQ=60)
                                                                    GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                         <  SRR4302544.715040/94‑1 (MQ=60)
                                                                     GGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTT                        >  SRR4302544.1745544/1‑94 (MQ=60)
                                                                        ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                     >  SRR4302544.3159693/1‑94 (MQ=60)
                                                                         TTTCACATTCGTTATCCCAGCGAACAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                    <  SRR4302544.2017734/94‑1 (MQ=60)
                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                   >  SRR4302544.1290588/1‑94 (MQ=60)
                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                   >  SRR4302544.4281397/1‑94 (MQ=60)
                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                   <  SRR4302544.2865106/94‑1 (MQ=60)
                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTATGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                   <  SRR4302544.3260292/94‑1 (MQ=60)
                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT           >  SRR4302544.2813252/1‑94 (MQ=60)
                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT           <  SRR4302544.2971018/94‑1 (MQ=60)
                                                                                   GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC          <  SRR4302544.3414757/94‑1 (MQ=60)
                                                                                     TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG        >  SRR4302544.2896037/1‑94 (MQ=60)
                                                                                      ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG       <  SRR4302544.2006326/94‑1 (MQ=60)
                                                                                        CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC     >  SRR4302544.4700870/1‑94 (MQ=60)
                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG    >  SRR4302544.4540255/1‑94 (MQ=60)
                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG    <  SRR4302544.2819547/94‑1 (MQ=60)
                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG    <  SRR4302544.906419/94‑1 (MQ=60)
                                                                                          CAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGT   >  SRR4302544.4720132/1‑94 (MQ=60)
                                                                                           AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA  >  SRR4302544.2094314/1‑94 (MQ=60)
                                                                                             |                                                                                           
TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA  >  NC_000913/2133711‑2133895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