Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,223,632 A→G L15P (CTG→CCG)  yohC ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,6320AG97.7% 528.0 / ‑0.9 173L15P (CTG→CCG) yohChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (103/65);  total (105/68)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721
                                                                                          |                                                                                         
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  1:2882538/1‑93 (MQ=255)
  cATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcc                                                                                       >  1:1948536/1‑93 (MQ=255)
   aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      >  1:4523907/1‑93 (MQ=255)
   aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      >  1:1320063/1‑93 (MQ=255)
   aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      >  2:1877069/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  1:688894/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  1:705108/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     <  2:4548728/93‑1 (MQ=255)
      aGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAg                                                                                   >  1:98470/1‑93 (MQ=255)
       gCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGa                                                                                  <  1:1137121/93‑1 (MQ=255)
         aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  1:3883284/1‑93 (MQ=255)
         aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  2:3939548/1‑93 (MQ=255)
         aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATAGGGCTGGGAAACCGGTCCCCAGACa                                                                                >  1:643521/1‑93 (MQ=255)
          aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               <  2:1919655/93‑1 (MQ=255)
          aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               >  1:3532984/1‑93 (MQ=255)
           aaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATg                                                                              >  2:4327817/1‑93 (MQ=255)
            aCGTGGTGGGTGGAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGACCCCAGACATgg                                                                             >  2:4374560/1‑93 (MQ=255)
              gtggtgGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCt                                                                           >  1:2438298/1‑93 (MQ=255)
              gtggtgGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCt                                                                           >  2:3142624/1‑93 (MQ=255)
               tggtggGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTc                                                                          >  1:2555328/1‑93 (MQ=255)
               tggtggGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTc                                                                          <  2:610597/93‑1 (MQ=255)
               tggtggGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTc                                                                          >  2:1444622/1‑93 (MQ=255)
                   gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAc                                                                      <  1:163350/93‑1 (MQ=255)
                    ggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAcc                                                                     >  1:1090881/1‑93 (MQ=255)
                        tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccc                                                                 <  1:4096760/93‑1 (MQ=255)
                          aTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccccc                                                               >  1:3169400/1‑93 (MQ=255)
                          aTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccccc                                                               >  1:3189676/1‑93 (MQ=255)
                          aTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccccc                                                               >  1:3676499/1‑93 (MQ=255)
                          aTGGTGAGAAATCGTTTCGTTTTAGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccccc                                                               >  2:2566025/1‑93 (MQ=255)
                           tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCt                                                              <  2:4171460/93‑1 (MQ=255)
                           tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCt                                                              <  2:4682866/93‑1 (MQ=255)
                            ggTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAAAGGAGCCCAGACATGGCTCATACAGCCCCCtt                                                             >  1:733295/1‑93 (MQ=255)
                               gagaAATAGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          <  2:4490080/93‑1 (MQ=255)
                                agaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgttg                                                         <  1:1849987/93‑1 (MQ=255)
                                 gaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTtgttgt                                                        >  1:3624186/1‑93 (MQ=255)
                                 gaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTtgttgt                                                        >  1:447830/1‑93 (MQ=255)
                                  aaaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTtgtg                                                       >  2:1217747/1‑93 (MQ=255)
                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTtgtgg                                                      >  2:1470743/1‑92 (MQ=255)
                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  1:4349909/93‑1 (MQ=255)
                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  2:488000/93‑1 (MQ=255)
                                     tcgtttcgttTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGt                                                    <  2:934877/93‑1 (MQ=255)
                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGttt                                                  >  1:599285/1‑92 (MQ=255)
                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGtta                                                  >  1:782859/1‑92 (MQ=255)
                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  <  1:935242/93‑1 (MQ=255)
                                         ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                <  2:884620/93‑1 (MQ=255)
                                         ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                >  1:3790218/1‑93 (MQ=255)
                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             <  2:2922053/93‑1 (MQ=255)
                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  1:1232935/1‑93 (MQ=255)
                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            <  2:423902/93‑1 (MQ=255)
                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCGGGCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  1:2687740/1‑93 (MQ=255)
                                                tCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTg                                         >  2:2346333/1‑93 (MQ=255)
                                                  gcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       >  2:852827/1‑93 (MQ=255)
