Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,264,802 T→C F180L (TTT→CTT)  yeiQ → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,264,8020TC97.4% 474.9 / ‑0.8 154F180L (TTT→CTT) yeiQputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (87/63);  total (90/64)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 161 total aligned reads displayed.

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891
                                                                                          |                                                                                         
cACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGctt                                                                                         >  1:4336094/1‑93 (MQ=255)
  cGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAc                                                                                       >  2:2223033/1‑93 (MQ=255)
  cGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAc                                                                                       <  2:2615476/93‑1 (MQ=255)
   gATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTAcc                                                                                      <  1:75749/93‑1 (MQ=255)
    aTCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCg                                                                                     >  1:2598454/1‑93 (MQ=255)
     tCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGt                                                                                    >  1:3711571/1‑93 (MQ=255)
      cTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAAAGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTg                                                                                   >  1:1442790/1‑93 (MQ=255)
          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  1:225456/1‑93 (MQ=255)
          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  1:2948077/1‑93 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              >  2:974213/1‑93 (MQ=255)
           aaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCtctc                                                                              >  2:467757/1‑93 (MQ=255)
            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:2210053/93‑1 (MQ=255)
            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:1613957/93‑1 (MQ=255)
             ccccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCt                                                                            <  1:1674523/93‑1 (MQ=255)
             ccccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCt                                                                            >  2:2689410/1‑93 (MQ=255)
              cccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCTAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTg                                                                           >  2:4596531/1‑93 (MQ=255)
              cccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTg                                                                           >  1:2723187/1‑93 (MQ=255)
               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          >  1:4374934/1‑93 (MQ=255)
               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          >  1:1286546/1‑93 (MQ=255)
               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTAAACCGCTTACCGTGCTCTCCTGc                                                                          <  1:834694/93‑1 (MQ=255)
                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  2:1677261/1‑93 (MQ=255)
                 tGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGa                                                                        >  1:936264/1‑93 (MQ=255)
                  gaagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAc                                                                       <  2:1040871/93‑1 (MQ=255)
                   aagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACa                                                                      >  1:4513498/1‑93 (MQ=255)
                    agacCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     <  1:3479850/93‑1 (MQ=255)
                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     >  2:2137800/1‑93 (MQ=255)
                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     >  2:55911/1‑93 (MQ=255)
                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGAGGCCTTACACCGCTTAAATTGCTCTGCTGTGAGaa                                                                     >  2:1200161/1‑93 (MQ=255)
                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:1123955/1‑93 (MQ=255)
                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    >  1:3370436/1‑93 (MQ=255)
                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   <  1:3567370/93‑1 (MQ=255)
                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   >  2:3881018/1‑93 (MQ=255)
                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  >  1:487841/1‑93 (MQ=255)
                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  <  1:143459/93‑1 (MQ=255)
                       aCCTCACTCCGCACCGGATATTCTCGTCGAAGAGCTGAAGCGCCGCCGTGAGCACGGCCTTACACCGCTTCCCGTGCTCTCCTGCGACAatat                                                                  <  2:2867087/93‑1 (MQ=255)
                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 >  2:2469734/1‑93 (MQ=255)
                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               <  1:3751897/93‑1 (MQ=255)
                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              >  2:2934871/1‑93 (MQ=255)
                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              <  2:4629977/93‑1 (MQ=255)
                            aCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             >  1:1205682/1‑93 (MQ=255)
                            aCTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCg                                                             >  1:704249/1‑93 (MQ=255)
                             cTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGa                                                            <  2:2083414/93‑1 (MQ=255)
                             cTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGa                                                            <  2:853615/93‑1 (MQ=255)
                              tCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTCCGACAATATTCCCgcc                                                           >  1:1166486/1‑91 (MQ=255)
                               ccGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGGGAGCGCGGCCTTACACCGCTTACCGTGCTGTCCTGCGACAATATTCCCcaca                                                          >  2:1303498/1‑93 (MQ=255)
                                  cACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATg                                                       >  1:3968519/1‑93 (MQ=255)
                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      <  2:3575073/93‑1 (MQ=255)
                                      gggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   >  2:2759417/1‑93 (MQ=255)
