Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,216,746	G→C	S252S (TCC→TCG)	yehZ ←	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,216,746	0	G	C	100.0%	450.4 / NA	138	S252S (TCC→TCG)	yehZ	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (61/77); total (61/77)

Only 100 of 148 total aligned reads displayed.

GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC  >  NC_000913/2216656‑2216835

  tGGCATTCAGTTGCTGCAATGGTTTTGCATCGAGGCTGGCGAAGAATAGCTGTAGCCACCGTTCCATTTGCCGATGGTCCCCCAACACCGACt                                                                                       >  2:3917810/1‑93 (MQ=255)

   ggCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                                      <  1:4676926/93‑1 (MQ=255)

   ggCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                                      >  1:393718/1‑93 (MQ=255)

   ggCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                                      >  2:3579299/1‑93 (MQ=255)

   ggCATTCAGTGGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                                      <  1:2519984/93‑1 (MQ=255)

    gCATTCATTTACTGCAATGTTTTTGCATCGAGGCTGTCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCa                                                                                     <  1:478584/93‑1 (MQ=255)

     cATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAc                                                                                    <  1:2414870/93‑1 (MQ=255)

      aTTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGCTACTCCCTCAACACCGACTCACg                                                                                   >  1:2260872/1‑93 (MQ=255)

        tCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGca                                                                                 >  2:3539803/1‑93 (MQ=255)

         cAGGTGCTGCAATGTTTTTGCATCCTGGCAGGAGTAAAGTGGCTGTGGACACCGGGACGTTTACAGGTACTTCCTGGACACCGACacacacac                                                                                >  2:4729998/1‑93 (MQ=255)

           gTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCCTTTGCGGATACTCCCTCAACACCGACTCCCGcccca                                                                              >  1:2861321/1‑93 (MQ=255)

           gTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacca                                                                              >  1:3709231/1‑93 (MQ=255)

            ttGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccac                                                                             <  2:701617/93‑1 (MQ=255)

             tgctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTTCGGATACTCCCTCAACACCGACTCACGCACCACt                                                                            >  2:190213/1‑93 (MQ=255)

             tgctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACt                                                                            >  1:91509/1‑93 (MQ=255)

             tgctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACt                                                                            >  2:190212/1‑93 (MQ=255)

              gctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTg                                                                           <  2:899854/93‑1 (MQ=255)

               ctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTgg                                                                          <  2:4073573/93‑1 (MQ=255)

                tgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGt                                                                         <  2:2579299/93‑1 (MQ=255)

                 gcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTg                                                                        <  1:948564/93‑1 (MQ=255)

                  cAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc                                                                       <  2:104320/93‑1 (MQ=255)

                   caTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCa                                                                      <  1:1523454/92‑1 (MQ=255)

                   aaTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACCGGCGCa                                                                      >  1:3986225/1‑93 (MQ=255)

                    aTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCTACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGTACCACTGGTGCAg                                                                     <  1:3135547/93‑1 (MQ=255)

                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                    >  1:4218548/1‑93 (MQ=255)

                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                    <  2:1103569/93‑1 (MQ=255)

                      gTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgc                                                                   >  2:3500336/1‑93 (MQ=255)

                         tttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                                >  2:1233337/1‑93 (MQ=255)

                          ttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGt                                                               <  1:3947573/93‑1 (MQ=255)

                            gCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg                                                             >  2:1215746/1‑93 (MQ=255)

                            gCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg                                                             >  2:3547485/1‑93 (MQ=255)

                            gCATCGAGGCTGGCAAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg                                                             <  2:2220026/93‑1 (MQ=255)

                            gCAGCGAGGCTGGGAAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAg                                                             <  2:14085/93‑1 (MQ=255)

                             cATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGa                                                            <  1:2551447/93‑1 (MQ=255)

                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           <  1:3515365/93‑1 (MQ=255)

                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                           <  1:4702764/93‑1 (MQ=255)

                               tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                          <  2:1993219/93‑1 (MQ=255)

                                cGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg                                                         <  2:806774/93‑1 (MQ=255)

                                 gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAgg                                                        <  2:4029001/93‑1 (MQ=255)

                                 gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAgg                                                        >  1:2027556/1‑93 (MQ=255)

