Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,170,345 C→T G184E (GGA→GAA)  gatD ← galactitol‑1‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,170,3450CT97.6% 728.7 / ‑1.4 211G184E (GGA→GAA) gatDgalactitol‑1‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (103/103);  total (105/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434
                                                                                          |                                                                                         
tCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTtcc                                                                                         <  1:3323570/93‑1 (MQ=255)
 cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                        <  2:2332164/93‑1 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      >  2:1841164/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      <  1:4249737/93‑1 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      >  1:3008931/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      <  2:2719583/93‑1 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      <  2:942481/93‑1 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  2:584182/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     <  2:2805421/93‑1 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     <  2:3489773/93‑1 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  1:4473600/93‑1 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  1:693670/93‑1 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTTCTTCCAGCGCGACa                                                                                <  1:807397/93‑1 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                                >  2:2742582/1‑93 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               <  1:205370/93‑1 (MQ=255)
           aaaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgc                                                                              <  1:2883205/93‑1 (MQ=255)
            aaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgcg                                                                             >  2:300990/1‑93 (MQ=255)
              tGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCa                                                                           <  1:3778030/93‑1 (MQ=255)
               gTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAc                                                                          >  2:4398690/1‑93 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  2:2179151/93‑1 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  2:3432367/93‑1 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  1:877299/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    >  2:341175/1‑93 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   >  2:133776/1‑93 (MQ=255)
                       cGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGc                                                                  <  2:4296769/93‑1 (MQ=255)
                           ccGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagc                                                              >  1:4606030/1‑93 (MQ=255)
                           acGAAAGATTGTGGCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagc                                                              <  1:3212860/92‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  2:1374180/93‑1 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  1:1442980/1‑93 (MQ=255)
                              aaaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAgg                                                           >  1:316029/1‑93 (MQ=255)
                              aaaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAgg                                                           >  2:1183563/1‑93 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCTCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          >  1:102470/1‑93 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          <  1:1799757/93‑1 (MQ=255)
                               aaGATTTTGACAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          >  1:4675560/1‑93 (MQ=255)
                                 aaTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        <  2:969601/92‑1 (MQ=255)
                                   ttttGCCAGTGCCAGTTTTTCTGAACTAATGTTGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      <  1:2872524/93‑1 (MQ=255)
                                   ttttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      >  2:1603666/1‑93 (MQ=255)
                                    tttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATg                                                     <  2:2397987/93‑1 (MQ=255)
                                       gccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGtt                                                  <  1:2353132/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGTTTc                                                 <  2:4518361/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                >  1:3221212/1‑93 (MQ=255)
                                          cgtgccagtTTTTCTGAACTAATGTGGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg                                               <  2:2798206/92‑1 (MQ=255)
                                          agtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg                                               >  1:4028210/1‑93 (MQ=255)
                                           gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCGAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                              >  1:1004702/1‑93 (MQ=255)
                                           gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCTCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                              <  2:897573/93‑1 (MQ=255)
                                            ttccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGc                                             <  2:3996733/91‑1 (MQ=255)
                                              ccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAc                                           >  1:2023767/1‑93 (MQ=255)
                                              ccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAc                                           <  1:2441359/93‑1 (MQ=255)
                                                 gtTTTTCTGAACTAATGTCGATCGCCGTCACACTATTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaa                                        <  2:715556/93‑1 (MQ=255)
                                                  tttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaat                                       <  2:3175635/93‑1 (MQ=255)
                                                     ttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataat                                    <  1:308988/93‑1 (MQ=255)
                                                      tCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataata                                   <  2:1012079/93‑1 (MQ=255)
                                                      tCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataata                                   >  1:1484342/1‑93 (MQ=255)
                                                      tCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataata                                   <  2:3827713/93‑1 (MQ=255)
                                                      tCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataata                                   >  1:3442598/1‑93 (MQ=255)
                                                       cTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataa                                  >  2:4093731/1‑93 (MQ=255)
                                                       cTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataa                                  <  1:570238/93‑1 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               <  2:2066518/93‑1 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              >  2:1927403/1‑93 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              >  1:637263/1‑93 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           <  1:2600438/93‑1 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           >  1:96852/1‑93 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          <  1:1564531/93‑1 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         >  2:89627/1‑93 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  2:2015489/93‑1 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        >  2:4227590/1‑93 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  1:105243/93‑1 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  1:3851545/93‑1 (MQ=255)
                                                                   cGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAt                      >  2:2928988/1‑93 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     >  2:3807846/1‑93 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     >  1:2277364/1‑93 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     >  2:4116794/1‑93 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCTCACTGAATGACCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  2:2638040/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  2:1651943/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  1:3890540/93‑1 (MQ=255)