                                       gggaTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTTTCCTGCGACAATATTCCCGACAATGGTCAt                                                  <  2:3623073/90‑1 (MQ=255)
                                         tATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgt                                                <  2:1875406/93‑1 (MQ=255)
                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  2:1454514/93‑1 (MQ=255)
                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  2:1093329/93‑1 (MQ=255)
                                            tctcTTCGAAGCACTGAAACTCCGCCGTGACCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATTGTCATgtggt                                             <  2:2964705/93‑1 (MQ=255)
                                             ctcGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATgtggtg                                            <  1:1830546/93‑1 (MQ=255)
                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGa                                           <  2:2927222/93‑1 (MQ=255)
                                               cgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaa                                          >  1:189831/1‑93 (MQ=255)
                                               cgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaa                                          <  1:2076098/93‑1 (MQ=255)
                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGacaa                                        >  1:132056/1‑93 (MQ=255)
                                                 tcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaa                                        >  1:3885300/1‑93 (MQ=255)
                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  2:1537260/93‑1 (MQ=255)
                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  1:3262623/93‑1 (MQ=255)
                                                  cgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  1:3556685/93‑1 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:4272257/1‑93 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:2688163/1‑93 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:4099638/1‑93 (MQ=255)
                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      <  2:1739355/93‑1 (MQ=255)
                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgc                                    >  1:3286467/1‑93 (MQ=255)
                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGGAAAAcgc                                    >  2:1716673/1‑93 (MQ=255)
                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   <  2:1985907/93‑1 (MQ=255)
                                                      gCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgcg                                   >  1:22980/1‑93 (MQ=255)
                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  1:2473327/1‑93 (MQ=255)
                                                         cTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTg                                >  1:3693811/1‑93 (MQ=255)
                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               >  2:350405/1‑93 (MQ=255)
                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           >  1:2485402/1‑93 (MQ=255)
                                                              aCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTggg                           <  2:1212776/93‑1 (MQ=255)
                                                               cgccgcCGTGAGCGCGGCCTCACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGa                          <  2:784030/93‑1 (MQ=255)
                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  1:4523487/93‑1 (MQ=255)
                                                                gccgccGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGaa                         <  2:3648055/93‑1 (MQ=255)
                                                                  cgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATg                       >  1:4191008/1‑93 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACTATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  2:3061029/1‑93 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  1:4074380/1‑93 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      >  1:49410/1‑93 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATgg                      <  2:4381699/93‑1 (MQ=255)
                                                                   gccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGGTCTGGGAATgg                      >  2:131092/1‑93 (MQ=255)
                                                                     cgTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    >  1:1592068/1‑93 (MQ=255)
                                                                     cgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    >  1:1781926/1‑93 (MQ=255)
                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  1:3144683/1‑93 (MQ=255)
                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  2:3873134/1‑93 (MQ=255)
                                                                       tGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGa                  >  2:1402979/1‑93 (MQ=255)
                                                                        gAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGCGCTGGGAATGGCAGaa                 >  1:101620/1‑93 (MQ=255)
                                                                              ggCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGt           >  1:1046471/1‑93 (MQ=255)
                                                                              ggCCTTACACCGCTTACCGTCCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGt           <  2:239665/93‑1 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:4460576/93‑1 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:420107/93‑1 (MQ=255)
                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:2702596/93‑1 (MQ=255)
                                                                                 cTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCg        >  2:4180226/1‑93 (MQ=255)
                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  2:4419891/1‑93 (MQ=255)
                                                                                      acCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGa   >  2:4412802/1‑93 (MQ=255)
                                                                                       ccGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGaa  <  2:3377097/93‑1 (MQ=255)
                                                                                       ccGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGaa  >  1:4471137/1‑93 (MQ=255)
                                                                                          |                                                                                         
CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAAC  >  NC_000913/2264709‑2264892
