Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,133,804 T→C H106R (CAC→CGC)  wzb ← putative protein‑tyrosine‑phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,8040TC100.0% 748.1 / NA 231H106R (CAC→CGC) wzbputative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/118);  total (113/118)

Only 100 of 235 total aligned reads displayed.

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893
                                                                                         |                                                                                         
gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAc                                                                                        >  2:1087803/1‑93 (MQ=255)
  gCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCCGGGACCa                                                                                      >  1:1514073/1‑93 (MQ=255)
  gCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCa                                                                                      <  2:3883115/93‑1 (MQ=255)
  gCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCa                                                                                      >  2:2760070/1‑93 (MQ=255)
   cAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaa                                                                                     >  2:6359399/1‑93 (MQ=255)
    aGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaaa                                                                                    >  1:2223410/1‑93 (MQ=255)
     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  2:1415402/1‑93 (MQ=255)
     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  2:3323894/1‑93 (MQ=255)
      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  2:6149547/93‑1 (MQ=255)
      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  2:3059392/93‑1 (MQ=255)
       ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 <  2:3816882/93‑1 (MQ=255)
       ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 <  1:262956/93‑1 (MQ=255)
         gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               >  2:517422/1‑93 (MQ=255)
         gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               >  2:3251795/1‑93 (MQ=255)
           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             <  1:5939179/93‑1 (MQ=255)
             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           <  1:3976497/93‑1 (MQ=255)
             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  1:3028632/1‑93 (MQ=255)
              aGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACt                                                                          <  1:6122542/93‑1 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         >  1:4243882/1‑93 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         >  1:6244138/1‑93 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  1:2037620/93‑1 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         >  2:44130/1‑93 (MQ=255)
                tAATGTGTACGCCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        <  2:361053/93‑1 (MQ=255)
                  aTGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGc                                                                      >  1:5378454/1‑93 (MQ=255)
                      gtACACCGCTGCAAGCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgcg                                                                  >  2:5222981/1‑93 (MQ=255)
                      gtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgcg                                                                  >  1:6150381/1‑93 (MQ=255)
                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAGCAAACAGCATCACTTTGCCGCGCa                                                                >  1:1436517/1‑93 (MQ=255)
                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                >  1:6494014/1‑93 (MQ=255)
                         cacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAt                                                               >  1:2561490/1‑93 (MQ=255)
                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  1:2408289/93‑1 (MQ=255)
                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             <  1:1770754/93‑1 (MQ=255)
                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  1:477848/93‑1 (MQ=255)
                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  1:3672242/93‑1 (MQ=255)
                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          >  1:904497/1‑93 (MQ=255)
                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          >  1:1658184/1‑93 (MQ=255)
                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         >  1:6292072/1‑93 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        >  2:1500284/1‑93 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  1:4672/93‑1 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:5431362/93‑1 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  2:2271470/1‑93 (MQ=255)
                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   <  1:5453725/93‑1 (MQ=255)
                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   <  1:4832407/93‑1 (MQ=255)
                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  <  2:4611668/93‑1 (MQ=255)
                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  <  2:1351770/93‑1 (MQ=255)
                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  2:6105252/1‑93 (MQ=255)
                                      ctttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  <  2:4247916/92‑1 (MQ=255)
                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  2:2997087/93‑1 (MQ=255)
                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 >  2:6583651/1‑93 (MQ=255)
                                          cccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGc                                              >  1:5958895/1‑93 (MQ=255)
                                            cGGCTTTTGCGGTTCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAt                                            <  2:569013/93‑1 (MQ=255)
                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          <  2:1864168/93‑1 (MQ=255)
                                               cTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAg                                         <  1:4279898/93‑1 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:397973/1‑93 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:1223678/1‑93 (MQ=255)
                                                 tttGTGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                       <  2:5259107/93‑1 (MQ=255)
                                                  ttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGt                                      <  2:535631/93‑1 (MQ=255)