                                                  gcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       >  1:2045232/1‑93 (MQ=255)
                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  1:1083464/1‑93 (MQ=255)
                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  2:163551/1‑93 (MQ=255)
                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  2:1374269/1‑93 (MQ=255)
                                                       tAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTc                                  >  2:3582404/1‑93 (MQ=255)
                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  2:4024047/93‑1 (MQ=255)
                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               >  1:4702087/1‑93 (MQ=255)
                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGCTGTGAGTTCATGAACCTGACAGTTCAAg                               >  1:1847714/1‑93 (MQ=255)
                                                           cACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGt                              <  2:245455/93‑1 (MQ=255)
                                                           cACCTGCATTTCACCATCGGTGTGGGAACACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGt                              <  2:3384942/93‑1 (MQ=255)
                                                            cccTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             <  2:3858203/92‑1 (MQ=255)
                                                            aCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAAta                             <  1:1768325/93‑1 (MQ=255)
                                                             ccTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtat                            >  2:2862863/1‑93 (MQ=255)
                                                              cTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACGTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtata                           >  1:3052946/1‑93 (MQ=255)
                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  2:4114931/93‑1 (MQ=255)
                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          >  1:597190/1‑93 (MQ=255)
                                                                 cATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGt                        <  2:1070045/93‑1 (MQ=255)
                                                                   tttCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      >  2:2124591/1‑93 (MQ=255)
                                                                     tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    <  2:2395059/93‑1 (MQ=255)
                                                                        cGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtg                 <  2:670235/93‑1 (MQ=255)
                                                                        cGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtg                 >  2:1859338/1‑93 (MQ=255)
                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                >  2:4281428/1‑93 (MQ=255)
                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                >  1:3225691/1‑93 (MQ=255)
                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                <  1:3375977/93‑1 (MQ=255)
                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                >  2:2056077/1‑93 (MQ=255)
                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                <  2:3249752/93‑1 (MQ=255)
                                                                          aTCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgtg               >  1:793658/1‑93 (MQ=255)
                                                                           tCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTGAGCTtgtgt              >  2:3820897/1‑92 (MQ=255)
                                                                           tCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGa              <  1:2634938/93‑1 (MQ=255)
                                                                             gggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGatt            >  2:2362191/1‑93 (MQ=255)
                                                                              ggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattt           >  1:892450/1‑92 (MQ=255)
                                                                              ggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGatta           >  2:2434755/1‑93 (MQ=255)
                                                                               gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattat          <  2:3453669/93‑1 (MQ=255)
                                                                               gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattat          >  1:3599507/1‑93 (MQ=255)
                                                                                aTGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattatt         <  1:1884662/93‑1 (MQ=255)
                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATAGCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGGTCAAGTATAAGGCAGCTTGTGATTAttt        >  1:2543219/1‑93 (MQ=255)
                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttt        <  1:350526/93‑1 (MQ=255)
                                                                                   ggAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttttt      >  1:235438/1‑93 (MQ=255)
                                                                                   ggAAAACGGTCCCCAGACATGACTCATACCGCCCCCTTGTTCTGAGTTCATGACCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttttt      <  1:531722/93‑1 (MQ=255)
                                                                                    gAAAACTGTCCCCAGACATGTCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttt     <  1:1499505/93‑1 (MQ=255)
                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  2:3674235/93‑1 (MQ=255)
                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  1:244621/93‑1 (MQ=255)
                                                                                      aaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGt   <  2:215998/93‑1 (MQ=255)
                                                                                      aaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGt   >  2:3423443/1‑93 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  <  2:264765/93‑1 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  <  1:4307849/93‑1 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  <  1:120130/93‑1 (MQ=255)
                                                                                       aaCGGTCCCCAGACAGGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  <  2:264764/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  NC_000913/2223539‑2223725
                                                                                             |                                                                                             
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               >  SRR4302544.4551535/1‑94 (MQ=60)
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               >  SRR4302544.3427266/1‑94 (MQ=60)
 CCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGG                                                                                              >  SRR4302544.914164/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                             >  SRR4302544.2910343/1‑94 (MQ=60)
    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                           <  SRR4302544.611928/94‑1 (MQ=60)