                                                                                             |                                                                                          
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                            >  SRR4302544.290747/1‑94 (MQ=60)
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                            >  SRR4302544.3593035/1‑94 (MQ=60)
  TCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTT                                                                                          >  SRR4302544.4380227/1‑94 (MQ=60)
   CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTA                                                                                         <  SRR4302544.1953645/94‑1 (MQ=60)
      GATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCG                                                                                      >  SRR4302544.2623435/1‑94 (MQ=60)
      GATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCG                                                                                      <  SRR4302544.76479/94‑1 (MQ=60)
       ATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGT                                                                                     >  SRR4302544.3748490/1‑94 (MQ=60)
        TCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAAAGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTG                                                                                    >  SRR4302544.1457143/1‑94 (MQ=60)
         CTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGC                                                                                   >  SRR4302544.4085042/1‑94 (MQ=60)
            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                <  SRR4302544.2460743/94‑1 (MQ=60)
            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                >  SRR4302544.227797/1‑94 (MQ=60)
            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                >  SRR4302544.2976393/1‑94 (MQ=60)
                CCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTG                                                                            >  SRR4302544.2749409/1‑94 (MQ=60)
                CCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTG                                                                            <  SRR4302544.1691018/94‑1 (MQ=60)
                 CCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGC                                                                           >  SRR4302544.1299218/1‑94 (MQ=60)
                 CCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGC                                                                           >  SRR4302544.2761015/1‑94 (MQ=60)
                 CCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGC                                                                           >  SRR4302544.4419283/1‑94 (MQ=60)
                 CCCTGAAGAACCTCACTCCGCACCGGGGATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGC                                                                           >  SRR4302544.1221181/1‑94 (MQ=60)
                  CCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTAAACCGCTTACCGTGCTCTCCTGCG                                                                          <  SRR4302544.842915/94‑1 (MQ=60)
                   CTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGA                                                                         >  SRR4302544.945491/1‑94 (MQ=60)
                    TGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAC                                                                        >  SRR4302544.3510272/1‑94 (MQ=60)
                     GAAGAACCTCGCTCCGCACCGGGTATTCTCGTCGAAGCGCTGAATCGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACA                                                                       <  SRR4302544.3823437/94‑1 (MQ=60)
                     GAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACA                                                                       >  SRR4302544.4558715/1‑94 (MQ=60)
                       AGACCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                     <  SRR4302544.3513030/94‑1 (MQ=60)
                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                     >  SRR4302544.3402616/1‑94 (MQ=60)
                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                     >  SRR4302544.1135155/1‑94 (MQ=60)
                        GAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATA                                                                    <  SRR4302544.1187277/94‑1 (MQ=60)
                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                   <  SRR4302544.3601573/94‑1 (MQ=60)
                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                   >  SRR4302544.492618/1‑94 (MQ=60)
                          ACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATT                                                                  <  SRR4302544.144819/94‑1 (MQ=60)
                             TCCCTCCGCACCGGGTATTCTCGTCGAAGCACTGAACCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCC                                                               <  SRR4302544.3976132/94‑1 (MQ=60)
                             TCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCC                                                               <  SRR4302544.3789155/94‑1 (MQ=60)
                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                              >  SRR4302544.711254/1‑94 (MQ=60)
                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                              >  SRR4302544.1217623/1‑94 (MQ=60)
                                CTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTATACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGAC                                                            >  SRR4302544.4170878/1‑94 (MQ=60)
                                CTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTCCGACAATATTCCCGCC                                                            >  SRR4302544.1178119/1‑94 (MQ=60)
                                    GCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATG                                                        >  SRR4302544.4007990/1‑94 (MQ=60)
                                     CACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGG                                                       >  SRR4302544.1401911/1‑94 (MQ=60)
                                      aaaggtgATTCTCGACGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTGCCGTGCTCTACTGCGACAATATTCCCGACAATGGT                                                      <  SRR4302544.2633355/87‑1 (MQ=60)
                                      ACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGT                                                      >  SRR4302544.3303051/1‑94 (MQ=60)
                                             ATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGG                                               <  SRR4302544.894537/94‑1 (MQ=60)
                                               TCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTG                                             >  SRR4302544.3755349/1‑94 (MQ=60)
