Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,632	A→G	L15P (CTG→CCG)	yohC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,632	0	A	G	100.0%	751.9 / NA	234	L15P (CTG→CCG)	yohC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (130/104); total (130/104)

Only 100 of 242 total aligned reads displayed.

GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721

gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  1:4724481/1‑93 (MQ=255)

gccATCAGCAAAACGTGGTGGGTGTAATGGGGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  2:2663422/1‑93 (MQ=255)

 ccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTc                                                                                        >  1:2829269/1‑93 (MQ=255)

  cATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcc                                                                                       >  1:1792291/1‑93 (MQ=255)

   aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGTGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      <  1:3918235/93‑1 (MQ=255)

    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAATGCGTTTCGTTTTCGCGATTTATCGCCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     <  2:4200364/93‑1 (MQ=255)

     cAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCa                                                                                    >  2:1816314/1‑93 (MQ=255)

     aaGCAAAACGTGGTGGTTGTAATGGTGAGAAAACCTTTCGTTTTCGCGTTTAATCCCCTGCATTTCCCGATGGGGATGGGAAAACGGTCCCCa                                                                                    <  2:2615063/92‑1 (MQ=255)

      aGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAg                                                                                   <  1:3771176/93‑1 (MQ=255)

          aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               <  2:4282900/93‑1 (MQ=255)

          aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               >  1:3567153/1‑93 (MQ=255)

          aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               >  1:3501935/1‑93 (MQ=255)

           aaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCGAGACATg                                                                              <  1:1667918/93‑1 (MQ=255)

           aaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATg                                                                              >  2:6447551/1‑93 (MQ=255)

           aaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATg                                                                              >  2:4548009/1‑93 (MQ=255)

              gtggtgGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCt                                                                           >  2:4897605/1‑93 (MQ=255)

                  tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  2:4349931/93‑1 (MQ=255)

                  tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  2:4482226/93‑1 (MQ=255)

                  tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       >  2:3359872/1‑93 (MQ=255)

                   gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAc                                                                      <  2:3789901/93‑1 (MQ=255)

                    ggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAcc                                                                     <  1:3076438/93‑1 (MQ=255)

                      tgtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGc                                                                   >  1:2997931/1‑93 (MQ=255)

                      tgtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGc                                                                   >  2:2506183/1‑93 (MQ=255)

                        tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccc                                                                 <  1:6058756/93‑1 (MQ=255)

                         aaTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGcccc                                                                <  1:940280/93‑1 (MQ=255)

                         aaTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGcccc                                                                >  2:3251009/1‑93 (MQ=255)

                           tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCt                                                              <  1:6257183/93‑1 (MQ=255)

                           tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCt                                                              <  1:4908832/93‑1 (MQ=255)

                            ggTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGAGCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCtt                                                             <  1:6469715/93‑1 (MQ=255)

                               gagaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          >  2:1755351/1‑93 (MQ=255)

                               gagaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          <  2:53639/93‑1 (MQ=255)

                                 gaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTtgttgt                                                        >  2:2976814/1‑93 (MQ=255)

                                  aaaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTtgtg                                                       >  2:5766738/1‑93 (MQ=255)

                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      >  1:220429/1‑93 (MQ=255)

                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  2:3561405/93‑1 (MQ=255)

                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      >  1:2973244/1‑93 (MQ=255)

                                    aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     >  2:228764/1‑93 (MQ=255)

                                    aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     <  1:205563/93‑1 (MQ=255)

                                    aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     <  2:4806349/93‑1 (MQ=255)

                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  >  2:5883262/1‑93 (MQ=255)

                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  >  1:812979/1‑93 (MQ=255)

                                        tttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCa                                                 >  2:6080081/1‑93 (MQ=255)

                                           cgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                              <  1:3296456/93‑1 (MQ=255)

                                           cgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                              <  2:4760553/93‑1 (MQ=255)

                                           cgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                              <  1:3605648/93‑1 (MQ=255)

                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             >  1:1347963/1‑93 (MQ=255)

                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             <  1:1919204/93‑1 (MQ=255)

                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             >  2:1089241/1‑93 (MQ=255)

                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  2:2360070/1‑93 (MQ=255)

                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  2:1876749/1‑93 (MQ=255)