                                                  ttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGt                                      <  1:2724327/93‑1 (MQ=255)
                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  2:5633607/93‑1 (MQ=255)
                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  2:655818/93‑1 (MQ=255)
                                                     cGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCg                                   <  2:2243496/93‑1 (MQ=255)
                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  <  1:6389169/93‑1 (MQ=255)
                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  >  2:23912/1‑93 (MQ=255)
                                                       aTACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 >  2:345571/1‑93 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  1:4924919/1‑93 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  2:6429994/1‑93 (MQ=255)
                                                        tACGGATCGGGGATCTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:2785755/93‑1 (MQ=255)
                                                         aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               <  2:1038144/93‑1 (MQ=255)
                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  2:1965373/93‑1 (MQ=255)
                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  1:353989/1‑93 (MQ=255)
                                                               cGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCt                         <  2:1210842/93‑1 (MQ=255)
                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  1:747278/1‑93 (MQ=255)
                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  2:3358851/1‑93 (MQ=255)
                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  2:1369661/1‑93 (MQ=255)
                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     <  2:5864456/93‑1 (MQ=255)
                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     >  1:5092867/1‑93 (MQ=255)
                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   <  2:3021670/93‑1 (MQ=255)
                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  1:1012490/1‑93 (MQ=255)
                                                                       tCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATg                 <  2:2687099/93‑1 (MQ=255)
                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgt                <  2:6319142/93‑2 (MQ=255)
                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               >  1:571291/1‑93 (MQ=255)
                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               <  2:2611400/93‑1 (MQ=255)
                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               >  1:3157647/1‑93 (MQ=255)
                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               <  1:5232120/93‑1 (MQ=255)
                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  1:4727442/1‑93 (MQ=255)
                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  1:4448309/1‑93 (MQ=255)
                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  2:5088135/1‑93 (MQ=255)
                                                                             tCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaa           <  2:701205/93‑1 (MQ=255)
                                                                              cGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          <  1:490567/93‑1 (MQ=255)
                                                                               gTTATCCCCGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         >  1:6544020/1‑93 (MQ=255)
                                                                               gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         <  1:6092074/93‑1 (MQ=255)
                                                                               gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         >  1:6710032/1‑93 (MQ=255)
                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCTTGGTCAAAATCa       >  2:4068814/1‑93 (MQ=255)
                                                                                  aTCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAg      >  2:763364/1‑93 (MQ=255)
                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  2:4832225/93‑1 (MQ=255)
                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  2:6680351/93‑1 (MQ=255)
                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  2:4104898/93‑1 (MQ=255)
                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   >  1:2904665/1‑93 (MQ=255)
                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   <  1:3063186/93‑1 (MQ=255)
                                                                                      cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  >  1:689927/1‑93 (MQ=255)
                                                                                      cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  >  2:154963/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897
                                                                                            |                                                                                             
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCG                                                                                              <  SRR4302536.6202385/94‑1 (MQ=60)
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCG                                                                                              >  SRR4302536.2649186/1‑94 (MQ=60)
  CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAC                                                                                            >  SRR4302536.4791861/1‑94 (MQ=60)
   TGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302536.4868958/1‑94 (MQ=60)
    GGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCCGGGACCA                                                                                          >  SRR4302536.1558658/1‑94 (MQ=60)
    GGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCA                                                                                          >  SRR4302536.6479871/1‑94 (MQ=60)
      CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        <  SRR4302536.2827363/94‑1 (MQ=60)
      CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        >  SRR4302536.2290658/1‑94 (MQ=60)
      CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        <  SRR4302536.519917/94‑1 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302536.5499013/1‑94 (MQ=60)
         ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302536.5008706/94‑1 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302536.269973/94‑1 (MQ=60)
            GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  >  SRR4302536.5734289/1‑94 (MQ=60)