    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                           >  SRR4302544.1967408/1‑94 (MQ=60)
     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                          >  SRR4302544.4569189/1‑94 (MQ=60)
     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                          >  SRR4302544.1333047/1‑94 (MQ=60)
     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                          >  SRR4302544.1896638/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                         >  SRR4302544.712119/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                         >  SRR4302544.695752/1‑94 (MQ=60)
        CAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                       >  SRR4302544.99372/1‑94 (MQ=60)
         AGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGA                                                                                      >  SRR4302544.2078995/1‑94 (MQ=60)
          GCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGAC                                                                                     <  SRR4302544.1148431/94‑1 (MQ=60)
           CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACA                                                                                    >  SRR4302544.1734197/1‑94 (MQ=60)
           CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACA                                                                                    >  SRR4302544.788582/1‑94 (MQ=60)
           CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACA                                                                                    >  SRR4302544.3921856/1‑94 (MQ=60)
           CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATAGGGCTGGGAAACCGGTCCCCAGACA                                                                                    >  SRR4302544.649880/1‑94 (MQ=60)
            AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                   >  SRR4302544.3566782/1‑94 (MQ=60)
            AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                   >  SRR4302544.3566781/1‑94 (MQ=60)
              AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGG                                                                                 <  SRR4302544.3313673/94‑1 (MQ=60)
              AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGG                                                                                 <  SRR4302544.3796376/94‑1 (MQ=60)
                CGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCT                                                                               >  SRR4302544.2461544/1‑94 (MQ=60)
                 GTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTC                                                                              >  SRR4302544.2579862/1‑94 (MQ=60)
                   GGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGGTGGGAAAACGGTCCCCAGACATGGCTCAA                                                                            >  SRR4302544.734843/1‑94 (MQ=60)
                      GGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACC                                                                         <  SRR4302544.164946/94‑1 (MQ=60)
                      GGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACC                                                                         >  SRR4302544.1101731/1‑94 (MQ=60)
                       GGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCG                                                                        <  SRR4302544.3080394/94‑1 (MQ=60)
                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACTATCGGGATGGGAAAAAGGTCCCCACACAGGGCTCATACCGCC                                                                      >  SRR4302544.324916/1‑94 (MQ=60)
                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                      >  SRR4302544.240199/1‑94 (MQ=60)
                          GTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCC                                                                     >  SRR4302544.700094/1‑94 (MQ=60)
                           TAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCC                                                                    <  SRR4302544.4137532/94‑1 (MQ=60)
                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   >  SRR4302544.3713168/1‑94 (MQ=60)
                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   >  SRR4302544.3220066/1‑94 (MQ=60)
                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   >  SRR4302544.3199560/1‑94 (MQ=60)
                              TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAAAGGAGCCCAGACATGGCTCATACAGCCCCCTT                                                                 >  SRR4302544.740463/1‑94 (MQ=60)
                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302544.3660296/1‑94 (MQ=60)
                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            <  SRR4302544.1867888/94‑1 (MQ=60)
                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302544.452286/1‑94 (MQ=60)
                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302544.4493825/1‑94 (MQ=60)
                                      AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                         <  SRR4302544.4394110/94‑1 (MQ=60)
                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTT                                                      >  SRR4302544.605155/1‑94 (MQ=60)
                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302544.1835847/1‑94 (MQ=60)
                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTA                                                      >  SRR4302544.790440/1‑94 (MQ=60)
                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCT                                                     >  SRR4302544.1154454/1‑94 (MQ=60)
                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                     <  SRR4302544.944456/94‑1 (MQ=60)
                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                    >  SRR4302544.3827745/1‑94 (MQ=60)
                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                    <  SRR4302544.91353/94‑1 (MQ=60)
                                              CGTTTTCGCGATTAATCATCTGCATTTCAAGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 <  SRR4302544.3530860/94‑1 (MQ=60)
                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 >  SRR4302544.2468905/1‑94 (MQ=60)
                                              CGTGTGCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 <  SRR4302544.3304039/94‑1 (MQ=60)
                                               GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                                >  SRR4302544.2662067/1‑94 (MQ=60)
                                               GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                                >  SRR4302544.1245131/1‑94 (MQ=60)
                                               GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCGGGCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                                >  SRR4302544.2713507/1‑94 (MQ=60)
                                                    CGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAC                                           >  SRR4302544.654631/1‑94 (MQ=60)
                                                    CGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAC                                           >  SRR4302544.2064948/1‑94 (MQ=60)
                                                     tagattaatatCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCAAGTCATGGCCCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACA                                          <  SRR4302544.440144/83‑1 (MQ=60)