                                              tttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAcc                                           <  2:2161044/93‑1 (MQ=255)

                                                tCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTg                                         <  1:2763608/93‑1 (MQ=255)

                                                 cgcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGa                                        <  2:3686004/93‑1 (MQ=255)

                                                 cgcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGa                                        <  2:6184880/93‑1 (MQ=255)

                                                  gcgATTAATCACCTGCATTTGACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       >  2:3846232/1‑93 (MQ=255)

                                                  gcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       <  1:5024674/93‑1 (MQ=255)

                                                   cgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACa                                      >  1:355765/1‑93 (MQ=255)

                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGGAAACGGTCCCCAGCCATGGCTCATACCGCCCCCTTGGTGTGAGTTTATGAACCTGACaat                                    >  1:1749194/1‑91 (MQ=255)

                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  1:880517/1‑93 (MQ=255)

                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    <  1:3133543/93‑1 (MQ=255)

                                                     aTTAATCACCTGCATTCCACGATCGGGATGGGAAAACGGTCCCCAGACGTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    <  1:1292407/93‑1 (MQ=255)

                                                       tAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTc                                  >  2:1095881/1‑93 (MQ=255)

                                                       tAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTc                                  >  2:4172319/1‑93 (MQ=255)

                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  2:5572064/93‑1 (MQ=255)

                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 >  1:6311371/1‑93 (MQ=255)

                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  1:5994914/93‑1 (MQ=255)

                                                         aTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCaa                                <  1:6411708/93‑1 (MQ=255)

                                                         aTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCACCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCaa                                >  2:783937/1‑93 (MQ=255)

                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               <  1:2437347/93‑1 (MQ=255)

                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               >  2:2236743/1‑93 (MQ=255)

                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               <  2:3975626/93‑1 (MQ=255)

                                                             ccTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtat                            <  1:3146936/93‑1 (MQ=255)

                                                              cttcATTTTACGATCGGGATGGGTAAACGGTTCCCTGACATTGCTCATACCGCCTCCTTGTTGTGTGTTCATGTACCTGACAGTTCAAGtata                           >  2:6719844/4‑93 (MQ=255)

                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  1:4849204/93‑1 (MQ=255)

                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          >  2:5052148/1‑93 (MQ=255)

                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          >  2:2828023/1‑93 (MQ=255)

                                                                   tttCACGATCGGGATGGGAAAACGGTCCCCAGTCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      >  1:5269661/1‑93 (MQ=255)

                                                                   tttCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      <  1:1239365/93‑1 (MQ=255)

                                                                   tttCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      <  1:2600676/93‑1 (MQ=255)

                                                                    ttCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAg                     >  2:6133171/1‑93 (MQ=255)

                                                                     tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    >  1:4295146/1‑93 (MQ=255)

                                                                     tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGATCAAGTATAAGTCAGc                    >  1:4696124/1‑93 (MQ=255)

                                                                      cACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATTAACCTGACAGTTCAAGTATAAGTCAGCt                   >  2:5176545/1‑93 (MQ=255)

                                                                        cGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtg                 >  2:1644962/1‑93 (MQ=255)

                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCTAGTATAAGTCAGCCtgt                >  1:5668825/1‑93 (MQ=255)

                                                                         gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                <  1:1493140/93‑1 (MQ=255)

                                                                           tCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGa              >  2:130997/1‑93 (MQ=255)

                                                                            cGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGat             <  1:5795887/93‑1 (MQ=255)

                                                                            cGGGATGGGAAAACGGACCCCAGACATGGCTCATACCGCCCCCTTGTGGTGAGTTCATGAACCGGACAGTTCAAGTATAAGTCAGCTTGTGat             <  1:3710916/93‑1 (MQ=255)

                                                                             gggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGatt            >  1:2029289/1‑93 (MQ=255)

                                                                              ggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGatta           >  1:764512/1‑93 (MQ=255)

                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttt        <  2:5230841/93‑1 (MQ=255)

                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttt        >  2:6287840/1‑93 (MQ=255)

                                                                                   ggAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttttt      <  1:6612713/93‑1 (MQ=255)

                                                                                    gAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttt     <  2:5681687/93‑1 (MQ=255)