              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                <  SRR4302536.6116112/94‑1 (MQ=60)
               CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               >  SRR4302536.3117626/1‑94 (MQ=60)
                AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACT                                                                              <  SRR4302536.4094535/94‑1 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             >  SRR4302536.6430153/1‑94 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             >  SRR4302536.4370269/1‑94 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             <  SRR4302536.6305010/94‑1 (MQ=60)
                  GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            <  SRR4302536.6816059/94‑1 (MQ=60)
                  GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            <  SRR4302536.2098982/94‑1 (MQ=60)
                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302536.6798494/94‑1 (MQ=60)
                    AATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGC                                                                          >  SRR4302536.5538927/1‑94 (MQ=60)
                    AATGCGTACACCGCTGCAAACGTGTCCCGGCTTTTGCGATACGGATCCGGGATTTCACCTTCGGTATCTCAGCGACCAAACAGCATCAGTTTGC                                                                          >  SRR4302536.497318/1‑94 (MQ=60)
                     ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAACAAACAGCATCACTTTGCC                                                                         >  SRR4302536.1368828/1‑94 (MQ=60)
                        TGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCG                                                                      >  SRR4302536.6333624/1‑94 (MQ=60)
                          TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAGCAAACAGCATCACTTTGCCGCGCA                                                                    >  SRR4302536.1479139/1‑94 (MQ=60)
                          TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCA                                                                    >  SRR4302536.6687629/1‑94 (MQ=60)
                           ACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAT                                                                   >  SRR4302536.2638575/1‑94 (MQ=60)
                            CACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATACCAGCGACCAAACAGCATCACTTTGCCGCGCATC                                                                  >  SRR4302536.2561215/1‑94 (MQ=60)
                             ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302536.1938271/94‑1 (MQ=60)
                             ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302536.2481141/94‑1 (MQ=60)
                              CCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTC                                                                <  SRR4302536.1824333/94‑1 (MQ=60)
                               CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302536.490293/94‑1 (MQ=60)
                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              >  SRR4302536.3172992/1‑94 (MQ=60)
                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302536.3782037/94‑1 (MQ=60)
                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              >  SRR4302536.931154/1‑94 (MQ=60)
                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              >  SRR4302536.1706331/1‑94 (MQ=60)
                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             >  SRR4302536.6479547/1‑94 (MQ=60)
                                  TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTTCGGT                                                            >  SRR4302536.5826506/1‑94 (MQ=60)
                                   GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           <  SRR4302536.4785/94‑1 (MQ=60)
                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302536.4119200/1‑94 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        <  SRR4302536.1501997/94‑1 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        <  SRR4302536.4927166/94‑1 (MQ=60)
                                        CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      <  SRR4302536.5616831/94‑1 (MQ=60)
                                        CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      <  SRR4302536.4977819/94‑1 (MQ=60)
                                         GTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                     <  SRR4302536.1761997/94‑1 (MQ=60)
                                          TTTCCCGGCTTTTGAGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    <  SRR4302536.3054803/94‑1 (MQ=60)
                                           TTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCG                                                   <  SRR4302536.5582555/94‑1 (MQ=60)
                                            TCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGC                                                  >  SRR4302536.6136463/1‑94 (MQ=60)
                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCC                                                 <  SRR4302536.3957682/94‑1 (MQ=60)
                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCA                                                 >  SRR4302536.2419576/1‑94 (MQ=60)
                                             ACCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGCTATGCCAGCGACCGAACAGCATCCCTTTGCCCCGCATCTCCCGCACCATCTCGCA                                                 >  SRR4302536.3064843/1‑94 (MQ=53)
                                              CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302536.6357178/94‑1 (MQ=60)
                                              CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302536.4918693/94‑1 (MQ=60)
                                               CGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATA                                               <  SRR4302536.6327949/94‑1 (MQ=60)
                                                GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              >  SRR4302536.2688112/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302536.1260453/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302536.4407565/94‑1 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302536.5288464/94‑1 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302536.6272638/94‑1 (MQ=60)
                                                   TTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCG                                           <  SRR4302536.90043/94‑1 (MQ=60)
                                                     TTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTT                                         <  SRR4302536.2805566/94‑1 (MQ=60)