                                 gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAAGCACCACTGGTGCAGGCGCGTAGATAgg                                                        >  1:2334063/1‑93 (MQ=255)

                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                       >  1:748485/1‑93 (MQ=255)

                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                      <  1:1917217/93‑1 (MQ=255)

                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                      <  2:3222641/93‑1 (MQ=255)

                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                      <  2:785627/93‑1 (MQ=255)

                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   >  1:4514473/1‑93 (MQ=255)

                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   <  1:2875563/93‑1 (MQ=255)

                                      tGGCGAACACTGGCTGTATCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                   <  2:2276596/93‑1 (MQ=255)

                                       ggCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCCACCACTGGTGCAGGCGCGTAGATACGTTGcac                                                  >  1:2901724/1‑93 (MQ=255)

                                        gCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcaca                                                 <  2:1649829/93‑1 (MQ=255)

                                         cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                                                >  2:453830/1‑93 (MQ=255)

                                         cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                                                >  1:3098648/1‑93 (MQ=255)

                                         cGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                                                >  1:4705392/1‑93 (MQ=255)

                                          gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc                                               <  2:1720609/93‑1 (MQ=255)

                                           aaGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCt                                              <  1:4171314/93‑1 (MQ=255)

                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             <  1:1264581/93‑1 (MQ=255)

                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             <  2:2676950/93‑1 (MQ=255)

                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             >  1:1585417/1‑93 (MQ=255)

                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                             >  1:3221117/1‑93 (MQ=255)

                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCttc                                            >  2:4423156/1‑92 (MQ=255)

                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                            >  1:265132/1‑93 (MQ=255)

                                              aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                           <  1:2188220/93‑1 (MQ=255)

                                               cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg                                          >  2:175859/1‑93 (MQ=255)

                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                        >  1:1486951/1‑93 (MQ=255)

                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                        <  1:2511137/93‑1 (MQ=255)

                                                  gCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                                       <  2:713957/93‑1 (MQ=255)

                                                  gCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                                       <  2:2136452/93‑1 (MQ=255)

                                                  gCTGTAGCCACTGTGCCATTTGCCGATCCTCCCTCAACACCGACTCACGAACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                                       <  1:530470/93‑1 (MQ=255)

                                                   cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                      >  2:1797340/1‑93 (MQ=255)

                                                    tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCg                                     >  1:2932939/1‑93 (MQ=255)

                                                    tGTAGCCACTCTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCg                                     <  2:2336662/93‑1 (MQ=255)

                                                     gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGc                                    >  2:3768703/1‑93 (MQ=255)

                                                      tAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCt                                   <  2:1603852/93‑1 (MQ=255)

                                                          cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg                               <  2:845814/93‑1 (MQ=255)

                                                            cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                             <  1:2247424/93‑1 (MQ=255)

                                                            cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt                             <  1:157104/93‑1 (MQ=255)

                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                            >  1:4502880/1‑93 (MQ=255)

                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                            <  2:4170570/93‑1 (MQ=255)

                                                              gtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt                           <  2:3478461/93‑1 (MQ=255)

                                                                  cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAg                       <  1:2737850/93‑1 (MQ=255)

                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                     >  2:4283152/1‑93 (MQ=255)

                                                                     ttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc                    <  1:3631384/93‑1 (MQ=255)

                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc                   <  1:840881/93‑1 (MQ=255)

                                                                       gCGGATACTCCCTCACCACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCa                  <  1:991803/93‑1 (MQ=255)

                                                                       gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCa                  <  2:2492454/93‑1 (MQ=255)

                                                                       gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCa                  >  2:4386998/1‑93 (MQ=255)

                                                                       gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCa                  <  2:518661/93‑1 (MQ=255)

                                                                           aTACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcgc              >  1:602203/1‑93 (MQ=255)

                                                                               tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCt          >  2:4582474/1‑93 (MQ=255)

                                                                                cccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTa         >  2:2444189/1‑93 (MQ=255)

                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc        <  2:3766965/93‑1 (MQ=255)

                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc        <  2:300067/93‑1 (MQ=255)

                                                                                  cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAcc       <  1:4035751/93‑1 (MQ=255)