                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                        >  SRR4302544.1094240/1‑94 (MQ=60)
                                                        ATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTT                                       >  SRR4302544.4647131/1‑94 (MQ=60)
                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                     <  SRR4302544.89683/94‑1 (MQ=60)
                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                     >  SRR4302544.2628332/1‑94 (MQ=60)
                                                           AATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAA                                    <  SRR4302544.3098771/94‑1 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                   >  SRR4302544.4748561/1‑94 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                   <  SRR4302544.2850256/94‑1 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGCTCAAG                                   >  SRR4302544.1865603/1‑94 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGCTGTGAGTTCATGAACCTGACAGTTCAAG                                   >  SRR4302544.1865602/1‑94 (MQ=60)
                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAACGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                 >  SRR4302544.1113878/1‑94 (MQ=60)
                                                               CCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                                <  SRR4302544.366276/94‑1 (MQ=60)
                                                               ACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                                >  SRR4302544.1216429/1‑94 (MQ=60)
                                                               ACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAATAT                                <  SRR4302544.1785634/94‑1 (MQ=60)
                                                                CCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACGTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATA                               >  SRR4302544.3082107/1‑94 (MQ=60)
                                                                CCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATA                               >  SRR4302544.524330/1‑94 (MQ=60)
                                                                 CTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAA                              >  SRR4302544.3877251/1‑94 (MQ=60)
                                                                 CTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAA                              >  SRR4302544.603043/1‑94 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                             <  SRR4302544.355573/94‑1 (MQ=60)
                                                                    CATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTC                           >  SRR4302544.1974409/1‑94 (MQ=60)
                                                                     ATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCA                          >  SRR4302544.969679/1‑94 (MQ=60)
                                                                     ATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCA                          <  SRR4302544.3463149/94‑1 (MQ=60)
                                                                      TTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAG                         >  SRR4302544.401715/1‑94 (MQ=60)
                                                                           CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGT                    >  SRR4302544.579870/1‑94 (MQ=60)
                                                                           CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGT                    >  SRR4302544.3256521/1‑94 (MQ=60)
                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   <  SRR4302544.1311930/94‑1 (MQ=60)
                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   >  SRR4302544.801340/1‑94 (MQ=60)
                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   <  SRR4302544.3408205/94‑1 (MQ=60)
                                                                              TCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGAT                 <  SRR4302544.2660191/94‑1 (MQ=60)
                                                                                GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTT               >  SRR4302544.901282/1‑94 (MQ=60)
                                                                                 GGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAT              >  SRR4302544.3634250/1‑94 (MQ=60)
                                                                                  GATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATT             >  SRR4302544.1631848/1‑94 (MQ=60)
                                                                                   ATGGGAAAACGGTCCCCAGACATGGCTCATAGCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGGTCAAGTATAAGGCAGCTTGTGATTATTT            >  SRR4302544.2567634/1‑94 (MQ=60)
                                                                                   ATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTT            >  SRR4302544.2567633/1‑94 (MQ=60)
                                                                                   ATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTT            <  SRR4302544.1902881/94‑1 (MQ=60)
                                                                                    TGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTT           <  SRR4302544.354120/94‑1 (MQ=60)
                                                                                     GGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTT          >  SRR4302544.237886/1‑94 (MQ=60)
                                                                                      GGAAAACGGTCCCCAGACATGACTCATACCGCCCCCTTGTTCTGAGTTCATGACCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT         <  SRR4302544.536996/94‑1 (MQ=60)
                                                                                       GAAAACTGTCCCCAGACATGTCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTG        <  SRR4302544.1514333/94‑1 (MQ=60)
                                                                                       GAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTG        <  SRR4302544.4562855/94‑1 (MQ=60)
                                                                                        AAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGT       <  SRR4302544.247174/94‑1 (MQ=60)
                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTC     <  SRR4302544.4274940/94‑1 (MQ=60)
                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTC     <  SRR4302544.4351767/94‑1 (MQ=60)
                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTC     <  SRR4302544.121222/94‑1 (MQ=60)
                                                                                           ACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG    <  SRR4302544.2014651/94‑1 (MQ=60)
                                                                                             GGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  SRR4302544.2958642/1‑94 (MQ=60)
                                                                                             GGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  SRR4302544.4698114/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  NC_000913/2223539‑2223725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