                                                                      tCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtct                   <  2:3216322/93‑1 (MQ=255)
                                                                        gCCGTCACATTCTTTGCTTGCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 <  1:2709185/93‑1 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 >  2:2293808/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                <  2:3888188/93‑1 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                <  1:3200695/93‑1 (MQ=255)
                                                                          cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca               <  2:2837898/93‑1 (MQ=255)
                                                                           gTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa              <  1:866648/93‑1 (MQ=255)
                                                                           gTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa              <  1:214273/93‑1 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             <  1:1081947/93‑1 (MQ=255)
                                                                             cacaCTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAcc            >  1:173878/1‑93 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt           <  2:1771072/93‑1 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGGTTTTATTCTCACAACCt           >  2:3101177/1‑93 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCCCTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          <  1:2563026/93‑1 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          >  1:2913094/1‑93 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          >  2:189013/1‑93 (MQ=255)
                                                                               cacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGACAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCtt          >  2:965077/1‑93 (MQ=255)
                                                                                acTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTg         >  1:1068510/1‑93 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        <  2:2045889/93‑1 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        >  1:2088788/1‑93 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        >  1:203025/1‑93 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        <  1:1191366/93‑1 (MQ=255)
                                                                                  tctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcg       <  2:1474714/93‑1 (MQ=255)
                                                                                    tttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCt     <  2:4161556/93‑1 (MQ=255)
                                                                                     ttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTa    >  1:139963/1‑93 (MQ=255)
                                                                                       gCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTaaa  <  2:3636690/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170252‑2170438
                                                                                             |                                                                                             
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                               >  SRR4302544.3974494/1‑94 (MQ=60)
  TTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCC                                                                                             <  SRR4302544.612241/94‑1 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                            <  SRR4302544.3355225/94‑1 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                            >  SRR4302544.3465089/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                          >  SRR4302544.3037773/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         <  SRR4302544.4293067/94‑1 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                         >  SRR4302544.2117948/1‑94 (MQ=60)
          TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                     <  SRR4302544.2603952/94‑1 (MQ=60)
          TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                     >  SRR4302544.3938545/1‑94 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    <  SRR4302544.4518591/94‑1 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                    <  SRR4302544.700579/94‑1 (MQ=60)
            TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTTCTTCCAGCGCGACAG                                                                                   <  SRR4302544.815271/94‑1 (MQ=60)
            TTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAG                                                                                   <  SRR4302544.3893444/94‑1 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                  <  SRR4302544.207465/94‑1 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 >  SRR4302544.3270148/1‑94 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 <  SRR4302544.2911016/94‑1 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                 >  SRR4302544.4462451/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                                >  SRR4302544.4682781/1‑94 (MQ=60)
                 TGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAC                                                                              <  SRR4302544.3815461/94‑1 (MQ=60)
                 TGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAC                                                                              <  SRR4302544.2910211/94‑1 (MQ=60)
                   TGTGCAGCGCACCGAAAGATTTTGACAGTGCCAGTTTTTCGGACCTAACGTCGGTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCTCACTG                                                                            <  SRR4302544.1953854/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                          <  SRR4302544.204781/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         <  SRR4302544.827191/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         >  SRR4302544.1744840/1‑94 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                         <  SRR4302544.3176603/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        <  SRR4302544.886005/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                        >  SRR4302544.4640757/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       <  SRR4302544.1176427/94‑1 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                       >  SRR4302544.2683966/1‑94 (MQ=60)
                          CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCC                                                                     >  SRR4302544.4463319/1‑94 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302544.1288354/1‑94 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                  >  SRR4302544.4651795/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 <  SRR4302544.3243503/94‑1 (MQ=60)
                              ACGAAAGATTGTGGCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                 <  SRR4302544.3243502/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                >  SRR4302544.2441975/1‑94 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                                >  SRR4302544.1457338/1‑94 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                               >  SRR4302544.319312/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCTCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                              >  SRR4302544.103406/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                              >  SRR4302544.103405/1‑94 (MQ=60)
                                 AAAGATTTTGACAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                              >  SRR4302544.4721770/1‑94 (MQ=60)
                                  AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                             <  SRR4302544.1817347/94‑1 (MQ=60)
                                   AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                            >  SRR4302544.1813377/1‑94 (MQ=60)
                                      TTTTGCCAGTGCCAGTTTTTCTGAACTAATGTTGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATG                                                         <  SRR4302544.2900249/94‑1 (MQ=60)
                                        TTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGT                                                       >  SRR4302544.2927837/1‑94 (MQ=60)
                                          GCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTC                                                     <  SRR4302544.2375426/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    >  SRR4302544.3251959/1‑94 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                    >  SRR4302544.2941485/1‑94 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   >  SRR4302544.4068334/1‑94 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                   <  SRR4302544.2218815/94‑1 (MQ=60)
                                             AGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCGAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGG                                                  >  SRR4302544.1014685/1‑94 (MQ=60)
