Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,170,345 C→T G184E (GGA→GAA)  gatD ← galactitol‑1‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,170,3450CT99.2% 851.7 / ‑5.3 236G184E (GGA→GAA) gatDgalactitol‑1‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (98/136);  total (99/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434
                                                                                          |                                                                                         
tCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTtcc                                                                                         <  1:3006917/93‑1 (MQ=255)
 cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                        >  2:5067822/1‑93 (MQ=255)
 cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                        >  2:3928746/1‑93 (MQ=255)
 cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                        >  1:3784292/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      <  1:1490494/93‑1 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:1118636/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  2:868329/1‑93 (MQ=255)
     tcTGTTAAATGTTTGCATCGCACCGACAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcgc                                                                                    <  2:6721335/92‑1 (MQ=255)
      cTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGcgcg                                                                                   >  2:3699095/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:2106462/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:1600662/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  1:1290045/1‑93 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  2:1157424/93‑1 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  2:4302189/93‑1 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                                >  2:5624388/1‑93 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                                >  2:4967632/1‑93 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               >  2:5299383/1‑93 (MQ=255)
            aaTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgcg                                                                             >  1:4661105/1‑93 (MQ=255)
              tGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCa                                                                           >  2:4515804/1‑93 (MQ=255)
                  tGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGa                                                                       <  1:6692338/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  2:1661090/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  2:2256594/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  2:2667133/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  1:5140928/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      >  2:5243064/1‑93 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  1:3116630/93‑1 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  1:1961973/93‑1 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  2:774479/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    <  1:3533404/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    <  2:3086783/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    >  2:164047/1‑93 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   >  1:4369366/1‑93 (MQ=255)
                        gCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGcc                                                                 <  2:2456972/93‑1 (MQ=255)
                        gCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGcc                                                                 >  1:2623899/1‑93 (MQ=255)
                         cACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCca                                                                <  2:5819059/93‑1 (MQ=255)
                           ccGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagc                                                              <  1:4324996/93‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  1:2571094/93‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  1:2173451/93‑1 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  2:1348856/1‑93 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          <  2:4045727/93‑1 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          <  1:4506619/93‑1 (MQ=255)
                                aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                         <  2:4721734/93‑1 (MQ=255)
                                aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                         >  1:6326160/1‑93 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        >  1:1457963/1‑93 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       <  1:6182533/93‑1 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       >  2:2294660/1‑93 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       >  2:1178606/1‑93 (MQ=255)
                                   ttttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      <  2:5666413/93‑1 (MQ=255)
                                   ttttGCCAGTGCCAGTTTTGCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAt                                                      <  2:5053323/93‑1 (MQ=255)
                                    tttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATg                                                     <  2:3635167/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  1:4734825/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                <  2:2089350/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                >  2:2900441/1‑93 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                >  1:552571/1‑93 (MQ=255)
                                           gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                              >  2:2615081/1‑93 (MQ=255)
                                           gtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCgg                                              <  1:1095640/93‑1 (MQ=255)
                                            tggaagtTTTTCTGAACTAATGTCGATCGCCGTCACAATACTTGCTTCCAGTGCGACAGCGCACTGACTGGCCAGCAGGCCAATGGTTCAGGc                                             >  1:2241501/5‑93 (MQ=255)
                                             gccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCa                                            <  2:2870743/93‑1 (MQ=255)
                                               cagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAcc                                          >  2:5886849/1‑93 (MQ=255)
                                               cagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAcc                                          <  1:622683/93‑1 (MQ=255)
                                                agtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCa                                         <  1:485823/93‑1 (MQ=255)
                                                    tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                     >  1:1705363/1‑93 (MQ=255)
                                                     ttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataat                                    <  1:4102297/93‑1 (MQ=255)
                                                     ttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataat                                    <  1:3323639/93‑1 (MQ=255)
                                                       cTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataa                                  <  2:1290045/93‑1 (MQ=255)
                                                       cTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataa                                  <  1:826144/93‑1 (MQ=255)
                                                         gAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAataataata                                <  1:2706470/93‑1 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               <  1:2528923/93‑1 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               <  2:5963216/93‑1 (MQ=255)
                                                             tAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGt                            >  2:6664010/1‑93 (MQ=255)
                                                             tAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAAGt                            >  2:5945804/1‑93 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           >  2:4031880/1‑93 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          <  1:5913862/93‑1 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          >  2:1758163/1‑93 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  1:3275386/93‑1 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         >  1:2905040/1‑93 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         >  1:4908857/1‑93 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  1:343513/93‑1 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        >  2:4787156/1‑93 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  2:2392480/93‑1 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     >  1:398218/1‑93 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  1:4948479/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  1:2335942/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  2:6461751/93‑1 (MQ=255)