                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  2:5028340/93‑1 (MQ=255)

                                                                                      aaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGACCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGt   <  1:5364410/93‑1 (MQ=255)

                                                                                      aaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGt   >  1:5901375/1‑93 (MQ=255)

                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  >  2:2928472/1‑93 (MQ=255)

                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  <  2:1211571/93‑1 (MQ=255)

GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  NC_000913/2223539‑2223725

GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               >  SRR4302536.727484/1‑94 (MQ=60)

GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               <  SRR4302536.3297979/94‑1 (MQ=60)

GCCACCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTCTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               <  SRR4302536.1866203/94‑1 (MQ=60)

CCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                               <  SRR4302536.5171339/94‑1 (MQ=60)

  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                             >  SRR4302536.4866626/1‑94 (MQ=60)

  CGCCATCAGCAAAACGGGGTGGGTGTAATGGGGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAACACGGT                                                                                             >  SRR4302536.6103837/1‑94 (MQ=60)

   GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTC                                                                                            >  SRR4302536.2913031/1‑94 (MQ=60)

    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                           >  SRR4302536.3826070/1‑94 (MQ=60)

    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                           >  SRR4302536.360363/1‑94 (MQ=60)

    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                           >  SRR4302536.1846418/1‑94 (MQ=60)

     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                          >  SRR4302536.3159501/1‑94 (MQ=60)

     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                          >  SRR4302536.1440600/1‑94 (MQ=60)

      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGTGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                         <  SRR4302536.4034706/94‑1 (MQ=60)

        CATCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                       <  SRR4302536.6107281/94‑1 (MQ=60)

        CAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                       <  SRR4302536.4897009/94‑1 (MQ=60)

         AGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGA                                                                                      >  SRR4302536.1832602/1‑94 (MQ=60)

         AGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGA                                                                                      <  SRR4302536.3883750/94‑1 (MQ=60)

            AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                   >  SRR4302536.3606432/1‑94 (MQ=60)

            AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                   >  SRR4302536.3673802/1‑94 (MQ=60)

              AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCGAGACATGG                                                                                 <  SRR4302536.1716309/94‑1 (MQ=60)

              AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGG                                                                                 <  SRR4302536.3625279/94‑1 (MQ=60)

                 GTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTC                                                                              >  SRR4302536.5628768/1‑94 (MQ=60)

                 GTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTC                                                                              >  SRR4302536.2167697/1‑94 (MQ=60)

                  TGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCA                                                                             >  SRR4302536.3418180/1‑94 (MQ=60)

                     TGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAC                                                                          <  SRR4302536.2725531/94‑1 (MQ=60)

                       GGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCG                                                                        <  SRR4302536.3341435/94‑1 (MQ=60)

                       GGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCG                                                                        <  SRR4302536.3166530/94‑1 (MQ=60)

                        GTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGC                                                                       >  SRR4302536.3086083/1‑94 (MQ=60)

                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                      >  SRR4302536.905857/1‑94 (MQ=60)

                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                      >  SRR4302536.6476367/1‑94 (MQ=60)

                         TGTAATGGTGAGAAATCGTTTCGTTATCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAGCGGTCCCCAGACATGGCTCATACCGCC                                                                      <  SRR4302536.1725847/94‑1 (MQ=60)

                          GTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCC                                                                     >  SRR4302536.3160041/1‑94 (MQ=60)

                           TAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCC                                                                    <  SRR4302536.6239499/94‑1 (MQ=60)

                           TAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCC                                                                    >  SRR4302536.6882079/1‑94 (MQ=60)

                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   >  SRR4302536.2234216/1‑94 (MQ=60)

                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   <  SRR4302536.968023/94‑1 (MQ=60)

                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                   <  SRR4302536.6646552/94‑1 (MQ=60)

                             ATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCT                                                                  <  SRR4302536.4676454/94‑1 (MQ=60)

                             ATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCT                                                                  >  SRR4302536.1772801/1‑94 (MQ=60)

                              TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCTCGATCGGGATGGGAAAACGGTCCGCAGACATGGCTCCTACCGCCCCCCT                                                                 >  SRR4302536.3786792/1‑94 (MQ=60)

                              TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTT                                                                 <  SRR4302536.5056396/94‑1 (MQ=60)