                                               TGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCA                                                >  SRR4302544.999389/1‑94 (MQ=60)
                                                GCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAC                                               >  SRR4302544.2043397/1‑94 (MQ=60)
                                                 CCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACC                                              <  SRR4302544.2464639/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                             <  SRR4302544.3857303/94‑1 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            >  SRR4302544.1229597/1‑94 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                            <  SRR4302544.1416534/94‑1 (MQ=60)
                                                     TTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATA                                          <  SRR4302544.769021/94‑1 (MQ=60)
                                                       TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                        <  SRR4302544.3476169/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       <  SRR4302544.3240570/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       >  SRR4302544.1499009/1‑94 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       <  SRR4302544.312200/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       <  SRR4302544.3795959/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                       >  SRR4302544.3475501/1‑94 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                     <  SRR4302544.575812/94‑1 (MQ=60)
                                                          CTGAACTAATGTCGATCGACGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCACTAATAAT                                     >  SRR4302544.426410/1‑94 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                    <  SRR4302544.1052802/94‑1 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                    >  SRR4302544.715623/1‑94 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                   >  SRR4302544.3118091/1‑94 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  >  SRR4302544.643542/1‑94 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                  <  SRR4302544.2104616/94‑1 (MQ=60)
                                                                TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                               >  SRR4302544.97738/1‑94 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              <  SRR4302544.2625434/94‑1 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                              <  SRR4302544.650573/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             <  SRR4302544.1580047/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                             >  SRR4302544.2869319/1‑94 (MQ=60)
                                                                   TGTCGATCGCCGTCACCCTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                            <  SRR4302544.966985/94‑1 (MQ=60)
                                                                    GTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTA                           <  SRR4302544.3889774/94‑1 (MQ=60)
                                                                    GTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTA                           <  SRR4302544.106211/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGTAGGCCAATGGTTCCGGCACCCATAATAATAACGTTTTTCT                          >  SRR4302544.2241567/1‑94 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGCCAGCGCACTGAATGGCCACCAGGCCAATGGTTCAGGCACCAATACTAATAACGTTTTTATT                         >  SRR4302544.1074602/1‑94 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         >  SRR4302544.4742900/1‑94 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                         >  SRR4302544.2298806/1‑94 (MQ=60)
                                                                        ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                       <  SRR4302544.3929183/94‑1 (MQ=60)
                                                                          CGCCGTCACACTCCTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                     >  SRR4302544.3183376/1‑94 (MQ=60)
                                                                           GCCGTCACATTCTTTGCTTGCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                    <  SRR4302544.2735236/94‑1 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                   <  SRR4302544.3231195/94‑1 (MQ=60)
                                                                             CGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAA                  >  SRR4302544.3204881/1‑94 (MQ=60)
                                                                             CGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAA                  >  SRR4302544.2051993/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                 <  SRR4302544.216474/94‑1 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                 <  SRR4302544.875223/94‑1 (MQ=60)
                                                                               TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACC                >  SRR4302544.2563249/1‑94 (MQ=60)
                                                                               TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACC                >  SRR4302544.175592/1‑94 (MQ=60)
                                                                               TCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACC                <  SRR4302544.1092708/94‑1 (MQ=60)
                                                                                CACACTCTTTGCTTCCAGCGCGACAGCGCCGTGAATGGGCAGTAGGCCAATGGTTCCGGCTCCAATAATAATAACGTTTTTATTCTCACAACCT               <  SRR4302544.1623985/94‑1 (MQ=60)
                                                                                CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               >  SRR4302544.1483458/1‑94 (MQ=60)
                                                                                CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               <  SRR4302544.2024379/94‑1 (MQ=60)
                                                                                CACACTCTGTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT               <  SRR4302544.2024378/94‑1 (MQ=60)
                                                                                 ACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTT              >  SRR4302544.2941116/1‑94 (MQ=60)
                                                                                  CACTCTTTGCTTCCAGCGCGACAGCGCCCTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             <  SRR4302544.2587653/94‑1 (MQ=60)
                                                                                  CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             <  SRR4302544.1089579/94‑1 (MQ=60)
                                                                                  CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG             >  SRR4302544.1079129/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            >  SRR4302544.2108891/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            >  SRR4302544.2821353/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            >  SRR4302544.205090/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC            >  SRR4302544.1519200/1‑94 (MQ=60)
                                                                                    CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG           <  SRR4302544.1203186/94‑1 (MQ=60)
                                                                                    CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG           <  SRR4302544.87734/94‑1 (MQ=60)
                                                                                    CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG           >  SRR4302544.589424/1‑94 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA        >  SRR4302544.2306576/1‑94 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA        >  SRR4302544.141289/1‑94 (MQ=60)
                                                                                          GCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAtaataaaggttttttttctacaaactttccgttaaag     >  SRR4302544.1331543/1‑57 (MQ=60)
                                                                                          GCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAAT     >  SRR4302544.1331405/1‑94 (MQ=60)
                                                                                           CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATG    >  SRR4302544.2959103/1‑94 (MQ=60)
                                                                                           CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATA    >  SRR4302544.3675778/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA   >  SRR4302544.1861690/1‑94 (MQ=60)
                                                                                             TCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  <  SRR4302544.3547195/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAA  >  NC_000913/2170252‑2170438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