                                                     TTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTT                                         <  SRR4302536.3226246/94‑1 (MQ=60)
                                                      TGCGATACGGATCGGGGATTTCACTTTCGTTCTCCCAGCGACCAAACAGCAACACTTTGCCGCGCATATCCAGAGACATCTCGCATAAGCGTTA                                        <  SRR4302536.6932232/94‑1 (MQ=60)
                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302536.6680543/1‑94 (MQ=60)
                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302536.624834/1‑94 (MQ=60)
                                                         GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     <  SRR4302536.6579798/94‑1 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302536.1904148/94‑1 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    >  SRR4302536.5072907/1‑94 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302536.390688/94‑1 (MQ=60)
                                                            ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                  <  SRR4302536.6043522/94‑1 (MQ=60)
                                                             CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 >  SRR4302536.799876/1‑94 (MQ=60)
                                                              GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302536.480404/94‑1 (MQ=60)
                                                               GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               >  SRR4302536.5067034/1‑94 (MQ=60)
                                                               GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               <  SRR4302536.1621752/94‑1 (MQ=60)
                                                               GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               >  SRR4302536.363358/1‑94 (MQ=60)
                                                                 TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             >  SRR4302536.6489259/1‑94 (MQ=60)
                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302536.2787449/1‑94 (MQ=60)
                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302536.710677/1‑94 (MQ=60)
                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302536.769230/1‑94 (MQ=60)
                                                                    GGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTT                          <  SRR4302536.1501956/94‑1 (MQ=60)
                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         <  SRR4302536.6713474/94‑1 (MQ=60)
                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         >  SRR4302536.5245168/1‑94 (MQ=60)
                                                                       ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302536.1042381/1‑94 (MQ=60)
                                                                       ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302536.2549362/1‑94 (MQ=60)
                                                                        TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302536.3138354/94‑1 (MQ=60)
                                                                         TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                     >  SRR4302536.6291558/1‑94 (MQ=60)
                                                                          TCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGG                    <  SRR4302536.2814614/94‑1 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302536.5270162/1‑94 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302536.585819/1‑94 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302536.4382999/1‑94 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302536.3250138/1‑94 (MQ=60)
                                                                            ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  <  SRR4302536.5388013/94‑1 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 >  SRR4302536.4869684/1‑94 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 >  SRR4302536.4581894/1‑94 (MQ=60)
                                                                                 CGTTATCCCCGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302536.6738906/1‑94 (MQ=60)
                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302536.4395485/1‑94 (MQ=60)
                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302536.6389146/94‑1 (MQ=60)
                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302536.503269/94‑1 (MQ=60)
                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302536.6909265/1‑94 (MQ=60)
                                                                                 CGTTACCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302536.3749373/94‑1 (MQ=60)
                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            <  SRR4302536.1130780/94‑1 (MQ=60)
                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            <  SRR4302536.6273744/94‑1 (MQ=60)
                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            >  SRR4302536.611065/1‑94 (MQ=60)
                                                                                     ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         >  SRR4302536.4558737/1‑94 (MQ=60)
                                                                                     ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         <  SRR4302536.2117989/94‑1 (MQ=60)
                                                                                      TCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGT        <  SRR4302536.6461329/94‑1 (MQ=60)
                                                                                       CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       >  SRR4302536.2990392/1‑94 (MQ=60)
                                                                                        CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      >  SRR4302536.710115/1‑94 (MQ=60)
                                                                                        CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302536.3153028/94‑1 (MQ=60)
                                                                                          AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA    <  SRR4302536.6631230/94‑1 (MQ=60)
                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302536.6801291/1‑94 (MQ=60)
                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302536.6441413/1‑94 (MQ=60)
                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302536.6043914/1‑94 (MQ=60)
                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  <  SRR4302536.944184/94‑1 (MQ=60)
                                                                                            |                                                                                             
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