                                                                                   tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg      <  2:2021694/93‑1 (MQ=255)

                                                                                   tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg      >  1:2119212/1‑93 (MQ=255)

                                                                                    cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCgg     <  1:3464842/93‑1 (MQ=255)

                                                                                    cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCgg     <  1:2327523/93‑1 (MQ=255)

                                                                                      acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGc   <  2:3925345/93‑1 (MQ=255)

                                                                                      acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGc   >  1:1217777/1‑93 (MQ=255)

                                                                                       cacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACGCCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGcc  <  1:1025503/93‑1 (MQ=255)

GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCC  >  NC_000913/2216656‑2216835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               <  SRR4302544.3995620/94‑1 (MQ=60)

   GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGAC                                                                                            <  SRR4302544.2095566/94‑1 (MQ=60)

     TGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTC                                                                                          >  SRR4302544.397709/1‑94 (MQ=60)

      GGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCA                                                                                         <  SRR4302544.4723154/94‑1 (MQ=60)

      GGCATTCAGTGGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCA                                                                                         <  SRR4302544.2544194/94‑1 (MQ=60)

       GCATTCATTTACTGCAATGTTTTTGCATCGAGGCTGTCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAC                                                                                        <  SRR4302544.483274/94‑1 (MQ=60)

       GCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAC                                                                                        >  SRR4302544.2242757/1‑94 (MQ=60)

        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGCTACTCCCTCAACACCGACTCACG                                                                                       >  SRR4302544.2282143/1‑94 (MQ=60)

        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACG                                                                                       <  SRR4302544.2437846/94‑1 (MQ=60)

        CATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACG                                                                                       >  SRR4302544.2316470/1‑94 (MQ=60)

         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGC                                                                                      <  SRR4302544.4020544/94‑1 (MQ=60)

           TCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCAC                                                                                    <  SRR4302544.959729/94‑1 (MQ=60)

             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCCTTTGCGGATACTCCCTCAACACCGACTCCCGCCCCA                                                                                  >  SRR4302544.2888940/1‑94 (MQ=60)

             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA                                                                                  >  SRR4302544.3746128/1‑94 (MQ=60)

               TTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACT                                                                                >  SRR4302544.92364/1‑94 (MQ=60)

                    GCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGC                                                                           <  SRR4302544.957900/94‑1 (MQ=60)

                     CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACCGGCGCA                                                                          >  SRR4302544.4025907/1‑94 (MQ=60)

                      CATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAG                                                                         <  SRR4302544.1538570/94‑1 (MQ=60)

                      AATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAG                                                                         <  SRR4302544.1538571/94‑1 (MQ=60)

                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCTACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGTACCACTGGTGCAGG                                                                        <  SRR4302544.3165331/94‑1 (MQ=60)

                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGG                                                                        >  SRR4302544.4261230/1‑94 (MQ=60)

                            TTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGT                                                                   >  SRR4302544.3297778/1‑94 (MQ=60)

                             TTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTA                                                                  <  SRR4302544.3986798/94‑1 (MQ=60)

                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               <  SRR4302544.2575940/94‑1 (MQ=60)

                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302544.4487822/1‑94 (MQ=60)

                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               <  SRR4302544.2914728/94‑1 (MQ=60)

                                 atcggcgctgagGAAGACTGGCTGTAGCCACTGTGACATTTGCGGATACTCCCTCAACGCCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              <  SRR4302544.3548917/82‑1 (MQ=60)

                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              <  SRR4302544.3548918/94‑1 (MQ=60)

                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              <  SRR4302544.4749242/94‑1 (MQ=60)

                                  TCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAG                                                             <  SRR4302544.1928116/94‑1 (MQ=60)

                                  TCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAG                                                             <  SRR4302544.3638153/94‑1 (MQ=60)

                                  TCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAG                                                             >  SRR4302544.4716399/1‑94 (MQ=60)

                                   CGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGG                                                            >  SRR4302544.2047204/1‑94 (MQ=60)

                                   CGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAAGCACCACTGGTGCAGGCGCGTAGATAGG                                                            >  SRR4302544.2356191/1‑94 (MQ=60)

                                    GAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGT                                                           >  SRR4302544.755806/1‑94 (MQ=60)