                              TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTT                                                                 <  SRR4302536.6443599/94‑1 (MQ=60)

                               GGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTG                                                                >  SRR4302536.3168995/1‑94 (MQ=60)

                               GGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGAGCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTG                                                                <  SRR4302536.6662815/94‑1 (MQ=60)

                                 TGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTT                                                              >  SRR4302536.1997160/1‑94 (MQ=60)

                                  GAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTG                                                             >  SRR4302536.792543/1‑94 (MQ=60)

                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            <  SRR4302536.3070761/94‑1 (MQ=60)

                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302536.32289/1‑94 (MQ=60)

                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302536.3685222/1‑94 (MQ=60)

                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                            >  SRR4302536.1624520/1‑94 (MQ=60)

                                    GAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTG                                                           >  SRR4302536.5617285/1‑94 (MQ=60)

                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                          <  SRR4302536.2102220/94‑1 (MQ=60)

                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                          >  SRR4302536.3060793/1‑94 (MQ=60)

                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                          >  SRR4302536.226280/1‑94 (MQ=60)

                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                          >  SRR4302536.2303197/1‑94 (MQ=60)

                                       ATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGT                                                        <  SRR4302536.210983/94‑1 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302536.6409064/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302536.4030762/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302536.393998/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302536.836827/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                      >  SRR4302536.872226/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTA                                                      >  SRR4302536.6861351/1‑94 (MQ=60)

                                         CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCACCCTTGTTGTGAGTTC                                                      >  SRR4302536.6672181/1‑94 (MQ=60)

                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                     >  SRR4302536.4923271/1‑94 (MQ=60)

                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                     >  SRR4302536.3012496/1‑94 (MQ=60)

                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                    <  SRR4302536.912706/94‑1 (MQ=60)

                                             TCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGA                                                  <  SRR4302536.1847518/94‑1 (MQ=60)

                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 >  SRR4302536.1388141/1‑94 (MQ=60)

                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 <  SRR4302536.3713467/94‑1 (MQ=60)

                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 <  SRR4302536.3393560/94‑1 (MQ=60)

                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                 <  SRR4302536.1350439/94‑1 (MQ=60)

                                               GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                                <  SRR4302536.1976947/94‑1 (MQ=60)

                                               CTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                                <  SRR4302536.1491933/94‑1 (MQ=60)

                                                 TTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCT                                              <  SRR4302536.3256338/94‑1 (MQ=60)

                                                   TCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGA                                            <  SRR4302536.2845811/94‑1 (MQ=60)

                                                     GGGATTAATCACCTGCTTTCCACGTCCGGGATGGGAAAACGGCCCCCAGACTGGGCTCATACCGCCCCCTTGTTGTGAGTTCATAAACCTGACA                                          <  SRR4302536.3617205/94‑1 (MQ=48)

                                                     GCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACA                                          >  SRR4302536.365183/1‑94 (MQ=60)

                                                     GCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACA                                          <  SRR4302536.5175284/94‑1 (MQ=60)

                                                      CGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAG                                         <  SRR4302536.6128129/94‑1 (MQ=60)

                                                      CGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAG                                         >  SRR4302536.5458519/1‑94 (MQ=60)

                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGGAAACGGTCCCCAGCCATGGCTCATACCGCCCCCTTGGTGTGAGTTTATGAACCTGACAAT                                        >  SRR4302536.1800386/1‑94 (MQ=60)

                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                        >  SRR4302536.5944130/1‑94 (MQ=60)

                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                        >  SRR4302536.906443/1‑94 (MQ=60)

                                                       AATTAATCCCCTGCATTTCACGATCGGAATGGGAAAACGGTCCCCAGCCATGCCTCATCCCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                        <  SRR4302536.6658948/94‑1 (MQ=60)

                                                        ATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTT                                       <  SRR4302536.3225200/94‑1 (MQ=60)

                                                        ATTAATCACCTGCATTCCACGATCGGGATGGGAAAACGGTCCCCAGACGTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTG                                       <  SRR4302536.1331044/94‑1 (MQ=60)

                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                     >  SRR4302536.6499373/1‑94 (MQ=60)

                                                           AATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAA                                    >  SRR4302536.3678845/1‑94 (MQ=60)

                                                           AATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAA                                    <  SRR4302536.6173620/94‑1 (MQ=60)