                                                                     aTCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtc                    <  2:3175529/93‑1 (MQ=255)
                                                                      tCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtct                   <  1:3643432/93‑1 (MQ=255)
                                                                       cGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtctc                  >  2:1476592/1‑93 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 <  1:2842678/93‑1 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 >  1:4567442/1‑93 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 <  1:5774382/93‑1 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                <  2:2575279/93‑1 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  1:3882590/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  2:6308856/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  2:6385716/1‑93 (MQ=255)
                                                                          cGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcaca               <  2:2432814/93‑1 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             <  2:5437787/93‑1 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             >  1:654689/1‑93 (MQ=255)
                                                                             cacaCTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAcc            <  2:3260963/93‑1 (MQ=255)
                                                                                   ctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcgc      >  1:4447102/1‑93 (MQ=255)
                                                                                       gCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTaca  <  1:4204197/93‑3 (MQ=255)
                                                                                          |                                                                                         
TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  >  NC_000913/2170252‑2170437
                                                                                             |                                                                                            
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                              <  SRR4302536.3720205/94‑1 (MQ=60)
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                              <  SRR4302536.5361015/94‑1 (MQ=60)
  TTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCC                                                                                            >  SRR4302536.2270230/1‑94 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                           <  SRR4302536.3095314/94‑1 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                           >  SRR4302536.4802938/1‑94 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                           <  SRR4302536.3721105/94‑1 (MQ=60)
   TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCA                                                                                           >  SRR4302536.3897199/1‑94 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                          <  SRR4302536.1716591/94‑1 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        <  SRR4302536.6059534/94‑1 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        >  SRR4302536.1152219/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        >  SRR4302536.4380880/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        <  SRR4302536.1534533/94‑1 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                       >  SRR4302536.4572223/1‑94 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                       <  SRR4302536.4852435/94‑1 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302536.464069/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302536.2169766/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302536.1328630/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302536.4458173/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302536.1647384/1‑94 (MQ=60)
          TGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAC                                                                                    >  SRR4302536.3133709/1‑94 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                   <  SRR4302536.1436869/94‑1 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                >  SRR4302536.5759289/1‑94 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                >  SRR4302536.4801251/1‑94 (MQ=60)
               AATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGC                                                                               <  SRR4302536.4997680/94‑1 (MQ=60)
                ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                              <  SRR4302536.3633031/94‑1 (MQ=60)
                ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                              >  SRR4302536.4687200/1‑94 (MQ=60)
                  GTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACT                                                                            <  SRR4302536.3263106/94‑1 (MQ=60)
                  GTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACT                                                                            <  SRR4302536.2975603/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         <  SRR4302536.6891100/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         <  SRR4302536.2137253/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                        <  SRR4302536.6510465/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                        <  SRR4302536.5294380/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                       >  SRR4302536.6509149/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                       <  SRR4302536.3207675/94‑1 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                       <  SRR4302536.2020966/94‑1 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                      >  SRR4302536.4499902/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                      <  SRR4302536.3639000/94‑1 (MQ=60)
                          CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCC                                                                    >  SRR4302536.2702613/1‑94 (MQ=60)
                            CACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAG                                                                  <  SRR4302536.1823664/94‑1 (MQ=60)
                             ACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGC                                                                 >  SRR4302536.844583/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                >  SRR4302536.3434214/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                <  SRR4302536.4454507/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               <  SRR4302536.2648417/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               <  SRR4302536.2238911/94‑1 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAcggcacctcaaagtcaacagg                                                              >  SRR4302536.2492745/1‑73 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                              >  SRR4302536.4640369/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                             >  SRR4302536.218491/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                             >  SRR4302536.5210661/1‑94 (MQ=60)
                                  AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                            >  SRR4302536.6514579/1‑94 (MQ=60)
                                  AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                            >  SRR4302536.2276118/1‑94 (MQ=60)
                                  AAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCC                                                            <  SRR4302536.4641828/94‑1 (MQ=60)
                                   AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                           >  SRR4302536.1501122/1‑94 (MQ=60)
                                   AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCACGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                           >  SRR4302536.5017787/1‑94 (MQ=60)
                                     ATTTTGCCAGTGCCATTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                         <  SRR4302536.6868275/94‑1 (MQ=60)
                                     ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                         <  SRR4302536.6366665/94‑1 (MQ=60)