                                      GGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTG                                                         <  SRR4302544.1935742/94‑1 (MQ=60)

                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302544.4559692/1‑94 (MQ=60)

                                         TGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCAC                                                      <  SRR4302544.2903317/94‑1 (MQ=60)

                                         TGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCCACCACTGGTGCAGGCGCGTAGATACGTTGCAC                                                      >  SRR4302544.2929668/1‑94 (MQ=60)

                                           GCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAC                                                    >  SRR4302544.3128153/1‑94 (MQ=60)

                                           GCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAC                                                    >  SRR4302544.4751887/1‑94 (MQ=60)

                                           GCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAC                                                    >  SRR4302544.4751888/1‑94 (MQ=60)

                                              AAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTT                                                 <  SRR4302544.4212637/94‑1 (MQ=60)

                                              AAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTT                                                 >  SRR4302544.3251864/1‑94 (MQ=60)

                                              AAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTT                                                 >  SRR4302544.1601070/1‑94 (MQ=60)

                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                <  SRR4302544.1277023/94‑1 (MQ=60)

                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                >  SRR4302544.267931/1‑94 (MQ=60)

                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                <  SRR4302544.2403862/94‑1 (MQ=60)

                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              >  SRR4302544.48417/1‑94 (MQ=60)

                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              <  SRR4302544.2208899/94‑1 (MQ=60)

                                                   TGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGA                                            >  SRR4302544.1501650/1‑94 (MQ=60)

                                                   TGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGA                                            >  SRR4302544.2991779/1‑94 (MQ=60)

                                                    GGCTGTAGCCACTTTGCCATTTGCGGATACTCCCTCAACCCCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                           <  SRR4302544.2535237/94‑1 (MQ=60)

                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                           <  SRR4302544.2535236/94‑1 (MQ=60)

                                                     GCTGTAGCCACTGTGCCATTTGCCGATCCTCCCTCAACACCGACTCACGAACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATC                                          <  SRR4302544.535731/94‑1 (MQ=60)

                                                      CTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCG                                         >  SRR4302544.2961122/1‑94 (MQ=60)

                                                            CCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAG                                   >  SRR4302544.712358/1‑94 (MQ=60)

                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  >  SRR4302544.4156257/1‑94 (MQ=60)

                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                <  SRR4302544.2268551/94‑1 (MQ=60)

                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                >  SRR4302544.4548052/1‑94 (MQ=60)

                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                <  SRR4302544.158643/94‑1 (MQ=60)

                                                                   GCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                            <  SRR4302544.1449832/94‑1 (MQ=60)

                                                                    CCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAG                           >  SRR4302544.2782414/1‑94 (MQ=60)

                                                                     CATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGC                          <  SRR4302544.2764237/94‑1 (MQ=60)

                                                                        TTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCC                       <  SRR4302544.3667565/94‑1 (MQ=60)

                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302544.849180/94‑1 (MQ=60)

                                                                          GCGGATACTCCCTCACCACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAG                     <  SRR4302544.1001594/94‑1 (MQ=60)

                                                                          GCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAG                     <  SRR4302544.2635527/94‑1 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCAGGCACCACCGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   >  SRR4302544.1463957/1‑94 (MQ=60)

                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTACACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   <  SRR4302544.3975906/94‑1 (MQ=60)

                                                                             GATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGC                  >  SRR4302544.608106/1‑94 (MQ=60)

                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC           <  SRR4302544.909926/94‑1 (MQ=60)

                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          >  SRR4302544.2139558/1‑94 (MQ=60)

                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          <  SRR4302544.4075945/94‑1 (MQ=60)

                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        <  SRR4302544.3497894/94‑1 (MQ=60)

                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        <  SRR4302544.2349588/94‑1 (MQ=60)

                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       >  SRR4302544.1229826/1‑94 (MQ=60)

                                                                                          CACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACGCCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG     <  SRR4302544.1035738/94‑1 (MQ=60)

                                                                                           ACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGT    >  SRR4302544.1744156/1‑94 (MQ=60)

                                                                                            CCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTC   >  SRR4302544.3981712/1‑94 (MQ=60)

                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  SRR4302544.45437/1‑94 (MQ=60)

                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  <  SRR4302544.4515736/94‑1 (MQ=60)

AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`