                                                CTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGA                                            >  SRR4302544.747079/1‑94 (MQ=60)
                                                CTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGA                                            <  SRR4302544.1848345/94‑1 (MQ=60)
                                                 TCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAA                                           <  SRR4302544.1846089/94‑1 (MQ=60)
                                                 TCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAA                                           >  SRR4302544.191722/1‑94 (MQ=60)
                                                  CGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAA                                          <  SRR4302544.2096051/94‑1 (MQ=60)
                                                   GTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGACAA                                         >  SRR4302544.133271/1‑94 (MQ=60)
                                                   GTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAA                                         >  SRR4302544.3923887/1‑94 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                       >  SRR4302544.640056/1‑94 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                       >  SRR4302544.4140436/1‑94 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                       <  SRR4302544.3590771/94‑1 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                       <  SRR4302544.3293782/94‑1 (MQ=60)
                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                       >  SRR4302544.2713938/1‑94 (MQ=60)
                                                       AAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGC                                     >  SRR4302544.3317782/1‑94 (MQ=60)
                                                        AGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCG                                    >  SRR4302544.23204/1‑94 (MQ=60)
                                                        AGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCG                                    <  SRR4302544.1665410/94‑1 (MQ=60)
                                                          CACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                  >  SRR4302544.2496975/1‑94 (MQ=60)
                                                          CACTGAAACGCCCCCGTGAGCGCGGCCCTACAGCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                  <  SRR4302544.2111764/94‑1 (MQ=60)
                                                           ACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTG                                 >  SRR4302544.3730613/1‑94 (MQ=60)
                                                              GAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTG                              <  SRR4302544.267774/94‑1 (MQ=60)
                                                               AAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGG                             >  SRR4302544.1075322/1‑94 (MQ=60)
                                                                AACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGG                            >  SRR4302544.2509200/1‑94 (MQ=60)
                                                                AACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGG                            <  SRR4302544.4675988/94‑1 (MQ=60)
                                                                AACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGG                            >  SRR4302544.2509680/1‑94 (MQ=60)
                                                                  CGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAA                          >  SRR4302544.3562052/1‑94 (MQ=60)
                                                                   GCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAAT                         <  SRR4302544.4568766/94‑1 (MQ=60)
                                                                    CCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATG                        >  SRR4302544.4232514/1‑94 (MQ=60)
                                                                    CCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATG                        >  SRR4302544.4541132/1‑94 (MQ=60)
                                                                     CGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGG                       >  SRR4302544.4114929/1‑94 (MQ=60)
                                                                     CGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGG                       >  SRR4302544.49907/1‑94 (MQ=60)
                                                                       CCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCA                     >  SRR4302544.1607761/1‑94 (MQ=60)
                                                                       CCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCA                     >  SRR4302544.1799353/1‑94 (MQ=60)
                                                                        CGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAG                    >  SRR4302544.4497268/1‑94 (MQ=60)
                                                                         GTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGA                   >  SRR4302544.3174582/1‑94 (MQ=60)
                                                                          TGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGCGCTGGGAATGGCAGAA                  >  SRR4302544.102550/1‑94 (MQ=60)
                                                                                CGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGT            >  SRR4302544.1056849/1‑94 (MQ=60)
                                                                                 GGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTT           <  SRR4302544.1466613/94‑1 (MQ=60)
                                                                                   CCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCG         <  SRR4302544.3138709/94‑1 (MQ=60)
                                                                                         ACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA   >  SRR4302544.4516102/1‑94 (MQ=60)
                                                                                         ACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA   >  SRR4302544.3649464/1‑94 (MQ=60)
                                                                                          CCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAAC  >  SRR4302544.4088409/1‑94 (MQ=60)
                                                                                             |                                                                                          
ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAAC  >  NC_000913/2264709‑2264892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