                                     ATTTTGCCAGGGCCAGTTTTTCTGAACTAATTGCCATCGCCGTCACCCTCTTTGCTTCCAGCGCGACAGGGCACTGAATGGCCAGCAGGCCAAT                                                         <  SRR4302536.3060746/94‑1 (MQ=60)
                                       TTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGG                                                       <  SRR4302536.4096233/94‑1 (MQ=60)
                                         TGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTT                                                     <  SRR4302536.4252623/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   >  SRR4302536.4262532/1‑94 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302536.2771457/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302536.4877318/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   >  SRR4302536.566652/1‑94 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                  <  SRR4302536.5672145/94‑1 (MQ=60)
                                              gtggaAGTTTTTCTGAACTAATGTCGATCGCCGTCACAATACTTGCTTCCAGTGCGACAGCGCACTGACTGGCCAGCAGGCCAATGGTTCAGGC                                                >  SRR4302536.2309339/6‑94 (MQ=60)
                                              GTGCTAGTTTTGCTGAACTAATGGCGACCGACGTCAGACTCTTTGCTGCCAGTGCGACAGCGCACTAAATGGCCAGCAGGCCAATGGTTCCGGC                                                <  SRR4302536.3085029/94‑1 (MQ=53)
                                              GTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGC                                                <  SRR4302536.1128509/94‑1 (MQ=60)
                                              GTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGC                                                <  SRR4302536.141717/94‑1 (MQ=60)
                                                GCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCAC                                              <  SRR4302536.4159989/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                            <  SRR4302536.641173/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                            >  SRR4302536.2387574/1‑94 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                            <  SRR4302536.5459532/94‑1 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATAGCCAGCAGGCCAATGGTTCCGGCctca                                            >  SRR4302536.1272285/1‑90 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           <  SRR4302536.498428/94‑1 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           >  SRR4302536.4491318/1‑94 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACGCTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                          <  SRR4302536.3417207/94‑1 (MQ=60)
                                                      TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                        >  SRR4302536.1754699/1‑94 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                      <  SRR4302536.4224002/94‑1 (MQ=60)
                                                        TTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATA                                      <  SRR4302536.3421956/94‑1 (MQ=60)
                                                         TCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAA                                     <  SRR4302536.4079967/94‑1 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                    <  SRR4302536.850463/94‑1 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  <  SRR4302536.2787222/94‑1 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  >  SRR4302536.619347/1‑94 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                 <  SRR4302536.2605154/94‑1 (MQ=60)
                                                               TTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302536.2616817/94‑1 (MQ=60)
                                                               CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302536.5081408/94‑1 (MQ=60)
                                                               CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302536.2706558/94‑1 (MQ=60)
                                                               CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302536.3958304/94‑1 (MQ=60)
                                                                TAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                              <  SRR4302536.4720934/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            <  SRR4302536.1614279/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            <  SRR4302536.6090007/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302536.2990779/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302536.5056421/1‑94 (MQ=60)
                                                                   TGTCGATCGCCGTCCCACTCTTTGCTTCCAGCGCGACAGCGGACTGAATGGCCCGCAGGCCAATGGTTCCGGCTCCAATAATAATAACGTTTTT                           <  SRR4302536.6367187/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                           <  SRR4302536.114701/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                           <  SRR4302536.1530516/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                           <  SRR4302536.3371621/94‑1 (MQ=60)
                                                                   TGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTT                           <  SRR4302536.352627/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302536.2838170/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302536.6651205/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302536.3716276/94‑1 (MQ=60)
                                                                     GCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGTCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302536.1572728/94‑1 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                        >  SRR4302536.408756/1‑94 (MQ=60)
                                                                       GATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTC                       >  SRR4302536.2448615/1‑94 (MQ=60)
                                                                        ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                      <  SRR4302536.2406812/94‑1 (MQ=60)
                                                                        ATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCT                      <  SRR4302536.5097153/94‑1 (MQ=60)
                                                                         TCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTC                     <  SRR4302536.3752368/94‑1 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                    >  SRR4302536.4704501/1‑94 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                    >  SRR4302536.2427165/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                   <  SRR4302536.5946204/94‑1 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                   >  SRR4302536.6882883/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                   >  SRR4302536.3998080/1‑94 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                   <  SRR4302536.2926772/94‑1 (MQ=60)
                                                                           GCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAC                   >  SRR4302536.2989040/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                <  SRR4302536.3881016/94‑1 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                >  SRR4302536.673883/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC           <  SRR4302536.2684178/94‑1 (MQ=60)
                                                                                    CTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG          >  SRR4302536.3493501/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302536.904667/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302536.3258968/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302536.4580642/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         <  SRR4302536.6075369/94‑1 (MQ=60)
                                                                                      CTTTGCTTCCAGCGCGACAGCGCACTGAAGGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT        <  SRR4302536.2137365/94‑1 (MQ=60)
                                                                                        TTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAA      <  SRR4302536.5071411/94‑1 (MQ=60)
                                                                                          GCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTACAT    <  SRR4302536.4329169/94‑1 (MQ=60)
                                                                                           CTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATG   >  SRR4302536.3160155/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  >  SRR4302536.2803246/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  >  NC_000913/2170252‑2170437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