                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                   >  SRR4302536.3809098/1‑94 (MQ=60)

                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                   <  SRR4302536.6603272/94‑1 (MQ=60)

                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                   <  SRR4302536.4848333/94‑1 (MQ=60)

                                                             TCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGT                                  <  SRR4302536.2511033/94‑1 (MQ=60)

                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                 >  SRR4302536.540341/1‑94 (MQ=60)

                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                 >  SRR4302536.287024/1‑94 (MQ=60)

                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                 <  SRR4302536.2044340/94‑1 (MQ=60)

                                                               ACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                                >  SRR4302536.3665445/1‑94 (MQ=60)

                                                               ACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                                <  SRR4302536.2676663/94‑1 (MQ=60)

                                                                CCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATA                               <  SRR4302536.3239088/94‑1 (MQ=60)

                                                                CCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATA                               <  SRR4302536.659487/94‑1 (MQ=60)

                                                                 CTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAA                              >  SRR4302536.5012822/1‑94 (MQ=60)

                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                             <  SRR4302536.4995121/94‑1 (MQ=60)

                                                                   GCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGT                            <  SRR4302536.4832063/94‑1 (MQ=60)

                                                                     ATTTCACGATCGGGATGGGAAAACGGTCCCCAGTCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCA                          >  SRR4302536.5426717/1‑94 (MQ=60)

                                                                      TTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAG                         <  SRR4302536.1276585/94‑1 (MQ=60)

                                                                      TTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAG                         <  SRR4302536.2678760/94‑1 (MQ=60)

                                                                       TTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGC                        >  SRR4302536.4423511/1‑94 (MQ=60)

                                                                       TTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGATCAAGTATAAGTCAGC                        >  SRR4302536.4837338/1‑94 (MQ=60)

                                                                           CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCTAGTATAAGTCAGCCTGT                    >  SRR4302536.5837322/1‑94 (MQ=60)

                                                                            gaggGGGATGGGAAACCGGTCCCCAGACATGGCTCATCCCGCCCCTTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   <  SRR4302536.6218351/90‑1 (MQ=60)

                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   <  SRR4302536.6315077/94‑1 (MQ=60)

                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                   <  SRR4302536.1537235/94‑1 (MQ=60)

                                                                             ATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGA                  >  SRR4302536.5283257/1‑94 (MQ=60)

                                                                               CGGGATGGGTAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT                <  SRR4302536.2598194/94‑1 (MQ=60)

                                                                               CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT                <  SRR4302536.5968445/94‑1 (MQ=60)

                                                                               CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT                >  SRR4302536.2090385/1‑94 (MQ=60)

                                                                               CGGGATGGGAAAACGGACCCCAGACATGGCTCATACCGCCCCCTTGTGGTGAGTTCATGAACCGGACAGTTCAAGTATAAGTCAGCTTGTGATT                <  SRR4302536.3821855/94‑1 (MQ=60)

                                                                                GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA               >  SRR4302536.786921/1‑94 (MQ=60)

                                                                                GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA               <  SRR4302536.3478479/94‑1 (MQ=60)

                                                                                GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA               <  SRR4302536.6597070/94‑1 (MQ=60)

                                                                                GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA               <  SRR4302536.6019869/94‑1 (MQ=60)

                                                                                 GGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCATCTTGTGATTAT              <  SRR4302536.4193511/94‑1 (MQ=60)

                                                                                      GGAAATCGGTCCCCAGACATGGCTCATGCCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTCATTATTTTTT         <  SRR4302536.1294702/94‑1 (MQ=60)

                                                                                      GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT         <  SRR4302536.6809340/94‑1 (MQ=60)

                                                                                      GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT         <  SRR4302536.2989214/94‑1 (MQ=60)

                                                                                        AAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGT       >  SRR4302536.6077120/1‑94 (MQ=60)

                                                                                         AAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGACCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT      <  SRR4302536.5524429/94‑1 (MQ=60)

                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTC     <  SRR4302536.5080772/94‑1 (MQ=60)

                                                                                             GGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  SRR4302536.465381/1‑94 (MQ=60)

                                                                                             GGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  SRR4302536.3819231/1‑94 (MQ=60)

GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCC  >  NC_000913/2223539‑2223725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`